• Diagram describing the respective places of culture-, poly- merase chain reaction-, serology- and histology-based approaches for culture of the Whipple bacillus, support the effectiveness of the diagnosis of acute bacterial infections, according to the natural this technique (2). (cdc.gov)
  • Colors and staining are often utilized in the field of histology (microscopic examination of living tissues) as well as in areas of medical histopathology, Hematology, and Cytopathology which are focused on studying and diagnosis of disease at a microscopic scale. (microbiologynote.com)
  • Heavy metal staining of paraffin epoxy and glycol methacrylate embedded biological tissue for scanning electron microscope histology. (cdc.gov)
  • Haematoxylin and eosin are among the most commonly used stains in histology. (fact-archive.com)
  • Luna, Lee G. Manual of Histologic Staining Methods of the Armed Forces Institute of Pathology . (newcomersupply.com)
  • 1,2 Similar in structure to silver nitrate, plus fluoride, 1 SDF's biochemical interaction with tooth structure and cariogenic bacteria is also similar (Figure 1). (dimensionsofdentalhygiene.com)
  • In the oral environment, silver diamine fluoride behaves similarly to silver nitrate, plus fluoride. (dimensionsofdentalhygiene.com)
  • Can anyone provide me a supressive procedure in staining degenerating axons using 'common chemicals', just ammonia water, silver nitrate, citric acid, formalin/PFA, sodium hydroxide? (utsouthwestern.edu)
  • This is fundamental to all techniques involving silver nitrate and ammonia, and the exact information is not in chemistry textbooks. (utsouthwestern.edu)
  • To evaluate morphometrically the AgNORs in the automatic image analyzer (AIA) other series of these samples were stained with silver nitrate. (udea.edu.co)
  • Aim: This study evaluated the surface roughness, topography and permeability of bovine enamel by profilometry and scanning electron microscopy (SEM) with and without silver nitrate solution, after exposure to different bleaching agents. (bvsalud.org)
  • For permeability evaluation, the samples were immersed in a 50% silver nitrate solution and analyzed using a backscattered electron and secondary electron mode. (bvsalud.org)
  • The SEM micrographs indicated changes on enamel topography and different levels of silver nitrate penetration in the samples of the bleached groups. (bvsalud.org)
  • In the overall analysis, the bleaching agents promoted surface changes and higher silver nitrate penetration when compared to the control group. (bvsalud.org)
  • Moreover, the higher infiltration of silver nitrate suggests an easier penetration path for the oxygen molecules into the dentin substrate. (bvsalud.org)
  • enamel, dentin, silver nitrate. (bvsalud.org)
  • A model was proposed to assess the penetration through dental hard tissues, including enamel, using silver nitrate 9 . (bvsalud.org)
  • Although several studies have been focused on morphological changes on dental surfaces after bleaching, this seems to be one of first studies reporting on the jump-start approach using silver nitrate penetration to track and evaluate the possible channels of O- penetration. (bvsalud.org)
  • Learn enrobing, pouring stained-glass sugar ornaments, candied fruits, and gingerbread figurines that are instant keepsakes. (surlatable.com)
  • Recently, antibodies are used to stain specific proteins: this is called immunohistochemistry. (fact-archive.com)
  • The slides were stained with hematoxylin and eosin, silver colloid method for NORs and immunohistochemistry for the hormone expressions. (tubitak.gov.tr)
  • These cutting-edge techniques address such important tasks as sample preparation, 2D-PAGE, gel staining, mass spectrometry, and post-translational modification. (nhbs.com)
  • Utilizing the same chemistry as black and white photography, silver staining is another highly sensitive method for the visualization of protein bands on electrophoresis gels. (nationaldiagnostics.com)
  • The staining process requires the use of a mordant , so that the stain is able to adhere with layers on the flagella permitting visualization. (microbiologynote.com)
  • Cajal's precise understanding of anatomy is echoed in modern visualization techniques. (discovermagazine.com)
  • Gram stain has also proven useful to routinely diagnose H. others in the sequence of events leading to isolation of a new pylori and H. heilmanii in the gastric mucosa of patients with microorganism? (cdc.gov)
