Adenocarcinoma ex-goblet cell carcinoid (AdexGCC) was considered a neuroendocrine adenocarcinoma, despite majority of tumor cells being negative for conventional neuroendocrine markers such as chromogranin and synaptophysin. (gastrores.org)
INSM1 exhibits low expression in AdexGCCs and is expressed by a lower proportion of tumor cells compared to chromogranin and synaptophysin. (gastrores.org)
Conventional neuroendocrine markers, such as chromogranin and synaptophysin, however, are often negative or focally positive in these tumors [ 2 , 3 ]. (gastrores.org)
Hristov et al previously demonstrated that chromogranin and synaptophysin were only expressed in 37% and 30% of AdexGCCs, respectively [ 2 ]. (gastrores.org)
In contrast, conventional carcinoids are often diffusely positive for chromogranin and synaptophysin, with abundant electron-dense granules on electron microscopy [ 4 ]. (gastrores.org)
The neoplastic cells routinely express pan-cytokeratin and neuroendocrine markers chromogranin and synaptophysin (focal to diffuse staining). (pbpath.org)
In immunohistochemical studies, the spindle cells were positive for glial fibrillary acidic protein (GFAP) (Fig. 2c ) and S-100 protein (Fig. 3a ). (biomedcentral.com)
Immunohistochemical examinations showed that the tumor cells were synaptophysin- and NeuN-positive but GFAP-negative. (biomedcentral.com)
Immunohistochemical findings showed positive staining for cytokeratin (CK) 7 but no reactivity with antibodies against TTF1, CK5/6, p63 and napsin. (biomedcentral.com)
synaptophysin or chromogranin positive by immunohistochemical staining is insufficient to diagnose SCC). (trialstoday.org)
S1006
only the basal cells are positive with CK5/6, p63, S100 protein. (wikipedia.org)
MCC express both neuroendocrine (neuron-specific enolase, synaptophysin, chromogranin) and cytokeratin markers (cytokeratin 20, as a paranuclear dot, CAM 5.2) and is negative for S100 and the common leukocyte antigen ( 2 ). (ispub.com)
The clear and round cells were positive for S100, synaptophysin and Olig2 (Figure 1k ). (upmc.edu)
The tumors were screened for neural differentiation with an antibody panel consisting of neuron-specific enolase, S100 protein, Leu-7, chromogranin, synaptophysin, and neurofilament. (nih.gov)
Three cases of ES were positive for S100 protein. (nih.gov)
this feature is seen in the majority of ependymomas ( Image C ). A less common but more specific feature is the presence of tubules and canals of tumor cells with an ependymal appearance, often with apical cilia or microvilli (true ependymal rosettes, Image B ). Ependymomas are generally S100 positive and GFAP positive, and the latter is accentuated in the cell processes composing the perivascular pseudorosettes. (cap.org)
CD564
Immunohistochemically, the tumor was positive for chromogranin A, CD56, and synaptophysin, and a diagnosis of paraganglioma of the urinary bladder was confirmed. (biomedcentral.com)
Recently, insulinoma-associated protein 1 (INSM1) has been identified as a novel neuroendocrine marker that is more sensitive than chromogranin, synaptophysin, and CD56 in pulmonary neuroendocrine tumors. (gastrores.org)
We studied this marker in conjunction with chromogranin, synaptophysin, and CD56 in 36 appendiceal AdexGCCs (21 primaries, 15 metastatic). (gastrores.org)
Primary and metastatic AdexGCCs showed no difference in INSM1, chromogranin, synaptophysin, or CD56 staining. (gastrores.org)
Glial fibrill3
Each of the cell lines also reacted with antibodies against neural cell adhesion molecules, but none of them were positive for antibodies against glial fibrillary acidic protein, keratin, microtubule-associated protein tau and microtubule-associated protein 2, human lymphocyte antigen-DR, epidermal growth factor receptor, and T-cell antigen. (duke.edu)
In summary, the detection of neurofilament proteins and synaptophysin and the absence of glial fibrillary acidic protein provide strong evidence for a neuronal phenotype of D384 Med, D425 Med, and D458 Med. (duke.edu)
Tissue blocks were cut into 4-μm slides, deparaffinized in xylene, rehydrated with graded alcohols, and immunostained with the following antibodies: cytokeratin (CK, AE1/AE3), glial fibrillary acidic protein (GFAP, GA-5), synaptophysin (SP11), NeuN (A60) and Ki67 (MIB-1) (MaiXin, China). (biomedcentral.com)
Neurofilament2
Immunohistochemistry was performed in both tissue and 3D sections for markers including synaptophysin, vimentin, neurofilament and MIB-1. (frontiersin.org)
Neurofilament and synaptophysin were negative in either the spindle cells or the large cells (Fig. 3b ), and KI67 was not detectable (Fig 3c ). (biomedcentral.com)
Neuron-specifi1
The PNET and one case of ES were positive for neuron-specific enolase. (nih.gov)
Olig21
