Targets for anticancer hepatocellular carcinoma cells micro rna that regulates. Medical search. Frequent questions

microRNAs (miRNAs) are short (20-24 nt) non-coding RNAs that are involved in post-transcriptional regulation of gene expression in multicellular organisms by affecting both the stability and translation of mRNAs. (wikipedia.org)
miRNAs are transcribed by RNA polymerase II as part of capped and polyadenylated primary transcripts (pri-miRNAs) that can be either protein-coding or non-coding. (wikipedia.org)
Dysregulation of miRNAs is a common feature in human cancers, but this phenomenon has not been studied extensively in hepatocellular carcinoma (HCC). (spandidos-publications.com)
Recently, an increasing number of reports have implicated a new class of small regulatory RNA molecules, termed microRNAs (miRNAs), in HCC progression. (spandidos-publications.com)
A recent demonstration of the differential expression of miRNAs and their target mRNAs in cancer and the discovery that some miRNAs can function as oncogenes or tumor suppressors have sparked considerable interest in elucidating their role in tumorigenesis ( 5 , 6 ). (spandidos-publications.com)
miRNAs are highly conserved, small, non-coding RNAs that negatively regulate gene expression in vertebrates through multiple mechanisms, such as complimentary base pairing with the 3′-UTR of their target mRNAs, resulting in translational repression, mRNA cleavage and mRNA decay initiated by miRNA-guided rapid deadenylation ( 10 ). (spandidos-publications.com)
However, recent studies have demonstrated that miRNAs can interact with the 5′-UTR of their target mRNAs and the DNA methylation machinery, thereby affecting chromatin status ( 11 ). (spandidos-publications.com)
MicroRNAs (miRNAs) play vital roles in regulating various biological processes. (biomedcentral.com)
MiRNAs (miRNAs) are small non-coding RNAs which are recognized as vital and evolutionarily ancient components of gene regulation [ 1 ]. (biomedcentral.com)
For detecting and quantifying the expression of specific miRNAs, RNA was reverse transcribed using a Bulge-Loop™ miRNA qRT-PCR Primer Set and quantified by SYBR Green-based real-time PCR analysis in the FTC-3000P PCR instrument. (biomedcentral.com)
microRNAs (miRNAs) have been emerged as a class of novel noncoding RNA regulator, and their length are approximately 22 nucleotides in length [ 4 ]. (hindawi.com)
In recent years, non-coding RNAs (ncRNAs) have gradually become a hotspot in EV-RNA research, especially EV-miRNAs, which have played a vital role in intercellular communication. (molcells.org)
Our study uncovered a novel molecular mechanism of lncRNA SNHG4 in driving prostate cancer progression and enzalutamide resistance, revealing the critical roles and therapeutic potential of RREB1, SNHG4, RRM2 and let-7 miRNAs in anticancer therapy. (biomedcentral.com)
Mechanistically, lncRNAs have been characterized as sponges with microRNAs (miRNAs) or circular RNAs (circRNAs) and act as competing endogenous RNAs (ceRNAs) to reduce the regulation of their target genes [ 15 ]. (biomedcentral.com)
Previous studies have shown that miRNAs can regulate the proliferation, invasion, and migration of cancer cell-related processes. (viamedica.pl)
In recent years, emerging studies have highlighted the critical role of these pathways and their regulation by microRNAs (miRNAs) in cancer invasion and metastasis. (intechopen.com)
MiRNAs, short (18-24 nucleotides) non-coding RNAs, are derived from long transcripts pri-miRNAs and pre-miRNAs [ 26 - 30 ].By targeting 3' untranslated regions (3'UTRs) of cognate mRNAs, miRNAs post-transcriptionally regulate gene expression and induce translational repression [ 29 , 30 ]. (intechopen.com)
To date, 1527 human miRNAs have been identified (Sanger miRBase 18 http://www.miRbase.org/index.shtml), forming less than 1% of all human genes, potentially regulating more than 10% of all protein coding genes [ 1 ]. (intechopen.com)
We highlight the role of SE-driven genes, enhancer RNAs (eRNAs), lncRNAs, and miRNAs in the digestive system tumor growth and progression. (ijbs.com)
Despite the well-known fact that primiRNAs are acting as non-protein-coding RNAs, the published data suggest that, in the plant genomes, some pri-miRNAs can also be found in polysomes, and the expression of these miRNA precursors may results in formation of micropeptides which may be involved in regulation of gene expression. (fortunepublish.com)
Previously, non-coding RNAs (ncRNAs), including micro-RNAs (miRNAs) and other long non-coding RNAs (lncRNAs), have been generally considered unable to encode proteins both in plants and animals [1-9]. (fortunepublish.com)
Current debates in the field include the importance of miRNAs in general and their utility as targets in preventing tumorigenesis (Blood 119:513-520, 2012). (biomedcentral.com)
MicroRNAs (miRNAs) are short (about 21 nucleotides long) strands of non-coding RNA that regulate the expression of multiple genes [ 1 ]. (biomedcentral.com)
The results of the miRNA-prognostic DEPRG-TF regulatory network showed that GSDMC, IRF1, and PLCG1 were regulated by both specific and common miRNAs and TFs. (hindawi.com)

