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  • protein
  • Mitogen-activated S6 kinase is stimulated via protein kinase C-dependent and independent pathways in Swiss 3T3 cells. (semanticscholar.org)
  • Soluble extracts prepared from quiescent Swiss mouse 3T3 cells that had been briefly exposed to various mitogens exhibited a 2- to 3-fold elevation in phosphorylating activities toward ribosomal protein S6 and a synthetic peptide, Arg-Arg-Leu-Ser-Ser-Leu-Arg-Ala (RRLSSLRA), patterned after a phosphorylation site sequence from S6. (semanticscholar.org)
  • In a double immunofluorescence labeling experiment, presented above, an adherent culture of Swiss mouse embryo cells was treated with a cocktail of mouse anti-histones (pan) and rabbit anti-PMP 70 (peroxisomal membrane protein) primary antibodies, followed by goat anti-mouse and anti-rabbit secondary antibodies conjugated to Alexa Fluor 568 and Alexa Fluor 488, respectively, to target the nuclear histone proteins and peroxisomes. (fsu.edu)
  • Each Rho protein affects numerous proteins downstream, all of which having roles in various cell processes. (wikipedia.org)
  • TCTP is a multifunctional and highly conserved protein that existed ubiquitously in different eukaryote species and distributed widely in various tissues and cell types. (wikipedia.org)
  • TCTP was originally described as a growth related protein of tumor cells. (wikipedia.org)
  • Research in 1997 shown that TCTP is not a tumor- or tissue-specific protein, but is expressed ubiquitously from plants to mammals TCTP is a 20-25 kDa protein abundantly and ubiquitously expressed in the cell. (wikipedia.org)
  • The protein is transcribed in more that 500 different tissues and cell types, hTCTP gene is one of the top 10 most ubiquitously expressed genes in humans by examining 1753 libraries from kinds of tissues, but differed considerably in their quantity and ratio of expression, the expression is lower in kidney and renal cells. (wikipedia.org)
  • TCTP has properties of a tubulin binding protein that associates with microtubules in a cell cycle-dependent manner. (wikipedia.org)
  • Downregulation of the protein levels by siRNA in HTR-8/SVneo (Homo sapiens placenta cells) was associated with a reduced cellular calcium-uptake activity and buffering capacity. (wikipedia.org)
  • Translationally Controlled Tumor Protein (TCTP/tpt1) is a regulator of the cancer stem cell compartment, the tumor reversion, tumor progression and certain forms of inflammatory diseases. (wikipedia.org)
  • The activity of this protein has been implicated in controlling cell growth and differentiation. (wikipedia.org)
  • Immunofluorescence and antibody techniques were used to localise the mutant V12rac1 protein after being microinjected into the cytoplasm of confluent serum-starved Swiss 3T3 cells. (wikipedia.org)
  • Another group discovered a novel cDNA species encoding a protein with similar structure to COX-1 while studying phorbol-ester-induced genes in Swiss 3T3 cells. (wikipedia.org)
  • They showed that opposing gradients of bone morphogenetic protein (BMP) and Nodal, two transforming growth factor family members that act as morphogens, are sufficient to induce molecular and cellular mechanisms required to organize, in vivo or in vitro, uncommitted cells of the zebrafish blastula animal pole into a well-developed embryo. (wikipedia.org)
  • Proceedings of the Royal Society Series B: Biological Sciences 147: 268-73 Staeheli P, Haller O, Boll W, Lindenmann J, Weissmann C. (1986) Mx protein: constitutive expression in 3T3 cells transformed with cloned Mx cDNA confers selective resistance to influenza virus. (wikipedia.org)
  • pathway
  • The response to mating pheromones as described in Hartwell's experiments is unsurprising considering the antagonistic biochemical interactions between the mating pathway and the G1 cyclins that promote cell cycle progression. (wikipedia.org)
  • quiescent
  • Moreover, they can make this transition even in the absence of noticeable injuries and are capable of replenishing entire gastric units, in essence serving as quiescent "reserve" stem cells. (wikipedia.org)
  • tissue
  • The cells were found to be capable of regenerating epidermal tissue in a relatively short period of time, although the production of the anchoring networks needed to connect this tissue with underlying tissue layers was significantly less rapid. (fsu.edu)
