• The field of circulating tumor cell (CTC) enrichment has seen many emerging technologies in recent years, which have resulted in the identification and monitoring of clinically relevant, CTC-based biomarkers that can be analyzed routinely without invasive procedures. (oaepublish.com)
  • The use of circulating tumor cells (CTCs) as a "liquid biopsy" to interrogate the molecular profile of single patients is extremely encouraging, as these cells originate from primary tumor and metastatic sites, providing a comprehensive fingerprint of a given tumor at any time during the course of the disease with a simple blood draw [ 7 ] . (oaepublish.com)
  • Cancer stem cells (CSCs) are thought to be a source of tumor recurrence due to their stem cell-like properties. (biomedcentral.com)
  • The field of investigation of TNT-mediated tumor-stromal, and tumor-tumor, cell-cell communication is gaining momentum. (frontiersin.org)
  • It ranges from a well-differentiated tumor with EPITHELIAL CELLS indistinguishable from normal HEPATOCYTES to a poorly differentiated neoplasm. (lookformedical.com)
  • A lesion with cytological characteristics associated with invasive carcinoma but the tumor cells are confined to the epithelium of origin, without invasion of the basement membrane. (lookformedical.com)
  • A malignant epithelial tumor with a glandular organization. (lookformedical.com)
  • A cell line derived from cultured tumor cells. (lookformedical.com)
  • The efficacy of every anticancer agent is usually assessed by its ability to kill tumor cells without damaging normal tissues. (researchsquare.com)
  • Approximately 30% of dogs with transitional cell carcinoma release tumor cells in the urine so the illness can sometimes be detected through urinalysis alone. (petinsurancequotes.com)
  • This non-invasive technique uses a urine sample from the dog to diagnose this type of tumor even in its early stages when the dog is not showing any symptoms and no tumor is detected on any imaging test. (petinsurancequotes.com)
  • Unlike other cancer types, surgery is often not an option with transitional cell carcinoma because it's almost impossible to completely excise the tumor. (petinsurancequotes.com)
  • Recently, more and more evidence indicates that epithelial-to-mesenchymal transition (EMT) is an important mechanism taking place during tumor invasion and metastasis, but the molecular pathways underlying EMT have not been clearly established. (chksignal.com)
  • Different regions of oral squamous cell carcinoma (OSCC) have particular histopathological and molecular characteristics limiting the standard tumor−node−metastasis prognosis classification. (nature.com)
  • Oral squamous cell carcinoma (OSCC) is the most common type of head and neck malignant tumor and is ranked the eighth leading cause of cancer worldwide. (nature.com)
  • For example, the epithelial−mesenchymal transition phenotype presents more cells with a lower degree of differentiation and greater cell dissociation in the ITF than in other tumor areas 14 , 15 . (nature.com)
  • The presence of neoplastic islands, classified as large or small according to the number of cells in the ITF, has been described as the most aggressive pattern compared to tumors with a more uniform growth pattern, as tumor invasion occurs in a more widespread manner as cellular islands or single cells 4 . (nature.com)
  • About 95% of UTIs occur when bacteria ascend the urethra to the bladder and, in the case of pyelonephritis, ascend the ureter to the kidney. (msdmanuals.com)
  • It surrounds part of urethra, which carries urine out of bladder and through penis. (medscape.com)
  • Vimentin is a developmentally regulated intermediate filament protein (IFP) found in cells of mesenchymal origin. (thermofisher.com)
  • Then, we used Western blotting and immunofluorescent staining to detect the cellular expression of epithelial markers and mesenchymal markers. (chksignal.com)
  • BACKGROUND/AIM: The interaction of integrin αvß8 with type I collagen was shown to promote oral squamous cell carcinoma (SCC) cell proliferation via the mitogen-activated protein kinase/extracellular signal-regulated kinase pathway. (bvsalud.org)
  • CAFs maintain their phenotype for numerous passages during culture in vitro without exposure to cancer cells, while NFs cannot be infinitely proliferous like cancer cells ( 10 ). (spandidos-publications.com)
