Tartrate resistant acid phosphatase from osteoclasts. Medical search. Frequent questions

The ACP5 gene provides instructions for making an enzyme called tartrate-resistant acid phosphatase type 5 (TRAP). (medlineplus.gov)
The TRAP enzyme primarily regulates the activity of a protein called osteopontin, which is produced in bone cells called osteoclasts and in immune cells. (medlineplus.gov)
Two versions (isoforms) of the TRAP enzyme are produced: TRAP5a is found primarily in immune cells and TRAP5b is found primarily in bone cells called osteoclasts. (medlineplus.gov)
The ACP5 gene mutations that cause SPENCDI typically change single protein building blocks (amino acids) in the TRAP enzyme or result in the production of an abnormally short enzyme. (medlineplus.gov)
Tartrate-resistant acid phosphatase (TRAP) and the osteoclast/immune cell dichotomy. (medlineplus.gov)
Tartrate-resistant acid phosphatase (TRAP) is a basic iron-binding protein with high activity towards phosphoproteins, ATP and 4-nitrophenyl phosphatase. (thermofisher.com)
However, tartrate-resistant acid phosphatase (TRAP) activity and the number of osteoclast cells (TRAP-positive multinucleated cells) were not changed by nSP treatment in the presence of receptor activator of nuclear factor κB ligand (RANKL) at doses that did not induce cytotoxicity by silica particles. (springer.com)
RANKL led to the formation of osteoclasts identified as tartrate-resistant acid phosphatase (TRAP)-positive multinucleated cells in the culture of RAW 264.7 cells. (nih.gov)
TRAP, tartrate-resistant acid phosphatase. (silverchair.com)
10 - 12 ] c-Fos also induces the expression of NFATc1, contributing to the induction of osteoclast-specific marker genes such as tartrate-resistant acid phosphatase ( TRAP ). (e-jbm.org)
We prepared root-surrounding tissues with (R) or without (N) physiological root resorption scraped off at three regions (R1-R3 or N1-N3) from the cervical area to the apical area of the tooth and measured both TGF-β and the tartrate-resistant acid phosphatase (TRAP) activities. (preprints.org)
In vitro experiments for RANKL-mediated osteoclast differentiation revealed that TGF-β in N1-N3 and R1-R3 enhanced the TRAP activity in RAW264 cells. (preprints.org)
After 15 days, we performed osteoclasts identification through tartrate resistant acid phosphatase (TRAP) staining and osteoclasts activity assessment through TRAF6, NFATC1 and Cathepsin K gene expression. (unina.it)
Cathepsins and tartrate resistant acid phosphatase (TRAP) are considered key enzymatic factors contributing to bone erosion. (uni-mysore.ac.in)
SHH upregulated phosphorylation of ERK1/2 and p38 MAPK, NFATc1, tartrate-resistant acid phosphatase (TRAP), and Cathepsin K expression in RAW264.7 cells. (vectorbiolabs.com)
The expression of alkaline phosphatase (ALP) was assessed by immunohistochemistry and the number of osteoclasts was shown by Tartrate-resistant acid phosphatase (TRAP) staining. (imr.ac.cn)
It has been shown that multinucleated foreign body giant cells also express tartrateresistant acid phosphatase (TRAP) [14], hence, classical TRAP staining is unable to distinguish between macrophage polykaryons and osteoclasts. (sciforschenonline.org)
After 7 days, the rats were sacrificed and each maxilla processed for histological examination and stained for osteoclastic activity using tartrate-resistant acid phosphatase (TRAP). (edu.au)
TRAP staining revealed decreased osteoclasts number in SONFH mice after APS treatment. (biomedcentral.com)
During the process of osteoclast generation, bone marrow macrophages (BMMs) differentiate into tartrate-resistant acid phosphatase (TRAP)-positive pre-osteoclasts, which then fuse with each other to form mature osteoclasts. (tech-strategy.org)
Experiments coculturing mouse macrophages (RAW264.7) with AM-1 demonstrated low osteoclastogenic activity, as assessed by tartrate-resistant acid phosphatase (TRAP)-positive multinuclear cell formation, probably because of low release of RANKL, whereas cocultures of RAW264.7 with HSC-3 cells exhibited very high osteoclastogenic activity. (elsevierpure.com)
We evaluated the role of CB2 and TRPV1 receptors and of iron in the development of OP in pediatric IBD at diagnosis and analyzed the effects of the pharmacological modulation of CB2 and TRPV1 receptors on osteoclast activity and on iron metabolism by Western Blotting, Tartrate-resistant acid phosphatase assay (TRAP assay) and Iron Assay Kit. (unicampania.it)
In IBD patients we found a significant up-regulation of osteoclast markers TRAP and Cathepsin K and a consequent osteoclast hyper-activation. (unicampania.it)

