• BirA, the biotin-protein ligase (BPL) of Escherichia coli, is also known as biotin operon repressor, biotin-[acetyl-CoA-carboxylase] ligase, and biotin-[acetyl-CoAcarboxylase] synthetase. (watson-int.cn)
  • Acetyl-CoA carboxylase (ACC) is a biotin-dependent enzyme. (pberghei.eu)
  • 31785) acetyl-CoA carboxylase%2C biotin carboxylase CP001857 CDS Arcpr_0038 complement(31809. (go.jp)
  • Reliable markers for biotin status include the activity of propionyl-CoA carboxylase in lymphocytes, and the urinary excretion of biotin and the metabolite 3-hydroxyisovaleric acid. (nebraska.edu)
  • Regardless of how biotin is acquired, a dedicated ATP-dependent biotin ligase, or holocarboxylase synthetase (HCS), is required to catalyze the covalent attachment of biotin to a specific lysine in the target carboxylase. (pberghei.eu)
  • Phosphoribosylglycinamide synthetase ( EC 6.3.4.13 ) (GARS) (phosphoribosylamine glycine ligase) [ ( PUBMED:2687276 ) ] catalyses the second step in the de novo biosynthesis of purine. (embl.de)
  • BirA functions both as a DNA-binding protein that represses the biotin biosynthesis operon as well as an enzyme that synthesizes its own corepressor, bio-5′-AMP, an intermediate in biotinylation reactions. (watson-int.cn)
  • utilized tetracycline-regulatable promoter components to create mycobacterial strains that conditionally exhibit pantothenate synthetase (knockdown stress in conjunction with a fluorescence displacement assay to recognize hits that particularly focus on biotin biosynthesis (19). (icsv20.org)
  • Patients seemed to respond to biotin administration, and each affected enzyme required biotin as a cofactor. (medscape.com)
  • The major contribution of Wolf et al was in demonstrating the presence of the enzyme human biotinidase and its role in biotin recycling. (medscape.com)
  • Description: This is Double-antibody Sandwich Enzyme-linked immunosorbent assay for detection of Human Glycyl tRNA Synthetase (GARS) in serum, plasma, tissue homogenates and other biological fluids. (hudsen.org)
  • Description: Enzyme-linked immunosorbent assay based on the Double-antibody Sandwich method for detection of Human Glycyl tRNA Synthetase (GARS) in samples from serum, plasma, tissue homogenates and other biological fluids with no significant corss-reactivity with analogues from other species. (hudsen.org)
  • also at this step, N-acetylglutamate exerts its regulatory control on the mediating enzyme, carbamyl phosphate synthetase (CPS). (medscape.com)
  • Vitamin B12 (B12) is an essential cofactor for two enzymes involved in one-carbon metabolism: methylmalonyl CoA mutase (reduced function of this enzyme results in increased serum methylmalonic acid (MMA) levels) and methionine synthetase (this enzyme catalyzes the remethylmation of homocysteine to methionine) (IOM 1998). (cdc.gov)
  • A particular allelic variant, the p.L216R mutation, results in an unstable protein with increased turnover and a clinical picture of relative biotin-resistance. (medscape.com)
  • Biotin is also necessary to produce keratin, a protein that promotes strong nails and hair. (healthline.com)
  • The protein encoded by this gene belongs to the biotin and lipoic acid synthetases family. (abnova.com)
  • The assay for holocarboxylase synthetase activity therefore requires a protein apocarboxylase substrate which is not readily available. (elsevierpure.com)
  • Avidin, an egg protein, makes a complex with biotin and make it unavailable for human use. (beautyhealthtips.in)
  • BirA, a member of the group II biotin-protein ligase family, contains an N-terminal helix-turn-helix DNA-binding domain, a catalytic core that catalyzes biotinyl 5′ adenylate (bio-5′-AMP) synthesis, and a C-terminal domain that plays a role in DNA binding, dimerization, and catalytic function. (watson-int.cn)