  • Ziolkowska and co-workers obtained 2D OCT images of healthy skin, actinic skin lesions with different degrees of dysplasia, and basal-cell carcinoma lesions from 10 patients. (optics.org)
  • The adherent human epidermoid carcinoma cell line A431 was suspended in 2× Happy Cell® Advanced Suspension Medium. (valelifesciences.com)
  • Carcinoma squamous cell. (lookformedical.com)
  • A carcinoma derived from stratified SQUAMOUS EPITHELIAL CELLS. (lookformedical.com)
  • An anaplastic, highly malignant, and usually bronchogenic carcinoma composed of small ovoid cells with scanty neoplasm. (lookformedical.com)
  • It represents a large group of epithelial lung malignancies which can be divided into two clinical groups: SMALL CELL LUNG CANCER and NON-SMALL-CELL LUNG CARCINOMA . (lookformedical.com)
  • A431 human epithelial carcinoma cells were either left untreated or treated with 200 ng/mL recombinant human (rh) EGF (Catalog # 236-EG) for 5 minutes. (bnwax.com)
  • Cisplatin is a chemotherapy drug used to treat several cancer types such as bladder cancer, head and neck cancer, esophageal cancer, lung cancer, mesothelioma, brain tumors, and neuroblastoma. (springeropen.com)
  • 2016 ). This study was conducted to analyze the anticancer effects of green-synthesized AgNPs using Juniperus polycarpos on A549 (adenocarcinomic human alveolar basal epithelial cells) cell lines, as well as comparing it with Cisplatin commercial drug. (springeropen.com)
  • Built upon scDiffCom, scAgeCom is an atlas of age-related cell-cell communication changes covering 23 mouse tissues from 58 single-cell RNA sequencing datasets from Tabula Muris Senis and the Calico murine aging cell atlas. (nature.com)
  • In 2004, the various tracheal substitutes and techniques of reconstruction were classified by Grillo into five categories: foreign materials, nonviable tissues, tracheal allotransplantation, autogenous tissues and tissue engineering [ 6 ]. (biomedcentral.com)
  • Many tissues and cell types are involved. (powershow.com)
  • A malignant neoplasm made up of epithelial cells tending to infiltrate the surrounding tissues and give rise to metastases. (lookformedical.com)
  • Cells from various human tissues with the body communicate by way of the secretion of EVs into proximal physique fluids. (gardos-channel.com)
  • Ultrasound generally provides better visuals on the bladder and the surrounding tissues but it costs more than a radiograph. (petinsurancequotes.com)
  • n\nMaterials and methods: A total of 169 tissues, consisting of 147 primary bladder cancers and 22 adjacent normal tissues were included compound inhibitor in this study. (chksignal.com)
  • A) Two trophozoites of T vaginalis obtained from in vitro culture, stained with Giemsa. (medscape.com)
  • Cultured hippocampal neurons after 23 days in vitro, immunostained for MAP2, a microtubule associated protein localized to dendrites (red), and PSD95, a scaffolding protein concentrated in the postsyn. (cellimagelibrary.org)
  • Chollangi T, Clabault H, Thibeault A, Yong HE, Narula S, Menkhorst E, Sanderson JT, Vaillancourt C, Murthi P (2018) An electrical impedance-based assay to examine functions of various placental cell types in vitro. (hudson.org.au)
  • Jarde T, Kerr G, Akhtar R, Abud HE (2018) Modelling intestinal carcinogenesis using in vitro organoid cultures. (hudson.org.au)
  • The application of hydrogels as a matrix for 3-dimensional cell cultures has become an indispensable tool in tissue engineering, biotechnology and biomedical research due to the improved functionality and viability of the in vitro biological system. (heraldopenaccess.us)
  • We aim to develop an HPLC-UV method, common technique for analysis of pharmaceuticals, for quantification of silibinin in the aqueous samples in order to quantification of its concentration in in vitro experiments of new formulations. (researchsquare.com)
  • Further, this study elucidated the cellular and molecular mechanisms of nanoparticles for anti-proliferative and apoptotic effects on human lung cancer cells (A549) and compared them with commercial drug cisplatin. (springeropen.com)
  • TNT cellular networks are upregulated between cancer and stromal cells under hypoxic and other conditions of physiologic and metabolic stress. (frontiersin.org)