  • Flagella are too small to be seen with a bright-field microscope using normal stains, like the Gram stain or a simple stain. (microbiologynote.com)
  • Gram stain is an significant technique of differential staining that is used to establish the initial characterisation and classification of microbiology-related bacteria. (microbiologynote.com)
  • Gram staining is a method to detect bacteria pathogens in the specimens and in cultures through the Gram reactions (Gram-positive as well as Gram-negative) and the morphology (cocci/rod). (microbiologynote.com)
  • In Gram staining, Gram positive bacteria appear purple and Grams negative is pink in the color. (microbiologynote.com)
  • from other non-acid fast bacteria which are not stained with Gram stainin. (microbiologynote.com)
  • Biological staining is also employed to distinguish cells during flow cytometry and also to identify the presence of nucleic acids or proteins in electrophoresis gel. (microbiologynote.com)
  • The electrophoresis gel stains are reagents used in lab assays to visualize the separated protein bands or nucleic acids in gels. (excedr.com)
  • Bielschowsky silver staining of the cortex at 400× magnification demonstrates a neurofibrillary tangle (black arrow) and a neuritic plaque (white arrow). (medscape.com)
  • Both amyloid plaques and neurofibrillary tangles are readily identified using silver staining techniques such as Bielschowsky or Gallyas. (medscape.com)
  • All laboratory-based protocols follow the successful Methods in Molecular BiologyT series format, each offering step-by-step laboratory instructions, an introduction outlining the principle behind the technique, lists of the necessary equipment and reagents, and tips on troubleshooting and avoiding known pitfalls. (nhbs.com)
  • Reagents for this procedure are sold as individual stain solutions and are available for purchase under separate part numbers with storage requirements and expiration date designated per bottle. (newcomersupply.com)
  • The goal of staining with simple methods is to clarify the morphology and arrangement the bacterial cells. (microbiologynote.com)
  • In this case, two staining methods are employed to impart distinct hues to various bacteria or structures of bacterial, that aid in distinguishing bacteria. (microbiologynote.com)
  • In the 1950s, major advances were made in the development of methods to trace neural connections using silver staining of degenerating axons. (scholarpedia.org)
  • In the 1970s, these methods were replaced by more powerful techniques based on anterograde and retrograde transport of tracers, enabling neuroethologists to study pathways in the central nervous system involved in neural control of specific behavioral patterns. (scholarpedia.org)
  • In the future, the Cheng Lab will continue to develop new staining and optical methods for expanding the applications of histotomography. (sciencedaily.com)
  • One of these methods is the use of silver stained nucleolar organizer region (Ag +- NOR). (iium.edu.my)
  • Many of the principles explained in Part 2 of this paper apply also to other silver methods. (utsouthwestern.edu)
  • Made by Reusche for traditional stained glass techniques. (creativeglassguild.co.uk)
  • I have included a piece based on a medieval tile design which uses silver stained glass with Reusche pigment painted on as a wash, then the design removed before firing. (scottishglasssociety.com)
  • The samples were embedded in paraffin and cutted to four microns and stained with Hematoxylin-Eosin to check the diagnostic and Giemsa stained to classify the mast cells tumors. (udea.edu.co)
  • Amyloid angiopathy can be identified using stains for amyloidal protein (Congo red, thioflavin-S), or immunohistochemical staining against Aβ (see the image below). (medscape.com)
  • Bacterial structures and cells that are too small to be observed under a light microscope, are thickened due to the impregnation with silver salts on their surface, making them apparent, e.g., for demonstration of flagella of bacterial species and Spirochetes. (microbiologynote.com)
  • Modest increases in reticulin fibers can be difficult to appreciate but can be confirmed with a silver impregnation technique, such as Gomori stain. (nih.gov)
  • Nauta WJH, Gygax PA (1951) Silver impregnation of degenerating axon terminals in the central nervous system: (1) Technic. (utsouthwestern.edu)
