Techniques microdialysis. Medical search. Frequent questions

This study was performed to evaluate quantitatively the brain distribution and the efflux transport across the blood-brain barrier of probenecid, using in vivo microdialysis and in situ brain perfusion techniques. (aspetjournals.org)
In vivo microdialysis analyses revealed an increase in the activity of VLPO astrocytes and ATP levels during sleep states ( n = 4). (jneurosci.org)
We examined the effect of three months of rosiglitazone treatment (4 mg b.i.d.) on whole-body insulin sensitivity and in vivo peripheral adipocyte insulin sensitivity as assessed by glycerol release in microdialysis from subcutaneous fat during a two-step (20 and 120 mU · m −2 · min −1 ) hyperinsulinemic-euglycemic clamp in nine type 2 diabetic subjects. (diabetesjournals.org)
Here, we combined two techniques - glucose clamps and in vivo microdialysis - as a means to dynamically modulate blood glucose levels in awake, freely moving mice while measuring real-time changes in amyloid-β (Aβ), glucose, and lactate within the hippocampal interstitial fluid (ISF). (jci.org)
Finally, in vivo microdialysis was performed in freely moving rats to evaluate the effect of acute MXE administration (0.125, 0.25 and 0.5 mg/kg IV) on dopamine release in the NAc shell. (erowid.org)
Positron emission tomography (PET) is a powerful imaging technique which enables in vivo examination of brain functions. (bmj.com)
A technique using parallel administering and collecting microdialysis fibers was developed to estimate the in vivo concentration of K + outside the administering fiber. (utmb.edu)
From anesthetized rats in the 90s to conscious wild-type or knockout mice today, intracerebral in vivo microdialysis provided important information about the brain mechanism of action of psychotropic drugs such as antidepressants. (eurekaselect.com)

The microdialysis technique requires the insertion of a small microdialysis catheter (also referred to as microdialysis probe) into the tissue of interest. (wikipedia.org)
The microdialysis probe is designed to mimic a blood capillary and consists of a shaft with a semipermeable hollow fiber membrane at its tip, which is connected to inlet and outlet tubing. (wikipedia.org)
Due to the constant perfusion of the microdialysis probe with fresh perfusate, a total equilibrium cannot be established. (wikipedia.org)
In theory, the extraction efficiency of a microdialysis probe can be determined by: 1) changing the drug concentrations while keeping the flow rate constant or 2) changing the flow rate while keeping the respective drug concentrations constant. (wikipedia.org)
CMA 7 55 kDa Microdialysis Probe, 1 mm membrane length, pkg. (harvardapparatus.com)
CMA 8 High Cut-Off Microdialysis Probe, 1 mm membrane length (pkg. (harvardapparatus.com)
We recommend the use of the CMA Microdialysis CNS Perfusion fluid with Dextran 500 kDa 3% in order to stabilize the pressure within the probe and limit ultra-filtration. (harvardapparatus.com)
First, adult male Wistar rats with a microdialysis probe aimed at the PVN or the supraoptic nucleus were exposed to either a dominant male rat (social defeat) or a novel cage. (jneurosci.org)
A microdialysis membrane probe of 100 kDa molecular weight cut-off was employed and in situ calibration methods with phenol red and fluorescent 40 kDa dextran were developed to measure the relative recovery of the solute of interest, and membrane fouling, respectively. (ox.ac.uk)
This review compares microdialysis, microperfusion, and implanted EEG probe architectures and performance parameters. (elsevierpure.com)
Modelling domain of these mathematical models would focus on the microdialysis probes, the membrane attached to the probes, and the probe surrounding area (PSA). (usm.my)
For instance, microdialysis sampling of glucose under perfused solution flow rate of 1.0 μL min-1 using commercially available liner probe and concentric probe (10 mm membrane length, 30 kDa molecular weight cut-off) yield recovery of 30.98% and 36.67%, respectively. (usm.my)
In addition, although microdialysis is traditionally defined as a diffusion-limited process, it is shown in this work that under combinations of high perfused solution flow rate in the probe, high membrane porosity, and large membrane pore diameter, the influence of convective flux on the recovery of microdialysis sampling would be significant. (usm.my)

