CatalysisEnzymesKineticsBiocatalysisModels, MolecularSubstrate SpecificityCatalytic DomainBinding SitesModels, ChemicalMolecular Sequence DataCrystallography, X-RayQuantum TheoryProtein ConformationAmino Acid SequenceEscherichia coliMutagenesis, Site-DirectedHydrogen-Ion ConcentrationStereoisomerismThermodynamicsMolecular StructureMutationStructure-Activity RelationshipEnzyme StabilityDecarboxylationHydrogenHydrogen BondingBase SequenceProtein BindingHydrolysisOxidation-ReductionRecombinant ProteinsHistidineMolecular ConformationTetrahydrofolate DehydrogenaseStatic ElectricityMagnetic Resonance SpectroscopyProtein Structure, TertiaryBacterial ProteinsTemperatureProtein BiosynthesisSolventsChromatography, High Pressure LiquidSequence Homology, Amino AcidPseudomonasProteinsSpectrophotometryCloning, MolecularOxidoreductasesAmino AcidsAmino Acid SubstitutionNADPCysteineProtonsProtein Structure, SecondaryMolecular Dynamics SimulationAdenosine TriphosphateEnzyme ActivationNuclear Magnetic Resonance, BiomolecularWaterComputer SimulationProtein Synthesis InhibitorsMolecular WeightCyclizationRNA, MessengerOxygenDNACells, CulturedCycloheximideDNA ReplicationCell LineLeucineChemistry, OrganicTritiumLiverTranscription, GeneticCarbon IsotopesChemistry Techniques, SyntheticTime FactorsRNACell-Free SystemDactinomycinRNA, CatalyticElectrophoresis, Polyacrylamide GelPalladiumAlkenesPuromycinThymidineArgininePeptide BiosynthesisUridineMagnesiumNucleic Acid ConformationSequence AlignmentSaccharomyces cerevisiaeEnzyme InhibitorsPlasmidsIndicators and ReagentsPhenylalanineCell DivisionChloramphenicol