Here we present CRISPR- and RNA-assisted in vivo directed evolution (CRAIDE) of genomic loci using evolving chimeric donor gRNAs continuously delivered from an error-prone T7 RNA polymerase, and directly introduced as RNA repair donors into genomic targets under either Cas9 or dCas9 guidance. (escholarship.org)
Review of zinc as an inhibitor of SARS-CoV-2′s RNA-dependent RNA polymerase, and zinc ionophores including CQ/HCQ, showing the latest evidence for zinc and CQ/HCQ having antiviral, and in particular anticoronaviral action. (c19hcq.org)
Zn(II) is an inhibitor of SARS-CoV-2 s RNA-dependent RNA polymerase, and chloroquine and hydroxychloroquine are Zn(II) ionophores-this statement gives a curious mind a lot to think about. (c19hcq.org)
Proteins10
Similarly to the studies carried out two decades ago for proteins, which gave the fundamental grounds for developing comparative protein structure prediction methods, we are now able to quantify the relationship between sequence and structure conservation in RNA. (biomedcentral.com)
Similar to proteins, functional RNA molecules fold into specific three-dimensional conformations essential for performing their biological activity. (biomedcentral.com)
Nonetheless, it is common knowledge that RNA 3D structure is more conserved than RNA sequence and that such principle could be used for comparative RNA structure prediction in a similar way it is done for proteins [ 11 ]. (biomedcentral.com)
F o For as long as living things have been building proteins based on the code of messenger RNA molecules, there have been aminoacyl-tRNA synthetases. (fascea.pics)
We insert synthetic peptides into proteins of interest via tandem protein trans-splicing using two orthogonal split intein pairs and validate our approach by investigating different aspects of GFP, Na V 1.5 and P2X2 receptor function. (biorxiv.org)
Because the approach can introduce virtually any chemical modification into both intracellular and extracellular regions of target proteins, we anticipate that it will overcome some of the drawbacks of other semi-synthetic or ribosome-dependent methods to engineer proteins. (biorxiv.org)
Semi-synthetic approaches offer an alternative means to manipulate proteins post-translationally, but these modifications have typically been performed in vitro 3 - 8 . (biorxiv.org)
We thus sought to complement these approaches with a method that could incorporate synthetic peptides carrying multiple post-translational modifications (PTMs) or ncAAs into both cytosolic and membrane proteins in live eukaryotic cells. (biorxiv.org)
TM84 requires tRNA(Leu) for tight binding to the LeuRS synthetic active site, unlike any previously reported inhibitors. (ox.ac.uk)
He received his Ph.D. degree in the laboratory of Dr. En-Duo Wang in Biochemistry and Molecular Biology from Shanghai Institute of Biochemistry and Cell Biology of Chinese Academy of Science in 2008, where he studied the structural and functional interplay of transfer RNA (tRNA) and tRNA synthetase in bacteria, yeast and human. (rochester.edu)
Synthetase1
Expanding the substrate scope of pyrrolysyl-transfer RNA synthetase enzymes to include non-σ-amino acids in vitro and in vivo. (harvard.edu)
Biology3
Our new modules enable ligand-programmable multi-channel CID systems for plant and eukaryotic synthetic biology that can empower new plant-based and microbe-based sensing modalities. (nature.com)
In recent years, the number of available RNA structures has rapidly grown reflecting the increased interest on RNA biology. (biomedcentral.com)
Rapid advances in synthetic biology are driving the development of genetically engineered microbes as therapeutic agents for a multitude of human diseases, including cancer. (nature.com)
Tertiary1
Finally, with the aim of characterizing RNA structure diversity, Abraham and co-workers recently studied the RNA conservation at three levels: primary, secondary, and tertiary structure. (biomedcentral.com)
Peptide1
The sequence of this synthetic peptide is based on the primary site of cleavage by plasmepsin II (PM II) within hemoglobin (Hb), and numbering of the peptide corresponds to the residue within the α-chain of Hb. (eurogentec.com)
Control gene expression2
The long-term objective of our laboratory is to identify novel RNA-based molecular mechanisms that control gene expression and conduct pathophysiological function in cardiac system, as well as to develop novel therapeutic approaches for the prevention or treatment of human cardiovascular diseases. (rochester.edu)
It uses a fusion protein, consisting of a catalytically impaired Cas9 endonuclease fused to an engineered reverse transcriptase enzyme, and a prime editing guide RNA (pegRNA), capable of identifying the target site and providing the new genetic information to replace the target DNAnucleotides. (wikipedia.org)
A single guide RNA (sgRNA) that directs the Cas9 H840A nickase portion of the fusion protein to nick the non-edited DNA strand. (wikipedia.org)
We recently discovered a new type of stress-responsive, protein-directed human RNA switch that regulates expression of vascular endothelial growth factor-A in human monocytic cells (Ray, PS, et al. (rochester.edu)
Intriguingly, some of these RNA switches involve the interplay between microRNA and RNA-binding protein (Jafarifar, F, et al. (rochester.edu)
