Target nucleic acid using primers. Medical search. Frequent questions

Through the experiments on different toehold exchange probes with 5 correct targets and 55 spurious targets with energetically representative single-base changes (replacements, deletions, and insertions), Yin's group concluded that discrimination factors of these probes were between 3 and 100 + with the median 26. (wikipedia.org)
The probes function robustly from 10 °C to 37 °C, from 1 mM to 47 mM, and with nucleic acid concentrations from 1 nM to 5 M. They also figured out the toehold exchange probes work robustly even in RNA detection. (wikipedia.org)
Their design of molecular probes that target the genomic sequences of specific pathogens can enable diagnosticians and clinical laboratories to spot extremely small amounts of viral and bacterial pathogens in patients' biological samples, as well as in the environment and wildlife. (darkdaily.com)
HUB is a bioinformatics pipeline that designs probes for targeted DNA capture," according to their paper published in the journal Cell Reports Methods , titled, " Probe Design for Simultaneous, Targeted Capture of Diverse Metagenomic Targets . (darkdaily.com)
The probes' design makes possible simultaneous targeted capture of diverse metagenomics targets, Biocompare explained. (darkdaily.com)
ABSTRACT: Nucleic acid sequence based amplification (NASBA) is an isothermal nucleic acid amplification procedure based on target-specific primers and probes, and the co-ordinated activity of 3 enzymes: AMV reverse transcriptase, RNase H, and T7 RNA polymerase. (int-res.com)
Molecular beacons are hairpin shaped fluorescent oligonucleotide probes that can report the presence of a completely complimentary DNA target. (usda.gov)
Although acid fast smears of clinical specimens or histologic examination of tissues can detect mycobacteria, species identification requires either confirmation by culture or DNA probes. (vin.com)
Both amplification products are detected through fluorescent probes against the generated nucleic acids. (vin.com)
CDC scientists designed nucleic acid primers and probes which can be used in a sensitive test for detecting all known strains of Ebola virus (species Zaire ebolavirus ) including the 2014/2015 strain that emerged in West Africa and the more recent strain that caused an EVD outbreak in the Democratic Republic of Congo in 2017. (nih.gov)
The Luna Probe One-Step RT-qPCR Kit (No ROX) is optimized for real-time quantitation of target RNA sequences using hydrolysis probes. (neb.com)
The accuracy of T m calculations requires consideration of various physical properties of oligonucleotides, such as sequence length and composition, and accounts for experimental conditions, including the concentration of primers/probes, target oligo molecules, and salt/ionic strength. (idtdna.com)
Based on the knowledge and tools developed while identifying and characterizing new viruses and comparisons to previously-characterized viruses, new virus-specific and broad spectrum polyclonal and/or monoclonal antibody reagents, purification protocols, nucleic acid hybridization probes, PCR primers, isothermal amplification methods, and improved associated protocols will be developed. (usda.gov)
Specific primers and probes were designed and synthesized according to the gph gene sequence of Giardia and 18S rRNA gene sequence of Cryptosporidium. (bvsalud.org)