We first began developing the application of microdialysis during Olivier Humphrey's PhD (completed in 2019) at the Centre for Environmental Geochemistry (BGS/University of Nottingham), where he was investigating Short-term iodine soil interactions following a simulated natural/anthropogenic iodine addition event (full paper). (bgs.ac.uk)

Rapid online-SPE-MS/MS method for ketoprofen determination in dermal interstitial fluid samples from rats obtained by microdialysis or open-flow microperfusion. (joanneum.at)
Microdialysis in freely moving rats is a new technology that allows researchers to implant measuring devices in the rat brain. (drugscience.org)

The technique known as microdialysis currently stands as one of the more minimally invasive sampling options for testing the impact of drugs on brain tumors, but even that requires an entire catheter to be inserted into the patient's skull cavity. (yahoo.com)
A microdialysis catheter was placed intraperitoneally in each patient. (biomedcentral.com)

Microdialysis is a minimally-invasive sampling technique that is used for continuous measurement of free, unbound analyte concentrations in the extracellular fluid of virtually any tissue. (wikipedia.org)

Brain microdialysis is used to determine the factors/neurotransmitters that are released from neurons in the course of neuronal communication. (edu.au)
Standard electrophysiological techniques were used to record changes in firing activities of ventral tegmental area (VTA) dopamine neurons projecting to the nucleus accumbens (NAc) shell after acute injection of cumulative doses of MXE (0.031-0.5 mg/kg IV). (erowid.org)
The effects of MDMA (3,4-methylenedioxymethamphetamine) on the activity of serotonin (5-HT)-containing dorsal raphe neurons were characterized using extracellular single-unit recording and microdialysis techniques in the in vitro midbrain slice preparation. (erowid.org)

Microdialysis results showed that patients with upper gastrointestinal tract lesions had significantly higher levels of postoperative intraperitoneal glucose and glycerol concentrations, as well as lower lactate/pyruvate ratios and lactate/glucose ratios. (biomedcentral.com)

Monitoring Neurochemistry in Traumatic Brain Injury Patients Using Microdialysis Integrated with Biosensors: A Review. (cam.ac.uk)
Liu, D & Mcadoo, DJ 1993, ' An Experimental Model Combining Microdialysis with Electrophysiology, Histology, and Neurochemistry for Exploring Mechanisms of Secondary Damage in Spinal Cord Injury: Effects of Potassium ', Journal of neurotrauma , vol. 10, no. 3, pp. 349-362. (utmb.edu)
Mcadoo, David J. / An Experimental Model Combining Microdialysis with Electrophysiology, Histology, and Neurochemistry for Exploring Mechanisms of Secondary Damage in Spinal Cord Injury : Effects of Potassium . (utmb.edu)

While water-soluble compounds generally diffuse freely across the microdialysis membrane, the situation is not as clear for highly lipophilic analytes, where both successful (e.g. corticosteroids) and unsuccessful microdialysis experiments (e.g. estradiol, fusidic acid) have been reported. (wikipedia.org)
In our lab, we use microdialysis in freely moving animals, which allows the correlation of disease-related behaviours with ongoing neurochemical changes in the brain. (edu.au)
When the rat recovers from the surgery, researchers are able to use the microdialysis device to measure changes in dopamine levels in a living, "freely moving" specimen. (drugscience.org)

Clinical microdialysis enables monitoring of the cerebral extracellular chemistry of neurosurgical patients. (thejns.org)
A microdialysis technique was used to sample AAs in the extracellular fluid of the DH apex in eight patients in whom surgery in the dorsal root entry zone (DREZ) was performed. (thejns.org)
We concluded that microdialysis could be used to sample a wide range of molecular species released during cell metabolism and extracellular matrix turnover, which were direct or indirect indications of cell and tissue functions. (ox.ac.uk)
In a traumatically injured brain, the cerebral microdialysis technique allows continuous sampling of fluid from the brain's extracellular space. (cam.ac.uk)
Skin microdialysis (SMD) is a versatile sampling technique that can be used to recover soluble endogenous and exogenous molecules from the extracellular compartment of human skin. (biomedcentral.com)
Microdialysis is a well-known sampling technique in medical researches, most commonly used to measure the concentration of chemicals in the extracellular space of tissues. (usm.my)