  • Staining appears to be dependent upon the initial reduction and resulting immobilization of a small number of silver ions by the proteins in the gel. (nationaldiagnostics.com)
  • After cell wall disruption, the cell wall and cytoplasmic proteins were submitted to PAGE and SDS-PAGE techniques. (scialert.net)
  • 2005). Different types of electrophoretic techniques have been used for the characterization or typing of C. albicans isolates including separation of chromosomes, DNA fragments, isoenzymes, cell wall glycoproteins and whole cell proteins (Asakura et al . (scialert.net)
  • Note that both help strengthen the crystalline structure of the tooth by forming fluorapatite, and both use silver to interact with the thiol groups, causing damage to the deoxyribonucleic acid and proteins in bacteria. (dimensionsofdentalhygiene.com)
  • Gel electrophoresis is a commonly used technique in molecular biology and life sciences labs to separate proteins and nucleic acids (both DNA and RNA) based on their molecular size. (excedr.com)
  • Coomassie blue stain non-specifically binds to proteins, and, when the excess stain is removed by destaining, you can easily observe blue protein bands. (excedr.com)
  • Flagella stain Red while Vegetative cells have been stained Blue. (microbiologynote.com)
  • This became possible after Walter Rudolf Hess (1881-1973) developed in the late 1920s the focal brain stimulation technique to examine how regions within the diencephalon control vegetative functions in cats. (scholarpedia.org)
  • In 1906, Cajal and Camillo Golgi (the Italian physician-scientist who developed the tissue staining technique that Cajal used) shared the Nobel Prize in Physiology or Medicine "in recognition of their work on the structure of the nervous system. (nih.gov)
  • The science of tissue staining is called histochemistry. (fact-archive.com)
  • http://www.informaworld.com/smpp/title~content=t713692932~bb=all The journal's name shows on the present publisher's web site in its present form (Biotechnic & Histochemistry) even though the name was really Stain Technology before about 1990. (utsouthwestern.edu)
  • All Newcomer Supply stain procedures are designed to be used with Coplin jars filled to 40 ml following the provided staining procedure. (newcomersupply.com)
  • Congo red staining of a small cortical artery at 400× magnification demonstrates salmon-colored amyloid deposition in the media of the vessel. (medscape.com)
  • 05). However, in recurrent patients, nucleolar silver staining was more frequently detected as clusters of AgNORs. (tubitak.gov.tr)
  • El presente estudio tuvo como objetivo caracterizar las AgNORs en algunos tipos de tumores cutáneos caninos, para lo cual se evaluaron 28 mastocitomas, 18 carcinomas espinocelulares y siete epiteliomas basocelulares, procedentes del archivo de Patología Animal y del Consultorio Veterinario de la Universidad de Antioquia, y de otros consultorios de la ciudad de Medellín, Colombia. (udea.edu.co)
  • Se evaluaron los parámetros: área nuclear, área AgNORs/célula, número de AgNORs/ célula, y distribución AgNORs en la célula. (udea.edu.co)
  • La coloración AgNORs fue factible en los tumores evaluados para lo cual se necesitaron de 30 minutos en el período de incubación. (udea.edu.co)
  • 0.05) en el número y en el área de las AgNORs entre los tres grados histopatológicos de este tumor. (udea.edu.co)
  • La coloración de AgNORs reveló dos poblaciones celulares en los mastocitomas, que difieren con la clasificación gradativa utilizada en la actualidad. (udea.edu.co)
  • A silver stain technique is used to identify the Nucleolar Organizer Regions (AgNORs). (udea.edu.co)
  • The AgNORs stain is viable in the evaluated tumors for what 30 minutes were needed for the incubation period. (udea.edu.co)
  • The stained AgNORs revealed two cellular layers in the mast cell tumors that differ from the grade classification that is used today. (udea.edu.co)
  • Stains are used to identify biological tissues (highlighting the, for instance muscles fibers or connective tissue) Cell populations (classifying various blood cells) or organelles within each cell. (microbiologynote.com)
  • Collagen fibers are identified as eosinophilic fibers that may be in bundles and are confirmed with the use of a trichrome stain (e.g. (nih.gov)
  • Other compound used to color tissue sections include saffron , silver salts and numerous artificial dyes originally developed to stain cloth fibers. (fact-archive.com)