  • The combination of 3-dimensional hydrogel cell cultures with microchip technology further allows (i) spatial and temporal control of cell growth, (ii) application of defined mechanical (e.g. shear, strain, stretch) and chemical (e.g. gradients) stimuli, as well as (iii) monitoring of dynamic cellular responses using integrated sensing strategies. (heraldopenaccess.us)
  • The main advantage of hydrogels for microfluidic cell cultures, however, is their mimicry of extracellular matrix structures including adequate porosity for cellular organization, biocompatibility, and representative stiffness, all key parameters that promote native-like tissue function. (heraldopenaccess.us)
  • The cells produced collagen type IV and the cellular form of fibronectin. (shengsci.com)
  • The prostate is located in the pelvis and is in continuity with the base of the bladder superiorly and the striated external urethral spincter inferiorly. (medscape.com)
  • Prostate-specific antigen (PSA) is a protein produced by normal prostate cells. (medscape.com)
  • The aim of this work was to study the protective activity of a honey of multifloral origin, standardized for total antioxidant power and analytically profiled (HPLC-MS) in antioxidants, in a cultured endothelial cell line (EA.hy926) subjected to oxidative stress. (researchgate.net)
  • Experiments with endothelial cells fortified with the isolated fraction from native honey enriched in antioxidants, exposed to peroxyl radicals from 1,1-diphenyl-2-picrylhydrazyl (AAPH, 10 mM) and to hydrogen peroxide (H2O2, 50-100 microM), indicated that phenolic acids and flavonoids were the main causes of the protective effect. (researchgate.net)
  • Both polarized epithelial and endothelial cells function to prevent pathogen access to the interstitium, CVBs have developed strategies to subvert these barriers in order to promote their access (Bozym et al. (biotechpatents.org)
  • Human brain microvascular endothelial cells (HBMEC), symbolize an model of the blood-brain barrier (BBB). (biotechpatents.org)
  • Interestingly, Cai2+ release is definitely involved in mediating CVB access into primary human being Rabbit Polyclonal to OR1L8 aortic endothelial cells, but is not required for CVB access into polarized epithelial cells, suggesting the intracellular signaling molecules hijacked by CVB to facilitate access are distinct between the endothelium and epithelium. (biotechpatents.org)
  • According to Smith, Clinically, the BTV seems to present as underlying endothelial cell damage resulting in a vasculitis causing edema and eventually necrosis of epithelial and mucosal surfaces. (purdue.edu)
  • Furthermore, they can connect malignant cells to benign cells, including vascular endothelial cells. (frontiersin.org)
  • Imatinib inhibits VEGF-independent angiogenesis by targeting neuropilin 1-dependent ABL1 activation in endothelial cells. (bnwax.com)
  • Breast cancer cells condition lymphatic endothelial cells within pre-metastatic niches to promote metastasis. (bnwax.com)
  • The inflammatory kinase MAP4K4 promotes reactivation of Kaposi's sarcoma herpesvirus and enhances the invasiveness of infected endothelial cells. (bnwax.com)
  • CAFs provide cancer cells with nutrition and promote the proliferation, invasion and metastasis of cells ( 6 - 9 ). (spandidos-publications.com)
  • Overall, the results revealed that the green-synthetized AgNPs had anti-metastasis and anti-proliferation effects on lung cancer cells in comparison to cisplatin with lower side effects on the normal cell line. (springeropen.com)
  • Although it is believed that anticancer properties of Silibinin are related to its effects on cell proliferation, apoptosis, inflammation, angiogenesis, and metabolism, its accurate biochemical anticancer mechanism is still under investigation (4-12). (researchsquare.com)
  • By contrast, the 10 downregulated genes were primarily associated with epithelial membrane proteins ( 11 ). (spandidos-publications.com)
  • Cancer Spheroids were successfully cultured Cells in Happy Cell ASM® in both normoxic and hypoxic conditions. (valelifesciences.com)
  • The physical examination should include palpation of the bladder and examination of external genitalia. (msdvetmanual.com)
  • In this study the researchers "investigated suspension organoid culture without scaffolds for easier harvesting and assays. (valelifesciences.com)
  • Oral SCC cell motility was investigated using modified Boyden chamber assays. (bvsalud.org)
