Tandem homology domain binds. Medical search. Frequent questions

It does not share significant sequence homology with other subtypes of small G-protein GEF motifs such as the Cdc25 domain and the Sec7 domain, which specifically interact with Ras and ARF family small GTPases, respectively, nor with other Rho protein interactive motifs, indicating that the Dbl family proteins are evolutionarily unique. (embl-heidelberg.de)
Although there is no extended sequence homology between these two different F-actin binding domains, our experiments show that they are functionally equivalent and provide new insights into the mechanism of microfilament severing. (silverchair.com)
PPL1 has two tandem carbohydrate recognition domain (CRD) structures in its sequence which has sequence homology with rhamnose-binding lectins (RBLs) from various fish eggs and a galactose-binding lectin (SUEL) from sea urchin eggs [24]. (cancer-ecosystem.com)
20%-21% protein sequence homology. (cdc.gov)

All of the proteins bind ATP and, consequently, all of them carry the classical Walker A (phosphate-binding loop or P-loop) and Walker B (Mg2+-binding aspartic acid) motifs. (embl.de)
There are 499956 DEXDc domains in 496764 proteins in SMART's nrdb database. (embl.de)
Taxonomic distribution of proteins containing DEXDc domain. (embl.de)
The complete taxonomic breakdown of all proteins with DEXDc domain is also avaliable . (embl.de)
Click on the protein counts, or double click on taxonomic names to display all proteins containing DEXDc domain in the selected taxonomic class. (embl.de)
Like all members of the Ras superfamily, the Rho proteins cycle between active GTP-bound and inactive GDP-bound conformational states. (embl-heidelberg.de)
Activation of Rho proteins through release of bound GDP and subsequent binding of GTP, is catalysed by guanine nucleotide exchange factors (GEFs) in the Dbl family. (embl-heidelberg.de)
The proteins encoded by members of the Dbl family share a common domain, presented in this entry, of about 200 residues (designated the Dbl homology or DH domain) that has been shown to encode a GEF activity specific for a number of Rho family members. (embl-heidelberg.de)
There are 39736 RhoGEF domains in 38458 proteins in SMART's nrdb database. (embl-heidelberg.de)
The Rho family of GTP-binding proteins has been implicated in the regulation of various cellular functions including actin cytoskeleton-dependent morphological change. (embl-heidelberg.de)
An amino-terminal portion conserved among a subset of Dbl family proteins is sufficient for the binding of Gbetagamma. (embl-heidelberg.de)
Many actin-binding proteins contain calponin homology (CH) domains, but the manner in which these domains interact with F-actin has been controversial. (egelmanlab.org)
We show that the tandem CH domains of alpha-actinin bind F-actin in an open conformation, explaining mutations that cause human diseases and suggesting that the opening of these domains may be one of the main regulatory mechanisms for proteins with tandem CH domains. (egelmanlab.org)
There are 1194 GYF domains in 1181 proteins in SMART's nrdb database. (embl-heidelberg.de)
Mutagenesis and NMR analysis demonstrated that the CD2 binding region of CD2BP2 includes a 17-aa motif (GPY[orF]xxxxM[orV]xxWxxx GYF), also found in several yeast and Caenorhabditis elegans proteins of unknown function. (embl-heidelberg.de)
Two uncharacterized tyrosine-phosphorylated proteins of 40 and 65 kDa are uniquely precipitated by a GST fusion protein containing the tandem syk-SH2 domains in collagen-stimulated platelets. (elsevierpure.com)
RNA-binding proteins of the PUF family share a conserved domain consisting of tandemly repeated 36-40 amino acid motifs (typically eight) known as Puf repeats. (biomedcentral.com)
Proteins containing tandem repeats are often dominant targets of humoral responses during infectious diseases. (biomedcentral.com)
Their importance has been even further enhanced by the homology now identified between them and the membrane attack complex/perforin (MACPF) family of proteins, which includes several components of the complement cascade as well as perforin itself. (ox.ac.uk)
In their bHLH domains, TWIST1 is 48% and 29% identical to the murine NEUROD1 and E47 proteins. (proteopedia.org)
The Crk family of adaptor proteins (Crk I, Crk II and CrkL) are Src Homology 2 (SH2) and Src Homology 3 (SH3) domain-containing proteins that form protein complexes important for transmiting signals downstream of tyrosine kinases. (ecmbio.com)
We demonstrated that the ligand binding domain of Ear2 is required for physical and functional interaction between the two proteins. (biomedcentral.com)