Nucleic acid (DNA and RNA) strands with corresponding sequences stick together in pairwise chains, zipping up like Velcro tumbled in a clothes dryer. (wikipedia.org)
The capture oligomers contain sequences complementary to specific regions of the target molecules as well as a string of deoxyadenosine residues. (cdc.gov)
Detection of the rRNA amplification product sequences is achieved using nucleic acid hybridization (HPA). (cdc.gov)
The (PCR) technique relies on primers that bind to nucleic acid sequences specific to an organism or group of organisms. (darkdaily.com)
Primers were designed to target sequences in the nodavirus capsid protein gene, yielding an amplification product of 120 nucleotides. (int-res.com)
Sequences diagnostic for E. chaffeensis from three different loci (16S rRNA, 120-kDa protein, and a variable-length polymerase chain reaction [PCR] target, or VLPT) were targeted for amplification by the PCR. (google.com)
Even though you have access to IDT's PrimeTime Predesigned qPCR Sequence Database, that contains predesigned qPCR sequences for human, mouse, and rat targets, we understand that sometimes a full customization of your PCR primers is needed. (idtdna.com)
It gives you the opportunity to use your own nucleic acid sequences to generate a list of primers that can be used in your custom designed experiments. (idtdna.com)
The present study aimed to establish a method for amplifying nearly full-length SSU rRNA gene sequences of previously unidentified prokaryotes, using newly designed targeted primers via primer evaluation in meta-transcriptomic datasets. (biomedcentral.com)
Primer binding regions of universal primer 8F/Arch21F for bacteria or archaea were used for primer evaluation of SSU rRNA sequences in meta-transcriptomic datasets. (biomedcentral.com)
Furthermore, targeted forward primers were designed based on SSU rRNA reads from unclassified groups unmatched with the universal primer 8F/Arch21F, and these primers were used to amplify nearly full-length special SSU rRNA gene sequences along with universal reverse primer 1492R. (biomedcentral.com)
Using this method, we identified unclassified SSU rRNA sequences that were not matched with universal primer 8F and Arch21F. (biomedcentral.com)
A new group within the Asgard superphylum was amplified by the newly designed specific primer based on these unclassified SSU rRNA sequences by using mudflat samples. (biomedcentral.com)
PCR targets had been chosen based mostly on nucleic acid sequences deposited in GenBank. (lcabie.com)
A primer is a strand of short nucleic acid sequences (generally about 10 base pairs) that serves as a starting point for DNA synthesis. (khanacademy.org)

In 2012, Yin's research group published a paper about optimizing the specificity of nucleic acid hybridization. (wikipedia.org)
With dye-based detection such as SYBR Green I, primer dimers also affect assay specificity because the nonspecific DNA-binding dye will bind to the primers and be detected along with the desired product. (sigmaaldrich.com)
To avoid strong 3'-terminal dimers while maintaining specificity, choose primers that have 2 G or C residues in the last 5 bases, 1 G or C in the last 3 bases and an A or T at the 3'-end ( Figure 9.1 ). (sigmaaldrich.com)
This increases the specificity for the target sequence. (usda.gov)
The demand for clinical signs or symptoms of pulmonary or sensitivity, specificity and speed of M. tu- extra-pulmonary TB) which had been sent berculosis detection led to the development to the TB laboratory at the Saudi-British of nucleic acid-based amplification tests to Laboratories for MTBC testing. (who.int)
Each lot of HotStarTaq Plus DNA Polymerase is subjected to a comprehensive range of quality control tests, including a stringent PCR specificity and reproducibility assay in which low-copy targets are amplified. (qiagen.com)
HotStarTaq Plus Master Mix Kit outperforms kits from other suppliers and ensures high specificity and superior performance in hot-start PCR (see figures " Highest specificity. Highest specificity. "> Highest specificity " and " Higher specificity with different primer-template systems. Higher specificity with different primer-template systems. "> Higher specificity with different primer-template systems ", and table). (qiagen.com)
The 5′-FAM probe (Eurogentec, Seraing, Belgium) contained locked nucleic acids (underlined) to improve specificity. (cdc.gov)
Proprietary assay design providing superior sensitivity and specificity for each target. (seqonce.com)

