• Tartrate-resistant acid phosphatase (TRAP or TRAPase), also called acid phosphatase 5, tartrate resistant (ACP5), is a glycosylated monomeric metalloprotein enzyme expressed in mammals. (wikipedia.org)
  • Tartrate-resistant acid phosphatase (TRAP) is a basic iron-binding protein with high activity towards phosphoproteins, ATP and 4-nitrophenyl phosphatase. (thermofisher.com)
  • The aim of this study was to prove that human umbilical cord mesenchymal stem cell (hUCMSC) therapy conducted according to the mandibular osteoporotic model will increase Osterix (Osx) and bone morphogenetic protein-2 (BMP-2) expression, while reducing tartrate-resistant acid phosphatase (TRAP) expression. (physiciansweekly.com)
  • The ACP5 gene provides instructions for making an enzyme called tartrate-resistant acid phosphatase type 5 (TRAP). (medlineplus.gov)
  • The ACP5 gene mutations that cause SPENCDI typically change single protein building blocks (amino acids) in the TRAP enzyme or result in the production of an abnormally short enzyme. (medlineplus.gov)
  • Tartrate-resistant acid phosphatase (TRAP) and the osteoclast/immune cell dichotomy. (medlineplus.gov)
  • However, tartrate-resistant acid phosphatase (TRAP) activity and the number of osteoclast cells (TRAP-positive multinucleated cells) were not changed by nSP treatment in the presence of receptor activator of nuclear factor κB ligand (RANKL) at doses that did not induce cytotoxicity by silica particles. (springer.com)
  • The purpose of this study was to investigate the activities of the total acid phosphatase (TAP), tartrate-resistant acid phosphatase (TRAP), low molecular weight protein tyrosine phosphatase (LMW-PTP) and alkaline phosphatase (ALP) enzymes, as well as the possible correlation in the serum and in unstimulated whole saliva of children. (bvsalud.org)
  • The treatment response of serum TRACP5b activity, bone alkaline phosphatase (BAP) activity, and concentrations of NH2-terminal telopeptide of type 1 collagen (NTX) in 68 breast cancer patients with bone metastasis were determined. (tmu.edu.tw)
  • Alkaline phosphatase has been clinically available for several years as a marker for bone metabolism. (medscape.com)
  • In adults with normal liver function, approximately 50% of the total alkaline phosphatase activity arises from the liver and 50% from the bone. (medscape.com)
  • The development of immunoassay-based markers with monoclonal antibodies directed to the bone-specific isoform of alkaline phosphatase has improved specificity and sensitivity. (medscape.com)
  • Changes in bone-specific alkaline phosphatase can lag by several weeks. (medscape.com)
  • In comparison with corresponding baseline values, only dried plum significantly decreased serum levels of bone turnover markers including bone-specific alkaline phosphatase and tartrate-resistant acid phosphatase-5b. (nih.gov)
  • The expression and transcription of alkaline phosphatase (ALP) and BSP promote the maturation and mineralization of OB ( 5 ). (frontiersin.org)
  • The ethanol extract of Paeonia lactiflora Pall (Paeoniceae) significantly increased the differentiation of MC3T3-E1 preosteoblastic cells determined by alkaline phosphatase assay. (ijpsonline.com)
  • Alkaline Phosphatase (ALP), an enzyme anchored in the membrane of osteoblast, plays an important role in the mineralization of bone and the expression of ALP is maximized during the osteoblast differentiation[ 4 ]. (ijpsonline.com)
  • The urinalysis and serum calcium phosphate, alkaline phosphatase and parathyroid hormone were within their reference ranges. (pathologyoutlines.com)
  • The following product was used in this experiment: Tartrate Resistant Acid Phosphatase Monoclonal Antibody (5C5E7) from Thermo Fisher Scientific, catalog # MA5-38435, RRID AB_2898349. (thermofisher.com)