  • Epoxy embedded tissue was postfixed in Os04, mounted on carbon-coated nylon grids and stained with uranyl acetate and lead citrate. (cdc.gov)
  • BGMA sections were stained with uranyl acetate, lead citrate, silver proteinate and phosphotungstic acid. (cdc.gov)
  • A histolgical artifact is a structure or feature of the sample that is absent in living tissues, but introduced during preparation or staining (1). (fact-archive.com)
  • Silver diamine fluoride (SDF) has been used internationally for years to treat dentinal hypersensitivity and caries lesions. (dimensionsofdentalhygiene.com)
  • One consideration when deciding whether SDF is appropriate is the agent's unesthetic properties, as it will permanently stain caries lesions black (Figure 2). (dimensionsofdentalhygiene.com)
  • This agent should not be used on patients with a silver allergy, 1 and its use should be avoided in patients with open lesions, stomatitis, or ulcerative gingivitis. (dimensionsofdentalhygiene.com)
  • Given its propensity to stain lesions black, one of the most important steps prior to the use of SDF is to obtain informed, written consent from the patient and/or parent/caregiver. (dimensionsofdentalhygiene.com)
  • Basic stains that contain positive charged chromogens are preferred since the nucleic acids of bacterial cells and cell wall components possess negative charges that strongly attracted and bonds to the anionic colorant. (microbiologynote.com)
  • Wet mount techniques are employed to stain flagella of bacterial species It is easy and beneficial when the quantity and configuration of flagella is essential to identify the species of motile bacteria. (microbiologynote.com)
  • Phenotypical and molecular techniques were used for bacterial isolation, characterization and classification of enterotoxigenic E. coli (ETEC) pathotypes. (scielo.br)
  • I would like to receive any proved protocol of silver staining for degenerating axons. (utsouthwestern.edu)
  • Part 2 was an appendix to their paper on the early (non-suppressive) Nauta-Gygax method, which stains both normal and degenerating axons. (utsouthwestern.edu)
  • This means that counts of Ag-NOR could be of great diagnostic value for the cytopathologists in conjunction with routine pap stain to evaluate cellular proliferative activity of the lesion and for follow up of patients after treatment to detect regression in the severity of the lesion. (iium.edu.my)
  • Many individuals think that there is only problem when the stain can be seen, but what happens when you cannot detect the stain? (carpetcleanerssilverspring.com)
  • The silver stain is a more sensitive colorimetric method than coomassie staining as it can detect protein even present in a lower quantity. (excedr.com)
  • Staining is a method used to boost the contrast of samples, usually at the microscopic scale. (microbiologynote.com)
  • Plastic ( Part 5500 ), plastic-tipped, or paraffin coated metal forceps must be used with any silver solution to prevent precipitation of silver salts. (newcomersupply.com)
  • Researchers have developed a new technique that images every pigment cell of a whole zebrafish in 3D. (eurekalert.org)
  • Researchers have developed a new technique that allows scientists to visualize every cell containing melanin pigment in 3D, in whole zebrafish. (sciencedaily.com)
  • Spencer Katz, an MD/PhD medical scientist training program student on Cheng's team, has modified this micro-CT technique to specifically investigate melanin, a pigment scientists are studying in human skin color and melanoma research, in whole zebrafish. (sciencedaily.com)
  • Remove control slide, rinse in warm distilled water, check microscopically for adequate silver development. (newcomersupply.com)
  • In this method the smear of bacteria is stained using a single reagent, which creates distinct appearance of the organism against the background. (microbiologynote.com)
  • Paraffin sections were stained with silver. (cdc.gov)
  • Paraffin and BGMA sections were histologically stained for LM. (cdc.gov)
  • Microscopy is a technique that uses microscopes to examine very small objects, not seen by the naked eye. (loinc.org)
  • This paper describes three techniques for preparing biological tissue sections for correlation of light microscopy (LM) and scanning (SEM) and transmission (TEM) electron microscopy. (cdc.gov)
  • Other advanced techniques include in situ hybridization to identify specific DNA or RNA molecules, and confocal microscopy. (fact-archive.com)