Pharmacokinetic studies of topical ketoprofen formulations using continuous sampling techniques such as microdialysis (MD) or open-flow microperfusion (OFM) require sensitive assays due to small sample volumes. (joanneum.at)
Much of my research uses a novel sampling technique called 'microdialysis' which affords a continuous small-scale view of solute fluxes in soil. (slu.se)
To address this, recent advances have focussed on developing biosensing techniques for integration with microdialysis to achieve continuous online monitoring. (cam.ac.uk)
Intraperitoneal microdialysis (IPM) makes it is possible to obtain continuous monitoring of the intraabdominal condition by measuring several parameters as markers for inflammation or ischaemia. (biomedcentral.com)

Finally, previously developed microdialysis electrodes and microperfusion electrodes are reviewed in preclinical and clinical settings. (elsevierpure.com)
Critically, current and precedent microdialysis and microperfusion probes lack the ability to collect neurochemical data that is spatially and temporally coincident with EEG data derived from depth electrodes. (elsevierpure.com)

Before making microsurgical lesions, specific concentric-type microdialysis probes were implanted over a 60-minute period in the DREZ and directed to the DH apex (10 implantations). (thejns.org)

In a parallel series of experiments, microdialysis probes resting on the brain slice surface provided a means to estimate 5-HT release from the dorsal raphe nucleus. (erowid.org)
This hypothesis is based partially on results of microdialysis experiments performed in naïve, non stressed Rodents. (eurekaselect.com)

BGS's Inorganic Geochemistry Facility is at the forefront of developing microdialysis as a soil sampling technique, with the ability to assess rapid chemical reactions with unparalleled temporal and spatial resolution without significantly disturbing in situ physicochemical soil properties. (bgs.ac.uk)
Microdialysis as an in situ technique for sampling soil enzymes. (slu.se)
Improving in situ recovery of soil nitrogen using the microdialysis technique. (slu.se)

3) Can microdialysis be used to distinguish dopamine release upon an animal's receiving a reward from dopamine release upon the animal's receiving a cue associated with the reward? (grantome.com)
The principle of microdialysis technique is based on the balance between the release of neurotransmitters (e.g., serotonin 5-HT, norepinephrine, dopamine) and re-uptake by selective transporters (e.g. (eurekaselect.com)

The use of probes with large pore size membranes required for the microdialysis of large molecules creates some methodological challenges such as protein aggregation, protein adhesion, ultrafiltration and low fluid recovery/extraction efficiency. (harvardapparatus.com)

Complementary to electrophysiology, this technique reflects presynaptic monoamines release and intrasynaptic events corresponding to ≈ 80% of whole brain tissue content. (eurekaselect.com)

Two adjacent microdialysis catheters that were identical in membrane length, perfusion fluid, and flow rate showed equivalent results. (thejns.org)
Contemporary biomarker collection techniques in blood and cerebrospinal fluid have to date offered only modest clinical insights into neurologic diseases such as epilepsy and glioma. (elsevierpure.com)
Although there are already several mathematical modelling works on microdialysis, those works would focus only on providing accurate estimations of the recovery, while other features such as fluid flows are neglected. (usm.my)
The main objective of this research work is to develop finite element mathematical models that could provide accurate simulations of concentration and fluid flow profiles for microdialysis. (usm.my)

Microdialysis - originally developed for use in neuroscience and pharmacokinetics - is a new technique that uses small probes to sample compounds dissolved in soil solution (Figure 1), without the need to dig up soil and potentially alter its chemical properties. (bgs.ac.uk)

The use of the microdialysis technique for the recovery of large molecules is of increasing interest as it offers the opportunity to identify patterns of protein expression in a variety of tissue spaces as well as to evaluate useful biomarkers of disease. (harvardapparatus.com)
Using these techniques, we examine tissue from both patients and various mouse and rat models for certain diseases. (edu.au)
Applying many of the above techniques to h uman post-mortem brain tissue from people suffering disease, allows us to understand what processes are disrupted in the diseased brain. (edu.au)
Microdialysis for monitoring the process of functional tissue culture. (ox.ac.uk)
In this paper, microdialysis is adopted to monitor functional tissue growth in a bioreactor. (ox.ac.uk)
Microdialysis - Monitoring Tissue Chemistry in Intensive Care Medicine. (lu.se)
The predictability of root coverage procedures was dramatically increased by the use of subepithelial connective tissue graft techniques 6 . (bvsalud.org)