  • BirA biotinylates via the lysine side chain of biotin-accepting proteins/peptides, including natural substrate, carboxyl carrier protein (BCCP),and Avi Tag fusion proteins. (watson-int.cn)
  • Once biotinylated, (strept)avidin-biotin interactions can be utilized in a wide variety of applications of biochemistry and cell biology, including protein capture, immobilization, multimerizing, and bridging molecules. (watson-int.cn)
  • Anticonvulsants and lipoic acid may interfere with biotin metabolism, thereby increasing biotin requirements. (nebraska.edu)
  • Through this carboxyl group, biotin is linked covalently to the β-amino group of lysine in 4 carboxylases that play critical roles in intermediary metabolism. (medscape.com)
  • It is also known as Vitamin H. Biotin mediates a wide variety of chemical reactions inside the body ranging from metabolism of fats, amino acids, and carbohydrates. (beautyhealthtips.in)
  • Biotin carboxylases participate in central metabolic processes such as gluconeogenesis, lipogenesis, amino acid metabolism, and energy transduction. (pberghei.eu)
  • Perhaps the most unusual feature of biotin metabolism in malaria parasites is the presence of two HCS paralogs (HCS1, HCS2) encoded in the genomes of Plasmodium species. (pberghei.eu)
  • In all 4 carboxylases, biotin functions as a coenzyme or prosthetic group that serves as a carrier for CO 2 in a multistep reaction. (medscape.com)
  • It functions as a CO2 carrier in carboxylation and decarboxylation reactions catalyzed by a small family of enzymes, the biotin carboxylases. (pberghei.eu)
  • Holocarboxylase synthetase, biotinidase, sodium-dependent multivitamin transporter, and the biotin transporters SMVT and MCT1 play crucial roles in biotin homeostasis in mammals. (nebraska.edu)
  • Biotin is also sometimes called B-7, vitamin H, or coenzyme R. (healthline.com)
  • Biotin is the vitamin which is used as a coenzyme to assist in various metabolic reactions which are essential for the development of healthy hair. (beautyhealthtips.in)
  • During the 3rd incubation, streptavidin-coated microparticles and vitamin B 12 labeled with biotin are added and the still-vacant sites of the ruthenium labeled intrinsic factor become occupied. (cdc.gov)
  • This forms a ruthenium labeled intrinsic factor-vitamin B 12 biotin complex. (cdc.gov)
  • Biotin also regulates gene expression, mediated by biotinylation of lysine residues in histones H2A, H3, and H4, and by various transcription factors. (nebraska.edu)
  • Description: A sandwich quantitative ELISA assay kit for detection of Human Glycyl tRNA Synthetase (GARS) in samples from serum, plasma, tissue homogenates or other biological fluids. (hudsen.org)
  • Description: A sandwich ELISA kit for detection of Lysyl tRNA Synthetase from Human in samples from blood, serum, plasma, cell culture fluid and other biological fluids. (jemsec.com)
  • tRNA synthetase class II core domain (G, Seryl-tRNA synthetase N-terminal domain [Interproscan]. (ntu.edu.sg)
  • 4560 valyl-tRNA synthetase CP001857 CDS Arcpr_0004 4868. (go.jp)
  • 55056) tyrosyl-tRNA synthetase CP001857 CDS Arcpr_0065 complement(55112. (go.jp)
  • This directed attention toward the work of Lynen, who had previously demonstrated that carbon dioxide fixation in microbes required covalent attachment of the biotin to an apoprotein. (medscape.com)
  • Notable differences included the age at presentation (neonatal to 9 mo), presence of acidosis, and response to biotin administration. (medscape.com)
  • Sweetman (1981) proposed a defect in holocarboxylase synthetase and intestinal biotin absorption, respectively. (beds.ac.uk)
  • Third, the intestinal flora synthesizes significant quantities of biotin, and at least a portion of that biotin is believed to be absorbed into the bloodstream. (medscape.com)