  • The silver granules which make up the latent image act to catalyze this process, resulting in more rapid deposition of silver at the sites of the protein bands. (nationaldiagnostics.com)
  • The stain involves the use of two forms of dyes: G-250 ("colloidal") or the R-250 form. (excedr.com)
  • Light microscopes are utilized to examine stained samples with high magnification. (microbiologynote.com)
  • At Pilchuck, he was introduced to the schwarzlot (transparent black enamel and silver stain) painting technique by the artist Ursula Huth (German, b. 1952). (cmog.org)
  • golden armbands decorated with the motifs made by inlaid enamel obtained from pulverized glass set in design fields and frited: "cloisonné" technique (two examples in the Museum of Byzantine Culture). (egnet.net)
  • Dip a cloth or sponge in the warm, sudsy water and wipe the stain. (howstuffworks.com)
  • If safe to use, rub the stain with a cloth dampened with a few drops of alcohol. (howstuffworks.com)
  • This technique showed high specificity in addition to the significant data for classification (Rodrigues et al . (scialert.net)
  • Ethidium bromide (EtBr) is a commonly used DNA stain, which is used while pre-casting or post-staining. (excedr.com)
  • The two most commonly used protein stains include Coomassie Brilliant Blue and Silver stains. (excedr.com)
  • To see the tissue under a microscope , the sections are stained with one or more pigments. (fact-archive.com)
  • In addition to aiding in dis- covering new pathogens, these techniques also contributed to studies of the epidemiology, pathophysiology, and treatment response of the newly recognized diseases, providing further evidence for causal relationships between disease and organ- ism (1). (cdc.gov)
  • Because of the spectacular molecular techniques developed in the last decades, some authors think that these techniques will shortly supplant culture. (cdc.gov)
  • The Tree of Jesse window is on the right Detail of the Tree of Jesse window, Baslica of Saint-Denis (1140-44) Only a very small amount of original 12th-century French stained glass remains. (wikipedia.org)
  • Most advances have evolved in a series of small steps based on several techniques that have been used successively by different investigators who faced clinically suspect diseases. (cdc.gov)
  • Experimenting with different types of wax, you will carve, cast, and finish at least one small fetish, pendant, or ring in silver. (thecrucible.org)
  • Large BGMA or many small epoxy sections can be stained and examined on a single planchet. (cdc.gov)
  • I have also used mosaic technique, gluing glass pieces to create different heights as I have done with the green leaf against the sky. (scottishglasssociety.com)
  • Just when you thought it was safe to go back into the water you find out that even it can stain your laundry and household surfaces. (howstuffworks.com)
  • One of the most common mistakes a person can make is throwing stained sheets in the laundry, even though this will get rid of some of the traces, it is not guaranteed to rid your fabric of the stain completely and do you really wanna take a risk on your health? (carpetcleanerssilverspring.com)
  • The purpose of negative staining is to investigate the morphological structure the size and arrangement of bacteria cells that are difficult to color. (microbiologynote.com)
  • Regardless of your cleaning or maintenance needs, we at Silver Olas are truly dedicated to providing superior results and an overall unsurpassed level of customer service. (silverolas.com)
  • Cutting-Edge Technology At Silver Olas, we invest in the latest cleaning technologies to provide you with unmatched results. (silverolas.com)
  • Schedule your cleaning appointment with Silver Olas today! (silverolas.com)
  • Silver Olas defines carpet cleaning at its finest. (silverolas.com)
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  • If you haven't found out by now, we are talking about us Carpet Cleaners Silver Spring the best cleaning company in all of Silver Spring to service your pet stain & odor problem for you! (carpetcleanerssilverspring.com)
  • French Gothic stained glass windows were an important feature of French Gothic architecture, particularly cathedrals and churches built between the 12th century and 16th century. (wikipedia.org)