Therefore, inhibition of RANKL-induced osteoclastogenesis and osteoclast-induced bone resorption is an important approach in controlling pathophysiology of these skeletal diseases. (hindawi.com)
We found that, of seven type I, II, and III saikosaponins isolated from Bupleurum falcatum , saikosaponins A and D, type I saikosaponins with an allyl oxide linkage between position 13 and 28 and two carbohydrate chains that are directly attached to the hydroxyl groups in position 3, exhibited the most potent inhibition on RANKL-induced osteoclast formation at noncytotoxic concentrations. (hindawi.com)
Saikosaponins A and D inhibited the formation of resorptive pits by reducing the secreted levels of matrix metalloproteinase- (MMP-) 2, MMP-9, and cathepsin K in RANKL-induced osteoclasts. (hindawi.com)
Collectively, the results suggested that nanosilica exerts no effect on RANKL-induced osteoclast differentiation of RAW264.7 cells, although a detailed mechanistic examination of the nSP70-mediated cytotoxic effect is needed. (springer.com)
The effect of pyrroloquinoline quinine (PQQ) on receptor activator of nuclear factor-κB ligand (RANKL)-induced osteoclast formation was examined using RAW 264.7 macrophage-like cells. (nih.gov)
However, PQQ inhibited the appearance of osteoclasts and prevented the decrease of F4/80 macrophage maturation marker on RANKL-stimulated cells, suggesting a preventive action of PQQ on RANKL-induced osteoclast differentiation. (nih.gov)
Taken together, PQQ was suggested to prevent RANKL-induced osteoclast formation via the inactivation of NFATc1 by reduced c-Fos expression. (nih.gov)
Expression of RANKL correlated with the number of osteoclasts (r = 0.592, p = 0.004). (bmj.com)
The increased number of osteoclasts and RANKL expression in samples with MRI bone oedema supports the hypothesis that bone erosion in RA occurs through activation of local bone resorption mechanisms within subchondral bone as well as through synovial invasion into bone. (bmj.com)
In culture, RANKL-expressing B1a cells significantly promoted the expansion of osteoclasts derived from bone marrow cells, which were in accord with the in vivo findings of increased osteoclastogenesis in CIA mice transferred with B1a cells. (oncotarget.com)
EFV reduced RANKL-mediated osteoclast formation and activation by inhibiting expression of nuclear factor of activated T cells 1, a key factor of osteoclastogenesis. (e-jbm.org)
Osteoclast formation is controlled by 2 cytokines, macrophage colony-stimulating factor (M-CSF) and receptor activator of nuclear factor-κB ligand (RANKL), which are secreted by osteoblasts/activated T cells. (e-jbm.org)
The binding of RANKL, the critical cytokine for osteoclast differentiation, to its receptor in osteoclast precursor cells of the monocyte lineage recruits the adaptor protein TRAF6 and activates multiple signaling pathways. (bvsalud.org)
Here, we examined whether ECSIT could be implicated in the rapid effects of RANKL and E2 on osteoclast progenitors. (bvsalud.org)
Instead, RANKL stimulated apoptosis of osteoclast progenitors. (bvsalud.org)
Osteoclasts were formed by culturing mouse bone marrow macrophage (BMM) cells with macrophage colony-stimulating factor and receptor activator of nuclear factor-κB ligand (RANKL). (e-jbm.org)
In BMM cultures, an ethanol extract of the root part of IKN suppressed RANKL-induced osteoclast formation and bone resorptive activity. (e-jbm.org)
In contrast, an ethanol extract of the aerial parts of IKN had a minor effect on RANKL-induced osteoclast formation. (e-jbm.org)