The discrimination factor Q is calculated as, the yield of correct target hybridization divided by the yield of spurious target hybridization. (wikipedia.org)
During the hybridization step, the sequence specific regions of the capture oligomers bind to specific regions of the target molecules. (cdc.gov)
Read this advice from our own thermodynamics specialist, Dr Richard Owczarzy, on the effects of probe and primer melting temperature (T m ) on hybridization. (idtdna.com)
Examples of techniques that include nucleic acid hybridization are northern and Southern analysis, PCR/qPCR, cloning, in situ hybridization, array analysis, gene knockdown , and next generation sequencing (NGS). (idtdna.com)
In this DECODED™ article, we learn from IDT scientist Dr Richard Owczarzy about oligonucleotide characteristics that impact melting temperature (T m ), a critical factor when planning experiments involving nucleic acid hybridization. (idtdna.com)
The criteria for hybridization are based on nucleic acid strand melting. (idtdna.com)
Important factors that affect T m , including oligo concentration, ion concentration, sequence mismatch, and target strand choice, can all play a signification role in hybridization experiments. (idtdna.com)

However, all assays have a set of optimal conditions and these are dependent on the instrument, selected reagents (buffer conditions), primer concentration and/or annealing temperature (T a ), magnesium concentration and even ramp rate. (sigmaaldrich.com)

Number represents nucleotide position of the first base in the target sequence (cRNA sense). (cdc.gov)
A nucleic acid test (NAT) is a technique used to detect a particular nucleic acid sequence and thus usually to detect and identify a particular species or subspecies of organism, often a virus or bacterium that acts as a pathogen in blood, tissue, urine, etc. (wikipedia.org)
The long primer strand has a corresponding (complementary) sequence to a "target" strand from the disease organism being detected. (wikipedia.org)
Complement is perfectly complementary with the target sequence. (wikipedia.org)
Primers are homologous to the desired target sequence. (sigmaaldrich.com)
When hybridized to the target sequence, the fluorophore and the quencher are separated and fluorescence is observed. (usda.gov)
A small target sequence within this gene is amplified (billions of copies are made) using PCR. (vin.com)
Such methods and systems of the invention can, e.g., be employed to identify (a) variation(s) in the nucleic acid sequence of a subject. (justia.com)
In DNA a sequence of three bases, which is called a codon, is responsible for the encoding of a single amino acid. (wikibooks.org)
IDT's easy-to-use online fusion primer design tool uses a gen-specific data base number or a supplied reference sequence to build appropriate 454 FusionPrimers for targeted DNA regions, including individual exons or all exons from one or more genes. (pressebox.com)
The software takes into account performance metrics of the entire fusion primer rather than simply adding 454 Life Sciences' universal adaptor sequence to an otherwise standard PCR primer. (pressebox.com)
The rpoB gene target sequence was amplified using mutagenesis forward and reverse primers followed by Hin dIII enzyme digestion. (who.int)
A total of 1,194 potential lactic acid bacterium (LAB) isolates were tentatively grouped and identified by repetitive element sequence-based PCR, followed by sequence-based identification using 16S rRNA and pheS genes from a selection of genotypically unique LAB isolates. (who.int)

single-stranded probe or primer molecules capture DNA or RNA target molecules of complementary strands. (wikipedia.org)
Target amplification assays are based on the ability of complementary oligonucleotide primers to specifically anneal and allow enzymatic amplification of the target nucleic acid strands. (cdc.gov)
Why do you have to cool the separated DNA strands to attach the primers? (khanacademy.org)
While folding of a single oligonucleotide is concentration independent, the T m is strongly influenced by oligo concentration when 2 or more nucleic acid strands interact. (idtdna.com)

Methods that directly detect bacteria from clinical specimens include acid fast and fluorescent smears, culture, and nucleic acid amplification such as polymerase chain reaction (PCR). (vin.com)