Previous studies revealed that inhibition of miR-744-3p could restore PTEN expression, and then suppressed laryngeal squamous cell carcinoma progression [ 20 ], suggesting that upregulation of PTEN could contribute to cancer development. (hindawi.com)
Compared with squamous cell carcinoma and large cell carcinoma, adenocarcinoma is the most common type of NSCLC due to its high incidence and rapid metastasis. (molcells.org)
NSCLC mainly includes the histologic types of adenocarcinoma (ADC) and squamous cell carcinoma (SCC) [ 2 ]. (jcancer.org)
Abstract Oral squamous cell carcinoma (OSCC) is a malignant tumor triggered by the accumulation of multiple gene mutations in oral epithelial cells. (techscience.com)

Cancer aggressiveness, metastasis, drug resistance, cancer stem cell self-renewal/stemness, and disease relapse are some of the critical problems in the management of the disease. (edu.in)
The crosstalk between pro-tumorigenic immune cells, stromal cells, and cytokines helps to establish the pre-metastatic niche for disseminated circulatory tumor cells and facilitates metastasis. (biomedcentral.com)
During metastasis, tumor-derived exosomes help in the requirement and arrangement of immunosuppressive immune cells for favorable premetastatic niche formation and growth of metastases. (biomedcentral.com)
The effect of pristimerin on PC-3 stem cell characteristics and metastasis were detected by spheroid formation, CD133 and CD44 protein expression, matrix-gel invasive assay and colony-formation assay in vitro , VEGF and pro-inflammatory cytokines expression by ELISA assay, and tumor tumorigenicity by X-ray and MR in NOD-SCID mice model in vivo . (stemcellsciencenews.com)
We explored molecular markers shared between TDEs and circulating tumor cells (CTCs) in the blood of cancer patients to identify candidate genes involved in CRC metastasis. (researchsquare.com)
AP4 presumably contributes to the phenotype of cancer cells by activating or repressing genes that harbor CAGCTG elements in their promoter regions, thereby controlling processes such as proliferation, metabolism, apoptosis, epithelial-mesenchymal transition (EMT) and metastasis [ 5 ]. (biomedcentral.com)

Importantly, we demonstrate that the overexpression of miR-148a significantly inhibits HCC cell proliferation and cell cycle progression. (spandidos-publications.com)
The progression of hepatocellular carcinogenesis is thought to involve the deregulation of genes that are critical to cellular processes, such as cell cycle control, cell growth, apoptosis, cell migration and spreading. (spandidos-publications.com)
This miRNA functions as a tumor suppressor and dysregulation or loss of the host gene from which this miRNA is processed is associated with cancer progression in numerous cell types. (cancerindex.org)
Although microRNA-29a-3p was reported to inhibit laryngocarcinoma progression, the potential mechanisms have not been explored clearly. (hindawi.com)
MiR-29a-3p was enriched in hBMSC-derived Exo, and the Exo from miR-29a-3p mimics transfected hBMSCs could inhibit laryngocarcinoma cell malignant phenotypes in vitro and prevent tumor progression in vivo . (hindawi.com)
In conclusion, hBMSC-derived Exo with upregulated miR-29a-3p inhibited laryngocarcinoma progression via regulating PTEN, providing a potential diagnostic and therapeutic target in this disease. (hindawi.com)
It is a catalytic subunit of the protein kinase complex that is important for cell cycle G1 phase progression. (thermofisher.com)
It has been shown that MYL9 promotes cancer cell migration and is implicated in tumor development and progression. (cusabio.com)
LncRNA AGPG has been shown to play a role in esophageal cancer by binding to and stabilizing PFKB3, which increases glycolytic flux and cell cycle progression in esophageal cancer cells. (ijbs.com)
The down-regulation of miR-874-3p recovered the reduction in cell invasion and proliferation in vitro which were induced by CASC9 knockdown and delayed the tumor progression in vivo. (biomedcentral.com)
To develop effective targeted therapies for OS, extensive studies on the molecular mechanisms regulating the progression of OS are quite necessary. (biomedcentral.com)
Aberrant changes in epigenetic mechanisms regulating DNA methylation, histone methylation and acetylation, expression of noncoding RNAs, and mRNA methylation are associated with the initiation, growth, and progression of digestive system tumors [ 2 , 4 ]. (ijbs.com)
Xu F , Guan Y , Ma Y , Xue L , Zhang P , Yang X , Chong T , . Bioinformatic analyses and experimental validation of the role of m6A RNA methylation regulators in progression and prognosis of adrenocortical carcinoma. (aging-us.com)

Numbers shown below represent number of publications held in OncomiRDB database for Oncogenic and Tumor-Suppressive MicroRNAs. (cancerindex.org)
MiR-125b participates in the oncogenic role of lncRNA MALAT1 in the phenotypes including cell proliferative and invasive rates in laryngocarcinoma [ 6 ]. (hindawi.com)
The oncogenic communication with tumor cells and through the crosstalk of autocrine and paracrine components in almost all tumor types are responsible for this phenomenon. (biomedcentral.com)
While there is some controversy surrounding recent research that claims that miR-155 may display anti-oncogenic or pro-immunological benefits (Cell Rep 2:1697-1709, 2012), most research seems to point to the importance of anti-miRs, with anti-miR-155 in particular, for cancer therapy. (biomedcentral.com)
AP4 (TFAP4) encodes a basic helix-loop-helix leucine zipper (bHLH-LZ) transcription factor and is a direct target gene of the oncogenic transcription factor c-MYC. (biomedcentral.com)
Unfortunately, some targets with potent oncogenic activity fall into this category, and currently little is known about how to solve this problem, which largely hampered drug research on human cancers. (techscience.com)