  • A blood smear showed 10% hairy cells, and a bone marrow biopsy confirmed a diagnosis of hairy cell leukemia and interstitial infiltration of CD20-positive, monoclonal antibody DBA.44-positive, and tartrate-resistant acid phosphatase-positive cells. (cdc.gov)
  • Halleen JM, Räisänen S, Salo JJ, Hentunen TA, Reddy SV, Roodman DG, Lehenkari PP, Kaija H, Vihko P, Väänänen HK 1999 Intracellular fragmentation of bone resorption products by reactive oxygen species generated by osteoclastic tartrate-resistant acid phosphatase . (oncobone.com)
  • Nature of mononuclear cells positive for acid phosphatase activity in bone marrow of patients with renal osteodystrophy. (bmj.com)
  • Fifty two of 63 patients with renal osteodystrophy had one or more mononuclear cells positive for acid phosphatase in the marrow. (bmj.com)
  • Transcribed from this gene is a 1.5kb mRNA with an open reading frame (ORF) of 969-975 bp encoding a 323-325 amino acid protein. (wikipedia.org)
  • In the rat, the ORF is 981 bp in length and encodes for a 327-amino acid protein. (wikipedia.org)
  • Human Umbilical Cord Mesenchymal Stem Cell-induced Osterix, Bone Morphogenetic Protein-2, and Tartrate-resistant Acid Phosphatase Expression in Osteoporotic Mandibular Bone. (physiciansweekly.com)
  • Osteocalcin is a small protein (49 amino acids) synthesized by mature osteoblasts, odontoblasts, and hypertrophic chondrocytes. (medscape.com)
  • Protein phosphatases reverse the covalent modifications of numerous cellular proteins imposed by the activation of protein kinases and, therefore, play key role in cell signaling metabolism, growth and differentiation. (bvsalud.org)
  • It is differentiated from other mammalian acid phosphatases by its resistance to inhibition by tartrate and by its molecular weight. (wikipedia.org)
  • Existing studies have confirmed that ferroptosis involves the metabolisms of the Fe, lipids, amino acid, each mechanism is mutually independent but interrelated, and they are formed a complex regulatory network. (frontiersin.org)
  • The number of tartrate-resistant acid phosphatase-positive multinuclear cells in the metaphysis of the tibia did not change significantly with treatment. (nih.gov)
  • Biallelic mutations in ACP5, encoding tartrate-resistant acid phosphatase (TRACP), have recently been identified to cause the inherited immuno-osseous disorder, spondyloenchondrodysplasia (SPENCD). (nih.gov)
  • 1. The microphthalmia transcription factor regulates expression of the tartrate-resistant acid phosphatase gene during terminal differentiation of osteoclasts. (nih.gov)
  • CFU-AP+) and normal numbers of tartrate-resistant acid phosphatase-positive osteoclasts. (jax.org)
  • 3. Serum tartrate-resistant acid phosphatase 5b (TRACP 5b) as a marker of skeletal changes in prostate cancer. (nih.gov)
  • 6. Tartrate-resistant acid phosphatase isoform 5b: a novel serum marker for monitoring bone disease in multiple myeloma. (nih.gov)
  • 8. Tartrate-resistant acid phosphatase 5b as serum marker of bone metabolism in cancer patients. (nih.gov)
  • 11. Serum tartrate resistant acid phosphatase as a potential marker of bone metastasis from breast cancer. (nih.gov)
  • 15. Serum tartrate-resistant acid phosphatase 5b in monitoring bisphosphonate treatment with clodronate: a comparison with urinary N-terminal telopeptide of type I collagen and serum type I procollagen amino-terminal propeptide. (nih.gov)
  • and the dosage of MTX, the use of BP or VitD, and serum tartrate-resistant acid phosphatase 5b (TRACP-5b) in the distal forearm, respectively. (biomedcentral.com)
  • Revealed information recommend sturdy conservation of catalytically necessary amino acids between all 4 proteins and the associated G6PC3 isoform. (molvisindex.org)
  • 4. Mitf-PU.1 interactions with the tartrate-resistant acid phosphatase gene promoter during osteoclast differentiation. (nih.gov)
  • 9. YY1 is involved in RANKL-induced transcription of the tartrate-resistant acid phosphatase gene in osteoclast differentiation. (nih.gov)
