• Kalmar GB, Kay RJ, LaChance AC, Cornell RB: Primary structure and expression of a human CTP:phosphocholine cytidylyltransferase. (drugbank.com)
  • Johnson JE, Cornell RB: Membrane-binding amphipathic alpha-helical peptide derived from CTP:phosphocholine cytidylyltransferase. (drugbank.com)
  • Dunne SJ, Cornell RB, Johnson JE, Glover NR, Tracey AS: Structure of the membrane binding domain of CTP:phosphocholine cytidylyltransferase. (drugbank.com)
  • Xie M, Smith JL, Ding Z, Zhang D, Cornell RB: Membrane binding modulates the quaternary structure of CTP:phosphocholine cytidylyltransferase. (drugbank.com)
  • The Kennedy pathway begins with the direct phosphorylation of free choline into phosphocholine followed by conversion into CDP-choline and subsequently phosphatidylcholine. (smpdb.ca)
  • The first reaction of the Kennedy pathway involves the cytosol-localized enzyme choline/ethanolamine kinase catalyzing the conversion of choline into phosphocholine. (smpdb.ca)
  • Second, choline-phosphate cytidylyltransferase, localized to the endoplasmic reticulum membrane, catalyzes the conversion of phosphocholine to CDP-choline. (smpdb.ca)
  • At early stages of Alzheimer's disease, phosphomonoesters (PMEs) including phosphocholine (P-choline) are present at elevated levels. (uky.edu)
  • The lesion-induced elevation in P-choline could result from increased P-choline synthesis via choline kinase, decreased activity of CTP:phosphocholine cytidylyltransferase, or breakdown of phosphatidylcholine (PC). (uky.edu)
  • Metabolic labeling experiments in whole cells showed that NMDA receptor overactivation does not modify the activity of phosphocholine or phosphoethanolamine cytidylyltransferases but strongly inhibits choline-ethanolamine phosphotransferase activity. (jneurosci.org)
  • 0.001) of breast cancer cells compared with HMECs, indicating a higher metabolic flux from membrane phosphatidylcholine to choline and phosphocholine in breast cancer cells. (aacrjournals.org)
  • The magnetic resonance spectroscopy data indicated that elevated phosphocholine in breast cancer cells was primarily attributable to increased choline kinase activity and increased catabolism mediated by increased phospholipase C activity. (aacrjournals.org)
  • Over the past decade, one common feature consistently revealed by magnetic resonance (MR) spectroscopic studies is the elevation of phosphocholine (PC) and total choline-containing metabolites [tCho, glycerophosphocholine (GPC)+PC+free choline (Cho)] in cancer cells and solid tumors. (aacrjournals.org)
  • Both phospholipids were maintained at or above control levels at each of the postlesion time points, arguing against membrane breakdown or decreased PC synthesis contributing to the elevation in P-choline levels. (uky.edu)
  • We now investigated the effect of NMDA receptor overactivation on synthesis and degradation of major membrane phospholipids in the early stages of the excitotoxic process. (jneurosci.org)
  • These results show that membrane damage by NMDA is preceded by inhibition of phospholipid synthesis and not by phospholipid degradation in the early stages of the excitotoxic process, and that NMDA receptor overactivation decreases phosphatidylcholine and phosphatidylethanolamine synthesis by inhibiting choline-ethanolaminophosphotransferase activity. (jneurosci.org)
  • Enzymatic hydrolysis of membrane phospholipids by phospholipases produces these lipid second messengers, as well as soluble second messengers such as inositol 1,4,5-trisphosphate, and also other molecules such as choline (Cho) that are released to the extracellular medium. (jneurosci.org)
  • In this study, differences in choline membrane metabolism were characterized in breast cancer cells and normal human mammary epithelial cells (HMECs) labeled with [1,2- 13 C]choline, using 1 H and 13 C magnetic resonance spectroscopy. (aacrjournals.org)
  • Metabolic fluxes between membrane and water-soluble pool of choline-containing metabolites were assessed by exposing cells to [1,2- 13 C]choline for long and short periods of time to distinguish between catabolic and anabolic pathways in choline metabolism. (aacrjournals.org)