The invention comprises "methods and compositions for the diagnosis, prognosis, and treatment of hepatocellular carcinoma … [as well as] methods of identifying [anti-cancer] agents," the patent's abstract states. (genomeweb.com)
Therefore, understanding the pathogenesis of cancer, identifying biomarkers that aid in the early diagnosis of digestive tract tumors, and finding therapeutic targets are of great significance in reducing the incidence of digestive tract tumors and improving prognosis. (ijbs.com)
Abstract Circulating tumor cells (CTCs) are essential biomarkers for liquid biopsies, which are important in the early screening, prognosis, and real-time monitoring of cancer. (techscience.com)

Further, high NEK2 expression promoted proliferation, colony formation, migration and invasion of HCC cell lines. (oncotarget.com)
TRIM13 inhibited cell proliferation and induced cell apoptosis by regulating NF-κB pathway in non-small-cell lung carcinoma cells. (cancerindex.org)
Overexpression of TRIM13 in NCI-H1975 and SPC-A-1 cells hampered cell proliferation. (cancerindex.org)
CCK-8, plate colony formation and EdU assays were performed to assess the cell proliferation. (biomedcentral.com)
In addition, SNHG4 or RRM2 knockdown significantly induced cell cycle arrest and cell senescence, and inhibited DNA damage repair and cell proliferation, and the effects can be partially reversed by let-7a knockdown or RRM2 reoverexpression. (biomedcentral.com)
Sorafenib (Sor) can block receptor tyrosine kinases, which are involved in the development of new blood vessels, as well as cell proliferation as a result [ 22 ]. (biomedcentral.com)
miR-597-3p could reduce the proliferation, invasion and migration abilities of SW579 cells, which may be related to the targeted inhibition of RAB23 expression and down-regulation of the expression levels of MMP-2, MMP-9 and N-cadherin proteins of SW579 cells. (viamedica.pl)
Knockdown of CASC9 inhibited the proliferation and invasion of OS cells. (biomedcentral.com)
Osteosarcoma (OS) is characterized by the proliferation of malignant tumor cells that can directly differentiate into bone or bone-like tissue [ 1 ]. (biomedcentral.com)
Then, we investigated the expression of CASC9 in OC cell lines and detected the influences of CASC9 silencing on cell proliferation, and invasion in OS cells and in mouse model. (biomedcentral.com)
HNRNPC was proven to promote the proliferation, migration and invasion of H295R and SW13 cells through MTT and Transwell assays. (aging-us.com)
MiR-155 also upregulates Mxd1/Mad1, a network of basic helix-loop-helix leucine zipper transcription factors which mediate cellular proliferation, differentiation, and apoptosis, through regulating BCL6. (biomedcentral.com)

The mature miRNA is incorporated into a RNA-induced silencing complex (RISC), which recognizes target mRNAs through imperfect base pairing with the miRNA and most commonly results in translational inhibition or destabilization of the target mRNA. (wikipedia.org)
A first RNA molecule comprises a double-stranded sequence, which includes a guide strand sequence that is complementary to a portion of an mRNA transcript encoded by the target gene. (genomeweb.com)
Preferably, the second guide strand sequence comprises one or more bases that are mismatched with a nucleic acid sequence of the mRNA transcript encoded by the target gene. (genomeweb.com)
The invention, the patent's abstract states, comprises "methods for identifying a polynucleotide that can reduce the level of a target mRNA. (genomeweb.com)
A reduction in secreted reporter protein activity relative to a control cell into which the test polynucleotide has not been introduced is indicative that the test polynucleotide is capable of reducing the level of the target mRNA in the cells. (genomeweb.com)
The method is adaptable for high-throughput screening methods and is suited for identifying polynucleotides that can catalyze cleavage of target mRNA and/or act on target mRNA through and antisense or RNAi mechanism. (genomeweb.com)
For the mRNA analysis, RNA (1 mg) was reverse-transcribed by using the PrimeScript RT reagent Kit with gDNA Eraser (Tiangen), the mRNA level of the AK4 gene was quantified using duplex-qRT-PCR analysis where the Taqman probes with different fluorescence for β-actin were used in the FTC-3000P. (biomedcentral.com)
We found that TRIM13 mRNA and protein expression was reduced in NSCLC tissues and cell lines in comparison to paired non-cancerous tissues and a human normal bronchial epithelial cell line, respectively. (cancerindex.org)
M6A RNA methylation can appear in 0.1% to 0.4% of total adenosine residues [ 9 ], and involves the mRNA regulation of more than 7600 genes [ 10 ]. (aging-us.com)
Therefore, m6A RNA methylation is the major method of posttranscriptional regulation and contributes to the regulation of mRNA stability, alternative splicing, intracellular distribution and transcription [ 11 ]. (aging-us.com)

Our study might provide novel biomarkers and potential therapeutic targets for OS treatment. (biomedcentral.com)
Li XP, Qu J, Teng XQ, Zhuang HH, Dai YH, Yang Z, Qu Q. The Emerging Role of Super-enhancers as Therapeutic Targets in The Digestive System Tumors. (ijbs.com)

Such methods generally comprise providing a cell with one or more precursor nucleic acid sequences that encode two or more RNA molecules. (genomeweb.com)
The term "oncotarget" encompasses all molecules, pathways, cellular functions, cell types, and even tissues that can be viewed as targets relevant to cancer as well as other diseases. (oncotarget.com)
NcRNAs are a class of RNA molecules that do not encode proteins, and they play an important role in regulating cellular transcription and post-transcriptional processes [ 7 ]. (ijbs.com)
Cell-mediated immune responses are initiated by T lymphocytes that are themselves stimulated by cognate peptides bound to MHC molecules on antig en-presenting cells (APC). (thermofisher.com)
Abstract The term "undruggable" is to describe molecules that are not targetable or at least hard to target pharmacologically. (techscience.com)
Ras , as one of the most common oncogenes, was previously considered "undruggable", but in recent years, a few small molecules like Sotorasib (AMG-510) have emerged and proved their targeted anti-cancer effects. (techscience.com)