5 ] Osteoblasts, bone-forming cells, also regulate osteoclast differentiation via RANKL and osteoprotegerin (OPG) expression, a decoy receptor for RANKL. (e-jbm.org)
5 , 6 ] Stimulation of osteoclast precursors with RANKL recruits cytoplasmic tumor necrosis factor receptor-associated factors (TRAFs) and activates downstream signaling pathways. (e-jbm.org)
We show that H3 monomethylation at lysine 27 (H3K27me1) is essential for MMP-9-dependent H3NT proteolysis during RANKL-induced osteoclast differentiation. (biomedcentral.com)
The binding of RANKL to its cognate receptor RANK on pre-osteoclast cell membrane stimulates the expression of key determinants of osteoclast differentiation such as NF-κB, c-Fos, and NFATc1 at the early stage of the process [ 1 , 8 ]. (biomedcentral.com)
Studies have showed the key role played by osteoclasts and receptor activator of nuclear factor kappaB ligand (RANKL) signaling in bone erosion. (uni-mysore.ac.in)
The inflammatory cytokines and ROS influence osteoclast differentiation by regulating osteoclast-lineage cells or by acting on other cells to regulate the expression of RANKL and osteoprotegerin (OPG). (uni-mysore.ac.in)
Currently, the clinical interventions for patients with BC-bone metastasis (BC-BM) are bisphosphonates (BPs) or denosumab (an anti-receptor activator of nuclear factor kappa B ligand (RANKL) monoclonal antibody), which disrupt the vicious cycle by targeting osteoclasts [ 6 ]. (biomedcentral.com)
SHH stimulated osteoclast formation and pit formation in the presence of the receptor activator for nuclear factor-¿B ligand (RANKL) in CD11b+ mouse bone marrow cells. (vectorbiolabs.com)
Osteoclasts and odontoclasts are multinucleated cells derived from hematogenous bone marrow, from the monocyte/macrophage cell lineage, and are formed by the fusion of these cells under the action of colony stimulating factor 1 (CSF-1), macrophage colony stimulating factor (M-CSF), monocyte chemotactic protein-1 (MCP-1) and RANKL (receptor activator of nuclear factor kappa-B ligand), which also have a role in chemotaxis and cell differentiation [1]. (oatext.com)
The differentiation of the osteoclast precursor into a mature osteoclast requires RANKL binding to its receptor RANK (receptor activator of nuclear factor kappa-B). However, OPG binding to RANKL will block RANKL binding to RANK, which will result in inhibition of osteoclastogenesis (Figure 1) [3]. (oatext.com)
In both cases, the cells need to be differentiated into the mature osteoclast by M-CSF and RANKL [1-4]. (sciforschenonline.org)
Recently, this cell line has become a valuable tool to study osteoclast differentiation and activity due to its expression of RANK and differentiation to osteoclast in response to RANKL [7]. (sciforschenonline.org)
In addition to its effects on osteoblasts, Wnt/-catenin signaling decreases osteoclast differentiation by stimulating the production and secretion of osteoprotegerin (OPG) [19] C a natural antagonist of the receptor activator of nuclear factor-B ligand (RANKL) [20]. (tech-strategy.org)
RANKL and macrophage colonyCstimulating factor, provide the two necessary and sufficient signals for osteoclast differentiation [22]. (tech-strategy.org)
It is believed that it expands in the jaw bone through peritumoral activation of osteoclasts by receptor activator of nuclear factor kappa-B ligand (RANKL) released from the ameloblastoma, as in bone metastases of cancer cells. (elsevierpure.com)
Thus, RANKL release from AM-1 appeared to be too low to generate osteoclasts. (elsevierpure.com)