  • The Abbot Suger, a close advisor of the French King, who directed the government during the King's absence during the Crusades, rebuilt the ambulatory with Gothic rib vaults primarily to create greater space for stained glass windows. (wikipedia.org)
  • This continued to be the guiding reason for stained glass windows throughout the Gothic period. (wikipedia.org)
  • Pierre de Roissy, a dignitary of the Chapter of Chartres Cathedral, wrote in about 1200 that the stained glass windows being installed in the nave "would transmit the clarity of the sunlight, signifying the Holy Scriptures, which push evil away from us, as they illuminate us. (wikipedia.org)
  • Stained glass windows have been an integral part of religious architecture for centuries. (h2g2.com)
  • Since stained glass windows were largely to be found in churches, the designs usually had a religious theme, and in some cases could portray complex Biblical scenes in an almost comic-book format. (h2g2.com)
  • The church's 28 stained glass windows are the memorial gifts of more than a dozen extended families plus the church's Senior Friendship Group. (bethesdalutheranchurch.com)
  • I continue to try new subjects and techniques, and most of my interest and influence comes from Scottish historical sites, such as the Iona carved stone and the St Magnus medieval floor tile design I have submitted. (scottishglasssociety.com)
  • For Suger, stained glass became a way to create a glowing, unworldly light ideal for religious reflection. (wikipedia.org)
  • The NOR-related protein becomes visible in nucleus by a silver-staining technique under a light microscope, and it has been named argyrophilic protein of NOR (Ag-NOR). (karger.com)
  • Counterstain in Light Green SF Yellowish Stain 0.2%, Aqueous ( Part 12202 ) for 1-2 minutes. (newcomersupply.com)
  • Nucleic acid stains are chemicals that insert themselves into the structure of the nucleic acids and fluoresce with UV light excitation. (excedr.com)
  • Sufficient silver deposition is visible as a dark brown or black band on the gel. (nationaldiagnostics.com)
  • C: Note the black staining at the gingiva around the recurrent caries in this image taken a month after application. (dimensionsofdentalhygiene.com)
  • In his thirties, he learned about a new way to stain tissue developed by the Italian Camillo Golgi: a new silver-based stain that turned some nerve cells completely black while leaving most others entirely untouched, rendering the delicate branches of the nerve processes clearly visible (as seen in the image on the left). (discovermagazine.com)
  • Staining does not have to be restricted to biological materials. (microbiologynote.com)
  • Many of the concepts and techniques of neuroethology are derived from other biological disciplines, including ethology, neurophysiology, neuroanatomy , neuroendocrinology, and biological cybernetics. (scholarpedia.org)
  • The amount of reticulin staining in the bone marrow often has no correlation with disease severity, while increases in collagen staining are associated with more severe disease and a poorer prognosis. (nih.gov)
  • In this article, we will cover more on the types of gel electrophoresis stains, including their types, applications, and industries frequently using these chemicals in their research or investigation processes. (excedr.com)
  • What are the Types of Gel Electrophoresis Stains? (excedr.com)
  • Many types of gel electrophoresis stains are available today in the market. (excedr.com)
  • The AgNOR technique, which reveals these regions, has rarely been used in diagnosis and preceding the prognosis of pituitary adenoma. (tubitak.gov.tr)
  • We have decided to create this informative area of our website to give our perspective on common techniques used to make stained glass. (downeaststainedglass.com)
  • I only have the improved method by Fink and Heimer in 1967 published on brain research, giving out two staining procedures. (utsouthwestern.edu)
  • We will give descriptions of procedures as well as detailed explanation of techniques used for each phase of stained glass work. (downeaststainedglass.com)
  • There are also some fluorescent dye stains for the total protein that are used for their fast and easy procedures. (excedr.com)
  • Includes step-by-step techniques, procedures, and practical overviews. (lu.se)
  • Bleaching treatment for vital teeth is a conservative technique obtaining suitable results when compared to more invasive procedures used in aesthetic and cosmetic rehabilitation 2 . (bvsalud.org)