Targeting of double stranded nucleic acid sequences peptide nucleic acids. Medical search. Frequent questions

To combat this triplex instability, nucleotide excision repair proteins (NERs) have been shown to recognize and repair triple-stranded DNA structures, reinstating full availability of the previously inhibited and unstable gene. (wikipedia.org)
Peptides derived from sequence of cell surface proteins, such as intercellular adhesion molecule-1 (ICAM-1), LHRH, Bombesin, and LFA-1, have shown potent binding affinity to the target cell surface receptors. (biosyn.com)
Arg-Gly-Asp (RGD) peptides,2 poly-Arg peptides), 3,4 proteins (e.g., antibodies,5 transport proteins, and transferrin6), and small molecules (e.g., folate7) have been used to selectively direct drugs to cancer cells with upregulated receptors by forming drug-carrier conjugates (Fig. 1). (biosyn.com)
A cell-penetrant peptide blocking C9ORF72-repeat RNA nuclear export reduces the neurotoxic effects of dipeptide repeat proteins. (le.ac.uk)
The present invention envisions phosphorylated forms of any one of these GEF-H1 peptides, polypeptides and proteins. (justia.com)
This technique has frequently been applied to animal tissue slices for the purpose to map proteins, peptides , lipids, sugars or small metabolites to find disease specific bio-markers or to study drag metabolism. (go.jp)
Click on the protein counts, or double click on taxonomic names to display all proteins containing PDZ domain in the selected taxonomic class. (embl.de)
In this work, we show that substrate recognition of Tsp is mediated by a PDZ domain, a small protein module that promotes protein-protein interactions by binding to internal or C-terminal sequences of their partner proteins. (embl.de)
Compared with traditional drugs targeting proteins, ASO presents various advantages, such as simplicity in design and higher specificity. (creative-biolabs.com)
In highly preferred embodiments, said polynucleotide sequences encode "stacked" Cry1F and Cry1Ac lepidopteran insect inhibitory proteins. (patsnap.com)
Tandem pore domain weak inward rectifying K+ (TWIK) channel nucleic acids and proteins that have been isolated from Drosophila melanogaster and Leptinotarsa are described. (patsnap.com)
The TWIK channel nucleic acids and proteins can be used to genetically modify metazoan invertebrate organisms, such as insects, coelomates, and pseudocoelomates, or cultured cells, resulting in TWIK channel expression or mis-expression. (patsnap.com)

Triple-stranded DNA (also known as H-DNA or Triplex-DNA) is a DNA structure in which three oligonucleotides wind around each other and form a triple helix. (wikipedia.org)
Peptide nucleic acids are synthetic oligonucleotides that resist protease degradation and are used to induce repair at site specific triplex formation regions on DNA genomic sites. (wikipedia.org)
Many oligo backbone modifications create unique binding capabilities and bolster sequence discrimination, making them useful for a variety of diagnostic and therapeutic applications such as SNP genotyping, antisense oligonucleotides, siRNA, and cell delivery. (biosearchtech.com)
Antisense oligonucleotides (ASOs) refer to synthetic chains of nucleotides targeting any genes of interest. (creative-biolabs.com)
Nucleic acid aptamers are single-stranded oligonucleotides (RNA or DNA) 30 to 100 nt long that can bind to and affect the activity of target molecules with high specificity and affinity. (creative-biolabs.com)

The approaches and results presented here will serve as a guide to systematically approach targeted delivery of cytotoxic drug molecules using peptides for treatment of several diseases. (biosyn.com)
They preferentially cleave single-stranded RNA molecules between purine and uridine residues to generate two nucleotide fragments with 2'3'-cyclic phosphate adenosine/guanosine terminus and uridine residue, respectively. (frontiersin.org)
Molecules containing both aromatic peptides and nucleic acids could in principle exploit different forces to self-assemble. (cnr.it)
Ribozymes are single-stranded RNA molecules that specifically inhibit viral gene expression and replication. (creative-biolabs.com)
There are three pathways capable of producing double-stranded RNA (dsRNA) molecules that activate RNAi, including microRNA (miRNA), short interfering RNA (siRNA), and short hairpin RNA (shRNA). (creative-biolabs.com)