There are also five conserved basic residues in the G3 SCR that are atypical of the SCR superfamily, and homology modelling showed that all five were located on one surface of the SCR. (brighton.ac.uk)
The beta cluster contains 16 genes and 3 pseudogenes, each encoding 6 extracellular cadherin domains and a cytoplasmic tail that deviates from others in the cadherin superfamily. (nih.gov)

Both contain tandem repeats of approximately 125 amino acids, the first of which is shown to contain the actin-binding site. (silverchair.com)
Both pufferfish genomes encode five tenascin genes: two tenascin-C paralogs, a tenascin-R with domain organization identical to mammalian and avian tenascin-R, a small tenascin-X with previously undescribed GK repeats, and a tenascin-W. Four tenascin genes corresponding to tenascin-C, tenascin-R, tenascin-X and tenascin-W were also identified in the X. tropicalis genome. (biomedcentral.com)
In birds and mammals, tenascin-R genes encode 4.5 EGF-like repeats and 9 FN type III domains. (biomedcentral.com)
This tenascin has 18.5 EGF-like repeats, and the tenascin-X genes of mouse and human encode 29 and 32 FN type III domains, respectively. (biomedcentral.com)
α-Actinin monomers contain three distinct domains: an N-terminal actin binding domain (ABD), four spectrin-like repeats (SRs), and a C-terminal EF hands (calmodulin like domain). (biomedcentral.com)
(A) BRCA2 protein schematic depicting domain organization: N-terminus, BRC repeats DNA binding domain (DBD), and C-terminal domain (CTD). (frontiersin.org)
By screening an L. infantum expression library with sera from human VL patients or infected hamsters, they identified 43 genes encoding B-cell antigens, and, remarkably, 44% of those antigens contained tandem repeats in their structure. (biomedcentral.com)

Opening of tandem calponin homology domains regulates their affinity for F-actin. (egelmanlab.org)
Hence, a proline-binding module distinct from SH3 and WW domains regulates protein-protein interactions. (embl-heidelberg.de)
Protein Syndesmos is a novel RNA-binding protein that regulates primary cilia formation. (nih.gov)
It was determined that Ear2 negatively regulates renin expression by competing with retinoic acid receptor/ retinoid X receptor (RAR/RXR) for binding to the retinoic acid response elements (RARE) on the renin enhancer [ 13 ]. (biomedcentral.com)

There is significant homology of the sequences, approximately 250 residues, from the N-terminus. (wikipedia.org)
The CRD and SCR domains in 13 G3 sequences were each subjected to structural analysis. (brighton.ac.uk)
The DBL1 cys2 type was observed in all indicated sequences gene domains with a larger sample size are required to confirm with statistical significance observed associations with severe malaria in Indonesian samples. (research-in-field.com)
12/15 shared amino acids) and the protein sequences have 70%-82% homology. (cdc.gov)
Amino acid position is in a conserved region compared to other Aspergilli (26/31 shared amino acids) and the protein sequences have 84% homology. (cdc.gov)
The GYF domain is a novel structural fold that is involved in lymphoid signaling through proline-rich sequences. (embl-heidelberg.de)
A phylogenetic tree based on the predicted amino acid sequences of the fibrinogen-related domains demonstrates that tenascin-C and tenascin-R are the most closely related vertebrate tenascins, with the most conserved repeat and domain organization. (biomedcentral.com)
The fastest and most common way to annotate the repetitive fraction of a genome assembly is to create repeat libraries derived from a set of previously identified representative repetitive sequences and screen the target genome using homology-based searches. (biomedcentral.com)
We have established homology models of the human TWIST1 homodimer and heterodimers (formed with the E12 partner) bound to their target DNA sequences. (proteopedia.org)
Although the C-terminal sequences adjacent to the bHLH domain, constitute a protein interaction site essential for TWIST function [4] [5] , we reasoned that a structural model restricted to the bHLH domains should provide additional insights into the TWIST-DNA interface and highlight functional differences between TWIST1 complexes. (proteopedia.org)
The established homology models (including DNA sequences) were visualized with the VMD 1.9.1 software. (proteopedia.org)