  • Ras homolog family member A (RHOA), Ras-related C3 botulinum toxin substrate 1 (RAC1), and cell division control protein 42 homolog (CDC42) activity of oral SCC cells was analyzed by pull-down assays. (bvsalud.org)
  • In addition, in vivo serial transplantation assays, dye exclusion assays, and isolation via cell surface specific antigen profile methods are now used to identify CSCs. (biomedcentral.com)
  • More recent research applications include microchips for genomics, proteomics and cell-based assays. (heraldopenaccess.us)
  • The integrity of limited junctions facilitating bacterial transmigration across polarized airway epithelial cells, similar to the case with replicting rhinoviruses was found to be caused by poly(I:C), i.e. by double stranded RNA. (biotechpatents.org)
  • Many abnormalities of the urinary system can be diagnosed from the signalment, history and physical examination findings, serum chemistry profile, urinalysis, and aerobic bacterial urine culture. (msdvetmanual.com)
  • AgNPs are utilized for treating different types of cancerous cells due to their extraordinary biomedical features (anti-cancer, anti-bacterial, anti-viral, and anti-angiogenic properties) as well as surface-specific characteristics (Burdușel et al. (springeropen.com)
  • The main diagnostic criterion for CBP is positive bacterial cultures of prostatic fluid. (medscape.com)
  • However, some patients may have bacterial infection despite negative urine cultures. (medscape.com)
  • In a bacterial culture, a sterile sample of the urine is plated in a growth medium to isolate and identify possible bacteria in the urine. (petinsurancequotes.com)
  • Cancer stem cells (CSCs) represent a distinct group of cells within cancerous tissue that possess the ability to initiate tumorigenesis and exhibit potency, self-renewal, and drug resistance. (bmrat.org)
  • The study of CSCs often encounters challenges in obtaining these cells of interest or generating a sufficient quantity for downstream analysis. (bmrat.org)
  • MicroRNAs (miRNAs) regulate both normal stem cells and CSCs, and dysregulation of miRNAs has an important role in tumorigenesis. (biomedcentral.com)
  • Our results indicate that dysregulation of miRNA may play a role in the stem cell-like properties of ovarian CSCs. (biomedcentral.com)
  • Using this method, ovarian CSCs from patients with ascites are first isolated and their ability to exhibit stem cell-like properties is examined [ 6 ]. (biomedcentral.com)
  • In this study, miRNA expression profiles of a CD133 + spheroid-forming subpopulation of OVCAR3 ovarian cancer cells were investigated to identify miRNA expression profiles that contribute toward the characteristics of CSCs in ovarian cancer. (biomedcentral.com)
  • Of the 31 genes, the 21 upregulated genes were primarily associated with cell paracrine and intracellular signaling, transcription regulation and cell adhesion and migration, and their transcriptional products included transforming growth factor-β2 (TGF-β2), insulin-like growth factor-binding protein 2 and transcriptional factor AP-2α/γ ( 11 ). (spandidos-publications.com)
  • Proteome Profiler Mouse Angiogenesis Array Kit (Catalog # ARY015) Desmoglein 3 promotes cancer cell migration and invasion by regulating activator protein 1 and protein kinase C-dependent-Ezrin activation. (bnwax.com)
  • The invasion and migration ability of bladder cancer cells were identified by type I collagen invasion assay and wound closure assay. (chksignal.com)
  • Exploration of these coding genes revealed that the entire genitourinary technique could be mapped inside EVs, which may possibly play an emerging part in cell regulation (167). (gardos-channel.com)
  • Native honey (1% w/v pH 7.4, 10(6) cells) showed strong quenching activity against lipophilic cumoxyl and cumoperoxyl radicals, with significant suppression/prevention of cell damage, complete inhibition of cell membrane oxidation, of intracellular ROS production and recovery of intracellular GSH. (researchgate.net)
  • This indicates that there is inflammation in the urinary tract or kidneys and the body is excreting more white blood cells. (chronicutiinfo.com)
  • Red blood cells - the bladder can bleed due to severe inflammation and the constant urination caused by a UTI. (chronicutiinfo.com)