Global Nucleic Acid Therapeutics CDMO Market to Reach $14.19 Billion by 2033. (gii.tw)
The global nucleic acid therapeutics CDMO market was valued at $3.88 billion in 2022 and is expected to reach $14.19 billion by 2033, growing at a CAGR of 12.55% during the forecast period 2023-2033. (gii.tw)
The key factors driving the growth of the global nucleic acid therapeutics CDMO market include the growing demand for nucleic acid therapeutics applications to treat chronic and genetic diseases, manufacturing advancement for producing nucleic acid by CDMO (a contract development and manufacturing organization), increasing FDA (Food and Drug Administration) approvals of nucleic acid therapeutics, and increasing investment for the expansion of CDMO manufacturing units. (gii.tw)
According to BIS Research , the nucleic acid therapeutics CDMO market refers to the products and services provided by CDMO companies for the manufacturing of nucleic acid therapeutics. (gii.tw)
Theoretically, nucleic acids-based antiviral therapeutics are dedicated to inhibiting the expression of viral or host factor genes via DNA or RNA. (creative-biolabs.com)

This entailed mining available transcriptomic and/or genomic sequence datasets for the presence of homologues of known TIMPs, predicting secondary structures of defined protein sequences, systematic phylogenetic analyses and assessment of differential expression of genes encoding putative TIMPs in the developmental stages of A. suum , N. americanus and Schistosoma haematobium which infect the mammalian hosts. (biomedcentral.com)
All targeted genome engineering relies on the introduction of a site-specific double-strand break (DSB) in a pre-determined genomic locus by a rare-cutting DNA endonuclease . (ac.ir)
The main consideration in the construction of genomic or cDNA libraries is, therefore, the nucleic acid starting material. (biologydiscussion.com)
This means that digesting genomic DNA with EcoRI, which recognizes the sequence 5′-GAATTC-3′, will produce fragments each of which is, on average, just over 4 kb. (biologydiscussion.com)
The assays are based on the DNA sequences of recombinant constructs inserted into the cotton genome and of the genomic sequences flanking the insertion sites. (patsnap.com)

In the structural modification of PNA, there are few types of base modification, mainly the introduction of monomers composed of pseudocytosine, in order to make the modified peptide nucleic acid and DNA form a triple helix without pH dependence. (creative-peptides.com)

Monomer for Me-phosphonate nucleic acid synthesis. (biosearchtech.com)
Synthesis resembles peptide synthesis. (biosearchtech.com)
Both linear and cyclic peptides have been explored as trafficking moiety due to ease of synthesis, structural simplicity, and low probability of undesirable immunogenicity. (biosyn.com)

Also disclosed are methods of identifying related polypeptides and polynucleotides, methods of making and using transgenic cells comprising the novel sequences of the invention, as well as methods for controlling an insect population, such as the Western Corn Rootworm and Colorado potato beetle, and for conferring to a plant population resistance to the target insect species. (patsnap.com)

TFOs can inhibit transcription by binding with high specificity to the DNA helix, thereby blocking the binding and function of transcription factors for particular sequences. (wikipedia.org)

In peptide nucleic acid (PNA), the sugar-phosphate backbone of DNA is replaced with a protein-like backbone. (wikipedia.org)
The PNA-DNA triplex are stable because PNAs consist of a neutrally charged pseudopeptide backbone which binds to bind to the double stranded DNA (dsDNA) sequence. (wikipedia.org)
Generate oligos with a neutral backbone that assists in binding DNA, RNA and double-stranded DNA. (biosearchtech.com)
For example, the inventors have identified the key amino acid of AAV1 responsible for enhanced in vivo transduction and selectively engineered this amino acid into the backbone of AAV2 and AAV3b. (justia.com)
in case of nucleic acids, Watson-Crick pairing drives self-assembly while, in case of peptides, backbone hydrogen bonds and interactions between aromatic side chains trigger the formation of structures, such as nanotubes or ribbons. (cnr.it)

