Synthesis of peptide polyketide peptide synthases. Medical search. Frequent questions

Finally, combined quantum mechanical/molecular mechanical (QM/MM) modelling of the IdmH reaction shows that the active site of the enzyme provides an appropriate environment to promote indane-ring formation, supporting the assignment of IdmH as the key Diels-Alderase catalysing the final step in the biosynthesis of indanomycin through a similar mechanism to other recently characterized Diels-Alderases involved in polyketide-tailoring reactions. (iucr.org)
During iron starvation, HapX represses iron-consuming pathways, including heme biosynthesis and respiration, to spare iron and activates synthesis of ribotoxin AspF1 and siderophores, the latter partly by ensuring supply of the precursor, ornithine. (frontiersin.org)
Antitumor polyketide biosynthesis by an uncultivated bacterial symbiont of the marine sponge Theonella swinhoei. (mpg.de)
Cloning, sequencing and analysis of the enterocin biosynthesis gene cluster from the marine isolate 'Streptomyces maritimus': evidence for the derailment of an aromatic polyketide synthase. (mpg.de)
This study describes new gamma-lactam and related metabolites at the intersection of colibactin and fatty acid biosynthesis, establishing a new form of "cross-talk" between secondary polyketide and primary fatty acid pathways. (yale.edu)
Tang X, Eitel K, Kaysser L, Kulik A, Grond S, Gust B . (2013) A two-step sulfation in antibiotic biosynthesis requires a type III polyketide synthase. (uni-tuebingen.de)
Biosynthesis of Vitroprocines by α-Oxoamine Synthase and Oxidoreductase Identified from Vibrio sp. (tmu.edu.tw)
Fungal polyketide biosynthesis - a personal perspective. (shengsci.com)
This Viewpoint article provides a personal viewpoint of research in the area of fungal polyketide biosynthesis. (shengsci.com)
Biosynthesis of polyketides by trans-AT polyketide synthases. (shengsci.com)
This review discusses the biosynthesis of natural products that are generated by trans-AT polyketide synthases, a family of catalytically versatile enzymes that represents one of the major group of proteins involved in the production of bioactive polyketides. (shengsci.com)

Indanomycin is biosynthesized by a hybrid nonribosomal peptide synthase/polyketide synthase (NRPS/PKS) followed by a number of `tailoring' steps to form the two ring systems that are present in the mature product. (iucr.org)
Natural products classed as polyketides, nonribosomal peptides and hybrids of both are often biosynthesized by giant, complex enzymes known as polyketide synthases (PKS) and nonribosomal peptide synthetases (NRPS). (iucr.org)
Ybt synthesis occurs by a mixed nonribosomal peptide synthetase (NRPS)/polyketide synthase (PKS) mechanism. (wikipedia.org)
While there exist a wide range of peptides that are not synthesized by ribosomes, the term nonribosomal peptide typically refers to a very specific set of these as discussed in this article. (wikimili.com)

Polyketide genes in the marine sponge Plakortis simplex: a new group of mono-modular type I polyketide synthases from sponge symbionts. (cirn-na.com)
Engineering biodiversity with type II polyketide synthase genes. (mpg.de)
Many functional genes affect metabolite synthesis, metabolism, and regulation. (biomedcentral.com)
At the same time, the metabolic phenotype was used for genome-wide association analysis to analyze its genetic model and identify genes related to metabolite synthesis and metabolic pathways. (biomedcentral.com)

The applicability of different enzymes such as glycosidases, glycosyltransferases, glycophosphorylases and glycosynthases have been de-scribed around the review article considering their advantages and disadvantages of these biocat-alysts in the stereoselective and regioselective synthesis of different types of glycosylated mole-cules, phenolic and aliphatic alcohols, oligosaccharides, polysaccharides, glycoderivatives, glyco-peptides and glycoproteins with a clear focus on food and pharmaceutical chemistry. (preprints.org)

A polyketide synthase-peptide synthetase gene cluster from an uncultured bacterial symbiont of Paederus beetles. (mpg.de)