  • Choline-phosphate cytidylyltransferase (EC 2.7.7.15) is an enzyme that catalyzes the chemical reaction CTP + choline phosphate ⇌ {\displaystyle \rightleftharpoons } diphosphate + CDP-choline where the two substrates of this enzyme are CTP and choline phosphate, and the two products are diphosphate and CDP-choline. (wikipedia.org)
  • Choline kinase and phospholipase C were significantly overexpressed, and lysophospholipase 1, phospholipase A2, and phospholipase D were significantly underexpressed, in breast cancer cells compared with HMECs. (aacrjournals.org)
  • These observations were consistent with the overexpression of choline kinase and phospholipase C detected in the microarray analyses. (aacrjournals.org)
  • METHODS: In maternal, umbilical cord plasma and placentas from obese women (11 female/5 male infants) and normal weight women (9 female/7 male infants), all PC and PE species containing DHA and ARA were analyzed by LC-MS/MS. Placental protein expression of enzymes involved in phospholipid synthesis, were determined by immunoblotting. (bvsalud.org)
  • The systematic name of this enzyme class is CTP:choline-phosphate cytidylyltransferase. (wikipedia.org)
  • A parallel Kennedy pathway forms phosphatidylethanolamine from ethanolamine - the only difference being a different enzyme, ethanolamine-phosphate cytidylyltransferase, catalyzing the second step. (smpdb.ca)
  • An enzyme that catalyzes the transfer of UMP from UDPglucose to galactose 1-phosphate, forming UDPgalactose and glucose 1-phosphate. (nih.gov)
  • Exposure of cortical neurons to neurotoxic concentrations of NMDA increased extracellular choline and activated hydrolysis of phosphatidylcholine and phosphatidylinositol by phospholipase A 2 but did not induce significant degradation of phosphatidylcholine, phosphatidylinositol, phosphatidylethanolamine, or phosphatidylserine. (jneurosci.org)
  • The aberrant choline phospholipid metabolism of breast cancer cells and its strong correlation with malignant progression has been observed in several studies. (aacrjournals.org)
  • Other alterations included a widespread elevation in inositol phosphate 2 days postlesion, but not at later time points. (uky.edu)
  • Last, choline/ethanolaminephosphotransferase catalyzes phosphatidylcholine biosynthesis from CDP-choline. (smpdb.ca)
  • It is the major synthesis route in animals. (smpdb.ca)
  • We demonstrated for the first time, that female fetuses of obese mothers decrease placental ether and plasmalogen PE containing DHA and arachidonic acid (ARA, omega 6), and show a high fetal-placental adaptability and placental reserve capacity that can maintain the PC-LCPUFA synthesis and the transfer of these crucial species to the fetus to preserve brain development. (bvsalud.org)
  • RESULTS Choline phosphate cytidylyltransferase-α (CCTα, also known as phosphate cytidylyltransferase 1 choline alpha [PCYT1A], a phospholipid synthesis enzyme regulated by RAS) is the second antigen recognized by 8F1. (usuhs.edu)
  • Last, choline/ethanolaminephosphotransferase catalyzes phosphatidylcholine biosynthesis from CDP-choline. (smpdb.ca)
  • This gene belongs to the cytidylyltransferase family and is involved in the regulation of phosphatidylcholine biosynthesis. (nih.gov)
  • Synthesis of phosphatidylcholine, the major phospholipid of animal cell membranes, requires the key enzyme cytidylyltransferase (CCTalpha). (nih.gov)
  • An enzyme that catalyzes the transfer of cytidylate (CMP) to choline phosphate to form CDPcholine. (bvsalud.org)
  • A parallel Kennedy pathway forms phosphatidylethanolamine from ethanolamine - the only difference being a different enzyme, ethanolamine-phosphate cytidylyltransferase, catalyzing the second step. (smpdb.ca)
  • It is the rate-limiting enzyme in the choline pathway for the biosynthesis of phosphatidylcholine. (bvsalud.org)
  • This study was to investigate the effects and related mechanisms of Glu on the lipid synthesis of pulmonary surfactant (PS) in isolated rat lung tissues. (bvsalud.org)
  • In conclusion, NMDAR-mediated Glu toxicity leading to impaired PS synthesis may be a potential pathogenesis of lung injury. (bvsalud.org)
  • Stearoyl-CoA desaturase 1 deficiency up-regulates beta-oxidation and decreases de novo ceramide synthesis in skeletal muscle. (edu.pl)
  • It is the major synthesis route in animals. (smpdb.ca)