Thus, while inculcating the UBPs in a DNA structure, their aromatic scaffolds must exhibit hydrophobic packing and must have hydrogen bond donor groups for the artificial nucleotides to interact with polymerases for their efficient incorporation in the non-template strand to bind with their UB partner in template strand [1-3]. (fortuneonline.org)

PNAs are able to bind with high affinity and sequence specificity to a complementary DNA sequence through Watson-Crick base pairing binding and are able to form triple helices through parallel orientation Hoogsteen bonds with the PNA facing the 5'-end of the DNA strand. (wikipedia.org)
These enzymes are the key to molecular cloning because of the specificity they have for particular DNA sequences. (biologydiscussion.com)

Multiregional whole-exome or genome sequencing has revealed that there is significant variation in the genetic makeup of tumor cells across different anatomical locations and within the same tumor over time ( 4 ). (spandidos-publications.com)
More specifically, this invention includes novel transformation events of cotton plants comprising one or more polynucleotide sequences, as described herein, inserted into specific site(s) within the genome of a cotton cell. (patsnap.com)

Figure 4: Amino acid sequence of ERα. (nature.com)
In a preferred embodiment, a GEF-H1 polypeptide is provided that lacks the amino acid region between residues 162 and 354 of SEQ ID NO. 2. (justia.com)
(A) Amino acid sequence of RNases T2 from human, mouse, rabbit, fruit fly, and classical swine fever virus showing the wide evolutionary conservation of these enzymes. (frontiersin.org)

The review also outlines the vast applications of UBPs as novel information storage components as well as in creation of semi-synthetic organisms expressing non canonical amino acids, high affinity aptamer generation, PCR based diagnostics and sitespecific labelling of RNAs. (fortuneonline.org)

Other restriction enzymes make staggered single-strand cuts, producing short single-stranded projections at each end of the digested DNA. (biologydiscussion.com)
Enzymes with 8 bp recognition sequences produce much longer fragments. (biologydiscussion.com)

Intramolecular triplex DNA is formed from a duplex with homopurine and homopyrimidine strands with mirror repeat symmetry. (wikipedia.org)
This intramolecular conformation is formed from the binding of the homopurine and purine strand of the duplex in an antiparallel fashion. (wikipedia.org)
TFOs bind specifically to homopurine-homopyrimidine regions that are often common in promoter and intron sequences of genes, influencing cell signaling. (wikipedia.org)
Similar to homopyrimidine in TFOs, homopyrimidine in PNAs are able to form a bond with the complementary homopurine in target sequence of the dsDNA. (wikipedia.org)

We therefore synthesized conjugates of the peptide FF to the peptide nucleic acid dimer "gc" and characterized their aggregates by different spectroscopic techniques, including NMR, CD and fluorescence. (cnr.it)

Based on these insert and border sequences, event-specific primers were generated. (patsnap.com)

Systematic analyses have revealed that physicochemical properties of the drug-peptide conjugates and their mechanism of receptor-mediated cellular internalization are important controlling factors for developing a successful targeting system. (biosyn.com)
This review is focused on understanding the factors involved in the development of an effective drug-peptide conjugate with an emphasis on the chemistry and biology of the conjugates. (biosyn.com)
Reported results on several promising drug-peptide conjugates have been critically evaluated. (biosyn.com)

Partial proteolysis by V8 protease at a single peptide bond immediately N-terminal to the PDZ domain resulted in two distinct and relatively stable fragments and complete loss of catalytic activity. (embl.de)

In triple-stranded DNA, the third strand binds to a B-form DNA (via Watson-Crick base-pairing) double helix by forming Hoogsteen base pairs or reversed Hoogsteen hydrogen bonds. (wikipedia.org)
The thymine hydrogen bonds with the adenosine (A) of the original double-stranded DNA to create a T-A*T base-triplet. (wikipedia.org)

Peptide binding of the ligand takes place in an elongated surface groove as an anti-parallel beta-strand interacts with the beta-B strand and the B helix. (embl.de)

Moreover, peptides derived from ICAM-1 receptor can be internalized by the leukemic T-cells along with the conjugated moiety offering the promise to selectively treat cancers and autoimmune diseases. (biosyn.com)