  • 12. Involvement of upstream stimulatory factors 1 and 2 in RANKL-induced transcription of tartrate-resistant acid phosphatase gene during osteoclast differentiation. (nih.gov)
  • Moreover, these decreases in osteoblast number can be attributed to the deleterious effects of SAHA on immature osteoblasts, even while mature osteoblasts are resistant to the harmful effects and demonstrate increased activity in vivo, indicating that the response of osteoblasts to SAHA is dependent upon their differentiation state. (elsevierpure.com)
  • Osteocalcin is a small protein (49 amino acids) synthesized by mature osteoblasts, odontoblasts, and hypertrophic chondrocytes. (medscape.com)
  • It is differentiated from other mammalian acid phosphatases by its resistance to inhibition by tartrate and by its molecular weight. (wikipedia.org)
  • Three-dimensional structure of a mammalian purple acid phosphatase at 2.2 A resolution with a mu-(hydr)oxo bridged di-iron center. (cathdb.info)
  • The possible roles of the allantoic sac in metabolism of uteroferrin, the iron-containing, purple phosphatase, were examined. (tamu.edu)
  • Although uteroferrin acid phosphatase activity is stable when incubated in buffer, the enzyme loses activity in sterile allantoic fluid collected at all stages of pregnancy. (tamu.edu)
  • A set of three mechanistically totally different strategies for spheroid well being evaluation (Spheroid quantity, metabolic exercise and acid phosphatase enzyme exercise) have been validated towards cell numbers in wholesome and drug-treated spheroids. (molvisindex.org)
  • Resorption markers, tartrate-resistant acid phosphatase and deoxypyridinoline, increased in the last trimester and after partrition decreased until puerperium day 30. (go.jp)
  • Diagnosis of hairy-cell leukaemia by tartrate-resistant acid phosphatase activity in paraffin-embedded tissue sections. (bmj.com)
  • Tartrate-resistant acid phosphatase activity may be demonstrated in paraffin-embedded liver and spleen specimens as well as in decalcified bone marrow biopsies after fixation in a mixed glutaraldehyde-formaldehyde-calcium acetate solution. (bmj.com)
  • In support of this lineage shift being mediated through altered Wnt signaling, Pb and non-esterified fatty acids in MC3T3 cells increased in vitro PPAR-γ activity and inhibited β-catenin activity. (nih.gov)
  • 13. Tartrate-resistant acid phosphatase 5b activity is a useful bone marker for monitoring bone metastases in breast cancer patients after treatment. (nih.gov)
  • Furthermore, mass spectrometry-based metabolomics and bioenergetics studies identify defects in fatty acid utilization, and alterations in mitochondrial bioenergetics can additionally propel stem cell deficits. (nature.com)
  • Combining Pb and non-esterified fatty acids enhanced these effects. (nih.gov)
  • The cell line is positive for KERATIN, can synthesize LECITHIN, and contains high levels of POLYUNSATURATED FATTY ACIDS in its PLASMA MEMBRANE. (nih.gov)
  • This research describes a practical evaluation of 22 non-synonymous G6PC2 SNPs, that alter amino acids which might be conserved in human G6PC1, mouse G6pc1 and mouse G6pc2, with the aim of figuring out variants that doubtlessly have an effect on G6PC2 exercise/expression. (molvisindex.org)
  • Räisänen SR, Alatalo SL, Ylipahkala H, Halleen JM, Cassady AI, Hume DA, Väänänen HK 2005 Macrophages overexpressing tartrate-resistant acid phosphatase show altered profile of free radical production and enhanced capacity of bacterial killing . (oncobone.com)
  • Two months earlier in Thailand, he had received treatment for hepatosplenic melioidosis with ceftazidime (120 mg/kg/d), TMP/SMX (10/50 mg/kg/d), and doxycycline (200 mg/d) for 15 days, and then oral amoxicillin/clavulanic acid (3 g/d) for 3 months. (cdc.gov)
  • Multiplexing spheroid quantity, resazurin and acid phosphatase viability assays for high-throughput screening of tumour spheroids and stem cell neurospheres. (molvisindex.org)