Despite technical and analytical limitations related to the intraoperative conditions, this technique offers new possibilities for clinical research on neurotransmitters involved in some relevant pathological states, especially in chronic pain and spasticity. (thejns.org)
The other key element of this treatment technique is exposure, and since it is often difficult to achieve in clinical settings [7] , the imaginary exposure is commonly used. (hpathy.com)
We utilize a variety of basic, translational, and clinical experimental techniques to investigate these hypotheses. (wustl.edu)

The direction of the analyte flow is determined by the respective concentration gradient and allows the usage of microdialysis probes as sampling as well as delivery tools. (wikipedia.org)
Finally, a mixture of a V1 AVP and the α-helical corticotropin-releasing hormone (CRH) receptor antagonists administered via inverse microdialysis into the PVN caused a significant increase in the plasma adrenocorticotropic hormone (ACTH) concentration compared with vehicle-treated controls both under basal conditions and during social defeat, indicating inhibitory effects of intra-PVN-released AVP and/or CRH on HPA system activity. (jneurosci.org)

Matthew Ogley, a placement student from the University of Surrey, is currently working in the Inorganic Geochemistry facility to support the research efforts being made on microdialysis, as well as undertaking routine analyses in the laboratories. (bgs.ac.uk)

The microdialysis principle was first employed in the early 1960s, when push-pull canulas and dialysis sacs were implanted into animal tissues, especially into rodent brains, to directly study the tissues' biochemistry. (wikipedia.org)

There are a number of techniques capable of collecting and measuring dissolved compounds in water within soil, but the complex nature of soil demands methods that can collect samples faster and at smaller scales without disturbing the natural chemistry of the soil. (bgs.ac.uk)
We have since been awarded a Royal Society of Chemistry grant to continue this research, and have developed a project aimed at establishing harmonised methods for both microdialysis sampling in soils and subsequent data interpretation. (bgs.ac.uk)
Despite the potential advantages of microdialysis, considerable effort is required to develop and progress the analytical chemistry and theoretical frameworks to apply the technology to soil solution multi-elemental analysis. (bgs.ac.uk)
We aim to establish microdialysis as a tool capable of assessing fine-scale, rapid soil chemistry interactions to better inform existing geochemical models that influence phytoremedial and crop biofortification strategies globally, and look forward to updating you in the future as this exciting technique is developed further. (bgs.ac.uk)

At two weeks post-injury pyruvate and lactate concentrations were assessed in both the injured and uninjured limbs using microdialysis followed by enzymatic quantification. (nih.gov)
A complementary technique, fast scan cyclic voltammetry measures changes in concentrations over the sub-second to several second timeframe. (grantome.com)

The proper selection of an appropriate calibration method is critically important for the success of a microdialysis experiment. (wikipedia.org)

We further used microdialysis technique to study the underlying mechanisms for the impaired MDMA reward produced by the12-week exercise pre-exposure. (erowid.org)

New techniques in soil sampling could be the key to alleviating hidden hunger. (bgs.ac.uk)
The use of microdialysis to investigate soil-elemental speciation dynamics has been further developed by Elliott Hamilton during his PhD (completed in 2020), which will be summarised in a future blog. (bgs.ac.uk)
The influence of sucrose on soil nitrogen availability - A root exudate simulation using microdialysis. (slu.se)
Microdialysis in soil environments: Current practice and future perspectives. (slu.se)
My research focuses on an emerging soil sampling technology called microdialysis . (scottbuckley.com.au)
Microdialysis-a new technology for investigating soil nitrogen fluxes in the rhizosphere, 7th International Nitrogen Initiative Conference , Melbourne, Australia. (scottbuckley.com.au)