It is detected in human alveolar macrophages, osteoclasts, spleen and liver. (thermofisher.com)
Many types of cells are involved in the progression of RA joint inflammation, among which the overactivation of M1 macrophages and osteoclasts has been thought to be an essential cause of joint inflammation and bone destruction. (elifesciences.org)
By pharmacological inhibition of GLI1, the proportion of proinflammatory macrophages and the number of osteoclasts were significantly reduced, and the joint inflammatory response and bone destruction in CIA mice were alleviated. (elifesciences.org)
This study presents an important finding on the role of GLI1 in macrophages and osteoclasts in the pathogenesis of inflammatory arthritis, and suggests a therapeutic potential of GLI1 targeting in rheumatoid arthritis. (elifesciences.org)
EFV), a genus of lichenized fungi within the Parmeliaceae , on the differentiation of bone marrow-derived macrophages (BMMs) into osteoclasts was examined by phenotype assay and the cell cytotoxicity was evaluated by cell counting kit-8. (e-jbm.org)
Although these protocols provide a successful method to culture osteoclasts from RAW264.7 cells, they don't express the issue of having multinucleated cells such as macrophages mixing with osteoclasts in the population. (sciforschenonline.org)

Under healthy physiological conditions, activities of osteoclasts and osteoblasts are tightly controlled by both systemic and local factors, maintaining bone mineral density and bone microarchitecture. (hindawi.com)
However, an imbalance in osteoclasts and osteoblasts activities, mostly in favor of a hyperosteoclast activity, is responsible for pathophysiology of several skeletal diseases, including osteoporosis and metastatic bone diseases [ 1 ]. (hindawi.com)
Osteoclasts and osteoblasts work in turn to remove old, damaged bone and then replace it, resulting in strong bones that are in a continuous process of renewal. (saveourbones.com)
When osteoclasts outpace osteoblasts, it causes a loss of bone mass and with it a loss of the structural quality of bone. (saveourbones.com)
The opposite problem can also occur, in which osteoblasts outpace osteoclasts- resulting in new bone deposited over old and damaged bone. (saveourbones.com)
The material that osteoblasts deposit into the pits left by osteoclasts takes time to reach full strength and integration into the existing bone. (saveourbones.com)
However, active bone remodeling with osteoclasts and osteoblasts working in synchrony continues to occur. (elsevier.es)
This process requires a balanced action of bone-resorbing osteoclasts and bone-forming osteoblasts. (e-jbm.org)
Bone is a unique connective tissue which constantly undergoes remodeling, which involves bone formation by osteoblasts and bone resorption by osteoclasts[ 3 ]. (ijbs.com)
Bone is a highly dynamic organ that is continuously remodeled by coordinated activities of two cell types, osteoclasts and osteoblasts [ 1 , 2 ]. (biomedcentral.com)
Osteoblasts are mononucleated mesenchymal stem cells that form bone matrix, whereas osteoclasts are bone-resorbing multinucleated cells that differentiate from hematopoietic progenitors of the myeloid lineage [ 3 , 4 , 5 ]. (biomedcentral.com)
In addition to their indirect effects on osteoclastogenesis that are mediated by controlling OPG expression and secretion by osteoblasts/osteocytes, Wnts act directly on osteoclasts. (tech-strategy.org)

Silencing of ECSIT decreased osteoclast differentiation and abrogated the inhibitory effects of E2 on osteoclastogenesis. (bvsalud.org)

The decreased expression and inhibition of nuclear transfer of GLI1 downregulated macrophage M1 polarization and osteoclast activation, the effect of which was achieved by modulation of DNA methyltransferases (DNMTs) via transcriptional regulation and protein interactions. (elifesciences.org)
This study clarified the mechanism of GLI1 in macrophage phenotypic changes and activation of osteoclasts, suggesting potential applications of GLI1 inhibitors in the clinical treatment of RA. (elifesciences.org)
Osteoclasts originate from monocyte/macrophage lineage cells. (e-jbm.org)
They don't often exclusively differentiate into osteoclast but also into other multinucleated cells as well including macrophage polykaryons. (sciforschenonline.org)
However, osteoclasts and macrophage polykaryons are almost indistinguishable under a light microscope (TRAP+ with three or more nuclei). (sciforschenonline.org)
nevertheless, we are able to identify the optimal conditions at which osteoclasts are found to be more than macrophage polykaryons. (sciforschenonline.org)
Finally, this article also demonstrates that osteoclasts and macrophage polykaryons can be distinguished by immunofluorescence staining for cathepsin K. (sciforschenonline.org)
Osteoclasts can be obtained by isolating primary bone marrow monocytes or by using macrophage cell lines. (sciforschenonline.org)

The following genes encode the polypeptide components for various acid phosphatase isoenzymes:[citation needed] ACP1 ACP2 ACPP (ACP3), prostatic acid phosphatase ACP5, tartrate-resistant acid phosphatase ACP6 ACPT, testicular acid phosphatase Tissue acid phosphatase, or lysosomal acid phosphatase Alkaline phosphatase Henneberry MO, Engel G, Grayhack JT (October 1979). (wikipedia.org)
The osteoclast differentiation-related genes and proteins were investigated by real-time polymerase chain reaction and immunoblotting. (e-jbm.org)
Through the recognition of H3K27me1 mark, MMP-9 localizes and generates H3NT proteolysis at the genes encoding osteoclast differentiation factors. (biomedcentral.com)
By using RNAi and small molecule inhibitor approaches, we also confirmed that G9a is the major methyltransferase to catalyze H3K27me1 for MMP-9-dependent H3NT proteolysis and trigger the expression of osteoclast-specific genes. (biomedcentral.com)
These factors then initiate multiple signal transduction pathways to turn on downstream genes and activate quiescent osteoclast precursor (OCP) cells to become mature osteoclasts [ 1 , 8 ]. (biomedcentral.com)