It includes tandem SNT-207858 N-terminal Src homology 2 (SH2) domains multiple tyrosine phosphorylation sites along with a C-terminal tyrosine kinase domains. (careersfromscience.org)
Ser-379 phosphorylation disrupts H-bonds that link the C-terminal tail to the autoinhibitory region (AIR) and the tandem Src homology 3 (SH3) domains, allowing the AIR to undergo phosphorylation to expose the SH3 pocket for p22phox binding. (reading.ac.uk)
Autoinhibition can be largely reversed by binding of active Arf6 to Grp1 and by phosphorylation of tandem PKC sites in Cytohesin-1. (umassmed.edu)
Crk II Tyr-221 (CrkL Tyr-207) phosphorylation is a negative regulatory site, while Crk Tyr-251 phosphorylation in the SH3 domain is a positive regulatory site. (ecmbio.com)
EGF stimulation induces phosphorylation of Tyr-251, which increases binding of Crk to the SH2 domain of Abl, and promotes transactivation of Abl. (ecmbio.com)

The TIM barrel contains the active site, all catalytic residues, and a Ca2+ binding site. (wikipedia.org)
Homology modelling showed that four of these residues are located on a surface region on the CRD that is separate from the Ca2+-binding residues involved in carbohydrate interactions. (brighton.ac.uk)
It is concluded that both the CRD and SCR domains in G3 possess basic residues that are atypical of their superfamilies and might be related to function, and that the G3 CRD domain shows an evolutionary relationship to the PTR domain in G1. (brighton.ac.uk)
The ICAM-1 binding appears to need the DBL2-C2 domains including 16 conserved cysteine residues [16-19], because they include get in touch with residues for ICAM-1. (research-in-field.com)
However, the perfect binding activity appears to rely on residues in the initial area of the DBL [18]. (research-in-field.com)
Position of ICAM-1 binding DBL2-C2 domains from three isolates (A4, A4tres and JDP8) uncovered significant homology, writing 16 conserved cysteine residues and several conserved hydrophobic amino acidity residues. (research-in-field.com)
Lateral chains of residues within the T-loop and E-loop of bHLH domains are visualized in orange, while DNA is represented in dark grey. (proteopedia.org)

The SH2 domains bind phosphorylated immunoreceptor tyrosine-based activation motifs (ITAMs) and therefore couple turned on immunoreceptors to multiple downstream signaling pathways. (careersfromscience.org)
The duplicated domains are encoded on single exons and contain putative integrin-binding motifs. (biomedcentral.com)

Evidence for functional homology in the F-actin binding domains of gelsolin and alpha-actinin: implications for the requirements of severing and capping. (silverchair.com)
The F-actin binding domains of gelsolin and alpha-actinin compete for the same site on actin filaments with similar binding affinities. (silverchair.com)
We have replaced the F-actin binding domain in the NH2-terminal half of gelsolin by that of alpha-actinin. (silverchair.com)
The hybrid binds two actin monomers and caps the barbed ends of filaments in the presence or absence of calcium. (silverchair.com)
Crystal structures have shown the tandem CH domains of alpha-actinin to be in a compact, closed conformation, but the interpretations of complexes of such tandem CH domains with F-actin have been ambiguous. (egelmanlab.org)
All of the disease-causing ACTN4 mutations identified to date are located within the actin-binding domain of the encoded protein, increasing its binding affinity to F-actin and leading to abnormal actin rich cellular aggregates. (biomedcentral.com)