They are developing atomic-level models that explain how chemical energy is transduced into force and motion, and how dynamic assemblies control DNA replication, gene expression, chromosome superstructure, and other essential nucleic-acid transactions. (hopkinsmedicine.org)
At present, two amplification methods have been FDA approved for detection of M. tuberculosis from sputum in humans: the Amplicor system (Roche Molecular Systems, Branchburg, NJ) and the AMTD system (Gen-Probe Inc., San Diego, CA). The Amplicor system has as its target the mycobacterial 16S ribosomal RNA gene. (vin.com)
Because of small differences in the 16S ribosomal gene, the primers are specific to organisms only of the M. tuberculosis complex ( M. tuberculosis, M. bovis, M. africanum, M. microti ) and only these species are detected. (vin.com)
With our Single Primer Extension (SPE) chemistry at the core combined with Unique Molecular Indices (UMIs), you are ensured of complete target coverage for gene or fusion targets, enabling sensitive detection of known and novel variants down to 1% variant allele frequency. (qiagen.com)
Using the GenBank database ( www.ncbi.nlm.nih.gov ), we selected primers RcF (5′-GACGCTGCGTGAGTGATGA-3′) and RcR (5′-CCGGTGCTTCTTTACGCAGTA-3′), and probe RcS (5′-6 carboxyfluorescein-CTTTCGGGT T GTAAA A CTCTTTCGC G CA-Black Hole Quencher 1-3′), which amplify parts of the 16S rRNA encoding gene, to specifically amplify Rhabdochlamydia spp. (cdc.gov)
To enable quantification, a plasmid containing the target gene was constructed as described ( 4 , 9 ). (cdc.gov)
Analyze full-length infectious clones to determine the genes or gene products involved in replication, systemic movements, and pathogenicity to understand the role of viral pathogen genes in disease development and to identify new targets in the pathogen genome and tools for disease management. (usda.gov)
The minor sensitive gene target was RdRP. (bvsalud.org)

LAMP and RT-LAMP are commonly used isothermal amplification techniques that provides rapid detection of a target nucleic acid using LAMP-specific primers (supplied by the user) and a strand-displacing DNA polymerase. (neb.com)

The primer design can be reviewed with respect to the assay design guidance provided in PCR/qPCR/dPCR Assay Design . (sigmaaldrich.com)
Rapid, sensitive and precise probe-based qPCR detection and quantitation of target RNA targets. (neb.com)
Probe-based qPCR/RT-qPCR monitors an increase in fluorescence upon 5´ → 3´ exonuclease cleavage of a quenched, target-specific probe to measure DNA amplification at each cycle of a PCR. (neb.com)
Following nucleic acid extraction, the targets are amplified by real-time PCR (qPCR) and detected via fluorescent dye (FAM). (seqonce.com)
For example, in PCR/qPCR, the target concentration is usually designed to be much lower than that of the oligo probe. (idtdna.com)

We measured concentrations of SARS-CoV-2, influenza A and B virus, respiratory syncytial virus (RSV), mpox virus, human metapneumovirus, norovirus GII, and pepper mild mottle virus nucleic acids in wastewater solids at twelve wastewater treatment plants in Central California, USA. (nature.com)
Wastewater-based epidemiology uses concentrations of infectious disease targets in wastewater to understand disease occurrence in communities. (nature.com)
According to LabMedica , "A wide array of metagenomic study efforts are hampered by the same challenge: low concentrations of targets of interest combined with overwhelming amounts of background signal. (darkdaily.com)
The concentrations of the two targets were determined using real-time PCR technology. (bvsalud.org)

Currently, there are three basic types of tests to determine if an individual has been infected with SARS-CoV-2: viral nucleic acid (RNA) detection, viral antigen detection, and detection of antibodies to the virus. (medscape.com)
Viral tests (nucleic acid or antigen detection tests) are used to assess acute infection, whereas antibody tests provide evidence of prior infection with SARS-CoV-2. (medscape.com)
Compatible with DNA extraction methods designed to extract high quality DNA from bacterial, viral and fungal targets from human urine or swab samples. (seqonce.com)

However, owing to limitations in coverage of these universal primers, some microorganisms remain unidentified. (biomedcentral.com)