Therefore, we consider that it is necessary to estimate nSP-induced effects on osteoclast differentiation. (springer.com)

The two we can measure are tartrate-resistant acid phosphatase and cathepsin K. (saveourbones.com)
Furthermore, specifically knock out 11β-HSD1 in osteoclasts, by crossing cathepsin-cre mice with 11β-HSD1 flox/flox mice, presented significant protecting effect of skeleton when they underwent ovariectomy surgery. (ijbs.com)
Similarly the activation markers of mature osteoclasts, TRAF6, NFATC1 and Cathepsin K, were enhanced. (unina.it)

In this study, we found that EFV, a genus of lichenized fungi within the Parmeliaceae , inhibited osteoclast formation. (e-jbm.org)

15] demonstrated the importance of L-Gln for osteoclast differentiation of murine BMMs. (sciforschenonline.org)

HGF is known to play a role in osteoclast formation, and in this study we have determined whether HGF could replace M-CSF to support human osteoclastogenesis. (ox.ac.uk)

The possible mechanisms involved in osteoclasts recruitment from external granuloma and various dentin resorption patterns in internal root resorption will be discussed based on a case in which the upper lateral incisor with a history of pulp necrosis treated endodontically, developed an internal root resorption in association with external granuloma. (oatext.com)
Osteoclasts recruitment and activation, and angiogenesis in maxillary alveolar processes in internal root resorption associated with external granuloma. (oatext.com)

Our studies revealed that nSP induced high ROS and pro-inflammatory cytokine production, and these cellular responses may induce excess osteoclast differentiation. (springer.com)
Supplementation of osteoclast cultures from normal and XLA subjects with serum from XLA patients or recombinant inflammatory cytokines IL-6, IL-1β, and TNF-α resulted in a stimulation of osteoclast activity in vitro, whereas the addition of cytokine-neutralizing antibodies inhibited this stimulatory effect, confirming that elevated inflammatory cytokines in XLA serum heightened osteoclast activity in vitro. (uea.ac.uk)
In XLA patients, however, these inherent osteoclast defects are corrected by increased inflammatory cytokine levels, restoring osteoclast activity and leading to the normalization of bone density. (uea.ac.uk)

The urinalysis and serum calcium phosphate, alkaline phosphatase and parathyroid hormone were within their reference ranges. (pathologyoutlines.com)

Our study suggested that the ethanol extract of the root part of IKN suppressed osteoclast differentiation and function partly by downregulating the p38 MAPK/c-Fos/NFATc1 signaling pathways. (e-jbm.org)

Finally, transcriptome sequencing of 11β-HSD1 knock in osteoclast progenitor cells indicated the Hippo pathway1 was mostly enriched. (ijbs.com)
On the other hand, the hedgehog receptor Patched and the signaling molecule Gli-2 were highly expressed in the osteoclasts and the progenitor cells. (vectorbiolabs.com)

In this study, we show that human Btk-deficient osteoclasts are defective at resorption activity in vitro owing to a dysregulation of the actin cytoskeletal function. (uea.ac.uk)
In this study, we aimed to demonstrate the effects of IKN on osteoclast differentiation in vitro and in vivo . (e-jbm.org)
In vitro experiments showed the endogenous high expression of 11β-HSD1 lead to osteoclast formation and maturation. (ijbs.com)

The following product was used in this experiment: Tartrate Resistant Acid Phosphatase Monoclonal Antibody (5C5E7) from Thermo Fisher Scientific, catalog # MA5-38435, RRID AB_2898349. (thermofisher.com)

Tartrate-resistant acid phosphatase deficiency causes a bone dysplasia with autoimmunity and a type I interferon expression signature. (medlineplus.gov)

Background: Osteoprotegerin (OPG), as an osteoclast antagonist, limits mineralised tissue resorption under physiological conditions. (edu.au)

