The protein that coordinates these activities is transcription factor IID (TFIID), which binds to the core promoter to position the polymerase properly, serves as the scaffold for assembly of the remainder of the transcription complex, and acts as a channel for regulatory signals. (antikoerper-online.de)
TFIID is composed of the TATA-binding protein (TBP) and a group of evolutionarily conserved proteins known as TBP-associated factors or TAFs. (antikoerper-online.de)
Gene5
TATA-box binding protein like 2 is a protein that in humans is encoded by the TBPL2 gene. (wikipedia.org)
Gene Ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) enrichment analyses revealed that the candidate reference genes were significantly enriched in cellular metabolic process, protein metabolic process and ribosome in early development and normal adult tissues as well as in cellular localization and endocytosis in the hindgut under sulfide stress. (biomedcentral.com)
The env gene maintained an ORF coding for a 538-amino acid polypeptide that has all the characteristic features of env proteins and mediates intercellular fusion in vitro [ 11 - 13 ]. (ecancer.org)
Thus the inhibitory effects of SLTM on gene expression appear to result from generalized down-regulation of mRNA synthesis and initiation of apoptosis consequent upon overexpressing the protein. (bris.ac.uk)
As in the case of the G box sequence, previous [ribulose-1,5-bisphosphate carboxylase small subunit] RBCS gene expression studies with transgenic plants have failed to demonstrate a requirement for these I box sequences, although mutation of what are likely to be functionally related sequences in CAB genes does affect expression (Gidoni et al. (wikiversity.org)
Promoter6
Initiation of transcription by RNA polymerase I requires the formation of a complex composed of the TATA-binding protein (TBP) and three TBP-associated factors (TAFs) specific for RNA polymerase I. This complex, known as SL1, binds to the core promoter of ribosomal RNA genes to position the polymerase properly and acts as a channel for regulatory signals. (avivasysbio.com)
Experiments "show that site-specific mutations in either G or I, but not GT boxes, in the context of the full rbcS-IA promoter, drastically affect the rbcS-IA promoter-dependent expression of Adh and [ β -glucuronidas] GUS reporter genes. (wikiversity.org)
This lack of transcription could be due to a mutation in the RBCS3A promoter creating the so-called F-box, a protein binding site located between the activating cis elements, the I-box and G-box. (wikiversity.org)
It "appears that the RBCS2 promoter and, by extension, the RBCS1 and RBCS3A promoters, are activated by the co-ordinate binding of an I-box binding factor (IBF) and a G-box binding factor (GBF) in leaves, while the promoters are activated by a GBF alone in fruit. (wikiversity.org)
They are likely to have conserved DNA sequence elements, called transcription factor binding motifs (TFBMs), in their promoter regions. (biomedcentral.com)
The TBPL2 protein is also known as TBP-related factor 3 (TRF3) and TATA binding protein 2 (TBP2). (wikipedia.org)
Subunit2
In addition, this subunit interacts with the transcription factor CREB, which has a glutamine-rich activation domain, and binds to other proteins containing glutamine-rich regions. (antikoerper-online.de)
Aberrant binding to this subunit by proteins with expanded polyglutamine regions has been suggested as one of the pathogenetic mechanisms underlying a group of neurodegenerative disorders referred to as polyglutamine diseases. (antikoerper-online.de)
Mediates1
The core domain, which mediates this binding to DNA, shows 95% identity between TBP and TBPL2. (wikipedia.org)
TBP dimers do not bind DNA, but they must dissociate into monomers before stably binding to the TATA box. (elsevierpure.com)
Dissociation of TBP dimers appears to be relatively slow, and as such has the potential to dictate the kinetics of DNA binding. (elsevierpure.com)
Serine1
In the nucleus, SLTM has a punctate distribution and it does not co-localize with SR (serine/arginine) proteins. (bris.ac.uk)
Genes2
1991). The first group consists of the RBCS1 , RBCS2 and RBCS3A genes, which contain a conserved pair of I-box (5'-GGATGA-GATAAGA-3') and G-box (5'-CACGTG-3') elements in an identical spatial arrangement (Manzara et al. (wikiversity.org)
Reprime la TRANSCRIPCIÓN GENÉTICA de los GENES diana y desempeña una función fundamental en la ODONTOGÉNESIS. (bvsalud.org)
Yeast1
binds specifically to the I-box and activates transcription in yeast and plants. (wikiversity.org)
TBPL2 Polyclonal Antibody detects endogenous levels of TBPL2 protein. (gbiosciences.com)
Eukaryotic1
The TATA binding protein (TBP) is a central component of all eukaryotic transcription machineries. (elsevierpure.com)
Affinity1
By chemical cross-linking, gel filtration chromatography, and protein affinity chromatography, we find that the conserved carboxyl-terminal DNA binding domain of human TBP dimerizes when not bound to DNA. (elsevierpure.com)
Nuclear1
The nuclear membrane protein SUN1 stabilizes endothelial cell-cell junctions far from the nucleus via regulation of microtubule dynamics and Rho GEF-H1 signaling, revealing long-range cellular communication important for vascular development and function. (elifesciences.org)
It inhibits TCF/β-catenin mediated transcription by competing with β-catenin for CBP (CREB binding protein) but not p300 binding with an IC50 of 3 µM. (linkownia.net)
catenin for CBP (CREB binding protein) but not p300 binding with an IC50 of 3 µM [1]. (linkownia.net)
Interactions1
EPI-001 could target the amino terminal domain of the AR and inhibit the protein-protein interactions which are necessary for AR transcriptional activity. (linkownia.net)
Motif1
Although "the P3, P6 substitutions alter the conserved 'GATAAG' I box motif, a 'GATA' motif is present in the introduced EcoRV site. (wikiversity.org)
Regulation1
RNA-protein multiome approach helps to discover that the posttranscriptional regulation of the translational machinery is crucial for the fidelity of cortical development. (elifesciences.org)
Estrogen1
Breast cancer growth is regulated by estrogen, which acts by binding to its estrogen receptor alpha (ERα). (biomedcentral.com)
Homology1
The sequence targeted by substitution P4 overlaps a sequence showing homology to the 'L' box (consensus 5'-AAATTAACCAA), which is conserved in RBCS upstream sequences of both tomato and tobacco (Giuliano et al. (wikiversity.org)
Member1
The protein was independently discovered in three laboratories as a vertebrate-specific family member of TATA-binding protein (TBP). (wikipedia.org)
Expression1
While indicating a crucial role for SLTM in cellular function, these results also emphasize the need for caution when interpreting phenotypic changes associated with manipulation of protein expression levels. (bris.ac.uk)
Widely1
Here, we offer an example of a potential target that has not been widely studied, namely the syncytin protein. (ecancer.org)
Search1
Use OR to search for either of two terms (e.g. heart OR lung ) or quotation marks to search for phrases (e.g. "dna binding" ). (mpg.de)