Screening of the metagenome of the marine sponge Plakortis simplex led to the discovery of the swf family, a new group of mono-modular type I polyketide synthase/fatty acid synthase (PKS/FAS) specifically associated with sponge symbionts. (cirn-na.com)

Smenamides A and B, chlorinated peptide/polyketide hybrids containing a dolapyrrolidinone unit from the Caribbean sponge Smenospongia aurea. (cirn-na.com)

Unprecedented diversity of catalytic domains in the first four modules of the putative pederin polyketide synthase. (mpg.de)

This technique has frequently been applied to animal tissue slices for the purpose to map proteins, peptides , lipids, sugars or small metabolites to find disease specific bio-markers or to study drag metabolism. (go.jp)
This cupin signature is found as a central component of many microbial proteins including certain types of phosphomannose isomerase, polyketide synthase, epimerase, and dioxygenase. (embl-heidelberg.de)

Many of these functions involve aspects of sugar metabolism and cell wall synthesis and are concerned with responses to abiotic stress such as heat, desiccation, or starvation. (embl-heidelberg.de)
This study establishes the biochemical function of LACC1 in humans/mice and its role in bridging inducible nitric oxide synthase and polyamine metabolism in inflammatory macrophages. (yale.edu)

Identification of Novel Coumestan Derivatives as Polyketide Synthase 13 Inhibitors against Mycobacterium tuberculosis. (inra.fr)

Acyltransferase Domain Exchange between Two Independent Type I Polyketide Synthases in the Same Producer Strain of Macrolide Antibiotics. (go.jp)
2014) Mechanism of action of the uridyl peptide antibiotics: an unexpected link to a protein-protein interaction site in translocase MraY. (uni-tuebingen.de)

Exploring the chemistry of uncultivated bacterial symbionts: Antitumor polyketides of the pederin family. (mpg.de)

The synthesis of four novel platinum complexes, bearing N6-(6-amino-hexyl)adenosine or a 1,6-di(adenosin-N6-yl)-hexane respectively, as ligands of mono-functional cisplatin or monochloro(ethylendiamine)platinum(II), is reported. (cirn-na.com)
A novel Dps-type protein from insect gut bacteria catalyses hydrolysis and synthesis of N-acyl amino acids. (mpg.de)

Targeting modular polyketide synthases with iteratively acting acyltransferases from metagenomes of uncultured bacterial consortia. (mpg.de)

Nonribosomal peptides ( NRP ) are a class of peptide secondary metabolites, usually produced by microorganisms like bacteria and fungi. (wikimili.com)

Nonribosomal peptides are also found in higher organisms, such as nudibranchs, but are thought to be made by bacteria inside these organisms. (wikimili.com)

Linear and cyclic peptides from the entomopathogenic bacterium Xenorhabdus nematophilus. (secondarymetabolites.org)

Intermediates are bound to the megasynthase during synthesis until they are released in the final step as mature natural compound. (proloewe.de)

Synthesis and pharmacological evaluation of modified adenosines joined to mono-functional platinum moieties. (cirn-na.com)

124 peptides have been observed through mass spectrometry data. (wikidoc.org)

The accumulation of both peptides in the endothelial cells suggested in vivo inhibitory activity of the angiotensin I-converting enzyme. (go.jp)

