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  • membrane
  • McNeil, Paul L. 1991-07-01 00:00:00 10.1002/jcp.1041480102.abs Cells of gut and skin frequently suffer mechanically‐induced plasma membrane disruptions in vivo, and bioactive molecules, including basic fibroblast growth factor (bFGF), could enter and leave cytoplasm through these disruptions. (deepdyve.com)
  • We here provide three lines of evidence that bFGF is released with surprising efficiency through plasma membrane disruptions, resembling those known to occur in vivo, produced by scraping endothelial cells from their culturing substratum. (deepdyve.com)
  • Last, cells that survived membrane disruptions released, and contained, less bFGF on each subsequent wounding, consistent with release of bFGF through transient (i.e., survivable) membrane disruptions. (deepdyve.com)
  • A polyclonal antibody against bFGF completely neutralized the growth promoting activity released by scraping, confirming that bFGF is released through endothelial cell plasma membrane disruptions. (deepdyve.com)
  • We conclude that many characteristics of bFGF-its broad spectrum of producing and target cell types, cytosolic location, efficient release through biologically and pathologically relevant plasma membrane wounds, and its release from cells that survive membrane wounds-make it a strong candidate as a "wound hormone" for rapidly initiating the cell growth required for routine maintenance of tissue integrity and/or repair after injury. (deepdyve.com)
  • The cell membrane is broken through different mechanisms. (emailwire.com)
  • These malarial enzymes digest proteins of the cytoplas-matic membrane of the red blood cells thereby affording invasion and infection by the parasite performing several functions such as mediation of the merozoite entry into host erythrocytes (Braun-Breton et al. (scielo.br)
  • Trypanosoma cruzi oligopeptidase B mediates intracellular [Ca 2+ ] i -transients in mammalian cells promoting actin rearrangements and lysosome-plasma membrane fusion, which is required for efficient parasite invasion (Burleigh & Woolsey 2002). (scielo.br)
  • bounded by nuclear envelope Genetic material within nucleus Contains cytoplasm with cytosol and membrane-bound organelles Cytoplasm = Entire region between the nucleus and cell membrane Cytosol = Semi-fluid medium found in the cytoplasm Geometric relationships explain why most cells are microscopic Cell Type Mycoplasmas Most bacteria Most eukaryotic cells Diameter 0.1 - 1.0 µM 1.0 - 10.0 µm 10.0 - 100.0 µm Range of cell size is limited by metabolic requirements. (studyres.com)
  • The surface area of the plasma membrane must be large enough for the cell volume to provide an adequate exchange surface for oxygen, nutrients and wastes. (studyres.com)
  • The plasma membrane Internal membranes compartmentalize the functions of a eukaryotic cell The average eukaryotic cell has a thousand times the volume of the average prokaryotic cell, but only a hundred times the surface area. (studyres.com)
  • Eukaryotic cells compensate for the small surface area to volume ratio by having internal membrane which: Partition the cell into compartments. (studyres.com)
  • Enclosed by a nuclear envelope a double membrane which encloses the nucleus in a eukaryotic cell. (studyres.com)
  • Microtubules are major components of cilia and flagella, which are tail like projections that are covered by a plasma membrane and extend outwards from the cell. (modernbio.com)
  • Ultracentrifuges can also be used in the study of membrane fractionation. (wikipedia.org)
  • tumor
  • Researchers may order cells from the tumor catalog, acquire microarrays of sections of formalin-fixed, paraffin-embedded clinical tumor samples, or obtain samples from various NIH-sponsored repositories of chemical and biological substances. (sciencemag.org)
  • Radiation therapy is commonly applied to the cancerous tumor because of its ability to control cell growth. (wikipedia.org)
  • Assays
  • On the other hand, older generation qualitative assays, especially bioassays, may be much more gross and less quantitative (e.g., counting death or dysfunction of an organism or cells in a population, or some descriptive change in some body part of a group of animals). (wikipedia.org)
  • laboratory
  • This course will give students hands-on experience with the techniques used in the biochemistry laboratory. (laurentian.ca)
  • The program provides a challenging series of seven 3-hour laboratory sessions which are intended to give students hands-on-experience and a detailed understanding of these new investigative techniques and their potentials. (modernbio.com)
  • He is currently a Professor at Cold Spring Harbor Laboratory (CSHL) and head of the Gene Regulation and Cell Proliferation program of the CSHL Cancer Center. (wikipedia.org)
  • biological
  • It is largely due to Novikoff's bold and imaginative use of morphological techniques," de Duve praised him, "that lysosomes have come to be recognized in a broader biological context. (wikipedia.org)
  • Application of magnetic resonance techniques to the study of biological macromolecules. (bucknell.edu)
  • Histology was once empirical The study of histology began with the development of simple microscopes and techniques for preparing thin slices of biological material to make them suitable for examination. (writework.com)