Tripartite Motif Containing 13 (TRIM13), a member of TRIM proteins, is deleted in multiple tumor types, especially in B-cell chronic lymphocytic leukemia and multiple myeloma. (cancerindex.org)
Samples are mixed with a cocktail of biotinylated detection antibodies (Step 1) and then incubated with the array membrane which is spotted in duplicate with capture antibodies to specific target proteins (Step 2) . (rndsystems.com)
For further study, the Luciferase reporter assay proved that miR-597-3p could target the expression of RAB23, and the proteins of invasion and migration were also measured to clear the mechanism. (viamedica.pl)

Immunoflourescence images of expression of Nrf2 and HO-1 showing thealteration of key protein markers of antioxidant gene expression, read more in 'Melatonin targets ferroptosis through bimodal alteration of redox environment and cellular pathways in NAFLD model' from Saha et al, in this edition of Bioscience Reports. (portlandpress.com)
NcRNAs play a significant role in regulating gene expression in digestive system tumors. (ijbs.com)

In general, microRNAs, a class of small (~21 nucleotide) non-coding RNAs, negatively regulate the expression of their target genes. (spandidos-publications.com)
The multistep transformation of normal cells into malignant cells involves genetic and epigenetic alterations that promote the aberrant expression of critical oncogenes and tumor suppressor genes [ 1 , 2 ]. (ijbs.com)
To further confirm, the expression of candidate genes was investigated in exosomes derived from the parental HT-29 colorectal cancer cell line (HT-29-EXOs), and cancer stem cells (CSCs) -enriched spheroids (CSC-EXOs) derived thereof. (researchsquare.com)

TRIM13-induced NSCLC cell apoptosis was attenuated by a caspase-3 inhibitor Ac-DEVD-CHO, suggesting that TRIM13 induced cell apoptosis partially through a caspase-3-dependent pathway. (cancerindex.org)
We reported novel notch signaling inhibitor(s), and apoptosis inducer agents in cancer and cancer stem-like cells. (edu.in)
Therefore, the current study used integrated in vitro and in silico approaches to figure out Amy and Sor's possible synergistic activity in targeting AMPK/mTOR and BCL-2 for anti-angiogenesis and apoptosis cell death in HepG2 cells. (biomedcentral.com)
A substantial synergistic interaction between Amy and Sor was observed (CI 50 = 0.56), which was connected to cell cycle arrest at the S and G2/M stages and increased apoptosis and potential necroptosis. (biomedcentral.com)
This inhibition of AMPK ultimately leads to inhibition of mTOR and thus induces apoptosis in the HepG2 cells. (biomedcentral.com)
They showed that wild-type MaSCs and basal cells are more resistant to apoptosis and exhibit increased non-homologous end joining activity. (stemcellsciencenews.com)

These findings suggest miR-486-5p negatively regulates NEK2, which is a critical prognostic indicator of HCC patient survival after liver transplantation. (oncotarget.com)
The AK4 gene is one of the targets of miR-199a-3p and negatively correlates with the effect of miR-199a-3p on OS drug-resistance. (biomedcentral.com)
Furthermore, the expression of BANCR was negatively regulated by miR-153-3p and identified as a target gene of miR-153-3p using luciferase reporter assays. (molcells.org)

The pathology revealed a malignant neoplasm composed of tubules, papillary structures, ribbons of primitive stratified columnar cells, vesicular nuclei, and high nuclear-cytoplasmic ratio. (biomedcentral.com)
The most common malignant tumor, hepatocellular carcinoma (HCC), arises from well-differentiated hepatocytes [ 1 ]. (biomedcentral.com)
Aurora A overexpression in many malignant tumor types suggests that it may be directly involved in NEOPLASTIC CELL TRANSFORMATION. (lookformedical.com)
Adrenocortical carcinoma (ACC) is a rare malignant tumor with an incidence rate of approximately 0.7/million to 2.0/million in the population [ 1 ]. (aging-us.com)

SEs are defined as large clusters of active enhancers in large clusters that are enriched with high levels of transcription factors (TFs), master co-factors, mediator complexes, RNA polymerase II (RNA pol II), and epigenetic modifications [ 5 , 7 ]. (ijbs.com)

We found that, due to the hypermethylation of its CpG island, miR-148a undergoes methylation-mediated silencing in HCC cell lines. (spandidos-publications.com)
Additionally, DNMT1, the DNA methyltransferase that maintains methylation patterns, is aberrantly upregulated in HCC cell lines, and its overexpression is responsible for hypermethylation of the miR-148a promoter. (spandidos-publications.com)

In this study, using a systematic analysis in the multi-drug sensitive (G-292 and U2OS) and resistant (MNNG/HOS) OS cell lines, we found that miR-199a-3p inhibits multi-drug resistance of OS. (biomedcentral.com)
Overexpression of long intergenic noncoding RNA LINC00312 inhibits the invasion and migration of thyroid cancer cells by down-regulating microRNA-197-3p. (viamedica.pl)
It is reported that J43 inhibits the binding of mouse PD-L1-Ig and mouse PD-L2-Ig to PD-1/BHK transfected cells. (thermofisher.com)
For example, a small peptide that is encoded by lncRNA HOXB-AS3 inhibits oncogenesis by regulating alternative splicing and metabolic reprogramming of colon cancer cells [5-7, 17]. (fortunepublish.com)