In this case, imperfect probe-target binding can easily occur, resulting in false-positive outcomes such as mistaking a strain that is commensal for one that is pathogenic. (wikipedia.org)
Nucleic acid tests use a "probe" which is a long strand with a short strand stuck to it. (wikipedia.org)
The disease strand sticks tightly to the exposed part of the long primer strand (called the "toehold"), and then little by little, displaces the short "protector" strand from the probe. (wikipedia.org)
When the correct target(X) reacts with the toehold exchange probe(PC), P is released and hybridized product XC is formed. (wikipedia.org)
On the other hand, if the toehold exchange probe(PC) reacts with spurious target(S), the reaction forwards, but the standard free energy increases to be less thermodynamically favorable. (wikipedia.org)
The GEN-PROBE APTIMA trichomonas vaginalis assay combines the technologies of target capture, Transcription-Mediated Amplification (TMA), and Dual Kinetic Assay (DKA). (cdc.gov)
A single-stranded chemiluminescent DNA probe, which is complementary to a region of the target amplicon, is labeled with different acridinium ester molecule. (cdc.gov)
Therefore, primer dimers are an issue in reactions using both probe-based and SYBR Green I dye-based detection. (sigmaaldrich.com)
These customizable parameters let you select primer, probe, and amplicon criteria to target regions. (idtdna.com)
The primer and probe set can be used on blood samples for both a one-step and two-step RT-PCR. (nih.gov)
Reactions were performed with 0.2 μM of each primer, 0.1 μM of probe, and iTaq Supermix (Bio-Rad, Rheinach, Switzerland). (cdc.gov)
The panel is provided in plate format with each probe/primer target in one well. (seqonce.com)

The results showed that those 2 isolates were multi-drug resistant and the DNA sequencing analysis showed that the alignment of nucleic acid of DNA in isolates of mycobacteria other than M. tuberculosis was different from that of M. tuberculosis complex. (who.int)

Experiments were designed to amplify a DNA target (BRCAb) and a RNA target (HMBS2) by LAMP or RT-LAMP, respectively. (neb.com)
During the LAMP reaction, several primer sets are used to amplify multiple distinct regions of target nucleic acid. (labx.com)

Such NATs are called nucleic acid amplification tests (NAATs). (wikipedia.org)

Although capable of sensitive, rapid detection and quantification of a particular target, PCR is limited when multiple loci are targeted by primers," the researchers wrote in Cell Reports Methods . (darkdaily.com)
The approach relies on a blend of structural, biochemical, and biophysical methods to define the architecture, function, evolution, and regulation of protein/nucleic acid complexes. (hopkinsmedicine.org)
Recent technological advances have seen the introduction of methods that detect mycobacteria by amplification of mycobacterial nucleic acids (DNA or RNA) followed by detection of the amplification product. (vin.com)
Therefore, universal primer-independent sequencing library construction methods are needed. (biomedcentral.com)
Modified meta-transcriptomic methods that were used to enrich SSU rRNA without universal primers [ 26 ] provided an opportunity to discover active microbial dark matter from the "rare biosphere" not covered by universal primers. (biomedcentral.com)
The present invention relates to methods and systems for nucleic acid sequencing. (justia.com)
In particular, the present invention relates to methods and systems for reducing the number of false-positives in nucleic acid sequencing. (justia.com)
Accordingly, the technical problem underlying the present invention is the provision of means and methods to provide a reliable way of nucleic acid sequencing, in particular the identification of variations in the genome. (justia.com)

In addition, the culture was immunofluorescently labeled with Cy2 conjugated to antibodies that target peroxisomal membrane protein 70 ( PMP 70 ), an abundant and integral membrane component of peroxisomes. (fsu.edu)
Analysis with the both the 120-kDa protein primers and the VLPT primers showed that genetic variance exists. (google.com)
Dr. Berger's laboratory is also exploring whether the protein machinery responsible for initiating DNA replication can be exploited as a novel anti-cancer target. (hopkinsmedicine.org)
The amino acid is added to a growing protein during the process of translation. (wikibooks.org)