Osteoclasts are specialized cells that break down and remove (resorb) bone tissue that is no longer needed. (medlineplus.gov)
As a result, osteopontin is abnormally active, prolonging bone breakdown by osteoclasts and triggering abnormal inflammation and immune responses by immune cells. (medlineplus.gov)
In the process of bone turnover cells called osteoclasts dissolve discrete portions of bone, transferring minerals like calcium and magnesium from the bone tissue into the blood. (saveourbones.com)
Cells called osteoclasts dissolve portions of bone, resorbing minerals into the blood. (saveourbones.com)
Lymphocytic aggregates containing CD45RO+ T cells and B cells within bone marrow have been identified in subchondral bone in RA, 8 and a recent immunohistochemical study reported an association between bone marrow inflammation and osteoclasts in RA subchondral bone. (bmj.com)
Microscopic examination revealed fibroblastic stroma with spindled mesenchymal cells and multinucleated osteoclast-like giant cells ( Figure 2 ). (pathologyoutlines.com)
In addition, the pathological process of RA is promoted through the synergistic action of the cellular resident in the bone and in joint compartments, such as osteoclasts, chondrocytes and stromal cells ( Komatsu and Takayanagi, 2022 ). (elifesciences.org)
Osteoclasts are multinucleated giant cells that can resorb bone. (e-jbm.org)
6 ] Osteoclasts are large multinucleated cells (MNCs) that remove the old/weakened bones by acid decalcification and proteolytic degradation. (e-jbm.org)
Estrogens protect against bone loss by decreasing osteoclast number through direct actions on cells of the myeloid lineage. (bvsalud.org)
Osteoclasts are unique cells that resorb old bones. (e-jbm.org)
Objective Explore euscaphic acid uptake and transport mechanisms in Caco-2 cells. (alljournal.net.cn)
4. Conclusions Euscaphic acid has good transmembrane permeability in Caco-2 cells, and the uptake mode is mainly passive diffusion. (alljournal.net.cn)
showed that the osteoclastic potential of mandibular bone marrow stromal cells and the number of osteoclasts are higher compared to long-bone marrow stromal cells [2]. (oatext.com)
Currently published protocols of culturing RAW264.7 cells often assume multinucleated cells that have three or more nuclei with distinguished osteoclast characteristics (such as TRAP+) as osteoclasts. (sciforschenonline.org)
Osteoclasts are multinucleated cells that are essential for bone resorption and regulate bone remodeling. (sciforschenonline.org)
The demineralization activity of AM-1 was only 1.7% of osteoclasts demineralization activity, and the growth rate was 20% of human normal skin keratinocytes and HSC-3 cells. (elsevierpure.com)
HGF was able to support monocyte-osteoclast differentiation in the presence of receptor activator for nuclear factor kappaB ligand as evidenced by the formation of numerous multinucleated tartrate-resistant acid phosphatase and vitronectin receptor positive cells which formed F-actin rings and were capable of lacunar resorption. (ox.ac.uk)

Osteoclastic enzymes are secreted by osteoclasts to break down bone matrix proteins. (saveourbones.com)
Previous work investigating OPG in a rat periodontal ligament (PDL) ankylosis model found no inhibitory effect on osteoclasts when OPG was administered at a dosage of 2.5mg/kg.1,2 Aims: The object of this study was to determine whether dosages higher than 2.5 mg/kg of OPG were required to limit osteoclastic activity in an aseptic inflammatory model in rats. (edu.au)
Conclusion: Although osteoclastic activity reduced, it was not completely eliminated, perhaps because dosages were still inadequate, or additional factors might influence OPG and osteoclast activation in the aseptic inflammatory model. (edu.au)

Some plant roots, especially cluster roots, exude carboxylates that perform acid phosphatase activity, helping to mobilise phosphorus in nutrient-deficient soils. (wikipedia.org)
The main cause of this symptoms is excessive increase in the number and activity of osteoclasts. (e-jbm.org)
The functional activity of osteoclast in response to EFV treatment was evaluated by an Osteo Assay plate. (e-jbm.org)
5 ] Typical bone diseases, such as osteoporosis, Paget's disease, and rheumatoid arthritis, have the symptoms of decreased bone mass because of the increased number and activity of osteoclasts. (e-jbm.org)
Osteoclasts and their activity are key regulators of bone formation. (sciforschenonline.org)

Different forms of acid phosphatase are found in different organs, and their serum levels are used to evaluate the success of the surgical treatment of prostate cancer. (wikipedia.org)
This glycoprotein stimulates osteoclast-mediated bone resorption and can be measured in blood serum. (saveourbones.com)

Osteoclasts that resorb bone play an important role in bone remodelling. (springer.com)