  • Cellulare theory of the structure of living organisms that established the cell as the basic building block of most biological material. (writework.com)
  • The journal was launched in June 2006 and the content includes both classical methods and cutting-edge techniques relevant to the study of biological problems. (wikipedia.org)
  • To discover the RCF that you have been applying at 3000 rpm, the above formula can be simplified to: [Short cut for 3000 rpm, with only a 0.62% error:] RCF (x g) = 10 x Radius (mm) Microcentrifuges are used to process small volumes of biological molecules, cells, or nuclei. (wikipedia.org)
  • morphological
  • Collections of cells having similar morphological characteristis were described as forming tissues and these were subdivided into: epithelial tissues describe cells that cover surfaces, muscular tissues describe cells with contractile properties, nervous tissues describe cells forming the brain, spinal cord and nerves and connective tissue which describes cells that produce extracellular matrix and serv to link or support other specialized tissues by forming tendons, bones or fatty tissue. (writework.com)
  • some recognize specific cell types or structures and are thus useful as morphological markers. (sciencemag.org)
  • compartments
  • Electron microscopic immunolocalization using the same polyclonal antibody showed the enzyme present at the cell surface, as well as in cytoplasmic membranous compartments of the parasite. (scielo.br)
  • microscopic
  • Histology was developed on light microscopic analysis of cells accompanied by a limited understand of cell physiology and function. (writework.com)
  • Dilution follows a microscopic examination of the source culture that predicts that a few of the growing containers contain a single cell of the desired species. (wikipedia.org)
  • spectroscopic
  • In particular many of the basic spectroscopic and spectrometric techniques were discovered in the early 20th century and refined in the late 20th century. (wikipedia.org)
  • blood
  • Many immune cells are also blood cells. (writework.com)
  • It is an extracorporeal blood separation method whereby whole blood is extracted from a donor or patient, the red blood cells are separated, and the remaining blood is returned to circulation. (wikipedia.org)
  • For the separation of erythrocytes, whole blood is passed through an apparatus that isolates the red blood cells from the remaining components. (wikipedia.org)
  • This is because the hematocrit, or the percentage of blood volume taken up by red blood cells, is present in the highest percentage of all blood cell components in the solid portion of blood. (wikipedia.org)
  • The centrifugal force will separate the red blood cells from other cells due to their high specific weight. (wikipedia.org)
  • In addition, blood separation on microdevices containing capillary channels has been identified as a potential blood cell fractionation method. (wikipedia.org)
  • citation needed] Therapeutic erythrocytapheresis is commonly used to remove red blood cells in patients experiencing sickle cell crisis. (wikipedia.org)
  • After removal of the affected cells, the patient can then be transfused with a dose of normal red blood cells. (wikipedia.org)
  • This includes either two standard units of red blood cells or one unit plus of red blood cells and another of either plasma or platelets. (wikipedia.org)
  • Biomanufacturing products are recovered from natural sources, such as blood, or from cultures of microbes, animal cells, or plant cells grown in specialized equipment. (wikipedia.org)
  • Enzyme
  • One benefit of a metabolic pathway compared to a single-enzyme reaction is that a cell can often precisely regulate the amount of product generated by independently controlling the catalytic activity of certain enzymes in the pathway. (sciencecourseware.org)
  • physiology
  • Albert Claude (24 August 1899 - 22 May 1983) was a Belgian medical doctor and cell biologist who shared the Nobel Prize in Physiology or Medicine in 1974 with Christian de Duve and George Emil Palade. (wikipedia.org)
  • Cell metabolism is dependent on the combined actions of many different enzymes that are essential for the anabolic and catabolic reactions that must occur to maintain the physiology of a cell. (sciencecourseware.org)
  • Fluorescence
  • There are two instruments in use based on its principle: a) Flow cytometer b) Fluorescence-activated cell sorter The cells can be separated by blocking metabolic reactions. (wikipedia.org)
  • Tissue
  • A tissue is an assembly of cells that are arranged in a regular formation. (writework.com)
  • Support cells, not connective tissue "Connective tissue" is used historically to describe a wide range of living material containing cells associated with a dominant extracellular matrix component, who the oretical function was to act as a supporting stroma service more highly specialized cell types. (writework.com)
  • metabolic
  • Sequester reactions, so they may occur without interference from incompatible metabolic processes elsewhere in the cell. (studyres.com)
  • Feedback inhibition is another mechanism that cells can use to regulate a metabolic pathway. (sciencecourseware.org)
  • Two types of metabolic blockades are in use: During the S phase of cell cycle, DNA synthesis can be inhibited by using inhibitors such as thymidine, aminopterin, hydroxyurea and cytosine arabinoside. (wikipedia.org)