Bromodomains are proteins that contain modules of ~110 amino acids that recognize and bind acetylated lysine residues in histones and other proteins. (frontiersin.org)
The project aims to study how posttranslational modifications with ubiquitin and ubiquitin-like proteins regulate the DNA damage response, and how dysregulation of these pathways in cancer cells could be exploited towards the development of novel anti-cancer drug targets. (findaphd.com)
Dr. Berger's team is also studying the nucleic acid-dependent motors, with a particular focus on a variety of DNA- and RNA-dependent motor proteins. (hopkinsmedicine.org)
They are working to determine how such proteins interact with nucleic acids and partner proteins, and how they use ATP to drive the architectural changes required for catalysis and physical movement. (hopkinsmedicine.org)

Mouse Embryo Fibroblast Cells with Texas Red-X, Alexa Fluor 488, and DAPI - In this section, a Swiss mouse embryo fibroblast cell is presented that was resident in a culture labeled with the fluorophore Texas Red-X conjugated to wheat germ agglutinin, a fluorescent lectin that selectively binds to sialic acid residues. (fsu.edu)

Amplification products were detected in real-time with a molecular beacon (FAM labelled/methyl-red quenched) that recognised an internal region of the target amplicon. (int-res.com)

Their function is regulated by the activation of a number of activating and inhibitory receptors that bind to specific ligands expressed on the surface of target cells. (frontiersin.org)

Irrespective of the LAB biodiversity, a large majority of the sugar and amino acid metabolites were detected in all sourdough samples. (who.int)

However, the mutants which form the genetic basis for a variety of human diseases are usually slightly different from the normal nucleic acids. (wikipedia.org)
Nucleic acids convey genetic information. (spilianakislab.gr)

Wooden shafts or calcium alginate swabs may contain substances that inhibit some viruses and nucleic acid detection reactions. (medscape.com)
Next generation sequencing (NGS) of nucleic acid extracts from plants infected with unknown viruses is expected to yield information about the genomes of previously uncharacterized viruses without any background information on what viruses might be infecting the plant. (usda.gov)

Similarly, when multiple targets are to be detected simultaneously in multiplex reactions, assay conditions must be optimized to detect all targets equally. (sigmaaldrich.com)

Whenever primer dimer products are produced and amplified, they divert reaction components away from synthesis of the desired product, thereby reducing assay efficiency and sensitivity. (sigmaaldrich.com)
However, in clinical trials, these tests have shown approximately a 99% sensitivity at detecting acid fast positive, culture positive samples, but only a 60-80% sensitivity at detecting acid fast negative, culture positive samples. (vin.com)
Sensitivity of 1 copy per µl for most targets, depending on chosen extraction method. (seqonce.com)

Optimization is required to ensure that the assay is as sensitive as is required and that it is specific to the target of interest. (sigmaaldrich.com)
For SNP detection, the LNA residue is placed at the 3' end of the allele specific PCR primer. (usda.gov)
We showed that using specific primers designed based on universal primer evaluation from meta-transcriptomic datasets, identification of novel taxonomic groups from a specific environment is possible. (biomedcentral.com)
Specific applications include de novo sequencing and re-sequencing of genomes, metagenomics, RNA analysis, and targeted sequencing of DNA regions of interest. (pressebox.com)

DNA is a long chain of linear polymers containing deoxyribose sugars and their covalently bonded bases known as nucleic acids. (wikibooks.org)
Dr Richard Owczarzy (Ohv-char'-zee), a Principal Scientist at IDT and an extensively published expert on nucleic acid thermodynamics, confirms that many researchers are still under the illusion that they can use a simple formula to calculate T m -multiplying the number of GC and AT bases by a given constant. (idtdna.com)

The microparticles, including the captured target molecules bound to them, are pulled to the side of the reaction vessel using magnets and the supernatant is aspirated. (cdc.gov)

