• CD45, previously called LCA (leukocyte common antigen), T200, or Ly5 in mouse, is member C of the class 1 (receptor-like) protein tyrosine phosphatase family (PTPRC) (1, 2). (rndsystems.com)
  • The OX-1 antibody recognizes all molecular forms of CD45 (Leukocyte Common Antigen) on all hematopoietic cells except erythrocytes. (bdbiosciences.com)
  • CD45 is a 180-240 kD glycoprotein also known as the leukocyte common antigen (LCA), T200, or Ly-5. (biolegend.com)
  • CD45 is a 180-240 kD single chain type I membrane glycoprotein also known as leukocyte common antigen (LCA) and T200. (biolegend.com)
  • The mouse monoclonal antibody HI30 recognizes an extracellular epitope on all isoforms of human CD45 antigen (Leukocyte Common Antigen), a 180-220 kDa single chain type I transmembrane protein expressed at high level on all cells of hematopoietic origin, except erythrocytes and platelets. (exbio.cz)
  • CD45 (LCA, leukocyte common antigen) is a receptor-type protein tyrosine phosphatase ubiquitously expressed in all nucleated hematopoietic cells, comprising approximately 10% of all surface proteins in lymphocytes. (exbio.cz)
  • Leukocyte common antigen precursor (EC 3.1.3.48) (L-CA) (CD45 antigen) (T200). (lu.se)
  • Evidence from cDNA clones that the rat leukocyte-common antigen (T200) spans the lipid bilayer and contains a cytoplasmic domain of 80,000 Mr". Cell. (wikipedia.org)
  • Clark E A and Ledbetter J A. Leukocyte cell surface enzymology: CD45 (LCA, T200) is a protein tyrosine phosphatase. (histoprime.com)
  • The CD45 antigen (Receptor-type tyrosine-protein phosphatase C, PTPRC) is a protein tyrosine-protein phosphatase required for T-cell activation through the antigen receptor. (otwobio.com)
  • CD45R, also designated CD45 and PTPRC, has been identified as a transmembrane glycoprotein, broadly expressed among hematopoietic cells. (abeomics.com)
  • CD45 has been best studied in T cells, where it determines T cell receptor signaling thresholds (3, 6‑8). (rndsystems.com)
  • The CD45 isoforms play complex roles in T-cell and B-cell antigen receptor signal transduction. (bdbiosciences.com)
  • CD45 plays a critical role in T and B cell antigen receptor-mediated activation by dephosphorylating substrates including p56Lck, p59Fyn, and other Src family kinases. (biolegend.com)
  • van den Berg HA, Wooldridge L, Laugel B, Sewell AK: Coreceptor CD8-driven modulation of T cell antigen receptor specificity. (exbio.cz)
  • This PTP has been shown to be an essential regulator of T- and B-cell antigen receptor signaling. (origene.com)
  • It functions through either direct interaction with components of the antigen receptor complexes, or by activating various Src family kinases required for the antigen receptor signaling. (origene.com)
  • Evidence for the T3-associated 90k heterodimer as the T cell antigen receptor. (histoprime.com)
  • Immunohistochemical staining of rat leukocytes: Zinc-fixed paraffin sections of normal rat intestine were reacted with the anti-CD45 antibody. (bdbiosciences.com)
  • The OX-1 antibody specific for rat CD45 is recommended to test for immunohistochemical staining of Zinc-fixed paraffin sections. (bdbiosciences.com)
  • The CD45 antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. (biolegend.com)
  • Antibody to CD45 is useful in differential diagnosis of lymphoid tumors from non-hematopoietic undifferentiated neoplasms. (abeomics.com)
  • The non-restricted CD45, Leucocyte Common Antigen (LCA) consists of an extracellular sequence, proximal to the membrane, which is common to all CD45 isoforms. (beckman.com)
  • Recognizes all CD45 isoforms as well as the known mouse CD45 alloantigens (CD45-1/Ly 5a and CD45-2/Ly 5b) (15). (rndsystems.com)
  • CD45 has several isoforms, expressed according to cell type, developmental stage and antigenic exposure (1‑5). (rndsystems.com)
  • The CD45 isoforms detected in rat are cell type-, maturation-, and activation state-specific. (bdbiosciences.com)
  • The CD45 molecules are single chain integral membrane proteins comprising at least five isoforms ranging from 180 to 220 kDa. (beckman.com)
