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  • gene
  • Using a Δ cbrA null mutant, which leads to increased levels of CtrA in S. meliloti , and the β-glucuronidase (GUS) reporter gene, I can monitor the expression levels of target genes that are potentially regulated by CbrA and CtrA. (umb.edu)
  • Results
  • These results are significant because no one has looked at genes regulated by CbrA or CtrA in S. meliloti , which is more applicable to host-microbe interactions than C. crescentus , especially since Agrobacterium tumefaciens and Brucella abortus both have a CbrA homologue. (umb.edu)
  • cell cycle
  • Using genes known to be regulated by CtrA in C. crescentus or predicted to be regulated by CtrA in S. meliloti , I aim to show how certain cell cycle genes are regulated in S. meliloti . (umb.edu)
  • symbiosis
  • Our data showed that (i) inoculation with rhizobia could improve the ability of the associated host-plant to resist a pathogen attack and (ii) A. euteiche infection adversely impacts the S. meliloti -alfalfa symbiosis. (wsu.edu)
  • Surface sterilization of the eggs resulted in aposymbiotic insects of slower growth, smaller size and abnormal body coloration, indicating vertical symbiont transmission via egg surface contamination and presumable beneficial nature of the symbiosis. (jove.com)
  • The proposed research addresses critical problems concerning how host antimicrobial peptides modulate S. meliloti's cell cycle and physiology during symbiosis, how the master regulator CtrA acts to control S. meliloti cell cycle progression and terminal differentiation during symbiosis, and how the RNase YbeY exerts its multiple biological roles. (grantome.com)
  • We will gain insights into how regulation of the S. meliloti cell cycle has been adapted for symbiosis by elucidating the mechanism by which NCR247 alters cell cycle regulation and blocks cell division, defining the direct transcriptional targets of S. meliloti cel cycle regulator CtrA, and analyzing the role of CtrA in regulating physiological processes relevant to symbiosis. (grantome.com)
  • genes and signals in the symbiosis between Sinorhizobium meliloti and its host, Medicago sativa . (fullerton.edu)
  • Remarkably, not only are the ExoS/ChvI genes essential for the S. meliloti -plant symbiosis, but the orthologs of the ExoS/ChvI genes in Brucella are essential for causing disease in mammals. (fullerton.edu)
  • Rhizobia
  • There have been many genetic analyses of rhizobia, notably of Sinorhizobium meliloti (the symbiont of alfalfa), Bradyrhizobium japonicum (soybean), and Rhizobium leguminosarum, which has biovars that nodulate peas and broad beans (biovar viciae), clovers (biovar trifolii), or kidney beans (biovar phaseoli). (paperity.org)
  • In particular, the effect of extracellular acidity on rhizobia has been taken as a model example for analysis because of the economic impact and worldwide distribution of these symbionts in agricultural countries. (omictools.com)
  • inoculation
  • However the sterile growth-chamber experiments could be used to study the effect of S. meliloti inoculation on the development of Aphanomyces root rot. (wsu.edu)
  • Several of the changes included defense- and redox-related proteins, and visualization of reactive oxygen species indicated that their induction in root hairs following Sinorhizobium inoculation was inhibited by nitrate. (mdpi.com)
  • Species
  • However, exceptional cases have been reported from two subsocial species representing the closely related families Cydnidae and Parastrachiidae, wherein females remain nearby eggs for maternal care after oviposition, and provide their offspring with symbiont-containing secretions at later stages, either just before or after hatching. (jove.com)
  • Sinorhizobium and Rhizobium species) and plant and animal pathogens (eg. (prolekare.cz)
  • Although those assessments are largely valid in the sense that the organismic interactions in rhizospheres are poorly defined outside of a few species of microorganisms important as pathogens or symbionts, what is missed is the wealth of molecular data and tools developed for work in laboratory rhizospheres with microbiologically controlled conditions. (plantphysiol.org)
  • Agrobacterium
  • A "landing pad" (LP) sequence consisting of a spectinomycin- resistance gene, aadA ( SpR ), and the E. coli ß-glucuronidase gene ( uidA ) flanked by attP sites has been integrated into the chromosomes of Sinorhizobium meliloti, Ochrobactrum anthropi, and Agrobacterium tumefaciens in an intergenic region, the ampC locus, and the tetA locus, respectively. (jove.com)
  • Genomic
  • It was demonstrated that, in the examined geographically distinct populations of S. meliloti from the Northern Caucasus (NCG) and the Aral Sea region (PAG), the strains containing genomic islands were observed with similar frequency (0.55 and 0.57, respectively). (deepdyve.com)
  • progeny
  • But, if the progeny encounter an environment that differs from that of their parent, they may be disadvantaged if the inherited symbionts prove suboptimal. (jove.com)
  • By releasing progeny with maternally derived symbionts, that are also capable of subsequent horizontal symbiont acquisition, coral colonies may acquire physiologically advantageous novel symbionts that are then perpetuated via vertical transmission to subsequent generations. (jove.com)
  • flavonoids
  • Both interactions require chemical communication for the establishment of the association that involves the release of diffusible signals from the plant, strigolactones and flavonoids, and the production of diffusible signals from the symbionts ( Oldroyd, 2013 ). (plantcell.org)
  • enzyme
  • NAD(P)-malic enzyme (DME) of S. meliloti is required for efficient metabolism of succinate via the TCA cycle. (beds.ac.uk)
  • it represents the first enzyme in the degradative arm of the PHB cycle in S. meliloti and is the only enzyme in this half of the PHB cycle that remains uncharacterized. (biomedcentral.com)
  • infection
  • The comparison of these assay showed that the greenhouse experiments are more reliable for detection of the effect of A. euteiche infection on S. meliloti -alfalfa association. (wsu.edu)
  • genus
  • Using denaturing-gradient gel electrophoresis and real-time PCR, we identified the dinoflagellate symbionts (genus Symbiodinium) hosted by the Red Sea coral Stylophora pistillata throughout its ontogenesis and over depth. (jove.com)
  • However, we found that cydnid stinkbugs of the genus Adomerus, which are associated with gammaproteobacterial gut symbionts and exhibit elaborate maternal care over their offspring, smear symbiont-containing secretions onto eggs upon oviposition as many non-social stinkbugs do. (jove.com)
  • However
  • In horizontal acquisition, however, a potentially advantageous symbiont will not be transmitted to subsequent generations. (jove.com)
  • host
  • Conversely, while in horizontal symbiont acquisition host survival hinges on an unpredictable symbiont source, an individual host may acquire genetically diverse symbionts well suited to any given environment. (jove.com)
  • We describe a method for visualization of a bacterial symbiont within or on a nematode host, taking advantage of the optical transparency of nematodes when viewed by microscopy. (jove.com)
  • These observations suggested that sociality of the host stinkbugs may be correlated with their symbiont transmission strategies. (jove.com)
  • These results suggest that not only sociality but also other ecological and evolutionary aspects of the host stinkbugs, including the host-symbiont co-evolutionary history, may have substantially affected their symbiont transmission strategies. (jove.com)
  • Several methods have been developed for long-term growth and observation of this host/symbiont pair. (jove.com)
  • plant
  • The plant and its bacterial symbiont were used in a landmark University of Wisconsin-Madison study to detail the proteins involved in the process of nitrogen fixation, where plant nutrients are drawn from the atmosphere. (wisc.edu)
  • Background
  • Background Rhizobium leguminosarum is an α-proteobacterial N2-fixing symbiont of legumes that has been the subject of more than a thousand publications. (paperity.org)