These tumors also display EMA staining at the surfaces of ependymal rosettes/canals, as well as in a dot-like or ring-like pattern adjacent to tumor nuclei ( Image D ). Neurofilaments, synaptophysin, OLIG2, SOX10, and NeuN are usually negative. (cap.org)
GFAP2
Lentivirus-mediated overexpression of GATA3 significantly increased the number of GFAP/SOX2 double positive astrocytes and expression of pro-neural factor ASCL1, but failed to induce neurogenesis, suggesting that GATA3 is required for enhancing the neurogenic potential of primary human astrocytes and is not sufficient to induce neurogenesis alone. (frontiersin.org)
GATA3 increases the GFAP/SOX2-positive cells and ASCL1 after injury in 3D. (frontiersin.org)
Synaptophysin is a protein that is found in neurons. (flebo.in)
Neuroendocrine tumors3
Synaptophysin is a glycoprotein that is a component of the membrane of neuroendocrine secretory granules and is identified by monoclonal antibodies in a number of neuroendocrine tumors. (flebo.in)
The Synaptophysin Test is run to detect the neuroendocrine tumors in the cells. (flebo.in)
There are only two interpretations of the synaptophysin test: either the neuroendocrine tumors are present in the body or they are absent. (flebo.in)
The luminal epithelial cells were positive for cytokeratin AE1/AE3, C-kit, and CEA, while the abluminal myoepithelial cells were positive for P63 and SMA (Figure 3 ). (hindawi.com)
6 ] It originates from specialized cytokeratin-positive and nestin-positive ependymal cells derived from the subcommissural organ. (surgicalneurologyint.com)
Immunohistochemistry2
Synaptophysin Test is the most reliable immunohistochemistry marker for identifying the abnormal tumors causing cancer. (flebo.in)
When the neuroendocrine tumor is present, the test is said to be positive, else negative. (flebo.in)
The histological examination from the FNA sample revealed a scarce specimen for evaluation, with columnar epithelial cells with mild atypia, positive for CK7 and 19, negative for p53, chromogranin A and synaptophysin. (spg.pt)
The neoplastic cells were positive for pan-CK (strong and diffuse), synaptophysin (strong and diffuse) [Figure 2], chromogranin (strong but focal) and inhibin (strong and diffuse) while negative for CK7, RCC, PAX-8, S-100, Melan-A (MART-1), and beta catenin. (pbpath.org)
Cytologic evaluation shows large discohesive tumor cells that are positive for CK AE1 / 3, CK7, with variable positivity for TTF-1 and focal positivity for Napsin-A. The tumor cells are negative for p40 and synaptophysin. (lungevity.org)
Fine needle asp2
A fine needle aspiration was positive for MCC. (ispub.com)
Endoscopic ultrasonography-guided fine-needle aspiration revealed CD34-positive spindle-shaped cells in the fibrous tissue, suggestive of a mesenchymal neoplasm. (biomedcentral.com)
As synaptophysin is a maker of these neuroendocrine tissues, it forms a reliable source to test the formation of tumors. (flebo.in)
Cells8
the abluminal myoepithelial cells of the tumor are positive for SMA and P63. (hindawi.com)
Normal background structures, such as vascular endothelial cells, retain H3K27me3 immunoexpression and serve as a useful internal positive control. (cap.org)
D-Detection of synaptophysin is evident with mild to moderate cytoplasmic staining (arrows) of a portion of the tumor cells. (avma.org)
Anti-human lymphocyte antigen-A,B and anti-beta 2-microglobulin antibodies reacted with xenografts of D384 Med and D425 Med and were weakly positive for a small population of D384 Med cultured cells. (duke.edu)
Loss of both PEDF and PEDF-R caused retinas to have thinner outer nuclear layers with TUNNEL positive photoreceptor cells, as well as decreased levels of rhodopsin, opsin, PKCα, and synaptophysin compared to controls. (nih.gov)
The tumor cells were positive for AE1/AE3, CD10, WT-1, and the hormone calretinin. (athademu.com)
The tumor cells were also positive for AE1/AE3, CD10 and mesothelioma attorney in elsmere WT-1. (kastellet-vel.no)
Figure1
Figure 3: A focal positive reaction was observed for the pancytokeratin stain. (ispub.com)
CD1171
CD117 can be positive in either population. (wikipedia.org)
Results1
New therapeutic techniques such as photodynamic therapy, immunotherapy, and intravitary chemotherapy are showing positive results. (kastellet-vel.no)
Cell1
Following medical optimization, she underwent thoracoscopic thymectomy with en bloc thymic small-cell carcinoma resection. (bvsalud.org)
Sensitive1
The edrophonium chloride (Tensilon) test is sensitive but has a significant false positive rate. (jomi.com)
Case1
In this report, we describe the fourth case of an RGNT being isolated to the lateral ventricles and the first where it stained positive for EMA and D2-40. (surgicalneurologyint.com)
Multiple1
The primary tumor was 4 cm in size, with multiple positive margins and lingual nerve involvement. (hindawi.com)