Furthermore, the Corramycin biosynthetic gene cluster (BGC) was identified and a biosynthesis model was proposed involving a 12-modular non-ribosomal peptide synthetase/polyketide synthase. (bvsalud.org)
In his research on the topic of Biosynthesis, Peptide bond is strongly related with Stereochemistry. (research.com)
Roderich D. Süssmuth spends much of his time researching Biochemistry, Peptide, Biosynthesis, Nonribosomal peptide and Venom. (research.com)
My team has accumulated a good body of work on bioactive natural product discovery (from polyketides and peptides to alkaloids) and their biosynthesis, and new enzyme investigation as shown below. (abdn.ac.uk)
The biosynthesis of various types of natural products such as polyketides, non-ribosomal peptides and terpenoids is based on the core enzymes polyketide synthase (PKS), non-ribosomal peptide synthase (NRPS), and terpene synthase (TS). (springernature.com)
The thioesterase (TE)-mediated macrocyclization is regarded as the most canonical strategy in polyketide and nonribosomal peptide biosynthesis, where a hydroxyl or an amino group in the polyketide or peptide acyl chain acts as an internal nucleophile to attack the TE-tethered carbonyl carbon, generating a macrolactone or a macrolactam. (springernature.com)
a dual functional enzyme, LkcE, catalyzes a unique amide oxidation and a subsequent Mannich reaction to form the polyketide macrocycle in the lankacidin biosynthesis. (springernature.com)
6. Oxazolomycin biosynthesis in Streptomyces albus JA3453 featuring an "acyltransferase-less" type I polyketide synthase that incorporates two distinct extender units. (nih.gov)
10. The biosynthetic gene cluster for the anticancer drug bleomycin from Streptomyces verticillus ATCC15003 as a model for hybrid peptide-polyketide natural product biosynthesis. (nih.gov)
13. Cloning, sequencing and analysis of the enterocin biosynthesis gene cluster from the marine isolate 'Streptomyces maritimus': evidence for the derailment of an aromatic polyketide synthase. (nih.gov)
ascomyceticus (ATCC 14891) contains genes for biosynthesis of unusual polyketide extender units. (nih.gov)
19. Leinamycin biosynthesis revealing unprecedented architectural complexity for a hybrid polyketide synthase and nonribosomal peptide synthetase. (nih.gov)
Our inter- and intra-institutional collaborations have enabled the development of a simple strategy for targeting antisense oligonucleotides and other therapeutics to macrophages, discovery of a new hemoglobin uptake mechanism in Leishmania donovani , elaboration of mycoketide biosynthesis catalyzed by polyketide synthases, and details of B cell responses to peptide epitopes and ascribing the role for a mycobacterial kinase. (rcb.res.in)
Biosynthesis of the 4-methyloxazoline-containing nonribosomal peptides, JBIR-34 and-35, in Streptomyces sp. (google.de)
Bacterial trans -acyltransferase polyketide synthases ( trans -AT PKSs) are among the most complex known enzymes from secondary metabolism and are responsible for the biosynthesis of highly diverse bioactive polyketides. (nature.com)
Helfrich, E. J. & Piel, J. Biosynthesis of polyketides by trans -AT polyketide synthases. (nature.com)
Biosynthesis of Vitroprocines by α-Oxoamine Synthase and Oxidoreductase Identified from Vibrio sp. (tmu.edu.tw)
In the flavonoid biosynthesis pathway, Chalcone synthase (CHS) is involved in the formation of the pigment and has been shown to be a rate-limiting enzyme for the synthesis of flavonoids. (scirp.org)

The phorboxazoles are mixed non-ribosomal peptide synthase/polyketide synthase biosynthetic products that embody polyketide domains joined via two serine-derived oxazole moieties. (fu-berlin.de)
It illustrates the utility of a synthetic design to form a mixed non-ribosomal peptide synthase/polyketide synthase biosynthetic product based upon biomimetic oxazole formation initiated by amide bond formation to join synthetic building blocks. (fu-berlin.de)

Ybt synthesis occurs by a mixed nonribosomal peptide synthetase (NRPS)/polyketide synthase (PKS) mechanism. (wikipedia.org)
Depending on the core gene, BGCs are grouped into the following major classes: non-ribosomal peptide synthetases (NRPSs), polyketide synthases (PKSs), hybrid non-ribosomal peptide synthetase-polyketide synthases (NRPS-PKSs), terpenes, and ribosomally synthesized and post-translationally modified peptides (RiPPs). (frontiersin.org)
AVM is biosynthetically derived from a hybrid cis -AT polyketide synthase-nonribosomal peptide synthetase (PKS-NRPS) pathway. (springernature.com)
Non-ribosomal peptide synthetases (NRPS) and polyketide synthases (PKS) found in bacteria, fungi and plants use two different types of thioesterases for the production of highly active biological compounds. (johnshopkins.edu)
The second enzyme involved in the synthesis of these secondary metabolites, the type II thioesterase (TEII), is a crucial repair enzyme for the regeneration of functional 4′-PP cofactors of holo-T domains of NRPS and PKS systems. (johnshopkins.edu)
During these studies, I became interested in a class of enzymes called non-ribosomal peptides synthetases (NRPS) which produce many highly complex peptide products that have important biological activity. (azolifesciences.com)
What are nonribosomal peptide synthetase (NRPS) enzymes and what role do they play within antibiotic drug discovery? (azolifesciences.com)
Nonribosomal peptide synthetases (NRPS) are large and highly complex enzymes that assemble standard and unusual amino acids substrates into highly complex peptide products, including many of the most important antibiotics in the clinic today (e.g. penicillin, vancomycin, and daptomycin). (azolifesciences.com)
NRPS are comprised of a series of modules organized into an assembly line architecture, with each module introducing a single amino acid precursor into a growing peptide chain. (azolifesciences.com)
In principle, the replacement of modules within the NRPS assembly line should enable a different amino acid building block to be introduced at a selected position, leading to user-defined peptide products. (azolifesciences.com)