  • This cell type also produces glycosaminoglycans, fibers, and glycoproteins that together comprise the extracellular matrix of connective tissue. (fsu.edu)
  • Todaro and Green originally obtained their 3T3 cells from Swiss albino mouse embryo tissue. (wikipedia.org)
  • Thus the initial goal in isolating and culturing cells from this tissue was not to generate a new model system for epithelial cell biology. (wikipedia.org)
  • In their report, the authors speculated that the "histotypic expression" by which MDCK cells formed structures reminiscent of their tissue of origin might be fruitfully applied to the study of other tissues. (wikipedia.org)
  • Through the 1970s, the MDCK cell line found new use as a model for mammalian epithelial tissue. (wikipedia.org)
  • This work solved an outstanding mystery of MDCK culture, as the tissue from which these cells were derived is tubular, yet they had previously only developed into spherical acini in 3D culture. (wikipedia.org)
  • After injury, mature terminally differentiated kidney cells dedifferentiate into more primordial versions of themselves and then differentiate into the cell types needing replacement in the damaged tissue Macrophages can self-renew by local proliferation of mature differentiated cells. (wikipedia.org)
  • In newts, muscle tissue is regenerated from specialized muscle cells that dedifferentiate and forget the type of cell they had been. (wikipedia.org)
  • 1970s
  • Only when Green and associates demonstrated in the late 1970s that pre-adipose 3T3 cells injected into athymic mice developed into mature pads of fat did the concept begin to gain widespread acceptance. (fsu.edu)
  • tissues
  • The following decades have proved them largely right, although the repertoire for studying the organization and behavior of cells within tissues has vastly expanded. (wikipedia.org)
  • This cell culture strategy, termed coculture, induced MDCK acini to undergo branching morphogenesis, in which cells rearrange into a network of interconnected tubules that resembles the development of many tissues. (wikipedia.org)
  • genes
  • The transcription of several G1/S genes is essential for cells to proceed through the cell cycle. (wikipedia.org)
  • They form characteristic cell clusters in suspension culture that express a set of genes associated with pluripotency and can differentiate into endodermal, ectodermal and mesodermal cells both in vitro and in vivo. (wikipedia.org)
  • Line
  • previous to studies of the 3T3 line, it was believed that these characteristics were necessarily synonymous with one another. (fsu.edu)
  • 3T3 cells come from a cell line established in 1962 by two scientists then at the Department of Pathology in the New York University School of Medicine, George Todaro and Howard Green. (wikipedia.org)
  • Madin-Darby Canine Kidney (MDCK) cells are a model mammalian cell line used in biomedical research. (wikipedia.org)
  • This hinted for the first time that the cell line would respond to 3D environments by self-organizing into the appropriate 3D structure reminiscent of kidney tubules. (wikipedia.org)
  • tumors
  • At the time of their establishment, 3T3 cells were different than most other cell lines in regard to the fact they did not induce tumors to develop when injected into murine species. (fsu.edu)
  • expression
  • TCTP knockdown in primary mammary tumor cells, results in increased p53 expression and a decreased number of stem-like cancer cells. (wikipedia.org)
  • These transcription factors promote Cln1/2 expression, and enhance the cell cycle response by forming a positive feedback loop, as Cln1/2 promotes SBF activation and Whi5 export. (wikipedia.org)
  • serum
  • Like the response to mating pheromones in Hartwell's experiments, the response to serum starvation was not uniform amongst all cells. (wikipedia.org)
  • mDia1 localizes to cells' mitotic spindle and midbody, plays a role in stress fiber and filopodia formation, phagocytosis, activation of serum response factor, formation of adherens junctions, and it can act as a transcription factor. (wikipedia.org)
  • He first realised that the addition of bovine fetal calf serum (FCS) to Swiss 3T3 cells increased the polymerisation of actin and assembly of stress fibres. (wikipedia.org)
  • Gene
  • Further DNA testing showed that the transforming sequences in the two cancer cell lines were the same, and the gene was later characterised as N-ras, a member of the Ras gene family. (wikipedia.org)
