AnatomyOrganismsChemicals and DrugsAnalytical, Diagnostic and Therapeutic Techniques and EquipmentPhenomena and ProcessesInformation Science
TATA-Box Binding ProteinTATA BoxTranscription Factor TFIIDTATA-Binding Protein Associated FactorsTranscription Factor TFIIATATA Box Binding Protein-Like ProteinsTranscription Factor TFIIBTranscription FactorsDNA-Binding ProteinsTranscription, GeneticPromoter Regions, GeneticTranscription Factors, TFIIRNA Polymerase IIMolecular Sequence DataBase SequenceProtein BindingSaccharomyces cerevisiae ProteinsSaccharomyces cerevisiaeRNA Polymerase IIIBinding SitesGene Expression Regulation, FungalRNA Polymerase IHeLa CellsDNAAmino Acid SequenceTranscriptional ActivationTrans-ActivatorsRepressor ProteinsNuclear ProteinsRecombinant ProteinsProtein Structure, TertiaryPoly(A)-Binding ProteinsMutationCarrier ProteinsTranscription Factor TFIIIBGene Expression RegulationTacrolimus Binding ProteinsMacromolecular SubstancesRecombinant Fusion ProteinsPoly(A)-Binding Protein IInsulin-Like Growth Factor Binding ProteinsRNA-Binding ProteinsRNA, MessengerCloning, MolecularFatty Acid-Binding ProteinsPoly(A)-Binding Protein IICell LinePeriplasmic Binding ProteinsInsulin-Like Growth Factor Binding Protein 3Calcium-Binding ProteinsSequence Homology, Amino AcidTacrolimus Binding Protein 1ATransfectionLatent TGF-beta Binding ProteinsRegulatory Sequences, Nucleic AcidCyclic AMP Response Element-Binding ProteinInsulin-Like Growth Factor Binding Protein 2CCAAT-Enhancer-Binding ProteinsInsulin-Like Growth Factor Binding Protein 1Retinol-Binding ProteinsPlasmidsRestriction MappingCell NucleusSequence AlignmentS100 Calcium Binding Protein GCREB-Binding ProteinEscherichia coliSequence Homology, Nucleic AcidDNA, ComplementaryMolecular WeightSp1 Transcription FactorVitamin D-Binding ProteinPolypyrimidine Tract-Binding ProteinGenes
TFIIDSubunitPromoterGenesTAFsYeastPreinitiation complexPolymerase IITranscription initiation factorKinaseTFIIAInteractionsAffinityEukaryoticTranscriptionalSpecificityUbiquitinModulatesMRNA and proteinComplexRibosomalPromotersEndogenousMinor grooveCREBAminoGenomeFunctionTranslation initiation factorTFIIBSubunitsAntibodyReplication initiationRecognitionNuclearRegulateGenomicChromosomeRegulationDomainSequenceInduces
TFIID16
  • TBP is a subunit of the eukaryotic general transcription factor TFIID. (wikipedia.org)
  • TFIID is the first protein to bind to DNA during the formation of the transcription preinitiation complex of RNA polymerase II (RNA Pol II). (wikipedia.org)
  • Binding of TFIID to the TATA box in the promoter region of the gene initiates the recruitment of other factors required for RNA Pol II to begin transcription. (wikipedia.org)
  • The RNA polymerase II (Pol II) basal transcription factor TFIID is composed of the TATA box-binding protein (TBP) and several TBP-associated factors (TAFs). (elsevierpure.com)
  • In contrast to what is observed for several other promoters lacking a consensus TATA element, the TATA-binding activity of TBP is not required for the functional recruitment of TFIID to the natural TATA-less TdT and β-polymerase promoters. (elsevierpure.com)
  • Moreover, a comparison of TBP and highly purified epitope-tagged TFIID reveals that one or several TAFs function independently of distal regulatory elements to mediate initiator-directed (basal) transcription from the natural TATA-less TdT core promoter in crude nuclear extracts. (elsevierpure.com)
  • Altogether, our results suggest an alternative pathway for TFIID recruitment to initiator-dependent TATA-less class II promoters in which TAF(s) recruit TBP by interacting either directly or indirectly with the initiator region. (elsevierpure.com)
  • The protein that coordinates these activities is transcription factor IID (TFIID), which binds to the core promoter to position the polymerase properly, serves as the scaffold for assembly of the remainder of the transcription complex, and acts as a channel for regulatory signals. (antikoerper-online.de)
  • TFIID is composed of the TATA-binding protein (TBP) and a group of evolutionarily conserved proteins known as TBP-associated factors or TAFs. (antikoerper-online.de)
  • TAF9 is a TATA-binding protein associated factor (TAF) conserved from yeast to humans and shared by two transcription coactivator complexes, TFIID and SAGA. (thebiogrid.org)
  • The transcription initiation TFIID complex is composed of TATA binding protein (TBP) and a number of TBP-associated factors (TAFs). (embl-heidelberg.de)