The McMaster scientists were motivated by the "challenges of low signal, high background, and uncertain targets that plague many metagenomic sequencing efforts," they noted in their paper. (darkdaily.com)
Our unique QIAseq Multimodal sequencing panels provide the only one-day consolidated DNA and RNA library prep workflow that requires just 10 ng total nucleic acid input. (qiagen.com)

These were followed by quantification of single or multiplexed targets (10%), then other areas (7%) and basic research (5%) (Figure 2). (ddw-online.com)

An early-morning sputum sam- encoding the B-subunit of RNA of droplet particles aerosolized from ple was collected from 170 patients polymerase ( rpoB DNA, 342-360 persons infected with Mycobacterium into wide-mouthed plastic contain- base pairs) was the target region for tuberculosis or by consumption of milk ers. (who.int)

Two disease pathogens the researchers specifically targeted in their study are responsible for sepsis and SARS-CoV-2 , the coronavirus causing COVID-19 . (darkdaily.com)
By shifting the task of primer design to IDT's online tool, researchers can now focus their efforts on making discoveries. (pressebox.com)
Integrated DNA Technologies (IDT) is the largest supplier of custom nucleic acids in the United States, serving academic, government, and commercial researchers in biotechnology, clinical diagnostics, and pharmaceutical development. (pressebox.com)

Locked nucleic acid (LNA) single nucleotide polymorphism (SNP) genotype analysis and validation using real-time PCR. (cdc.gov)
In addition, extensive metabolite target analysis of more than 100 different compounds was performed. (who.int)

Amplification of small subunit (SSU) rRNA genes with universal primers is a common method used to assess microbial populations in various environmental samples. (biomedcentral.com)
These nucleic acid polymers encode for the all of the materials an organism needs to live in the form of genes. (wikibooks.org)

The loops formed by the loop primers facilitate subsequent rounds of exponential amplification. (labx.com)

A 25 μl reaction containing 10 ng HeLa DNA or 5 ng Jurkat total RNA, 1X Master Mix, 1X LAMP primers and fluorescent dye was incubated on a Bio-Rad CFX98 instrument at 65°C for 75 minutes. (neb.com)
The capture oligomer:target complex is then captured out of solution by decreasing the temperature of the reaction to room temperature. (cdc.gov)
The engineered WarmStart Luna Reverse Transcriptase also possesses higher thermostability than many other RTs, allowing an optimal reaction temperature of 55°C. For difficult targets/templates, higher RT step temperatures of up to 60°C can be used without compromising Luna performance. (neb.com)

A number of powerful applications are made possible by the system's highly accurate long reads and simplified by this new primer design tool. (pressebox.com)

The partnership offers Genome Sequencer FLX System users a convenient solution for primer creation, streamlining workflow and relieving bioinformatics burdens. (pressebox.com)

Acid fast smears have a lower limit of detection of about 1000 organisms/ml, whereas culture can detect approximately 100 organisms/ml. (vin.com)

Need assistance designing LAMP primers? (neb.com)
Use the NEB LAMP Primer Design Tool . (neb.com)
A RNA target (HMBS2) was amplified from Jurkat total RNA using the WarmStart LAMP Kit and OptiGene Master Mix (ISO-001). (neb.com)

DNA is a nucleic acid which is capable of duplicating itself via the enzyme known as DNA polymerase. (wikibooks.org)

Genomic comparison revealed high conservation of key metabolic features in sublineage II Nitrospira , including respiratory complexes I-V and the machineries for nitrite oxidation and carbon fixation via the reductive tricarboxylic acid cycle. (osti.gov)

Based on the detection of cell culture-derived nodavirus, and a synthetic RNA target, the real-time NASBA procedure was approximately 100-fold more sensitive than single-tube RT-PCR. (int-res.com)
Total RNA was isolated from each of 30 single mouse sclera (n=30, 6 sclera each from 1-, 2-, 3-, 6-, and 8-week-old mice) and reverse-transcribed into cDNA using a T7-N 6 primer. (molvis.org)
The Lioness UT Microbiota Panel is designed for qualitative single-plex in-vitro assessment of common urinary tract microbiota using nucleic acid amplification. (seqonce.com)