  • collagen
  • A fibroblast is a large, flat, spindle-shaped cell that is extremely important for wound healing since it produces tropocollagen, the precursor of collagen. (fsu.edu)
  • In the following years, the culture of MDCK cells embedded fully in collagen was shown to yield hollow spheres, or acini. (wikipedia.org)
  • Alexa Fluor
  • In addition, the cells were simultaneously probed for DNA with the ultraviolet-absorbing probe DAPI, and for the cytoskeletal filamentous actin network with Alexa Fluor 488 conjugated to phalloidin. (fsu.edu)
  • The 3T3 cell presented in the digital image above was part of an adherent culture stained for F-actin with Alexa Fluor 568 conjugated to phalloidin, and for DNA with 4',6-diamidino-2-phenylindole ( DAPI ). (fsu.edu)
  • The cells were simultaneously stained with Alexa Fluor 488 conjugated to concanavalin A, a lectin displaying very high affinity for specific carbohydrate residues in glycoproteins, enzymes, and cell membranes. (fsu.edu)
  • In addition, the cells were also stained with Alexa Fluor 488 conjugated to phalloidin (filamentous actin) and MitoTracker Red CMXRos (mitochondria). (fsu.edu)
  • tumor cells
  • Down-regulation of TCTP can induce tumor reversion, and in combination with some drugs that decrease the level of TCTP and will lead to kill tumor cells. (wikipedia.org)
  • Moreover, a recent study with various malignant tumor cells showed that celecoxib could inhibit the growth of these cells, even though some of these cancer cells didn't even contain COX-2. (wikipedia.org)
  • However, when the ability of all these compounds to kill tumor cells in cell culture was investigated, it turned out that the antitumor potency did not at all depend on whether or not the respective compound could inhibit COX-2, showing that inhibition of COX-2 was not required for the anticancer effects. (wikipedia.org)
  • One of these compounds, 2,5-dimethyl-celecoxib, which entirely lacks the ability to inhibit COX-2, actually turned out to display stronger anticancer activity than celecoxib itself and this anticancer effect could also be verified in highly drug-resistant tumor cells and in various animal tumor models. (wikipedia.org)
  • actin
  • The comparison with the normal cells showed that Rac1 stimulates actin filament production at the membrane, pinocytosis, and membrane ruffling. (wikipedia.org)
  • inhibit
  • This bacterium can be effectively treated with β-lactam antibiotics, which inhibit cell wall synthesis. (wikipedia.org)
  • polarity
  • Rho/Rac proteins are involved in a wide variety of cellular functions such as cell polarity, vesicular trafficking, the cell cycle and transcriptomal dynamics. (wikipedia.org)
  • Before cells can undergo key processes such as budding, mitosis, or locomotion, it must have some manner of cell polarity. (wikipedia.org)
  • One example of Rho GTPases' role in cell polarity is seen in the much-studied yeast cell. (wikipedia.org)
  • During the same period in the mid 1980s, a monoclonal antibody was reported by the group of Walter Birchmeier to disrupt cell-cell contacts and alter the front-rear polarity of cells in culture. (wikipedia.org)
  • grown
  • They based this conclusion on the fluid transport activities of monolayers formed of MDCK cells, the presence of microvilli on their apical (upper) surface, and their ability to self-organize, when grown in 3D, into hollow spheres. (wikipedia.org)
  • nucleus
  • The culture was counterstained for DNA in the cell nucleus with DAPI. (microscopyu.com)
  • In eukaryotes, the nuclear envelope encloses the chromosomes (DNA) and other organelles, such as the nucleoli, to clearly define the nucleus of the cell. (fsu.edu)
  • The process involves sucking out the nucleus of a somatic (body) cell and injecting it into an oocyte that has had its nucleus removed Using an approach based on the protocol outlined by Tachibana et al. (wikipedia.org)
  • adipocytes
  • OGT is involved in the resistance of insulin in muscle cells and adipocytes by inhibiting the Threonine 308 phosphorylation of AKT1, increasing the rate of IRS1 phosphorylation (at Serine 307 and Serine 632/635), reducing insulin signaling, and glycosylating components of insulin signals. (wikipedia.org)
  • undergo