  • TFIID binds promoter DNA to recruit RNA polymerase II and other basal factors for transcription. (biorxiv.org)
  • Although the TATA-Binding Protein (TBP) subunit of TFIID is necessary and sufficient for in vitro transcription, the TBP-Associated Factor (TAF) subunits recognize downstream promoter elements, act as co-activators, and interact with nucleosomes. (biorxiv.org)
  • Crystal Structure of a Subcomplex of Human Transcription Factor TFIID Formed by TATA Binding Protein-Associated Factors Htaf4 (Htaf(II)135) and Htaf12 (Htaf(II)20). (cathdb.info)
  • Transcription factor TFIID (or TATA-binding. (genexplain.com)
  • 2550146 . RA Hahn S., Buratowski S., Sharp P. A., Guarente L. RT Isolation of the gene encoding the yeast TATA binding protein TFIID: A gene identical to the SPT15 suppressor of Ty element insertions RL Cell 58:1173-1181 (1989). (genexplain.com)
Subunit7
  • TBP associates with a host of factors, including the general transcription factors TFIIA, -B, -D, -E, and -H, to form huge multi-subunit pre-initiation complexes on the core promoter. (wikipedia.org)
  • In addition, this subunit interacts with the transcription factor CREB, which has a glutamine-rich activation domain, and binds to other proteins containing glutamine-rich regions. (antikoerper-online.de)
  • Aberrant binding to this subunit by proteins with expanded polyglutamine regions has been suggested as one of the pathogenetic mechanisms underlying a group of neurodegenerative disorders referred to as polyglutamine diseases. (antikoerper-online.de)
  • 39504) translation initiation factor 2%2C alpha subunit CP001857 CDS Arcpr_0045 complement(39546. (go.jp)
  • The Wilcoxon test and standardized mean difference (SMD) were utilized to evaluate the mRNA expression level of TATA-box binding protein associated factor, RNA polymerase 1 subunit D (TAF1D). (jcancer.org)
  • Folding of proteins with WD-repeats: comparison of six members of the WD-repeat superfamily to the G protein beta subunit. (embl.de)
  • Members of this family include the signal-transducing G protein beta subunit, as well as other proteins that regulate signal transduction, transcription, pre-mRNA splicing, cytoskeletal organization, and vesicular fusion. (embl.de)
Promoter7
  • In the presence of a TATA-less promoter, TBP binds with the help of TBP-associated factors (TAFs). (wikipedia.org)
  • Here we address the mechanisms of functional TBP recruitment to the TATA-less initiator-dependent promoter of the mouse terminal deoxynucleotidyl transferase (TdT) gene. (elsevierpure.com)
  • TAFs may participate in basal transcription, serve as coactivators, function in promoter recognition or modify general transcription factors (GTFs) to facilitate complex assembly and transcription initiation. (antikoerper-online.de)
  • Initiation of transcription by RNA polymerase I requires the formation of a complex composed of the TATA-binding protein (TBP) and three TBP-associated factors (TAFs) specific for RNA polymerase I. This complex, known as SL1, binds to the core promoter of ribosomal RNA genes to position the polymerase properly and acts as a channel for regulatory signals. (avivasysbio.com)
  • Here we show that transcription induces stable TAF binding to downstream promoter DNA, independent of upstream contacts, TBP, or other basal transcription factors. (biorxiv.org)
  • Transcription initiation at a consensus bacterial promoter proceeds via a 'bind-unwind-load-and-lock' mechanism. (academictree.org)
  • Crystal structure of the T(-26) Adenovirus major late promoter TATA box variant bound to wild-type TBP (Arabidopsis thaliana TBP isoform 2). (expasy.org)
Genes8
  • TATA-less promoters of mRNA-encoding genes often contain an initiator element at the transcription start site that is sufficient to direct accurate Pol II transcription. (elsevierpure.com)
  • On the other hand, LTR-retrotransposons, so called mammalian apparent LTR retrotransposons (MaLRs) represent 3.6% of the genome and lack primer binding sites and gag-pol genes ( 1 ). (frontiersin.org)
  • The core transcription machinery of RNA polymerase copies the information found in DNA genes onto messenger RNA molecules that then govern the production of proteins. (sciencedaily.com)
  • Gene Ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) enrichment analyses revealed that the candidate reference genes were significantly enriched in cellular metabolic process, protein metabolic process and ribosome in early development and normal adult tissues as well as in cellular localization and endocytosis in the hindgut under sulfide stress. (biomedcentral.com)