Conclusion Our data confirm the well-known role of CB2 and TRPV1 receptors in bone metabolism and suggest that their stimulation can reduce the osteoclast overactivity induced by iron, thus providing new insights into the pathogenesis and in the treatment of pediatric IBD-related bone resorption. (unicampania.it)

This study provides novel evidence that Btk signaling is crucial for optimal actin cytoskeletal organization and lacunar resorption in isolated osteoclasts. (uea.ac.uk)

The RAW264.7 cell line is most commonly used to study osteoclast differentiation and its signaling pathways. (sciforschenonline.org)

Tartrate-resistant acid phosphatase may be used as a biochemical marker of osteoclast function during the process of bone resorption. (wikipedia.org)

For physiological or pathological understanding of bone disease caused by abnormal behavior of osteoclasts (OCs), functional studies of molecules that regulate the generation and action of OCs are required. (molcells.org)

Studies suggest that iron overload affects the bone, accelerating osteoclasts (OCs) differentiation and activation with consequent bone resorption. (unicampania.it)

HGF is a well-established survival factor and viability assays and live/dead staining showed that it promoted the survival and proliferation of monocytes and osteoclasts in a manner similar to M-CSF. (ox.ac.uk)

Conclusions Sodium butyrate can alleviate diarrhea and colon visceral hypersensitivity in IBS-D rats by improving the imbalance in the gut microbiota-short-chain fatty acid metabolic axis. (alljournal.net.cn)

1 ] Osteoclasts play critical roles in many bone-destructive diseases such as osteoporosis, osteomyelitis, rheumatoid arthritis, and periodontitis. (e-jbm.org)
In conclusion, our study demonstrated the role of 11β-HSD1 in facilitating osteoclasts formation and maturation through the Hippo signaling, which is a new therapeutic target to manage osteoporosis. (ijbs.com)
Dysregulation of osteoclasts can cause bone diseases such as osteopetrosis and osteoporosis. (sciforschenonline.org)

Objective To explore the alterations in the gut microbiota-short-chain fatty acid metabolism axis in a rat model of diarrhea-predominant irritable bowel syndrome (IBS-D), and to explore the effect of sodium butyrate on this axis. (alljournal.net.cn)

Meanwhile, we found 11β-HSD1 facilitated mature osteoclasts formation inhibited bone formation coupled H type vessel (CD31 hi Emcn hi ) growth through reduction of PDFG-BB secretion. (ijbs.com)

p38, c-Jun N-terminal kinase, extracellular signal-regulated kinase), and phosphoinositide 3-kinase/Akt serine/threonine kinase (Akt) signaling pathways are known to be activated during osteoclast differentiation. (e-jbm.org)

Lipopolysaccharide (LPS)-induced osteoclast formation was performed using a mouse calvarial model. (e-jbm.org)
IKN administration decreased LPS-induced osteoclast formation in a calvarial osteolysis model in vivo . (e-jbm.org)
Then, by suppression of YAP expression in Hippo signaling, we observed the redundant of osteoclasts formation even in 11β-HSD1 high expression conditions. (ijbs.com)
Our results suggest that tumor-derived SHH stimulated the osteoclast formation and bone resorption in the tumor jawbone microenvironment. (vectorbiolabs.com)
Our findings indicate that HGF can substitute for M-CSF to support human osteoclast formation. (ox.ac.uk)

Further, reactive oxygen species (ROS) formed at the ruffled border of osteoclasts also causes bone resorption and matrix degradation. (uni-mysore.ac.in)

Anatomy2

Diseases1

Chemicals and Drugs18

Phenomena and Processes2

Information Science1

TRAP23

RANKL34

Macrophages6

Osteoblasts12

Decreased osteoclast differentiation1

Macrophage8

Genes5

Effects on osteoclast differentiation1

Cathepsin3

Inhibited osteoclast formation1

BMMs1

Osteoclastogenesis1

Root resorption2

Cytokine3

Alkaline1

NFATc11

Progenitor cells2

Vitro3

Monoclonal Antibody1

Deficiency1

Osteoprotegerin1

Cells18

Osteoclastic3

Activity5

Serum2

Resorb bone1

Stimulation1

Actin1

RAW264.71

Process of bone1

Regulate1

Activation1

Monocytes1

Conclusions1

Osteoporosis3

Metabolism1

Bone formation1

Pathways1

Formation5

Oxygen1