The series of FN type III domains are interrupted in both mouse and human tenascin-X by a proline-rich stretch of about 100 amino acids. (biomedcentral.com)
Consequently, to gain further insights into the role of the interhelical loops in TWIST1 dimer function and specificity, we next focused on additional Saerthe-Chotzen associated TWIST1 variants with aberrant insertion of 7 amino acids into the interhelical loop at position 135 or 139 (Ins-135 or Ins-139) due to the presence of a 21-bp tandem repeat in the TWIST1 gene [7] . (proteopedia.org)

The N-terminus is extracellular and glycosylated, while the Cterminus is intracellular and harbours a highly conserved 150 amino acid-long Popeye domain. (uni-wuerzburg.de)
Biochemical data have established the role of the conserved DH domain in Rho GTPase interaction and activation, and the role of the tandem PH domain in intracellular targeting and/or regulation of DH domain function. (embl-heidelberg.de)
Finally, mScarlet-I-ArcNb H11 expressed as intrabody selectively bound the N-lobe and enabled co-immunoprecipitation of full-length intracellular Arc. (kawasaki-m.ac.jp)
G3 is implicated in the intracellular processing of aggrecan and contains a C-type lectin carbohydrate recognition domain (CRD), frequent occurrences of a C-terminal short complement repeat (SCR) domain, and occasionally an N-terminal epidermal growth factor domain. (brighton.ac.uk)
It was identified in the human intracellular protein termed CD2 binding protein 2 (CD2BP2), which binds to a site containing two tandem PPPGHR segments within the cytoplasmic region of CD2. (embl-heidelberg.de)
A membrane-proximal proline-rich tandem repeat, involved in cytokine production, is recognized by the intracellular CD2 binding protein CD2BP2. (embl-heidelberg.de)
The binding partners and substrates will be evaluated under both basal and elevated intracellular calcium conditions. (uky.edu)

The cap produced by the hybrid binds with lower affinity than that of gelsolin and is not stable: It dissociates from filament ends with a half life of approximately 15 min. (silverchair.com)

The carboxy-terminal alpha-beta domain is structurally very similar to the proximal one even though it is bereft of an ATP-binding site, suggesting that it may have originally arisen through gene duplication of the first one. (embl.de)
The DH domain is composed of three structurally conserved regions separated by more variable regions. (embl-heidelberg.de)
One conserved basic residue is identical in position with that of a conserved basic residue that mediates hyaluronate binding in the structurally related proteoglycan tandem repeat (PTR) domain in G1 and in link protein. (brighton.ac.uk)
The GYF sequence is part of a structurally unique bulge-helix-bulge motif that constitutes the major binding site for the CD2 tail. (embl-heidelberg.de)

A single tenascin gene was identified in the genome of C. intestinalis that encodes a polypeptide with domain features common to all vertebrate tenascins. (biomedcentral.com)
The BRCA2 R3052W mutated protein exacerbates genome instability, is unable to rescue homology-directed repair, and fails to complement cell survival following exposure to PARP inhibitors and crosslinking drugs. (frontiersin.org)

The structural homology with the RecA recombination protein covers the five contiguous parallel beta strands and the tandem alpha helices. (embl.de)
The PH domain is invariably located immediately C-terminal to the DH domain and this invariant topography suggests a functional interdependence between these two structural modules. (embl-heidelberg.de)
Based on crystal structures of three functional domains, we generated a computational structural model of the full p47phox protein. (reading.ac.uk)
Structural basis for recognition of 53BP1 tandem Tudor domain by TIRR. (nih.gov)