  • Promoters of housekeeping genes contain built-in activating motifs for factors such as GABPA and YY1, which decrease the responsiveness of promoters to distal enhancers. (nature.com)
  • Maf1 directly represses transcription from both RNA pol II- and III-dependent genes, but not RNA pol I genes, making it a unique transcription factor. (bcm.edu)
  • It binds the vitamin D receptor (VDR) in order to control the expression of a wide range of genes as well as interacting with the epigenome to modify chromatin and methylation status. (biomedcentral.com)
  • At the cellular level, the actions of glucocorticoids are mediated by a 94-kd protein, the glucocorticoid receptor (GR). The human (h) GR belongs to the steroid/thyroid/retinoic acid superfamily of nuclear receptors and functions as a ligand-dependent transcription factor that regulates the expression of glucocorticoid-responsive genes positively or negatively. (medscape.com)
TAFs2
  • We propose a new model in which TAFs function as reinitiation factors, accounting for the differential responses of promoters to various transcription factor mutations. (biorxiv.org)
  • This gene encodes a protein that is a component of the PCAF histone acetylase complex and structurally similar to one of the histone-like TAFs, TAF6. (abnova.com)
Yeast1
  • This process requires the involvement of nuclear transport receptors, collectively termed karyopherin-ß (Kap-ß) in yeast, and various regulatory factors. (sinica.edu.tw)
Preinitiation complex3
  • As one of the few proteins in the preinitiation complex that binds DNA in a sequence-specific manner, it helps position RNA polymerase II over the transcription start site of the gene. (wikipedia.org)
  • We show that the natural TATA-less TdT initiator region is sufficient to promote low levels of specific transcription in vitro and to direct the assembly of a stable preinitiation complex. (elsevierpure.com)
  • RNA polymerase II components and Rrn7 form a preinitiation complex on the HomolD box to promote ribosomal protein gene expression in Schizosaccharomyces pombe. (anid.cl)
Polymerase II3
  • However, it is estimated that only 10-20% of human promoters have TATA boxes - the majority of human promoters are TATA-less housekeeping gene promoters - so TBP is probably not the only protein involved in positioning RNA polymerase II. (wikipedia.org)
  • Within SEC two elongation factors, positive transcription elongation factor b (P-TEFb) and elongation factor for RNA polymerase II 2 (ELL2) are key components. (biomedcentral.com)
  • The AF-1 plays an important role in the interaction of the receptor with molecules necessary for the initiation of transcription, such as coactivators, chromatin modulators, and basal transcription factors, including RNA polymerase II, TATA-binding protein (TBP), and a host of TBP-associated proteins (TAFIIs). (medscape.com)
Transcription initiation factor1
  • In our second major area of research, we have defined an unexpected role for the central transcription initiation factor, TATA-binding protein (TBP) in promoting oncogenesis, and extensively identified intriguing and unanticipated properties of growth factor receptors and signaling molecules that regulate its expression. (bcm.edu)
Kinase1
  • For this purpose, WIN 55,212-2 was injected in pregnant wistar rats from gestation day 5 to 20 and a detailed analysis of the levels of the neurotrophin brain-derived neurotrophic factor (BDNF) as well as of the signaling molecules extracellular signal-regulated kinase (ERK)1/2 and alpha-calcium/calmodulin-dependent protein kinase II (alpha-CaMKII) was carried out in adult offspring. (researchgate.net)
TFIIA1
Interactions1
  • EPI-001 could target the amino terminal domain of the AR and inhibit the protein-protein interactions which are necessary for AR transcriptional activity. (linkownia.net)
Affinity1
  • Here, we designed a set of homology-directed repair donor templates that enable efficient tagging of endogenous proteins with affinity tags by transient transfection and selection of genome-edited cells in various human cell lines. (bvsalud.org)
Eukaryotic2
  • The TAF homology (TAFH) or Nervy homology region 1 (NHR1) domain is a domain of 95-100 amino acids present in eukaryotic proteins of the MTG/ETO family and whereof the core ~75-80 residues occur in TAF proteins. (embl-heidelberg.de)
  • DNA replication occurs once and only once per cell cycle mainly regulated by DNA replication initiation factors in eukaryotic cells. (intechopen.com)