The water samples were filtered through a cellulose membrane to collect Giardia and Cryptosporidium, and then nucleic acids were extracted. (bvsalud.org)

Since it is usual to run the assay on a selected instrument and under standard buffer conditions, most optimization procedures are focussed on modification of primer binding kinetics using primer concentration or annealing temperature (T a )/melting temperature (T m ). (sigmaaldrich.com)

Although PCR or naive DNA capture can be used when there are a small number of organisms of interest, design challenges become untenable for large numbers of targets. (darkdaily.com)
Validation of primer design is particularly important when adopting primers from a previous publication or using a commercially supplied assay. (sigmaaldrich.com)
To determine the potential for primer-dimer formation, use primer design software to analyze duplex formation. (sigmaaldrich.com)
The PrimerQuest™ Tool offers you the option to customize almost 50 different parameters that will lead to the most efficient primer design for your experiment. (idtdna.com)
We are pleased to leverage IDT's significant experience in primer design and construction to offer our customers a one-stop solution for all their targeted resequencing projects,' explained Chris McLeod, CEO of 454 Life Sciences, a Roche company. (pressebox.com)

When the molecular beacon is not annealed to a complementary target the hairpin structure places the fluorophore and the quencher in close proximity preventing fluorescence. (usda.gov)
DNA secondary structure, such as hairpin formation, can affect primer performance. (pressebox.com)

Work from their group is revealing important information about the mechanisms of origin processing, primer synthesis, and macromolecular assembly that occur during replisome construction. (hopkinsmedicine.org)
In most cases of natural DNA replication, the primer for DNA synthesis and replication is a short strand of RNA (which can be made de novo). (khanacademy.org)

The polymerase starts replication at the 3'-end of the primer, and copies the opposite strand. (khanacademy.org)

In the end, the short protector strand is not bound to anything, and the unbound short primer is detectable. (wikipedia.org)
A strand-displacing polymerase and an additional pair of "loop primers" are used as well. (labx.com)
Direct link to Kevin D. Fettel's post "A primer is a strand of s. (khanacademy.org)
Comment on Kevin D. Fettel's post "A primer is a strand of s. (khanacademy.org)
He mentions it briefly in the video, in the liquid there are lots of DNA primers around, so when it cools down it is more likely the primer will attach to the DNA strand (and thereby blocking another DNA strand from attaching, rather than another DNA strand merging. (khanacademy.org)

In this study we examine the use of molecular beacons and locked nucleic acids (LNAs) to identify genotypes carrying alleles at rhg1 and rhg4 for resistance or susceptibility. (usda.gov)

Anatomy4

Organisms11

Diseases2

Chemicals and Drugs105

Analytical, Diagnostic and Therapeutic Techniques and Equipment47

Phenomena and Processes67

Disciplines and Occupations6

Anthropology, Education, Sociology and Social Phenomena2

Technology, Industry, Agriculture6

Information Science10

Health Care7

Probes14

Sequences15

Specificity9

Hybridization7

Reagents1

Sequence13

Strands4

Polymerase chain re1

Gene8

Isothermal amplification1

QPCR5

Concentrations4

Viral3

Microorganisms1

Probe12

Isolates1

Amplify2

NAATs1

Methods8

Protein4

Proteins4

Residues1

Region of the target amplicon1

Bind1

Amino acid1

Genetic2

Viruses2

Reactions1

Sensitivity3

Specific4

Bases2

Molecules1

Sequencing2

Quantification1

Particles1

Researchers3

Analysis2

Genes2

Exponential1

Reaction3

Reads1

Workflow1

Detect1

LAMP3

Enzyme1

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Single3

Membrane1

Temperature1

Design5

Hairpin2

Synthesis2

Replication1

Strand5

Molecular1