  • Evidence regarding the interactions between the drugs carbamazepine, primidone and phenytoin and the nutrients biotin and pantothenate concluded they (prescribed medications) specifically and competitively inhibit biotin absorption via intestinal brush border membranes (Said et al, 1989), and that there is competitive inhibition of absorption between carbamazepine, biotin and pantothenic acid (Said, 1999). (medicationsandnutrition.online)
  • Individuals carrying mutations in genes coding for holocarboxylase synthetase and biotinidase require lifelong supplementation with pharmacological doses of biotin. (nebraska.edu)
  • Synergistic effect of shear stress and streptavidin-biotin on the expression of endothelial vasodilator and cytoskeleton genes. (duke.edu)
  • The results suggest (1) the use of SA-biotin to supplement EC adhesion enhances the integrity of the EC cytoskeleton by upregulating the expression of cytoskeleton/ECM genes, and (2) a likely relationship between the expression of cytoskeleton/ECM genes and the downstream events, such as the shear-induced expression of eNOS and COX2 genes. (duke.edu)
  • Dual ligand treatment of streptavidin(SA)-biotin and fibronectin (Fn) enhances the adhesion of endothelial cells (EC) onto synthetic surfaces and promotes the quiescent phenotype of adherent EC. (duke.edu)
  • The entire complex becomes bound to the solid phase via interaction of biotin and streptavidin. (cdc.gov)
  • Various studies have been conducted to demonstrate the efficacy of biotin supplementation on hair health but most of them failed to conclude a favorable result. (beautyhealthtips.in)
  • In these cases, apart from the biotin supplementation, various foods are incorporated in the diet of patients. (beautyhealthtips.in)
  • Biotin is an essential metabolite in all known free-living organisms. (pberghei.eu)
  • Holocarboxylase synthetase first catalyzes the formation of biotinyl-AMP from biotin and ATP, an activity designated as biotinyl-AMP synthetase. (elsevierpure.com)
  • Catalyzes the conversion of dethiobiotin to biotin. (or.jp)
  • Holocarboxylase Synthetase: A Moonlighting Transcriptional Coregulator of Gene Expression and a Cytosolic Regulator of Biotin Utilization. (medscape.com)
  • Analyses presented in this study provide insights into the mechanism by which SA-biotin-supplemented EC mediate gene expression. (duke.edu)
  • Biotin is synthesized by the bacteria present in the intestine. (beautyhealthtips.in)
  • Biotin is produced in the body by the intestinal bacteria. (beautyhealthtips.in)
  • It is to be noted that the biotin produced through intestinal bacteria is sufficient to cater body needs. (beautyhealthtips.in)
  • Bacteria, plants, and some fungi can synthesize biotin de novo from a pimeloyl-CoA precursor, but other organisms acquire biotin from their diet or environment. (pberghei.eu)
  • An important reaction related to the growth of hair and nails assisted by biotin is the gluconeogenesis. (beautyhealthtips.in)
  • This child died 105 hours postdelivery after little improvement from small intravenous doses of megavitamins, including biotin. (medscape.com)
  • Both directly and via its various metabolites, chronic alcohol exposure has been found to cause significant inhibition in both SMVT biotin uptake of the microbiota-generated biotin, and the degree of expression of SMVT. (medicationsandnutrition.online)
  • These levels are elevated due to impairment of renal reclamation of biotin. (loinc.org)
  • At the end of the reaction, unreacted radioactive biotin substrate, which is negatively charged at neutral pH, is bound to an anion-exchange resin and a neutral radioactive biotinylhydroxamate product in the supernatant is counted. (elsevierpure.com)
  • The early form showed higher urinary excretion of 3-hydroxyisovaleric acid and 3-hydroxypropionic acid than the late form and was associated with normal plasma biotin concentrations. (beds.ac.uk)
  • the sibling had an excellent clinical response to biotin administration in pharmacologic doses. (medscape.com)