Transcriptional1
  • The scientists had found evidence that TRF1 is apparently one of several alternate transcriptional control molecules-called recognition factors-that can replace the most prevalent control element, called TATA-binding protein, or TBP. (sciencedaily.com)
Specificity1
  • The specificity of the proteins is determined by the sequences outside the repeats themselves. (embl.de)
Ubiquitin1
  • Cyclophilin and ubiquitin extension proteins are suitable for nonvirus-infected sweet potato leaves. (bvsalud.org)
Modulates3
  • This region modulates the DNA binding activity of the C-terminus, and modulation of DNA-binding affects the rate of transcription complex formation and initiation of transcription. (wikipedia.org)
  • The transcription factor TATA-box binding protein (TBP) modulates gene expression in nuclei. (sinica.edu.tw)
  • The team led by Dr. Kuo-Chiang Hsia (Institute of Molecular Biology, Academia Sinica) and Dr. Wei-Yi Chen (Institute of Biochemistry and Molecular Biology, College of Life Sciences, National Yang Ming Chiao Tung University) presents a cryo-EM structure of Kap114p, one of the Kap-βs, in complex with TATA box binding protein and reveals a non-canonical function beyond nuclear transport that modulates TBP-dependent transcription. (sinica.edu.tw)
MRNA and protein2
  • TAF1D was upregulated in OS at the mRNA and protein levels and possessed robust discriminatory power. (jcancer.org)
  • Alternative splicing of the primary transcript gives rise to the 2 mRNA and protein isoforms, hGR-alpha and hGR-beta. (medscape.com)
Complex3
  • function:Transcription factor required in complex with TAF3 for the differentiation of myoblasts into myocytes. (gbiosciences.com)
  • The AML1-MTG8 leukemic fusion protein forms a complex with a novel member of the MTG8(ETO/CDR) family, MTGR1. (embl-heidelberg.de)
  • The AML1-CBFbeta transcription factor complex is essential for the definitive hematopoiesis of all lineages and is the most frequent target of chromosomal rearrangements in human leukemia. (embl-heidelberg.de)
Ribosomal1
  • 55699) Ribosomal protein L15e CP001857 CDS Arcpr_0066 complement(55738. (go.jp)
Promoters5
  • In specific cell types or on specific promoters TBP can be replaced by one of these TBP-related factors, some of which interact with the TATA box similarly to TBP. (wikipedia.org)
  • The binding of TBP to these promoters is facilitated by housekeeping gene regulators. (wikipedia.org)
  • Interestingly, transcription initiates within a narrow region at around 30 bp downstream of TATA box on TATA-containing promoters, while transcription start sites of TATA-less promoters are dispersed within a 200 bp region. (wikipedia.org)
  • The TATA-box binding protein (TBP) is required for the initiation of transcription by RNA polymerases I, II and III, from promoters with or without a TATA box. (wikipedia.org)
  • TBP is required for Pol II transcription from TATA-containing and TATA-less promoters. (elsevierpure.com)
Endogenous2
  • TBPL2 Polyclonal Antibody detects endogenous levels of TBPL2 protein. (gbiosciences.com)
  • Combined with technological advancements in single-particle cryogenic electron microscopy, this strategy allows efficient structural studies of endogenous proteins captured in their native cellular environment and during different cellular processes. (bvsalud.org)
Minor groove1
  • The TBP is an unusual protein in that it binds the minor groove using a β sheet. (wikipedia.org)
CREB2
  • It inhibits TCF/β-catenin mediated transcription by competing with β-catenin for CBP (CREB binding protein) but not p300 binding with an IC50 of 3 µM. (linkownia.net)
  • catenin for CBP (CREB binding protein) but not p300 binding with an IC50 of 3 µM [1]. (linkownia.net)
Amino4
  • When TBP binds to a TATA box within the DNA, it distorts the DNA by inserting amino acid side-chains between base pairs, partially unwinding the helix, and doubly kinking it. (wikipedia.org)
  • TBP binds with the negatively charged phosphates in the DNA backbone through positively charged lysine and arginine amino acid residues. (wikipedia.org)
  • We chose proteins that include amino and carboxyl extensions as well as proteins that are made up entirely of WD-repeats. (embl.de)
  • The human GR is a modular protein composed of distinct regions illustrated in panel B in the image below, as follows: (1) The amino-terminal A/B region, also called immunogenic or N-terminal domain (NTD) and (2) the C, D, and E regions, which correspond to the DNA-binding domain, the hinge region, and the ligand-binding domain, respectively. (medscape.com)