The Popeye domain containing (Popdc) family is a highly evolutionary conserved gene family, which shows no homology to other genes. (uni-wuerzburg.de)
The expression of Popdc1-LacZ transgene as well as Popdc1 protein was elevated in The Popeye domain containing (Popdc) family is a highly evolutionary conserved gene family, which shows no homology to other genes. (uni-wuerzburg.de)
CAMTAs participate in gene expression regulation by binding to the cis -elements in the promoter regions of numerous target genes. (frontiersin.org)
In this study, sequence and motif variability of genes with emphasis on the DBL1 and DBL2-C2 domains from field isolates collected in two different malaria endemic areas in Indonesia are reported. (research-in-field.com)

ArcNbs E5 and H11 selectively bound the N-lobe, which harbors a peptide ligand binding pocket specific to mammals. (kawasaki-m.ac.jp)
Binding experiments and mutational analyses have demonstrated the critical importance of the GYF tripeptide in ligand binding. (embl-heidelberg.de)

Specific combinations buy 23256-50-0 of website subtypes are described as short tandem website cassettes (DC). (research-in-field.com)

This PTP contains two tandem Src homology-2 domains, which function as phospho-tyrosine binding domains and mediate the interaction of this PTP with its substrates. (antikoerper-online.de)

The DH domain of Dbl has been shown to mediate oligomerisation that is mostly homophilic in nature. (embl-heidelberg.de)

The BRCA2 R3052W mutation fails to complement chemotherapeutic sensitivity and homology-directed repair functions in BRCA2 knockout cells. (frontiersin.org)

Each Mouse monoclonal to CD10 gene potentially encodes between two to seven Duffy-binding like (DBL), and cysteine-rich interdomain areas (CIDR). (research-in-field.com)
Calpain 5 has homology to other calpains in the cysteine protease domain and also has a unique C2 domain at the C-terminus. (uky.edu)

Bullfrog sialic acid-binding lectin (cSBL) is a multifunctional protein that has carbohydrate-binding and ribonuclease activities. (bvsalud.org)
Silurus asotus egg lectin (SAL) has been reported to enhance the incorporation of propidium iodide as well as doxorubicin into Burkitt's lymphoma Raji cells through binding to globotriaosylceramide (Gb3) on the cell surface. (bvsalud.org)

Bouard C, Terreux R, Hope J, Chemelle JA, Puisieux A, Ansieau S, Payen L. Interhelical loops within the bHLH domain are determinant in maintaining TWIST1-DNA complexes. (proteopedia.org)

Immunoblotting with recombinant ArcNbs fused to a small ALFA-epitope tag demonstrated binding to recombinant Arc as well as endogenous Arc from rat cortical tissue. (kawasaki-m.ac.jp)

The DBL2-C2 domains mediates binding towards the intercellular adhesion molecule 1 (ICAM-1) in a number of isolates. (research-in-field.com)

Four additional ArcNbs bound the region containing the C-lobe and C-terminal tail. (kawasaki-m.ac.jp)

X-ray crystal structures of TIRR and a designer protein bound to 53BP1 now reveal a mechanism in which an intricate binding area centered on an essential TIRR arginine residue blocks the methylated-chromatin-binding surface of 53BP1. (nih.gov)
The crystal structure of the Grp1 Sec7-PH tandem reveals a pseudosubstrate mechanism of autoinhibition in which the linker region between domains and a C-terminal amphipathic helix physically block the docking sites for the switch regions of Arf GTPases. (umassmed.edu)

Zinc-metallophospholipases C: Clostridium perfringens alpha-toxin, Bacillus cereus PLC (BC-PLC) Sphingomyelinases: B. cereus, Staphylococcus aureus Phosphatidylinositol-hydrolyzing enzymes: B. cereus, B. thuringiensis, L. monocytogenes (PLC-A) Pseudomonad phospholipases C: Pseudomonas aeruginosa (PLC-H and PLC-N) In mammals, PLCs share a conserved core structure and differ in other domains specific for each family. (wikipedia.org)

Grp1, ARNO, and Cytohesin-1 comprise a family of phosphoinositide-dependent Arf GTPase exchange factors with a Sec7-pleckstrin homology (PH) domain tandem. (umassmed.edu)