In this conformation, the homopyrimidine strand in the duplex bends back to bind to the purine strand in a parallel fashion. (wikipedia.org)
TFOs are short (≈15-25 nt) nucleic acid strands that bind in the major groove of double-stranded DNA to form intramolecular triplex DNA structures. (wikipedia.org)
Synthesise oligos that strongly and specifically bind to DNA, RNA and double-stranded DNA. (biosearchtech.com)
Using the new structure, the team tweaked some amino acids on the surface of the enzyme to make versions that could bind even better to the DNA where genetic integration occurs. (sbgrid.org)

A total of 15 protein sequences with high homology to known eukaryotic TIMPs were predicted from the complement of sequence data available for parasitic helminths and subjected to in-depth bioinformatic analyses. (biomedcentral.com)

Rapid immunoprecipitation mass spectrometry of endogenous protein (RIME) is a method that allows the study of protein complexes, in particular chromatin and transcription factor complexes, in a rapid and robust manner by mass spectrometry (MS). The method can be used in parallel with chromatin immunoprecipitation-sequencing (ChIP-seq) experiments to provide information on both the cistrome and interactome for a given protein. (nature.com)
Domain sequence analysis showed that the LcYTH protein had high sequence conservation. (bvsalud.org)
Photoaffinity labeling with a fluorescent nonpolar peptide caused the covalent attachment of the peptide to a single site on the Tsp protein. (embl.de)
The isolated PDZ domain (amino acids 206-334) is capable of folding into a well-behaved structure and binds to a nonpolar peptide with a dissociation constant (K(D)) of 1.9 microM, similar to that of the intact Tsp protein. (embl.de)
A beta-hairpin comprising the nuclear localization sequence sustains the self-associated states of nucleosome assembly protein 1. (colorado.edu)
Without the requirement of any protein, ribozymes can complex with the target sequences and hydrolyze the target site. (creative-biolabs.com)

MATERIAL AND METHODS: OSCC tissues and adjacent normal tissues were obtained to detect circRNAs expression by the next generation sequencing (NGS), and OSCC tissues were selected to verify the differentially significant circRNAs by reverse transcription-quantitative polymerase chain reaction (RT-qPCR). (bvsalud.org)
Bulk RNA sequencing (RNA-seq) data were obtained from inflamed and non-inflamed tissues from 170 patients with CD. (bvsalud.org)
The chimeric virus can also have a reduced transduction capability with respect to one or more cells or tissues (e.g., liver), which can be desirable in terms of targeting the vector to the target tissue of interest and reducing dosage of vector to be administered. (justia.com)
This enzyme is detected in all tissues, especially in embryonic tissues and immune cells ( https://www.proteinatlas.org/ ) ( Figures 1B,C ). The full-length human RNase T2 has 256 amino acids (AA) and a predicted size of 29 kD ( Table 1 ). (frontiersin.org)

Disclosed is a novel Lepidopteran- and Coleopteran-active delta-endotoxin polypeptide, and compositions comprising the polypeptide, peptide fragments thereof, and antibodies specific therefor. (patsnap.com)

We now offer several locked nucleic acid (LNA) phosphoramidites and CPGs, as well as methyl and ethyl phosphoramidites. (biosearchtech.com)

By using antibodies against the endogenous target, the cross-linked complex is immunoprecipitated, rigorously washed, and then digested into peptides while avoiding antibody contamination (on-bead digestion). (nature.com)

Systematic deletion mutagenesis of Tsp localized the binding site to amino acids 206-307, a region that completely encompasses the putative PDZ domain (217-301). (embl.de)

Peptides and nucleic acids can self-assemble to give supramolecular structures that find application in different fields, ranging from the delivery of drugs to the obtainment of materials endowed with optical properties. (cnr.it)

The structural and sequence features on pre-miRNA which can facilitate position and efficiency of cleavage are not well known. (biomedcentral.com)

Also disclosed are vectors, transformed host cells, and transgenic plants that comprise nucleic acid segments encoding the polypeptide. (patsnap.com)