This study aimed to elucidate the role of miR-153-3p-EVs in the invasion and migration capabilities of LUAD cells and explore its mechanism through in vivo and in vitro experiments. (molcells.org)
Since then, several microproteins have been functionally characterized, which may act, for example, as signals promoting cell migration and differentiation of human cells [5, 7]. (fortunepublish.com)

The invention comprises "a method of diagnosing, assessing susceptibility, and/or treating schizophrenia involving the identification and/or observation of microRNAs and variant miRNA," according to the patent's abstract. (genomeweb.com)
microRNA alleles associated with schizophrenia and schizophrenia spectrum disorders were identified and ultra-rare variants in the precursor or mature miRNA were identified. (genomeweb.com)
Effect of Withania somnifera phytoconstituents on miRNA expression profile in breast cells: Therapeutic and Cytoprotective role. (edu.in)
The miR mimics or inhibitor was transfected into laryngocarcinoma cell lines M4E and Hep2 for the investigation of the biological functions (proliferative, invasion, migratory rates, and apoptotic rates) of this miRNA. (hindawi.com)
Bioinformatic analysis as well as experimental validation suggested that SNHG4 regulates RRM2 expression via a let-7 miRNA-mediated ceRNA network. (biomedcentral.com)
We have identified anti-cancer effects of Huaier, and its unique characteristics of 1) no side effects or toxicity, 2) in dose dependent manner, 3) causing specific cancer cell death with simultaneous tissue regeneration, 4) miRNA-mediated transcriptional control on the rescue of multiple signal transduction, by dependent on individual genomic potential. (fortunejournals.com)

Other lncRNAs are localized in the nucleus, which are involved in crucial molecular processes, including RNA processing, chromatin organization and transcription, and play an important role in genome structure [ 13 , 14 ]. (biomedcentral.com)

We performed the qRT-PCR, western blot and the luciferase reporter assays to test whether Adenylate Kinase 4 (AK4) is the target of miR-199a-3p. (biomedcentral.com)
Ectopic kinase overexpression in any culture cell line leads to polyploidy and centrosome amplification. (lookformedical.com)
Those transformed cells form tumours when implanted in immunodeficient mice, indicating that the kinase is an oncogene. (lookformedical.com)
DUSP6/MKP3 is a dual-specific phosphatase that regulates extracellular regulated kinase ERK1/2 and ERK5 activity, with an increasingly recognized role as tumor suppressor. (cancer-genetics.org)

Advancements in RNA-based technology have improved understanding of the complexity and diversity of the TIME and its effects on therapy. (biomedcentral.com)
The complex interplay between cancer cells and the TIME influences the outcome of immunotherapy and other anticancer therapy (Fig. 1 ). (biomedcentral.com)
During the last decade, there have been major breakthroughs in many aspects of diagnosing and treating NSCLC, including surgery, chemotherapy, radiation therapy, and targeted drug therapy. (jcancer.org)

Our data revealed that both miR-199a-3p and its target gene AK4 are reversely correlated with the OS drug resistance. (biomedcentral.com)
In addition, amino acid metabolism plays an important role in regulating tumor immunity and targeting amino acid metabolism may help to overcome immunotherapy resistance and improve existing therapies for COAD patients. (frontiersin.org)
Hence, it is of great importance to investigate the mechanisms that induce enzalutamide resistance in prostate cancer cells. (biomedcentral.com)
In vitro and in vivo experiments showed that SNHG4 overexpression markedly enhanced cell resistance to enzalutamide. (biomedcentral.com)
Cancer cells frequently display intrinsic or acquired resistance to chemically diverse anticancer drugs, limiting therapeutic success. (nature.com)
Biedler, J. L. & Riehm, H. Cellular resistance to actinomycin D in Chinese hamster cells in vitro: cross-resistance, radioautographic, and cytogenetic studies. (nature.com)
The SEs consist of critical epigenetic regulatory elements, which modulate the biological characteristics of digestive system tumors including tumor cell identity and differentiation, tumorigenesis, environmental response, immune response, and chemotherapeutic resistance. (ijbs.com)
AP4 -deficiency also sensitized CRC cells to 5-FU treatment, whereas ectopic AP4 conferred resistance to 5-FU in a miR-22-3p and MDC1-dependent manner. (biomedcentral.com)

Low molecular weight heparin (LMWH), the guideline based drug for prophylaxis and treatment of cancer-associated thrombosis, was recently shown to sensitize cisplatin resistant A2780cis human ovarian cancer cells for cisplatin cytotoxicity upon 24 h pretreatment with 50 μg × mL -1 of the LMWH tinzaparin in vitro , equivalent to a therapeutic dosage. (oncotarget.com)
Here we aim to illuminate the underlying molecular mechanisms of LMWH in sensitizing A2780cis cells for cisplatin. (oncotarget.com)
My laboratory is interested in targeting metabolic and molecular pathways in cancer and cancer stem cells by using preclinical cancer models and computer-aided drug discovery approach. (edu.in)
To study the molecular mechanism of natural compounds as Notch Signaling inhibitor in cancer cell lines: in silico and in vitro investigation (completed). (edu.in)
Scientists investigated the molecular and physiological consequences of DNA damage in wild-type mammary stem cells (MaSCs), p53-null MaSCs, and p53-null TICs. (stemcellsciencenews.com)