  • Any evidence of these in the urine on a dipstick test indicates bladder inflammation/infection. (chronicutiinfo.com)
  • Upper panel shows a typical zonula occludens of rat intestinal epithelium, prepared using freeze fracture techniques. (cellimagelibrary.org)
  • The urinary EV cargo suggests that cells along the renal epithelium, extending from the glomerular podocytes (249,250) via the proximal tubule, the thick ascending limb of Henle, the distal convoluted tubule along with the collecting duct, are releasing EVs into urine (18,38). (gardos-channel.com)
  • However, p16 immunoreactivity is not specific for HPV-infected epithelium, as immunoreactivity has previously been documented with inflammatory cells, multinucleated giant cells, bacteria and mucus in cervicovaginal specimens [ 4 - 6 ]. (cytojournal.com)
  • Differential behavior of E-cadherin and occludin in their colocalization with ZO-1 during the institution of epithelial apartment polarity. (daubnet.com)
  • Therefore, a few methods have been developed to use these characteristics in order to assay cancer stem cells. (biomedcentral.com)
  • MiRNA-driven pathways are fundamental for the maintenance and proper function of cell stemness in embryonic stem cells. (biomedcentral.com)
  • Dr. Multani has extensive expertise in the field of Human and Mammalian Cytogenetics, and specializes in the evaluation of genetic instability and complex chromosomal rearrangements in cancer cells, telomere dysfunction, characterization of embryonic stem cells, and authentication of cell lines. (mdanderson.org)
  • One technology receiving much attention is optical coherence tomography (OCT), a non-invasive interferometric imaging technique. (optics.org)
  • Transcriptomic profiling of T-cell populations in non-muscle invasive and muscle invasive bladder cancer. (lu.se)
  • The cells may be uniform or markedly pleomorphic, or form GIANT CELLS. (lookformedical.com)
  • Electron micrograph of the junctional complex of intestinal epithelial cells of the rat shows the apical-most zonula occludens (tight junction), the zonula adherens (medium junction) and the macula ad. (cellimagelibrary.org)
  • The nongranulated cells formed follicles containing a small lumen: the cell apices were joined by junctional complexes including z. (shengsci.com)
  • The congress includes a two-day symposium - Medical Laser Applications 2009 - dedicated to the use of optical techniques for diagnosis and treatment of disease. (optics.org)
  • The American Academy of Pediatrics (AAP) criteria for the diagnosis of UTI in children 2-24 months are the presence of pyuria and/or bacteriuria on urinalysis and of at least 50,000 colony-forming units (CFU) per mL of a uropathogen from the quantitative culture of a properly collected urine specimen. (medscape.com)
  • Diagnosis is based on analysis and culture of urine. (msdmanuals.com)
  • Sputum-culture confirmation guides tuberculosis (TB) diagnosis and patient management but has previously been reported to be low in the US-Affiliated Pacific Islands (USAPI). (cdc.gov)
  • It is widely recognized that the accumulation of various harmful genetic alterations in normal cells may induce malignant cancer cells ( 1 ). (spandidos-publications.com)
  • Behavior of oral SCC cells was examined in three-dimensional culture using type I collagen gel. (bvsalud.org)
  • RESULTS: SCC cells with high integrin αvß8 expression levels had a high ability to migrate on type I collagen and exhibited enhanced invasion into type I collagen gel. (bvsalud.org)
  • In SCC cells with high integrin αvß8 expression level, cultivation on type I collagen induced RAC1 activation. (bvsalud.org)
  • Treatment with RAC1 inhibitor reduced type I collagen-induced motility of SCC cells. (bvsalud.org)
  • Down-regulation of integrin ß8 by specific antisense oligonucleotide reduced type I collagen-induced RAC1 activation and suppressed cell motility and invasion into type I collagen gel. (bvsalud.org)
  • CONCLUSION: The interaction of integrin αvß8 with type I collagen facilitates SCC cell motility and invasion via RAC1 activation. (bvsalud.org)
  • Despite recent achievements of microfluidic 2D cell culture systems [9], they still do not address the fact that in vivo cells coexist in 3D communities that are influenced by spatial orientation of cells and cell-to-cell contact within the extracellular matrix [10]. (heraldopenaccess.us)