Clusters encoding the putatively novel metabolic diversity comprise polyketide synthases (30), non-ribosomal peptide synthetases (12), and terpenes (1). (frontiersin.org)
5. The biosynthetic gene cluster for the antitumor drug bleomycin from Streptomyces verticillus ATCC15003 supporting functional interactions between nonribosomal peptide synthetases and a polyketide synthase. (nih.gov)

Their biosynthetic machinery, represented by type I polyketide synthases (PKSs), has an architecture in which successive modules catalyse two-carbon linear extensions and keto-group processing reactions on intermediates covalently tethered to carrier domains. (inra.fr)

isolated from the rhizospheric soil of Panax notoginseng: isolation, antimicrobial activity and biosynthetic potential for polyketides and non-ribosomal peptides. (nih.gov)

Nonribosomal Peptide Synthesis-Principles and Prospects. (research.com)
His work on Condensation domain is typically connected to Functional diversity and Assembly line as part of general Nonribosomal peptide study, connecting several disciplines of science. (research.com)

In plants, flavonoids are synthesized via the phenylpropanoid and polyketide pathway, which starts with the condensation of one molecule of CoA-ester of cinnamic acid or derivatives such as coumaric or ferulic acid, and three molecules of malonyl-CoA, yielding a naringen in chalcone as major product. (scirp.org)
Gluconeogenesis is a synthesis pathway of glucose from noncarbohydrate precursors. (easyomics.org)

Minimal deprotection then afforded phorboxazole A. This generally effective strategy was then dramatically abbreviated by employing a total synthesis approach wherein both of the natural product's oxazole moieties were installed simultaneously. (fu-berlin.de)
This synthetic strategy provides a total synthesis of phorboxazole A in 18% yield over nine steps from C3−C17 and C18−C30 synthetic fragments. (fu-berlin.de)
Bioinformatic analysis of the BGC combined with the development of a total synthesis route allowed for the elucidation of the molecule's absolute configuration. (bvsalud.org)

We have all the required skills to succeed in such an enterprise, involving discovery of new families of specialised metabolites, bacterial genomics, chemical synthesis and the associated molecular biology to manipulate genomes to enzymology and the reconstruction of biochemistry. (abdn.ac.uk)

The polyketide synthase (PKS) mega-enzyme assembly line uses a modular architecture to synthesize diverse and bioactive natural products that often constitute the core structures or complete chemical entities for many clinically approved therapeutic agents. (inra.fr)
Polyunsaturated fatty acid (PUFA) synthase is a multi-domain mega-enzyme that effectively synthesizes a series of PUFAs in marine microorganisms. (biomedcentral.com)

Sadaiwa Yorimoto, Akira Tsubouchi, Haruki Mizoguchi, Hideaki Oikawa, Yoshiaki Tsunekawa, Tomoya Ichino, Satoshi Maeda,* Hiroki Oguri* "Zn(OTf) 2 -mediated annulations of N -propargylated tetrahydrocarbolines: divergent synthesis of four distinct alkaloidal scaffolds" Chemical Science 2019 , 10 , 5686-5698. (u-tokyo.ac.jp)
2019) Diversification of polyketide structures via synthase engineering. (uic.edu)