Thereby, LMWH induced sensitization by transcriptional reprogramming of A2780cis cells via not yet elucidated mechanisms that depend on cellular proteoglycans. (oncotarget.com)
However, there are still limitations in the treatment and diagnosis of PAAD, and further research to explore new mechanisms and discover new potential targets is still warranted. (hindawi.com)

Extracellular vesicles (EVs) play an essential role in the communication between cells and the tumor micro-environment. (molcells.org)
Extracellular vesicles (EVs) are vesicular bodies of lipid membranes secreted and released by various types of cells. (molcells.org)

Investigators evaluated the effects of PEGylated interferon-α2a and 5-fluorouracil on the expression of cancer stem cell (CSC) markers and on specific pathways that contribute to the propagation of CSCs in hepatocellular carcinoma. (stemcellsciencenews.com)
The results revealed significant differences in the behavior and coordination of RNA editing events, biomolecules, and multiple signaling pathways which beyond any speculations from the findings in the process of carcinogenesis. (fortunejournals.com)

Tumor-infiltrating immune cells are an important component of the TIME and are a significant predictor of cancer patients' survival. (biomedcentral.com)
Previous study revealed that the protective effect of TIGAR in cell survival is mediated through the increase in PPP (pentose phosphate pathway) flux. (nature.com)
The Cdk5-AMT signal pathway involved in regulation of DDR by TIGAR defines a new role of TIGAR in cancer cell survival and it suggests that TIGAR may be a therapeutic target for cancers. (nature.com)
We have previously reported that TIGAR plays a pro-survival role in cancer cells through increase PPP flux 19 . (nature.com)
The microenvironment of the cell and the interactions of many growth factors have a role in tumor development and survival [ 2 , 7 ]. (biomedcentral.com)

Results of cellular internalization further confirmed better uptake of hyaluronic acid engineered nano-micelles in triple-marker positive pancreatic cancer stem-like cells compared with triple-marker negative counterparts. (stemcellsciencenews.com)
Tumor-derived EV modulates cellular activities in recipient cells by transferring genetic information from cancer cells. (researchsquare.com)
Inactivation of AP4 in CRC cell lines resulted in increased spontaneous and c-MYC-induced DNA damage, chromosomal instability (CIN) and cellular senescence. (biomedcentral.com)

Tumor xenograft data from Balb/c nude mice demonstrated that HCC cells with high NEK2 expression formed larger tumors than those with low NEK2 expression. (oncotarget.com)
The core transcription regulatory loop of the digestive system tumors is complex and a high density of transcription regulatory complexes in the SEs and the crosstalk between SEs and the noncoding RNAs. (ijbs.com)
Digestive system tumors including esophageal cancer, colorectal cancer, gastric cancer, pancreatic cancer, hepatic cancer, cholangiocarcinoma, and gallbladder carcinoma are associated with significantly high mortality and morbidity rates [ 1 ]. (ijbs.com)

The authors review and summarize advantages and limitations of tumor transplantation and lineage-tracing assays in support of a combinatorial approach to accurately define the roles of both cancer-initiating and cancer-propagating cells. (stemcellsciencenews.com)
Colony formation, β-gal staining, immunofluorescence, comet and homologous recombination (HR) assays and RNA-Seq analysis were used to determine the effects of AP4 inactivation. (biomedcentral.com)
Immunofluorescence, chromosome and micronuclei enumeration, MTT and colony formation assays were used to determine the effects of AP4 inactivation and target gene regulation on chromosomal instability (CIN) and drug sensitivity. (biomedcentral.com)

Primary liver cancer mainly refers to hepatocellular carcinoma (HCC), cholangiocarcinoma and hepatic angiosarcoma. (spandidos-publications.com)
We analyzed the relationship between differential expression of NEK2 and hepatocellular carcinoma (HCC) patient outcomes after liver transplants. (oncotarget.com)

The RNA-sequencing-based transcriptome for 524 patients with COAD from The Cancer Genome Atlas (TCGA) was selected as a training set. (frontiersin.org)