The molecular weight cutoff of commercially available microdialysis probes covers a wide range of approximately 6-100kD, but also 1MD is available. (wikipedia.org)

This report considers how a range of neuroimaging techniques including functional magnetic resonance imaging, magnetoencephalography and positron emission tomography, singly and combined, can address human pain conditions. (ox.ac.uk)
The purpose of the study was to (1) identify the main risk factors for local recurrence, (2) evaluate the recurrence-free survival in dependence on neoadjuvant denosumab use and the type of procedure, and (3) compare the functional outcomes after curettage and en bloc resection. (bvsalud.org)
The functional outcome of patients after curettage was better compared to en bloc resection. (bvsalud.org)

The present study aimed to assess microdialysis in this setting. (biomedcentral.com)

In order to resolve this issue, it is necessary to understand the mass transport limitations of microdialysis set-up, by scrutinizing how each operational and design parameters of the microdialysis set-up affect the recovery. (usm.my)
Using these mathematical models, the advantages and drawbacks of altering the operational and design parameters for microdialysis set-up were scrutinized. (usm.my)

CMA offers new complete solutions for the study of large molecules using the microdialysis techniques. (harvardapparatus.com)
The present study demonstrates that microdialysis can be performed safely in the human DH during DREZ lesioning. (thejns.org)
The fellowship allows Dr Di Pietro to combine neuroimaging techniques available in the US with the results of the five years microRNA study. (birmingham.ac.uk)

Since these findings didn't show these techniques to be ineffective, just that there is no connection to meridians, we've had pursued this path. (hpathy.com)

While these techniques had a number of experimental drawbacks, such as the number of samples per animal or no/limited time resolution, the invention of continuously perfused dialytrodes in 1972 helped to overcome some of these limitations. (wikipedia.org)

Why large molecules in microdialysis research? (harvardapparatus.com)
And my luck was that I entered brain research at the time, in the late 1960s, when modern neuroscience was born, and in the labora- tory where a new groundbreaking microscopic technique, the Falck-Hillarp monoamine histofluorescence method, had just been developed. (lu.se)

There has been an almost three decades long discussion in psychology on the effectiveness of meridian therapies, but even the results of their most vocal critics have found that these techniques are effective only not because of use of the meridians. (hpathy.com)

[ 3 , 4 ] Further refinements in CPB hardware and techniques, perfusion methods, myocardial and brain protection over the past 2 decades contributed to improved outcomes of surgical treatment of CHD. (medscape.com)

The recovery can be determined at steady-state using the constant rate of analyte exchange across the microdialysis membrane. (wikipedia.org)
CMA offers a set of microdialysis probes for the recovery of large molecules (such as proteins, cytokines, growth factors, antibody and neuropeptides…) on awake rodents. (harvardapparatus.com)

The technique, as powerful as it is, has one major limitation - it cannot measure rapid changes that are relevant to phenomena such as spreading depolarization and behavior. (grantome.com)
In this case, convection-enhanced diffusion equations are necessary to represent the mass transport phenomena across the membrane attached to microdialysis probes. (usm.my)

Introduction of the technique into different hospitals' neurosurgical units has resulted in variations in the method of application. (thejns.org)

Based on our sound theoretical insight and technical improvements, we propose to improve the timescale of microdialysis to cover this important range. (grantome.com)

Microdialysis probes can consequently be calibrated by either measuring the loss of analyte using drug-containing perfusate or the gain of analyte using drug-containing sample solutions. (wikipedia.org)
Microdialysis was used to administer candidate damaging agents and to sample the release of other substances in response to administered agents. (utmb.edu)

Microdialysis - a sensitive method for estimating plant-available N released during litter decomposition, 7th International Nitrogen Initiative Conference , Melbourne, Australia. (scottbuckley.com.au)

Anatomy12

Organisms3

Diseases1

Chemicals and Drugs26

Analytical, Diagnostic and Therapeutic Techniques and Equipment9

Psychiatry and Psychology3

Phenomena and Processes10

Health Care2

Vivo8

Probe13

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Rats2

Catheter2

Minimally-invasive1

Neurons3

Glycerol1

Neurochemistry3

Freely3

Extracellular6

Continuous4

Microperfusion2

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