  • All the monoclonal antibodies that belong to the CD45 cluster react with this part of the antigen and are able to recognize all CD45 isoforms. (beckman.com)
  • CD45 antibodies react with leucocyte progenitors in bone marrow. (beckman.com)
  • The antibodies are specific against mouse CD45 and MHC class I (of a, b, d, j, k, s, and u haplotypes) and can be used to label hematopoietic and non-hematopoietic cells in most commonly used mouse strains for multiplex single cell sequencing analysis. (biolegend.com)
  • Following this his group generated antibodies that identified rat CD8, a marker of cytotoxic T cells as well as rat CD45. (wikipedia.org)
  • A second signal for T cell mitogenesis provided by monoclonal antibodies CD45 (T200). (absoluteantibody.com)
  • CD45 glycoprotein is crucial in lymphocyte development and antigen signaling, serving as an important regulator of Src-family kinases. (exbio.cz)
  • In other leukocytes, CD45 influences differentiation and links immunoreceptor signaling with cytokine secretion and cell survival, partially overlapping in function with DEP-1/CD148 (11‑14). (rndsystems.com)
  • CD45 is a signaling molecule that regulates a variety of cellular processes including cell growth, differentiation, cell cycle, and oncogenic transformation. (biolegend.com)
  • Leucocyte Typing III, White Cell Differentiation Antigens. (histoprime.com)
  • The non-restricted CD45 epitope is present on the surface of all human leucocytes, lymphocytes, eosinophils monocytes, basophils and neutrophils by order of decreasing level of expression. (beckman.com)
  • CD45 non-covalently associates with lymphocyte phosphatase-associated phosphoprotein (LPAP) on T and B lymphocytes. (biolegend.com)
  • Besides the role in immunoreceptor signaling, CD45 is important in promoting cell survival by modulating integrin-mediated signal transduction pathway and is also involved in DNA fragmentation during apoptosis. (exbio.cz)
  • Importantly, some cell lines or experimental models may lack or express very low levels of either or both CD45 and MHC I molecules. (biolegend.com)
  • The CD3- molecules are involved in signal transduction after antigen binding to the TCR. (histoprime.com)
  • CD45 acts as a positive regulator of T-cell coactivation upon binding to DPP4. (otwobio.com)
  • MHC class I is involved in antigen presentation to T cells expressing CD3/TCR and CD8 proteins. (biolegend.com)
  • CD45 is moved into or out of the immunological synapse (IS) membrane microdomain depending on the relative influence of interaction with the extracellular galectin lattice or the intracellular actin cytoskeleton (9, 10). (rndsystems.com)
  • Additional reported applications (for the relevant formats) include: immunohistochemical staining of acetone-fixed frozen tissue sections and formalin-fixed paraffin-embedded tissue sections 9 , inhibition of CD45 functions 4 , immunofluorescence 11 , Western blotting 3 , and spatial biology (IBEX) 16,17 . (biolegend.com)
  • Formalin-fixed, paraffin-embedded human Tonsil stained with CD45 Ab (2B11). (abeomics.com)
  • The cytoplasmic expression of CD3 antigens in normal and malignant cells of the T lymphoid lineage. (histoprime.com)
  • Leukocytes expressing CD45 can be identified by the brown labeling of their cell membranes. (bdbiosciences.com)
  • Within the IS, CD45 dephosphorylates and negatively regulates the src family kinase, LCK (8‑10). (rndsystems.com)
  • Cosenza-Nashat MA, Kim MO, Zhao ML, Suh HS, Lee SC: CD45 isoform expression in microglia and inflammatory cells in HIV-1 encephalitis. (exbio.cz)
  • CD45 deletion causes in severe immunodeficiency, while point mutations may be associated with autoimmune disorders (6, 7). (rndsystems.com)
  • Defects in CD45 are a cause of severe combined immunodefiency autosomal recessive T-cell negative/B-cell-positive/NK-cell-positive (T-B+NK+ SCID). (otwobio.com)
  • CD45 has been reported to bind galectin-1 and to be associated with several other cell surface antigens including CD1, CD2, CD3, and CD4. (biolegend.com)
  • Genetic variations in CD45 are involved in multiple sclerosis susceptibility. (otwobio.com)
  • CD45 is a major component of the lymphocyte membrane. (beckman.com)