2021) A Family of Nonribosomal Peptides Modulate Collective Behavior in Pseudovibrio Bacteria Isolated from Marine Sponges. (uic.edu)
Currently, oat is a global crop with production ranking seventh among cereals ( http://www.fao.org/faostat/en/ , accessed May 2021). (nature.com)

This is the first demonstration that tyramine can be produced from peptides containing tyrosine and therefore that free tyrosine is not the only precursor for tyramine production. (pdgfrsignals.com)
Both tyrDC and tyrP were significantly more strongly expressed during the early exponential growth phase in peptide medium (medium 2) than tyrosine medium (medium 1). (pdgfrsignals.com)

We demonstrate the applicative scope in several examples, including the characterization of new variants of bioactive natural products as well as structurally new polyketides from unusual bacterial sources. (nature.com)

STREPTOMYCES, using type II polyketide synthase involving condensation of acetyl CoA and 9 malonyl-CoA. (nih.gov)
Alchivemycin A (AVM, 1 ) is a potent antibacterial polycyclic polyketide with an unprecedented skeleton originally isolated from a plant-derived actinomycete Streptomyces sp. (springernature.com)
Here we used electron cryo-microscopy to determine sub-nanometre-resolution three-dimensional reconstructions of a full-length PKS module from the bacterium Streptomyces venezuelae that revealed an unexpectedly different architecture compared to the homologous dimeric mammalian fatty acid synthase. (inra.fr)

HN - 2024 FX - Neovascularization, Pathologic FX - Neovascularization, Physiologic MH - Angucyclines and Angucyclinones UI - D000095602 MN - D2.455.426.559.847.149.401 MN - D2.540.576.375 MN - D2.806.250.45 MN - D4.345.674.313 MN - D4.615.149.401 MS - Polycyclic aromatic polyketides with tetracyclic angular benz(a)anthracene skeletons. (nih.gov)

Exogenous nucleotide supplementation during times of rapid growth and stress is preferred because de novo synthesis is insufficient and energetically a costly process. (eurekaselect.com)

To enable de novo structural predictions for trans -AT PKS-derived polyketides, we developed the trans -AT PKS polyketide predictor (TransATor). (nature.com)
Polyketide natural products constitute a broad class of compounds with diverse structural features and biological activities. (inra.fr)
This study reveals for the first time, to our knowledge, the structural basis for both intramodule and intermodule substrate transfer in polyketide synthases, and establishes a new model for molecular dissection of these multifunctional enzyme systems. (inra.fr)

Haruki Mizoguchi, Hideaki Oikawa, Hiroki Oguri* "Biogenetically inspired synthesis and skeletal diversification of indole alkaloids" Nature Chemistry 2014 , 6 , 57-64. (u-tokyo.ac.jp)

We create synergy in protein engineering by fusing recombinant expression with chemical synthesis and enzymatic peptide ligation for potential applications in mechanistic biology and biotechnology. (rcb.res.in)

The unexpected module architecture revealed a striking evolutionary divergence of the polyketide synthase compared to its metazoan fatty acid synthase homolog, as well as remarkable conformational rearrangements dependent on its biochemical state during the full catalytic cycle. (inra.fr)

Modular polyketide synthases (PKS) produce a vast array of bioactive molecules that are the basis of many highly valued pharmaceuticals. (inra.fr)

Biswas T, Pawale VS, Choudhury D and Roy RP (2014) Sorting of LPXTG peptide substrate by archetypal Sortase A. Biochemistry 53: 2515-24. (rcb.res.in)
The ACP domain is differentially and precisely positioned after polyketide chain substrate loading on the active site of the ketosynthase, after extension to the beta-keto intermediate, and after beta-hydroxy product generation. (inra.fr)
Captisol Since plasma lactate is not a primary substrate for www.selleckchem.com/products/H-89-dihydrochloride.html glycogen synthesis in the fed state [36], it is possible that a higher percentage of glucose was taken up by the muscle and stored as glycogen after Cereal rather than converted to lactate. (pdgfrsignals.com)

The first 3D structures of a full polyketide synthase module in different functional states were obtained recently by electron cryo-microscopy. (inra.fr)