Hepatocellular carcinoma (HCC) is the fifth most common cancer and the third leading cause of cancer-related death worldwide, with more than 780,000 new cases and around 745,000 deaths each year [ 1 ]. (oncotarget.com)
For better management of the disease, we are trying to identify putative prognostic, diagnostic, and therapeutic biomarkers associated with cancer and cancer stemness at the gene and RNA (coding and non-coding) level. (edu.in)
The miR-29a-3p level was decreased, and PTEN level was elevated in laryngocarcinoma tissues and the cancer cell lines. (hindawi.com)
In this review, we discuss the available RNA-based cancer immunotherapies targeting the TIME. (biomedcentral.com)
More importantly, we summarize the potential of various RNA-based therapeutics clinically available for cancer treatment. (biomedcentral.com)
RNA-dependent targeting of the TIME, as monotherapy or combined with other evolving therapeutics, might be beneficial for cancer patients' treatment in the near future. (biomedcentral.com)
Therefore, understanding the TME and its immune cell components are equally important as cancer cell characteristics for tumor eradication. (biomedcentral.com)
During the tumor initiation stage, cancer cells escape from immune surveillance. (biomedcentral.com)
Results showed that TIGAR was increased and relocated to the nucleus after epirubicin or hypoxia treatment in cancer cells. (nature.com)
MYL9: a New Member of Myosin Motors, a Key Driver of Cancer Cell Dynamic Regulation? (cusabio.com)
By disrupting the interaction between MYL9 and CD69, researchers found that it can potentially boost the anti-tumor immune response, make MYL9 an attractive target for cancer immunotherapy [2] . (cusabio.com)
Considering its association with the myosin motors family, MYL9 holds promise as a novel drug target for cancer immunotherapy! (cusabio.com)
They demonstrated that miR-214 overexpression enhances stem-like properties in lung adenocarcinoma (LAC) cells and that miR-214 shows increased expression in cancer stem-like cells derived from primary tumor tissue and from two LAC cell lines. (stemcellsciencenews.com)
The anticancer activity of 3, 4-difluorobenzylidene curcumin loaded nano-micelles against MiaPaCa-2 and AsPC-1 human pancreatic cancer cells revealed dose-dependent cell killing. (stemcellsciencenews.com)
The authors investigated the potential of curcumin to reduce breast cancer stem cell (BCSC) population for sensitizing breast cancer cells to mitomycin C both in vitro and in vivo . (stemcellsciencenews.com)
Cancer stem-like cells and nonstem-like cancer cells were co-cultured, and defined cell types were chosen and irradiated, respectively, with proton microbeam. (stemcellsciencenews.com)
Visit our reviews page to see a complete list of reviews in the cancer stem cell research field. (stemcellsciencenews.com)
MEGA-DATA analysis in the process of cancer recovery by total RNA and small non-coding RNA sequencing. (fortunejournals.com)
Meanwhile, current investigations show that RAB23 also plays an important role in cancer cell-related processes. (viamedica.pl)
Cancer cells depart their normal microenvironment from the primary tumor site through complicated and multistep processes disseminate and colonize distant organs [ 1 ]. (intechopen.com)
High-mobility group protein box 1 (HMGB1) is overexpressed and reported to be a prognostic factor in patients with non-small-cell lung cancer (NSCLC). (jcancer.org)
The microRNA miR-155 is prominent in cancer biology. (biomedcentral.com)
Among microRNAs that have been linked to cancer, it is the most commonly overexpressed in malignancies (PNAS 109:20047-20052, 2012). (biomedcentral.com)
As such, targeted therapies may prove beneficial to cancer treatment. (biomedcentral.com)
Pancreatic cancer is the fourth leading cause of cancer-related death in the USA and Europe [ 1 ], and it is expected to overtake lung carcinoma as the second leading cause by 2030 [ 2 ]. (hindawi.com)
Here, we set out to determine the relevance of AP4 in human colorectal cancer (CRC) cells. (biomedcentral.com)
Role of Dusp6 Phosphatase as a Tumor Suppressor in Non-Small Cell Lung Cancer. (cancer-genetics.org)

In contrast, the knockdown of CTNNB1 and overexpression of miR-194, but not miR-192, in LKO hepatocytes and AML12 cells increased CYP7A1 expression. (bvsalud.org)
However, overexpression of aurora-A in particular cell lines such as NIH3T3 is sufficient to induce growth on soft agar. (lookformedical.com)

Further studies with pharmacological and genetic approaches revealed that TIGAR regulated the phosphorylation of ATM, a key protein in DDR, through Cdk5. (nature.com)
We report a case of PME with an interesting target protein expression suggesting a possible alternative therapeutic strategy for this rare tumor. (biomedcentral.com)
The neoplastic cells showed a diffuse positivity for CD56 and WT1 (cytoplasmic) and a variable positivity for NSE, Synaptophisin, S100 protein and Cytokeratin MNF116, while they were negative for CD99, alpha-fetoprotein, CD30, OCT3/4, β-HCG. (biomedcentral.com)
HE 4 × (A) . Positivity of the neoplastic cells for S100 protein 60× (B) and for PanCytokeratin 60× (C) . (biomedcentral.com)
While decreased expression of the distal 3'UTR of NAT12/NAA30 was generally observed in glioblastoma-initiating cells (GICs) and glioblastomaa (GBMs), this gene was strongly up-regulated at the protein level in GBM and GICs. (stemcellsciencenews.com)
The protein encoded by this gene can bind to a functionally important site in the T-cell receptor-alpha enhancer, thereby conferring maximal enhancer activity. (cancer-genetics.org)

We found MGO can induce mitochondrial ROS production and mitochondrial membrane potential loss, but reduce cytosolic ROS level in ARPE-19 cells. (bvsalud.org)
Epirubicin, a DNA damaging anticancer agent and CoCl 2 , which was used to imitate hypoxia condition, was applied to induce DNA damage in TIGAR knockdown HepG2 cells. (nature.com)

Metabolic reprogramming is a common feature of tumor cells, which is crucial for rapid tumor growth and adaption to tumor microenvironment ( 5 , 6 ). (frontiersin.org)
Targeting tumor cells or the tumor microenvironment (TME) are the two major fundamental principles for antitumor therapies. (biomedcentral.com)
Moreover, the MYL9-CD69 system depletes effector T cells in the tumor microenvironment, weakening the anti-tumor immune response. (cusabio.com)

Luciferase reporter assay and RNA immunoprecipitation assay were performed to verify the RNA-RNA interactions. (biomedcentral.com)

The patent, its abstract states, relates to "duplex oligonucleotide complexes [that] can be administered to a cell, tissue or organism to silence a target gene without the aid of a transfection reagent. (genomeweb.com)
The duplex oligonucleotide complexes of the disclosure include a conjugate moiety that facilitates delivery to a cell, tissue, or organism. (genomeweb.com)
Besides, we found that miR-153-3p-EVs could damage the respiratory ability of mice and produce a mass of inflammatory cells around the lung tissue of mice. (molcells.org)

and introducing at least one siRNA that is complementary to the siRNA tag of the transfected genetic construct to inhibit the expression of the target gene. (genomeweb.com)
MiR mimics could inhibit proliferative, invasive migratory rates while promoting apoptotic rates of M4E and Hep2 cells. (hindawi.com)
Also, since BCL6 interacts with several co-repressor complexes to inhibit transcription, and its gene is frequently trans-located and hyper-mutated in diffuse large B cell lymphoma (DLBCL), miR-155 acts to enhance transcription and contribute to the pathogenesis of DLBCL. (biomedcentral.com)