This peptide sequence is well conserved in mouse and rat CrkL, and has less than 50% homology to human Crk II. (ecmbio.com)
This peptide sequence is well conserved in mouse and rat Crk II, and has 40% homology with the conserved site in CrkL (Tyr-207). (phosphosolutions.com)

Both Crk II and CrkL are composed of a single SH2 domain, followed by two tandem SH3 domains. (ecmbio.com)
Both Crk II and CrkL are ubiquitously expressed and their SH domains are highly homologous, however both are required for mouse development and have distinct non-overlapping phenotypes in knockout mice. (ecmbio.com)

Mechanism of 53BP1 activity regulation by RNA-binding TIRR and a designer protein. (nih.gov)

Latest evidence showed a conserved ICAM-1-binding epitope is based on a subset of group A PfEMP1 domains known as DC4 including DBL [20]. (research-in-field.com)

ATP binds to the amino proximal alpha-beta domain, where the Walker A (motif I) and Walker B (motif II) are found. (embl.de)
The N-terminal domain also contains motif III (S-A-T) which was proposed to participate in linking ATPase and helicase activities. (embl.de)
Identification of a proline-binding motif regulating CD2-triggered T lymphocyte activation. (embl-heidelberg.de)
The association of syk with Fcγ-RIIA appears to be mediated through the tandem SH2 domains in syk and the ARAM motif of Fcγ-RIIA. (elsevierpure.com)

The calmodulin-binding transcription activators (CAMTA) play critical roles in plant growth and responses to environmental stimuli. (frontiersin.org)

KIN17, which is known as a DNA and RNA binding protein, is highly expressed in numerous types of human cancers and was discovered to participate in several vital cell behaviors, including DNA replication, damage repair, regulation of cell cycle and RNA processing. (spandidos-publications.com)

Here, we report that the exchange activity of the Sec7 domain is potently autoinhibited by conserved elements proximal to the PH domain. (umassmed.edu)

Has cohesin ATPase activity, double-stranded DNA binding, topological DNA entrapment activity, role in mitotic cohesin loading and condensed nuclear chromosome, centromeric region, nuclear mitotic cohesin complex localization. (cdc.gov)
The role of the C2 domain in Ca2+ binding along with CAPN5 activation and localization will be evaluated in Aim 2. (uky.edu)

Calpain 5 (CAPN5), which lacks the penta-EF hand domain of classical calpains, is the 2nd most highly expressed calpain in the CNS. (uky.edu)

Trio and its homologue UNC-73 are unique within the Dbl family insomuch as they encode two distinct DH/PH domain modules. (embl-heidelberg.de)

The extracellular domains interact in a homophilic manner to specify differential cell-cell connections. (nih.gov)

The DH domain is composed of 11 alpha helices that are folded into a flattened, elongated alpha-helix bundle in which two of the three conserved regions, conserved region 1 (CR1) and conserved region 3 (CR3), are exposed near the centre of one surface. (embl-heidelberg.de)

Epitope mapping showed that all Nbs recognize the Arc C-terminal region containing the retroviral Gag capsid homology domain, comprised of tandem N- and C-lobes. (kawasaki-m.ac.jp)

Anatomy18

Organisms11

Chemicals and Drugs104

Analytical, Diagnostic and Therapeutic Techniques and Equipment39

Phenomena and Processes82

Information Science4

Health Care1

Sequence homology4

Proteins22

Superfamily2

Repeats7

Regulates4

Sequences11

Phosphorylation5

Residues7

Motifs2

Actin6

Amino acids2

Intracellular7

Affinity1

Structurally4

Genome2

Structural4

Genes4

Ligand2

Subtypes1

Interaction1

Mediate1

Mutation1

Cysteine2

Lectin2

Complexes1

Recombinant1

Mediates1

Tail1

Crystal2

Share a conserved1

GTPase1

Peptide2

CrkL2

Mechanism1

Evidence1

Motif4

Transcription1

Human1

Sec71

Localization2

Highly expressed1

Family1

Homophilic1

Bundle1

Region1