Earlier studies systematically analyzed sequence variations in human pri-miRNAs/pre-miRNA and experimentally discovered that single nucleotide polymorphisms (SNPs) in miR-125a obstruct the processing of pri-miRNA to pre-miRNA [ 16 ]. (biomedcentral.com)

Accordingly, the present invention envisions an isolated nucleic acid encoding the GEF-H1S polypeptide of SEQ ID NO:2, as well as an isolated nucleic acid comprising the sequence of SEQ ID NO:1. (justia.com)
In this context, the invention also contemplates an isolated GEF-H1S polypeptide comprising the sequence of SEQ ID NO:2. (justia.com)
The invention also envisages a peptide consisting essentially of the sequence of SEQ ID NO. 3. (justia.com)

Comprehensive Transcriptomic Analysis of Novel Class I HDAC Proteolysis Targeting Chimeras (PROTACs). (le.ac.uk)

have also presented a TFO targeting the promoter sequence of bcl-2, a gene inhibiting apoptosis. (wikipedia.org)
Notch activator cyclopiazonic acid induces apoptosis in HL-60 cells through calcineurin activation. (go.jp)

Members of the institute have published in a wide range of peer-reviewed journals, including Nature, Molecular Cell, Angewandte Chemie, JACS, Science Advances, Proceedings of the National Academy of Sciences USA, Nucleic Acids Research, Chemical Science and many others. (le.ac.uk)

According to the National Centre for Biotechnology Information (NCBI), therapeutic nucleic acids (TNAs) are a subset of nucleic acids that are closely related compounds used to cure disease through the sequence-specific recognition of endogenous nucleic acids. (gii.tw)
10. Dreier B, Beerli RR, Segal DJ, Flippin JD, Barbas CF. Development of zinc finger domains for recognition of the 5'-ANN-3' family of DNA sequences and their use in the construction of artificial transcription factors. (ac.ir)
For example, the recognition sequence of 6 bp will occur, on average, every 4096 (4 6 ) bases, assuming a random sequence of each of the four bases. (biologydiscussion.com)

High-throughput sequencing methods are utilized to analyze the mutational spectrum and evolutionary trajectories of tumor cells. (spandidos-publications.com)

designed a sequence-specific TFO to the Ets2 promoter sequence that down-regulated the gene expression and led to a slowing of cell growth and cell death. (wikipedia.org)

Examples of triple-stranded DNA from natural sources with the necessary combination of base composition and structural elements have been described, for example in Satellite DNA. (wikipedia.org)

PDZ domains consist of 80 to 90 amino acids comprising six beta-strands (beta-A to beta-F) and two alpha-helices, A and B, compactly arranged in a globular structure. (embl.de)

With the advancement of next-generation sequencing and mass spectrometry, there is a growing need for the ability to merge biological features in order to study a system as a whole. (rsc.org)

Site-directed mutagenesis of a surface residue at the peptide binding site of the PDZ domain, valine 229, to Glu or Gln resulted in an increase in the K(M) value but had no effect on the k(cat) value. (embl.de)

As a research organization that provides full-service PNA services, Creative Peptides ' customer-centric collaborative approach helps us build true partnerships with customers. (creative-peptides.com)

Anatomy2

Organisms3

Diseases2

Chemicals and Drugs33

Analytical, Diagnostic and Therapeutic Techniques and Equipment15

Phenomena and Processes21

Anthropology, Education, Sociology and Social Phenomena1

Information Science10

Health Care2

Proteins12

Oligonucleotides5

Molecules5

Therapeutics5

Genomic5

Form a triple helix1

Synthesis3

Polypeptides1

Inhibit1

Backbone5

Aromatic1

Specificity2

Genome2

Amino Acid3

Synthetic1

ENZYMES2

Homopurine4

Dimer1

Primers1

Conjugates3

Protease1

Hydrogen bonds2

Helix1

Selectively1

Bind4

Homology1

Protein6

Tissues4

Fragments1

Ethyl1

Antibodies1

Systematic1

Structures1

Cleavage1

Vectors1

MiRNA1

Invention3

Analysis1

Apoptosis2

Advances1

Recognition3

Tumor1

Gene expression1

Structural1

Consist1

Next generation1

Binding site1

Approach1