This transcription factor is involved in the Wnt signaling pathway, and it may function in hair cell differentiation and follicle morphogenesis. (cancer-genetics.org)

So far, targeted therapies and immunotherapy have shown effectiveness only in a limited subset of CRC patients. (biomedcentral.com)

EMT is an embryologically conserved genetic program by which epithelial cells down regulate intercellular tight junctions, loose polarity, express mesenchymal markers, and manifest a migratory phenotype [ 1 ]. (intechopen.com)

TIME-related RNA or RNA regulators could be promising targets for anticancer immunotherapy. (biomedcentral.com)

DNA damage in HepG2 cells after TIGAR knockdown with or without treatment of epirubicin or CoCl 2 was detected by Comet assay. (nature.com)

methods for reducing the expression level of a target gene. (genomeweb.com)
The invention relates to a method for inhibiting the expression of a target gene in a eukaryotic cell," the patent's abstract states. (genomeweb.com)
Intriguingly, the expression of DNMT1, which is a target of miR-148a, is inversely correlated with the expression of miR-148a in HCC cells. (spandidos-publications.com)
We also studied the microRNAs that affect NEK2 expression. (oncotarget.com)
Hence, LMWH reduces the cisplatin-induced rise in Wnt activity and TCF-4 expression in A2780cis cells, but keeps sensitive A2780 cells unaffected. (oncotarget.com)
The present study explored the expression and potential role of TRIM13 in non-small-cell lung carcinoma (NSCLC). (cancerindex.org)
To define a role of TIGAR in DNA damage, TIGAR expression was knocked down with siRNA in HepG2 cells. (nature.com)
Abnormal expression of MYL9, which is associated with cell motility. (cusabio.com)
The expression of matrix metalloproteinase (MMP)-2, MMP-9, and N-cadherin was down-regulated, and the expression of E-cadherin was up-regulated. (viamedica.pl)
Evidence reported to date suggests overlapping functions for these ligands and their constitutive expression on some normal tissues and upregulation on activated antigen-presenting cells. (thermofisher.com)
A CRISPR/Cas9 approach was employed to generate AP4 -deficient CRC cell lines with inducible expression of c-MYC. (biomedcentral.com)
AP4 -deficient cells displayed increased expression of the long non-coding RNA MIR22HG, which encodes miR-22-3p and was directly repressed by AP4. (biomedcentral.com)
Furthermore, Mediator of DNA damage Checkpoint 1 ( MDC1 ), a central component of the DNA damage response and a known target of miR-22-3p, displayed decreased expression in AP4 -deficient cells. (biomedcentral.com)
Inhibition of miR-22-3p or ectopic MDC1 expression reversed the increased senescence, DNA damage, CIN and defective HR observed in AP4 -deficient CRC cells. (biomedcentral.com)

This study aimed to explore the biological role of long on-coding RNA (lncRNA) CASC9 and its regulatory mechanism in OC. (biomedcentral.com)

Herein, we describe the epigenetic regulation of miR-148a and its impact on HCC cells. (spandidos-publications.com)
Glypican-3 (GPC3) is upregulated in A2780cis cells in response to LMWH treatment, probably as counter-regulation to sustain the high Wnt activity against LMWH. (oncotarget.com)

The BIC gene is activated by promoter insertion at a retroviral integration site on chromosome 21q21 in B cell lymphomas induced by avian leukosis virus. (biomedcentral.com)

Our results suggest the existence of a novel miR-148a-DNMT1 regulatory circuit and indicate that miR-148a acts as a tumor suppressor during hepatocellular carcinogenesis. (spandidos-publications.com)
MYL9, the regulatory light chain of myosin, is essential for cell movement by activating myosin motility. (cusabio.com)

Using TCF/LEF luciferase promotor assay (Top/Flash) we show that resistant A2780cis cells possess a threefold higher Wnt signaling activity compared to A2780 cells. (oncotarget.com)
The miRTarase Database predicated and Luciferase reporter assay proved that RAB23 was the target gene of miR-597-3p. (viamedica.pl)

Anatomy11

Organisms7

Diseases53

Chemicals and Drugs92

Analytical, Diagnostic and Therapeutic Techniques and Equipment47

Phenomena and Processes45

Disciplines and Occupations1

Information Science5

Health Care12

MiRNAs24

Squamous cell carc4

Metastasis6

Progression13

Oncogenic6

Prognosis3

Proliferation12

MRNA10

Therapeutic Targets2

Molecules6

Proteins3

Gene expression2

Genes3

Apoptosis6

Negatively3

Malignant4

Polymerase1

Methylation2

Inhibits4

Migration2

MiRNA6

LncRNAs1

Kinase4

Therapy3

Resistance8

Molecular5

Mechanisms2

Extracellular2

Pathways2

Survival5

Cellular3

Tumors3

Assays3

Liver2

Transcriptome1

Cancer27

Overexpression2

Protein6

Induce2

Tumor Microenvironment3

Immunoprecipitation1

Tissue3

Inhibit3

Differentiation1

Therapies1

Intercellular1

Regulators1

Knockdown1

Expression14

Biological1

Regulation2

Chromosome1

Regulatory2

Luciferase2