  • However, due to examination of 3T3 cells and subsequent research it has become widely accepted that for immortalization of cells to take place, telomere shortening, which can instigate chromosomal rearrangements, must be overcome, a process that is not necessarily related to a cell s ability to undergo oncogenic transformation. (fsu.edu)
  • synthesis
  • Hartwell not only identified the mutant, cdc28, which arrests in very early stages of the cell cycle, but he also recognised that the presence of mating factors could result in similar phenotypes of inhibited bud formation and lack of DNA synthesis. (wikipedia.org)
  • COX-1 is responsible for the synthesis of prostaglandin and thromboxane in many types of cells, including the gastro-intestinal tract and blood platelets. (wikipedia.org)
  • kidney
  • Indeed, they chose to isolate kidney tubules with precisely this goal in mind, as they had previously succeeded with viral infection of cells derived from kidney tubules from other mammals. (wikipedia.org)
  • cytoskeletal
  • All G proteins are "molecular switches", and Rho proteins play a role in organelle development, cytoskeletal dynamics, cell movement, and other common cellular functions. (wikipedia.org)
  • cellular
  • screened for and characterized temperature sensitive mutants, also known as cell division cycle mutants (cdc mutants), that display arrested cellular development at various stages of the cycle. (wikipedia.org)
  • Only postmitotic cells younger than three hours arrested cellular division in these conditions, while cells older than four hours were insensitive to the absence of growth factors. (wikipedia.org)
  • His work, in collaboration with his colleague and close friend Christopher Marshall, made seminal contributions to our understanding of cell signalling in animal cells, in particular the role of Rho and Ras small GTPases in regulating a variety of cellular functions such as proliferation, morphology and migration. (wikipedia.org)
  • The researchers were able to identify the minimal conditions and factors that would be sufficient for starting the cascade of molecular and cellular processes to instruct pluripotent cells to organize the embryo. (wikipedia.org)
  • 1924
  • In 1924 Spemann and Mangold demonstrated the key importance of cell-cell inductions during animal development. (wikipedia.org)
  • Jean Lindenmann (September 18, 1924 - January 15, 2015) was a Swiss virologist and immunologist. (wikipedia.org)
  • Lindenmann was born on September 18, 1924, in Zagreb, Yugoslavia (present-day Croatia), to a Swiss father and a French mother from Paris. (wikipedia.org)
  • mitosis
  • These experimental results show strong evidence for a commitment point to enter mitosis, and consequently suggest that the cell is capable of sensing its environment for cues like growth factors before committing. (wikipedia.org)
  • Biology
  • Alan Hall FRS (19 May 1952 - 3 May 2015) was a British cell biologist and a biology professor at the Sloan-Kettering Institute, where he was chair of the Cell Biology program. (wikipedia.org)
  • In 1993 he moved to University College London, where he helped to create a new MRC centre for molecular cell biology. (wikipedia.org)
  • progression
  • The export of Whi5 results in the partial activation of the transcription factors SBF and MBF, which ultimately promote cell cycle progression. (wikipedia.org)
  • A modern day study delineating the relationship between mating arrest and cell cycle progression was put forth by Doncic et al. (wikipedia.org)
  • formation
  • One of the most obvious changes to cell morphology controlled by Rho proteins is the formation of lamellipodia and filopodia, projecting processes that look like "fingers" or "feet" and often propel cells or growth cones across surfaces. (wikipedia.org)
  • motility
  • These results suggest that inhibitory effects of melittin on breast cancer cell motility and migration may be related to the inhibition of mTOR pathway. (blogspot.co.nz)
  • MDCK cells are used for a wide variety of cell biology studies including cell polarity, cell-cell adhesions (termed adherens junctions), collective cell motility, as well as responses to growth factors. (wikipedia.org)
  • Over the same period in the 1980s, biologists studying cell motility had hit upon an interesting and reproducible behavior of cells in culture: the scattering response. (wikipedia.org)
  • These disparate observations eventually coalesced into a resilient paradigm for cell motility and cell polarity. (wikipedia.org)
  • