• Liquid-liquid extraction was followed by high-performance liquid chromatography-electrospray ionization-tandem mass spectrometry. (ojp.gov)
  • We describe here a new reversed-phase high-performance liquid chromatography with mass spectrometry detection method for quantifying intact cytokinin nucleotides in human K-562 leukemia cells. (cas.cz)
  • Title : Two-dimensional high performance liquid chromatography separation and tandem mass spectrometry detection of atrazine and its metabolic and hydrolysis products in urine☆ Personal Author(s) : Kuklenyik, Zsuzsanna;Panuwet, Parinya;Jayatilaka, Nayana K.;Pirkle, James L.;Calafat, Antonia M. (cdc.gov)
  • Separation was performed on a pentaflurophenyl column (150*2mm,3μm) using a ‎mobile phase consisting 70% acetonitrile and 30% ammonium acetate (10mM, pH3.5) with a ‎high performance liquid chromatography system coupled with tandem mass spectrometry‏. (analchemres.org)
  • High-performance liquid chromatography coupled to tandem mass spectrometry was used to develop and validate a rapid method to qualitatively and quantitatively analyse 18 common adulterants in herbal medicine and food samples. (elsevierpure.com)
  • Bern, M. W. Preview: a program for surveying shotgun proteomics tandem mass spectrometry data. (parc.com)
  • De novo peptide sequencing algorithms have been widely used in proteomics to analyse tandem mass spectra (MS/MS) and assign them to peptides, but quality-control methods to evaluate the confidence of de novo peptide sequencing are lagging behind. (arxiv.org)
  • Tandem mass spectrometry(MS/MS) of peptides is a central technology for Proteomics, enabling the identification of thousands of peptides from a complex mixture. (princeton.edu)
  • Water chromatography in conjunction with tandem mass spectrometry has revolutionized the proteomics evaluation of complexes tissue and cells. (cancer-ecosystem.com)
  • Using proteomics data produced on various kinds of mass spectrometers we optimized the De-Noise algorithm predicated on the quality and mass precision from the mass spectrometer used in the LC/MS/MS test. (cancer-ecosystem.com)
  • A team of scientists the Sports Medicine Research and Testing Laboratory at the University of Utah have developed a test that makes use of liquid chromatography-tandem mass spectrometry. (science20.com)
  • To investigate the pharmacokinetics of arotinoid acid, a sensitive and selective liquid chromatographic-tandem mass spectrometric method (LC-MS/MS) for the determination of arotinoid acid in human plasma was developed and validated. (unboundmedicine.com)
  • TY - JOUR T1 - Determination of arotinoid acid in human plasma by liquid chromatography-tandem mass spectrometry. (unboundmedicine.com)
  • Here a liquid chromatography tandem mass spectrometry (LC-MS/MS) method for the simultaneous analyses of multiple steroid hormones in cetacean blubber is validated and applied to 10 stranded humpback whales (Megaptera novaeangliae). (nist.gov)
  • Therefore, in the present study, we performed untargeted lipid profiling of abalone tissues at different developmental stages as well as the hemolymph of mature female and male abalones using ultrahigh-performance liquid chromatography-tandem mass spectrometry. (hindawi.com)
  • A fast and sensitive ultra performance liquid chromatography-tandem mass spectrometry (UPLC-MS/MS) method was developed for the determination and pharmacokinetic study of nimodipine in human plasma. (unboundmedicine.com)
  • The purposes of this study were to develop a sensitive, specific liquid chromatography-tandem mass spectrometry (LC-MS/MS) method for detection of 25-hydroxyvitamin D(2) and D(3) and establish reference intervals for these analytes. (nih.gov)
  • The determination of salbutamol and its glucuronide in human urine following the inhalative and oral administration of therapeutic doses of salbutamol preparations was performed by means of direct urine injection utilizing liquid chromatography-tandem mass spectrometry (LC-MS/MS) and employing d3-salbutamol and d3-salbutamol glucuronide as internal standards. (uni-koeln.de)
  • Determination of mycotoxins in tobacco and smokeless tobacco products by liquid chromatography tandem mass spectrometry. (coresta.org)
  • To identify the MAO-B substrate in PVT, we generated Maob knockout (KO) mice and measured five candidate substrates (i.e., noradrenaline, dopamine, 3-methoxytyramine, serotonin, and phenethylamine [PEA]) by liquid chromatography tandem mass spectrometry. (sciencegate.app)
  • Quantification of Folic acid (FA) in fortified wheat flour was carried out using a triple quadrupole liquid chromatography tandem mass spectrometry method. (eurekaselect.com)
  • Liquid chromatography-tandem mass spectrometry (LC-MS/MS) is increasingly utilized in clinical laboratories because it has advantages in terms of specificity and sensitivity over other analytical technologies. (annlabmed.org)
  • Simultaneous determination of nine estrogens in bullfrogs using filtered solid phase extraction and ultra-performance liquid chromatography-tandem mass spectrometry]. (bvsalud.org)
  • Therefore, a rapid and accurate method based on PRiME HLB solid phase extraction (SPE) cartridge purification and ultra-performance liquid chromatography - tandem mass spectrometry (UPLC-MS/MS) was developed to determine nine estrogen residues in bullfrogs . (bvsalud.org)
  • Cotinine was quantified by liquid chromatography tandem mass spectrometry. (lu.se)
  • An interchain cross-link between two beta subunits was found in two tandem mass spectra. (nih.gov)
  • Here we describe a program called Preview that analyzes a set of mass spectra for mass errors, digestion specificity, and known and unknown modifications, thereby facilitating parameter selection. (parc.com)
  • Currently, the qualitative seized-drug analysis is mainly performed by gas chromatography-electron ionization-mass spectrometry (GC-EI-MS) in which most of these emerging designer drug derivatives are extensively fragmented, not presenting a molecular ion in their mass spectra. (ojp.gov)
  • In addition, CI mass spectra and MRM mass spectra of these designer drug derivatives can be used as a potential supplemental database along with EI mass spectral database. (ojp.gov)
  • and the second step finds the best cross-linked amino acids in these two peptide sequences that are optimally correlated to a given tandem mass spectrum. (nih.gov)
  • Database search programs for peptide identification by tandem mass spectrometry ask their users to set various parameters, including precursor and fragment mass tolerances, digestion specificity, and allowed types of modifications. (parc.com)
  • The molecules of a given sample are ionized and the first spectrometer (designated MS1) separates these ions by their mass-to-charge ratio (often given as m/z or m/Q). Ions of a particular m/z-ratio coming from MS1 are selected and then made to split into smaller fragment ions, e.g. by collision-induced dissociation, ion-molecule reaction, or photodissociation. (wikipedia.org)
  • These fragments are then introduced into the second mass spectrometer (MS2), which in turn separates the fragments by their m/z-ratio and detects them. (wikipedia.org)
  • Typical tandem mass spectrometry instrumentation setups include triple quadrupole mass spectrometers (QqQ), multi-sector mass spectrometer, quadrupole-time of flight (Q-TOF), Fourier transform ion cyclotron resonance mass spectrometers, and hybrid mass spectrometers. (wikipedia.org)
  • Q-TOF mass spectrometer combines TOF and quadrupole instruments, which cause high mass accuracy for product ions, accurate quantitation capability, and fragmentation experiment applicability. (wikipedia.org)
  • Hybrid mass spectrometer consists of more than two mass analyzers. (wikipedia.org)
  • Multiple stages of mass analysis separation can be accomplished with individual mass spectrometer elements separated in space or using a single mass spectrometer with the MS steps separated in time. (wikipedia.org)
  • and BEBE - four-sector (reverse geometry) mass spectrometer. (wikipedia.org)
  • The detection was performed on a triple quadrupole tandem mass spectrometer by multiple reaction monitoring (MRM) mode via electrospray ionization (ESI) source. (unboundmedicine.com)
  • Analytes were quantified in negative selected ion monitoring mode using a single quadrupole mass spectrometer. (cas.cz)
  • The analyte ions of interest were guided into an ion trap mass spectrometer for tandem mass analysis. (elsevierpure.com)
  • Gcn4 complex and its own MudPIT evaluation using an LCQ quadrupole ion snare mass spectrometer (Thermofisher) have already been previously referred to 19. (cancer-ecosystem.com)
  • Subsequently, phosphatidylcholine, lysophosphatidyl¬choline, sphingo-myelin, ceramide, phosphatidylethanolamine, phosphatidylethanolamine-based plasmalogen, cholesterol and cholesteryl ester content of the separated lipoproteins was quantified by electrospray ionization tandem mass spectrometry (ESI-MS/MS). Results: Analysis of FPLC fractions with polyacrylamide gel electrophoresis demonstrated that albumin partially co-elutes with HDL fractions. (uni-regensburg.de)
  • Product-ion traces at m/z 120.141, 176.055, and 295.074 were used for quantitation of FA, and traces at m/z 442.236 were used as parent mass quantified using positive heated electrospray ionization (HESI).The validated method showed linearity ranging from 1.25 to 640 ng/ml of FA and a correlation coefficient of 0.996. (eurekaselect.com)
  • A novel sensitive method utilizing nano extractive electrospray ionization mass spectrometry (nanoEESI-MS) has been developed for the rapid analysis of aerosol drug samples with quantitative information. (elsevierpure.com)
  • In this study, the qualitative identification of 34 designer drugs, mainly synthetic cannabinoids and synthetic cathinones, was performed by gas chromatography-triple quadrupole-tandem mass spectrometry with two different ionization techniques, including electron ionization (EI) and chemical ionization (CI), focusing only on qualitative seized-drug analysis, not from the toxicological point of view. (ojp.gov)
  • 140 mouse brain proteins identified by Ca2+-calmodulin affinity chromatography and tandem mass spectrometry. (lu.se)
  • This experiment is commonly performed to identify transitions used for quantification by tandem MS. In a neutral loss scan, the first mass analyzer scans all the masses. (wikipedia.org)
  • Extraction and quantification of total folate using a microbiological assay (MA) with Lactobacillus casei as the assay organism and Enzyme Linked Immuno Sorbent Assay (ELISA) by competitive binding rapid ELISA kit was also performed. (eurekaselect.com)
  • In tandem mass spectrometry in space, the separation elements are physically separated and distinct, although there is a physical connection between the elements to maintain high vacuum. (wikipedia.org)
  • By doing tandem mass spectrometry in time, the separation is accomplished with ions trapped in the same place, with multiple separation steps taking place over time. (wikipedia.org)
  • A common use of tandem MS is the analysis of biomolecules, such as proteins and peptides. (wikipedia.org)
  • A set of tools for detecting differentially abundant peptides and proteins in shotgun mass spectrometry-based proteomic experiments with tandem mass tag (TMT) labeling. (bioconductor.org)
  • These problems include (i) estimating the levels of the thousands of proteins in a tissue sample, (ii) predicting the intensity of the peaks in a mass spectrum, and (iii) explaining why different peptides from the same protein have different peak intensities. (princeton.edu)
  • This paper reports a simple and reliable mass spectrometry hyphenated chromatographic method for ochratoxin A determination in hard cheese. (unipr.it)
  • The fragmentation step makes it possible to identify and separate ions that have very similar m/z-ratios in regular mass spectrometers. (wikipedia.org)
  • A research team has developed a new approach for selecting precursor ions in tandem mass spectrometry (MS/MS) experiments called DiffN, which increases the likelihood of biomarker discovery and provides a promising tool for lipidomic analyses. (chromatographyonline.com)
  • Researchers at the University of North Carolina at Chapel Hill have developed a novel approach for selecting precursor ions in tandem mass spectrometry (MS/MS) experiments. (chromatographyonline.com)
  • This is a method of mass spectrometry that ion fragmentation (m/z) ratio determined through a time of flight measurement. (wikipedia.org)
  • Cross-linking technology combined with tandem mass spectrometry (MS-MS) is a powerful method that provides a rapid solution to the discovery of protein-protein interactions and protein structures. (nih.gov)
  • The development of the DiffN method represents an important advancement in tandem mass spectrometry, enhancing the ability to detect low-abundance species with significant biological implications. (chromatographyonline.com)
  • Three mass transitions for each analyte were evaluated to enhance the selectivity and accuracy of the method. (coresta.org)
  • A method for the simultaneous measurement of buprenorphine and its N- dealkylated metabolite, norbuprenorphine, in human plasma was developed with negative chemical ionization tandem mass spectrometry. (johnshopkins.edu)
  • Tandem mass spectrometry (MS/MS) is the main screening method for inborn errors of metabolism (IEMs). (cdc.gov)
  • Triple quadrupole mass spectrometers use the first and third quadrupoles as mass filters. (wikipedia.org)
  • Here, we report specific and sensitive determinations for CLN1, CLN2, and CLN10 on dried blood spots by tandem mass spectrometry using multiple reaction monitoring mass spectrometry (MRM-MS). Identical substrates suitable for (i) fluorimetric determination of single enzymes and (ii) for MRM-MS determination of multiple enzymes were synthesized by chemical coupling of alkyl-umbelliferone building blocks with the corresponding peptidyl-substrate groups recognized by the target enzyme. (uni-konstanz.de)
  • Each instrumental configuration utilizes a unique mode of mass identification. (wikipedia.org)
  • Moreover, Preview optionally recalibrates mass over charge measurements, leading to further improvement in identification results. (parc.com)
  • The implementation of CI source facilitates the determination of molecular mass and the identification of seized designer drugs. (ojp.gov)
  • Tandem mass spectrometry was used to confirm the identification of important compounds in each aerosol drug sample. (elsevierpure.com)
  • Tandem mass spectrometry, also known as MS/MS or MS2, is a technique in instrumental analysis where two or more mass analyzers are coupled together using an additional reaction step to increase their abilities to analyse chemical samples. (wikipedia.org)
  • Reaction gas methods can use either on-mass or mass-shift measurement. (spectroscopyonline.com)
  • Tandem MS-MS analysis was performed in selective reaction monitoring mode (SRM). (eurekaselect.com)
  • Determination of the toluene diisocyanate binding sites on human serum albumin by tandem mass spectrometry. (cdc.gov)
  • To better understand the chemical species produced when diisocyanates react with protein, tandem mass spectrometry was employed to unambiguously identify the binding sites of the industrially important isomers, 2,4- and 2,6-toluene diisocyanate on human serum albumin at varying diisocyanate:protein ratios. (cdc.gov)
  • Tandem in time MS instruments do not use the modes described next, but typically collect all of the information from a precursor ion scan and a parent ion scan of the entire spectrum. (wikipedia.org)
  • For a precursor ion scan, the product ion is selected in the second mass analyzer, and the precursor masses are scanned in the first mass analyzer. (wikipedia.org)
  • In a product ion scan, a precursor ion is selected in the first stage, allowed to fragment and then all resultant masses are scanned in the second mass analyzer and detected in the detector that is positioned after the second mass analyzer. (wikipedia.org)
  • The molecular anion for buprenorphine was used as a precursor ion, whereas the [M-197] - was used as a precursor ion for norbuprenorphine in tandem mass spectrometry. (johnshopkins.edu)
  • At the time of publication of this document, approximately 98% of all babies born in the United States are provided with expanded newborn screening for metabolic diseases by tandem mass spectrometry. (aacc.org)
  • Purpose of review: To summarize recent developments in the field of newborn screening related to the use of tandem mass spectrometry as an analytic platform. (elsevierpure.com)
  • Summary: Although it is done inconsistently, newborn screening in the United States is undergoing a rapid expansion driven by the introduction of tandem mass spectrometry in at least 34 state programs. (elsevierpure.com)
  • Specific peptidyl-(4-alkyl-umbelliferone) substrates were also synthesized for mass spectrometric determinations of different cathepsins (cathepsins-D, -F, and -B), to provide a differentiation of proteolytic specificities. (uni-konstanz.de)
  • The increased numbers of different metabolic diseases that are identifiable by tandem mass spectrometry also increases the complexity of testing required for confirmation of diagnosis. (aacc.org)
  • In addition, tandem mass spectrometry is being utilized for multiple additional metabolic conditions, which vary by state. (aacc.org)
  • This application is difficult for conventional quadrupole inductively coupled plasma-mass spectrometry (ICP-MS) because of the presence of matrix-based spectral interferences on the isotopes of Ti. (spectroscopyonline.com)
  • With the increasing acquisition rate of tandem mass spectrometers, there is an increasing potential to solve important biological problems by applying data-mining and machine-learning techniques to MS/MS data. (princeton.edu)
  • There are a number of different tandem MS/MS experimental setups and each mode has its own applications and provides different information. (wikipedia.org)
  • Further research and implementation of the DiffN approach in various analytical settings will shed light on its broader applications and impact in the field of mass spectrometry. (chromatographyonline.com)
  • Our data demonstrate that nanoEESI tandem mass spectrometry is a fast and sensitive technique for the analysis of aerosol drug preparations, showing promising applications in pharmacology studies and in situ analysis of aerosol drugs on the market. (elsevierpure.com)
  • Uhl M. "Tandem mass spectrometry: a helpful tool in hair analysis for the forensic expert" Forensic Sci Int . 2000;107(1-3):169-79. (erowid.org)
  • The instrumentation used for hair analysis is a powerful analytical tool: a Varian 3400 gas chromatograph linked to a Finnigan Tandem-MS (TSQ 700). (erowid.org)
  • Tandem differential mobility analysis-mass spectrometry reveals partial gas-phase collapse of the GroEL complex. (ox.ac.uk)
  • Aim Tandem mass spectrometry (MS/MS) is a recommended investigation for sudden unexpected death in infancy (SUDI), but there are limited data regarding yield and potential influencing factors. (bmj.com)
  • Tandem MS in space uses the coupling of two instrument components which measure the same mass spectrum range but with a controlled fractionation between them in space, while tandem MS in time involves the use of an ion trap. (wikipedia.org)
  • The samples were derivatized with heptafluorobutyric anhydride and measured with negative chemical ionization tandem mass spectrometry. (johnshopkins.edu)
  • When using multiple quadrupoles, they can act as both mass analyzers and collision chambers. (wikipedia.org)
  • The molecular mass of SARS-CoV-2 M pro as determined by mass spectroscopy is 33797.0 Da, consistent with its theoretical molecular mass of 33796.8 Da. (nature.com)
  • A new mass spectrometry test can help sports anti-drug doping officials to detect whether an athlete has used drugs that boost naturally occurring steroid levels. (science20.com)
  • abstract = "Homopolymers and copolymers of alkylisocyanates having n-hexyl, 2,6-dimethylheptyl, 3,7-dimethyloctyl, and (2,2-dimethyl-1,3-dioxolan-4-yl)methyl substituents underwent thermal degradation in the course of desorption electron ionization to yield trimers and monomers that were characterized in situ by tandem mass spectrometry. (nyu.edu)
  • There was significant association between blood acylcarnitine findings and postmortem interval (PMI), body mass index and liver weight. (bmj.com)