One of the microRNA strands of the duplex is loaded onto the RNA-induced silencing complex (RISC) where it is then able to either cleave RNA targets or repress protein translation dependent upon its complementarity to the target mRNA. (biomedcentral.com)
Among these are energy-consuming enzymes such as GTPases, protein kinases, ATP-dependent RNA helicases, and AAA-type (ATPases associated with various cellular activities) ATPases, which suggests that the energy derived from nucleotide hydrolysis confers directionality to ribosome assembly. (silverchair.com)
Functional1
MicroRNAs are a group of small noncoding functional RNAs that are approximately 22 nucleotides in length [ 1 ]. (biomedcentral.com)
Homology1
The computational analysis here presented quantitatively describes the relationship between sequence and structure for RNA molecules and defines a twilight zone region for detecting RNA homology. (biomedcentral.com)
Molecules3
RNA molecules are now known to carry a large repertory of biological functions such as transfer of information, enzymatic catalysis and regulation of cellular processes [ 4 ]. (biomedcentral.com)
However, to date no general large-scale study has systematically addressed the quantitative analysis of the relationship between sequence and structure conservation in RNA molecules. (biomedcentral.com)
But just as it had originally been the case in other fields of synthetic chemistry, such as molecular or solid state chemistry, the outcome of such a deposition with respect to the structure of the individual molecule or the structural arrangement of many molecules is very hard to predict without prior experimental input. (degruyter.com)
Eukaryotic1
Despite recent progress in ribosome-dependent and semi-synthetic chemical modifications, these techniques sometimes have limitations in the number and type of modifications that can be simultaneously introduced or their application in live eukaryotic cells. (biorxiv.org)
Enzymes1
These enzymes, abbreviated as AARS, link transfer RNAs (tRNAs) with the corresponding amino acids. (fascea.pics)
Prediction1
Our work could represent the theoretical basis and limitations for future developments in comparative RNA 3D structure prediction. (biomedcentral.com)
Sequence2
Prime editing involves three major components: A prime editing guide RNA (pegRNA), capable of (i) identifying the target nucleotide sequence to be edited, and (ii) encoding new genetic information that replaces the targeted sequence. (wikipedia.org)
The pegRNA consists of an extended single guide RNA (sgRNA) containing a primer binding site (PBS) and a reverse transcriptase (RT) template sequence. (wikipedia.org)
Transcript2
Transcript evaluation Complete RNA was isolated from fresh blood with QIAamp RNA blood kit, and single stranded cDNA was synthesized together with the GoScript reverse transcription procedure in ac cordance using the companies directions. (mirnainhibitors.com)
Our laboratory is interested in the understanding of pathophysiological function and molecular mechanism of new non-coding RNAs (and RBPs) and new modes of gene regulation in c. (rochester.edu)
After aligning the octopamine gene isoforms using Clustal Omega ( https://www.ebi.ac.uk/Tools/msa/clustalo/ ), conserved regions were used for siRNA design using Dharmacon's siDESIGN tool ( https://horizondiscovery.com/en/products/tools/siDESIGN-Center ). (biomedcentral.com)
Amino1
In order to enhance DNA-RNA affinity, enzyme processivity, and thermostability, five amino acid substitutions were incorporated into the M-MLV reverse transcriptase. (wikipedia.org)
Yeast1
A synthetic RNA-mediated evolution system in yeast. (escholarship.org)
Chemistry1
Throughout its history, the field of synthetic chemistry has been divided into several subfields, depending on the way chemistry has been perceived by its practitioners. (degruyter.com)
Biological1
Alfonso, Quesada, Biological activity of synthetic ionophores: Ion transporters as prospective drugs? (c19hcq.org)
System1
To establish this holly purpose the system uses the gRNA- guided RNA which leads the nuclease (CAS) to a specific location. (geneticeducation.co.in)
M-MLV reverse transcriptase: an enzyme that synthesizes DNA from a single-stranded RNA template. (wikipedia.org)
To avoid this problem during DNA heteroduplex resolution, an additional single guide RNA (sgRNA) is introduced. (wikipedia.org)
We generated a single-cell RNA sequencing map of the dynamics of human macrophage specification from PCW 4-26 across 19 tissues. (bvsalud.org)
Development1
These small RNAs have been shown to contribute to the control of cell growth, differentiation and apoptosis, important features related to cancer development and progression. (biomedcentral.com)
Represent1
Messenger RNA (mRNA) vaccines represent a relatively new vaccine class showing great promise for the future. (researchgate.net)
Method1
A new method that relies on secondary structure information to align homolog RNA sequences was also recently developed [ 49 ]. (biomedcentral.com)
Main1
There were two main goals: (1) To determine whether feeding synthetic dsRNA to D. arcuata larvae would elicit an RNAi response. (biomedcentral.com)
Activity1
Synthetic rat adrenomedullin (rADM) induces effective and durable antihypertensive activity in anesthetized rats. (bestbiochem.com)