Genome1
  • Two lymphoblastoid cell lines stimulated with 1,25-dihydroxyvitamin D were used to generate a ChIP-Seq genome-wide map, which identified 229 genomic regions differentially bound with VDR before and after treatment with active vitamin D [ 13 ]. (biomedcentral.com)
Function5
  • Research has intensified to understand ERV protein function and their role as tumor antigens and targets for cancer (immune) therapy. (frontiersin.org)
  • 20759) Protein of unknown function DUF54 CP001857 CDS Arcpr_0024 20798. (go.jp)
  • 43228) protein of unknown function UPF0153 CP001857 CDS Arcpr_0051 complement(43225. (go.jp)
  • 44065 protein of unknown function UPF0047 CP001857 CDS Arcpr_0053 complement(44048. (go.jp)
  • 45260 protein of unknown function DUF121 CP001857 CDS Arcpr_0055 complement(45235. (go.jp)
Translation initiation factor1
  • 54053) translation initiation factor eIF-1A CP001857 CDS Arcpr_0064 complement(54088. (go.jp)
TFIIB1
Subunits1
Antibody1
  • Antibody Reactive Against Recombinant Protein. (abnova.com)
Replication initiation1
  • 1251 orc1%2Fcdc6 family replication initiation protein CP001857 CDS Arcpr_0002 1251. (go.jp)
Recognition1
  • TATA element recognition by the TATA box-binding protein has been conserved throughout evolution. (expasy.org)
Nuclear1
  • S/MAR (nuclear scaffold/matrix attachment region) Is a DNA sequence pattern that recruits the nuclear protein hnRNP-U/SAF-A a multifunctional scaffold/matrix specific factor. (stackexchange.com)
Regulate1
  • Further exploring the mechanisms that regulate cellular TBP concentrations, and subsequently, the rate of RNA pol I- and III-dependent transcription, we discovered a new protein, Maf1. (bcm.edu)
Genomic1
  • The precise regulations of pre-RC protein levels and assembly are effective ways to prevent reassembly of de novo MCM2-7 onto the replicated origins to re-license and re-replicate the genomic DNA in the subsequent phases of the same cell cycle ( Figure 1) . (intechopen.com)
Chromosome3
  • 32303) chromosome segregation and condensation protein%2C ScpB CP001857 CDS Arcpr_0039 complement(32287. (go.jp)
  • 32898) chromosome segregation and condensation protein ScpA CP001857 CDS Arcpr_0040 complement(32907. (go.jp)
  • 36314) chromosome segregation protein SMC CP001857 CDS Arcpr_0041 complement(36316. (go.jp)
Regulation3
  • We thought that only one set of "general" proteins was involved, and that all the regulation was directed by enhancer-binding proteins that were specific to a particular gene sequence. (sciencedaily.com)
  • WD-repeat proteins are a large family found in all eukaryotes and are implicated in a variety of functions ranging from signal transduction and transcription regulation to cell cycle control and apoptosis. (embl.de)
  • The goal of our research is to delineate the regulation and roles of transcription factors in oncogenic transformation. (bcm.edu)
Domain10
  • 6097 beta-lactamase domain protein CP001857 CDS Arcpr_0005 complement(6094. (go.jp)
  • 23029 TrkA-N domain protein CP001857 CDS Arcpr_0027 23060. (go.jp)
  • 25637 PKD domain containing protein CP001857 CDS Arcpr_0030 complement(25624. (go.jp)
  • 30270 beta-lactamase domain protein CP001857 CDS Arcpr_0037 complement(30271. (go.jp)
  • 38678) Cobalamin-independent synthase MetE domain protein CP001857 CDS Arcpr_0044 complement(38683. (go.jp)
  • 51868 SMC domain protein CP001857 CDS Arcpr_0061 51865. (go.jp)
  • Taxonomic distribution of proteins containing TAFH domain. (embl-heidelberg.de)
  • The complete taxonomic breakdown of all proteins with TAFH domain is also avaliable . (embl-heidelberg.de)
  • Click on the protein counts, or double click on taxonomic names to display all proteins containing TAFH domain in the selected taxonomic class. (embl-heidelberg.de)
  • DNA-binding and trans-activating domain (bipartite) [7] . (genexplain.com)
Sequence2
  • The TATA-binding protein (TBP) is a general transcription factor that binds specifically to a DNA sequence called the TATA box. (wikipedia.org)
  • TBP is involved in DNA melting (double strand separation) by bending the DNA by 80° (the AT-rich sequence to which it binds facilitates easy melting). (wikipedia.org)
Induces1
  • Ectopic expression of AML1-MTG8 in L-G murine myeloid progenitor cells inhibits differentiation to mature neutrophils and induces cell proliferation in response to granulocyte colony-stimulating factor (G-CSF). (embl-heidelberg.de)