  • 2010) and IL-8 production of rhinovirus infected cells was also caused by oxidative stress (Biagioli et al. (biotechpatents.org)
  • I show pictures using each technique, discuss some of their pros and cons and describe how DIC microscopy works. (canadiannaturephotographer.com)
  • The main benefit of microfluidic systems for cell culture analysis is that they can perform a number of crucial liquid handling steps including cell loading, nutrient supply and waste removal under physiologically relevant shear force conditions, all while offering real time microscopy [4]. (heraldopenaccess.us)
  • However, traditional methods to detectTrichomonasincluding culture and wet-mount microscopy, as well as molecular studies, may not always be readily available, particularly on fixed samples received in liquid-based vials for cytologic evaluation. (cytojournal.com)
  • Designed for drug discovery and biomedical research it allows easy labelling, washing and dosing of cells. (valelifesciences.com)
  • spread of metastatic cells [3]. (researchgate.net)
  • or DNA binding -1 (Id-1) has been shown to be increased in several types of advanced cancer, and to be associated with aggressive and metastatic abilities of cancer cells. (chksignal.com)
  • Therefore, we determined the miRNA expression profile of a CD133 + spheroid-forming subpopulation of the OVCAR3 human ovarian cancer cell line. (biomedcentral.com)
  • Lyons VG, Payne NL, McCoy CE (2018) Optimization techniques for miRNA expression in low frequency immune cell populations. (hudson.org.au)
  • Several molecular platforms have been used to investigate the molecular profile of the disease, from high throughput gene expression analyses down to single cell biological dissection. (oaepublish.com)
  • Molecular products metabolized and secreted by neoplastic tissue and characterized biochemically in cells or body fluids. (lookformedical.com)
  • In order to differentiate parasites from degenerated urothelial cells in our case we prepared additional ThinPrep[TM] slides from the residual specimen to perform immunocytochemistry for cytokeratin (using a cocktail of high- and low-molecular weight keratins) and p16 (using primary purified mouse anti-human p16 antibody, clone G175-405, supplied by BD Biosciences Pharmingen, San Diego, CA, USA). (cytojournal.com)
  • Although enterocytes of the small intestine are the primary site of infection by Enterocytozoon bieneusi, it may also spread to other epithelial cells in the biliary tract and nasopharynx causing cholangitis and rhinosinusitis. (powershow.com)
  • If there's no infection or if the vet has reason to suspect bladder stone or other condition other than infection, he may request for a radiograph or an ultrasound. (petinsurancequotes.com)
  • The major defense against urinary tract infection (UTI) is complete emptying of the bladder during urination. (msdmanuals.com)
  • However, urinalysis can help in identifying febrile children who should receive antibacterial treatment while culture results from a properly collected urine specimen are pending. (medscape.com)
  • Negative culture results occur for various reasons, including insufficient sample volume, initiation of antibiotics prior to obtaining an expressed prostatic specimen (EPS) sample, and the presence of fastidious organisms. (medscape.com)
  • And yet even with this advance, in addition to diffusible soluble factor-mediated paracrine and endocrine cell communication as well as EVs, additional niches of intratumoral communication are filled by other modes of intercellular transfer. (frontiersin.org)
  • Normal Rat Kidney (NRK) cells grown in culture expressing Galactosyl Transferase-YFP (GalT-YFP) and p58-CFP. (cellimagelibrary.org)
  • This Antibody was verified by Cell treatment to ensure that the antibody binds to the antigen stated. (thermofisher.com)
  • It showed CTCs grown in 3D (Happy Cell ASM®) retained their proliferative capacity for several weeks post isolation 2D Vs 3D: Cells grown in 2D remained proliferative 35-50 Days compared to those grown in 3D (Happy Cell ASM®) which remained proliferative for approximately 2 weeks longer. (valelifesciences.com)
  • According to James Mechan, Isolation of BTV has traditionally relied on inoculation of cell cultures, embryonated chicken eggs, or sheep with blood from infected animals or with homogenates of insects collected in endemic areas. (purdue.edu)