2020) Heterologous Production of Lasso Peptide Capistruin in a Burkholderia Host. (uic.edu)
Yeh CJ, Zulueta MML, Li YK, Hung SC* , 2020, "Synthesis of hyaluronic acid oligosaccharides with a GlcNAc-GlcA repeating pattern and their binding affinity with CD44. (sinica.edu.tw)

Löhr NA, Rakhmanov M, Wurlitzer JM, Lackner G, Gressler M, Hoffmeister D (2023) Basidiomycete non-reducing polyketide synthases function independently of SAT domains. (leibniz-hki.de)
Yang YL, Zhou M, Yang L, Gressler M, Rassbach J, Wurlitzer JM, Zeng Y, Gao K, Hoffmeister D (2023) A mushroom P450-monooxygenase enables regio- and stereoselective biocatalytic synthesis of epoxycyclohexenones. (leibniz-hki.de)

Total syntheses of phorboxazole A and analogues have been developed that rely upon the convergent coupling of three fragments via biomimetically inspired de novo oxazole formation. (fu-berlin.de)

His study in Peptide is interdisciplinary in nature, drawing from both Amino acid, Biophysics and Combinatorial chemistry. (research.com)

His biological study spans a wide range of topics, including Ribosomal RNA, Stereochemistry, Glycopeptide, Halogenation and Peptide synthesis. (research.com)

However, optimal combinations of a heterologous protein, suitable signal peptide and secretion host can currently not be predicted, resulting in large strain libraries that need to be tested. (authorea.com)

His Peptide research integrates issues from Molecular recognition and Enzyme. (research.com)

Current work in our laboratory is focused toward understanding the mechanistic imperatives of sortase-catalyzed protein/peptide ligation and exploring innovative strategies to expand the synthetic ambit of this multifaceted enzyme. (rcb.res.in)

As glycogen accretion occurs, glycogen synthase becomes phosphorylated, reducing glycogen synthase activity. (pdgfrsignals.com)

Kumaran S, Datta D, Roy RP (2000) An enigmatic peptide ligation reaction. (rcb.res.in)
The Hantzsch pyrrole synthesis is the reaction of β-ketoesters ( 1 ) with ammonia (or primary amines) and α-haloketones ( 2 ) to give substituted pyrroles ( 3 ). (cloudfront.net)
In contrast, the carrier from the preceding module uses a separate entrance outside the reaction chamber to deliver the upstream polyketide intermediate for subsequent extension and modification. (inra.fr)

Macrocyclization is an important process in bioactive natural product synthesis. (springernature.com)

We are interested in developing chemical biology tools for protein semi-synthesis, labeling and bioconjugation. (rcb.res.in)
The two FabA-like DH domains in the E. huxleyi PUFA synthase function as 3-hydroxyacyl-acyl carrier protein dehydratase in E. coli . (biomedcentral.com)

Anatomy2

Organisms6

Chemicals and Drugs24

Analytical, Diagnostic and Therapeutic Techniques and Equipment4

Phenomena and Processes20

Technology, Industry, Agriculture1

Information Science3

Health Care1

Biosynthesis11

Nonribosomal peptide4

Genes4

Enzymes1

Synthetase1

Acid synthase1

Hybrids1

Putative1

Proteins2

Metabolism2

Mycobacterium1

Antibiotics2

Symbionts1

Amino2

Modular1

Secondary1

Bacteria1

Linear1

Natural1

Evaluation1

DATA1

Activity1

Biosynthesis18

Mixed non-ribosomal2

NRPS10

Synthetases2

PKSs1

Biosynthetic1

Nonribosomal Peptide2

Pathway2

Total synthesis3

Metabolites1

Mega-enzyme2

20192

20212

Tyrosine2

Bacterial1

STREPTOMYCES3

Aromatic1

Novo Synthesis1

Structural3

Diversification1

Chemical synthesis1

Catalytic1

Bioactive molecules1

Substrate3

Structures1

20202

20232

Analogues1

Amino1

Biological1

Heterologous1

Molecular1

Enzyme1

Occurs1

Reaction3

Natural product1

Protein2