• A mouse talin genomic clone (5Tλ2) was analyzed by restriction enzyme digestion and Southern blotting using oligonucleotide probes from the 5′ end of the talin cDNA. (nih.gov)
  • A) Gene structure of talin locus around the targeting site. (nih.gov)
  • It also allows cells to measure extracellular rigidity, since cells in which talin is prevented from forming mechanical linkages can no longer distinguish whether they are on a soft or rigid surface. (wikipedia.org)
  • In cells in which talin A was deleted, however, the furrow was wider and longer than in wildtype, and responded differently to mechanical stresses (in this case, being sandwiched tightly under a sheet of agarose), which appeared to be due to a disconnect between the actomyosin contractile ring and the plasma membrane. (phys.org)
  • Talin interacts with phosphatidylinositol-(4)-phosphate 5-kinase type Iγ, the major phosphatidylinositol-(4,5)-bisphosphate [PI(4,5)P 2 ]-synthesizing enzyme in brain. (rupress.org)
  • In these situations, talin codistributes with concentrations of integrins in the plasma membrane. (wikipedia.org)
  • Various whole cell extracts (30 µg) were separated by 5% SDS-PAGE, and the membrane was blotted with Talin-1 antibody [C C-term (GTX102215) diluted at 1:500. (genetex.com)
  • New work by Masatsune Tsujioka and colleagues in the Electron Microscope Laboratory reveals how a protein called talin A links the cytoskeletal complex actomyosin to the plasma membrane of Dictyostelium cells as they move and divide. (phys.org)
  • Talin recruitment to membrane. (mechanobio.info)
  • Activated Rap1 in turn, recruits Rap1-GTP-interacting adaptor molecule (RIAM) along with its binding partner, talin to the plasma membrane. (mechanobio.info)
  • When Tsujioka added talin A to Dictyostelium cells lacking both myosin II and endogenous talin, he found that it spread throughout the entirety of polarized migrating cells, rather than just in the rear, indicating that its distribution relies on myosin. (phys.org)
  • b) An x-y projection of endogenous talin. (nih.gov)
  • Furthermore, we demonstrate that disassembly of other adhesion components, including paxillin, vinculin and zyxin, is also dependent on the ability of calpain to cleave talin, suggesting a general role for talin proteolysis in regulating adhesion turnover. (nih.gov)
  • Interestingly, these differentiated talin (−/−) ES cells were able to spread and form focal adhesion-like structures containing vinculin and paxillin on fibronectin. (rupress.org)
  • Both talin (-/-) ES cell mutants formed embryoid bodies, but differentiation was restricted to two morphologically distinct cell types.Interestingly, these differentiated talin (-/-) ES cells were able to spread and form focal adhesion-like structures containing vinculin and paxillin on fibronectin.Moreover, the levels of the beta1 integrin subunit were comparable to those in wild-type ES cells. (nih.gov)
  • Localization of talin, vinculin, paxillin, and F-actin in differentiated talin (−/−) ES cell mutants by immunofluoresence. (nih.gov)
  • After 48 h, the cells were stained for talin and F-actin, vinculin and F-actin or paxillin and F-actin. (nih.gov)
  • Differentiated HM1 cells displayed a variety of morphologies, but all showed strong staining for talin, vinculin and paxillin in focal adhesions at the ends of actin filaments. (nih.gov)
  • The differentiated talin (−/−) ES cell mutants adopted a spread morphology with clear evidence of vinculin and paxillin-containing focal adhesion-like structures at the ends of actin filaments. (nih.gov)
  • However, in all cases there was prominent staining for talin, vinculin and paxillin at the ends of actin filaments (Fig. 9). (nih.gov)
  • Interestingly, the differentiated talin (−/−) A28 cells also contained actin filaments, and in some cells, the ends of these filaments showed staining for vinculin and paxillin, but not talin. (nih.gov)
  • Vinculin consists of a globular head domain that contains binding sites for talin and α-actinin as well as a tyrosine phosphorylation site, while the tail region contains binding sites for F-actin, paxillin, and lipids. (wikipedia.org)
  • In immunoblotting, the antibody recognizes an epitope located on the 225 kDa chicken talin molecule and the 190 kDa fragment obtained by protease cleavage. (genetex.com)
  • The molecule talin is thought to be the major initiator of vinculin activation due to its presence in focal complexes. (wikipedia.org)
  • Talin, a protein known to function in integrin-mediated cell-substrate adhesions, has been implicated as a candidate for this role, as loss of talin function has been shown in previous studies to affect bond between cells and their substrate. (phys.org)
  • A) Co-localization of talin A and myosin II during cell division. (phys.org)
  • He began by examining the subcellular localization of talin A (the Dictyostelium homolog of mammalian talins). (phys.org)
  • Finally, talin is a substrate for the calcium-ion activated protease, calpain II, which is also concentrated at points of cell-substratum contact. (wikipedia.org)
  • Here, we show that proteolysis of talin by the intracellular calcium-dependent protease calpain is critical for focal adhesion disassembly. (nih.gov)
  • We have generated a single point mutation in talin that renders it resistant to proteolysis by calpain. (nih.gov)
  • Quantification of adhesion assembly and disassembly rates demonstrates that calpain-mediated talin proteolysis is a rate-limiting step during adhesion turnover. (nih.gov)
  • Together, these findings identify calpain-mediated proteolysis of talin as a mechanism by which adhesion dynamics are regulated. (nih.gov)
  • RV pressure overload alters talin structure, probably through calpain activation. (ahajournals.org)
  • Calpain mediated talin disorganization may contribute to RV contractile dysfunction from acute RV pressure overload. (ahajournals.org)
  • Talin is also post-translationally modified via calpain 2-mediated cleavage, which may target it for ubiquitin-proteasome-mediated degradation and turnover of associated cell adhesion structures. (wikipedia.org)
  • In cultured cells, talin is absent from cell-cell junctions and found predominantly at adhesion plaques and in fibrillar streaks underlying cell surface fibronectin. (genetex.com)
  • Interestingly, the testis and kidney talin-2 isoforms lack the N-terminal 50% of the protein, and evidence suggests that this is the isoform expressed in elongating spermatids. (wikipedia.org)
  • Identification of a talin-binding site in the integrin beta(3) subunit distinct from the NPLY regulatory motif of post-ligand binding functions. (wikipedia.org)
  • He next used a sophisticated piece of kit called a total internal reflection fluorescence microscope, which enables very thin sections of a sample to be visualized, to check for direct binding between the two, but was intrigued to find that while talin A and myosin II remained close to one another, they were not directly connected. (phys.org)
  • As in migrating cells, talin co-localized with myosin II, in this case mid-cell near the cleavage furrow, and again talin's accumulation appeared to rely on myosin II. (phys.org)
  • For understanding the molecular and biophysical mechanism of rigidity sensing at cell-substrate adhesion sites, we constructed a simple physical model to examine a role of talin extension in the stiffness-dependent regulation of actin-integrin linkage. (royalsocietypublishing.org)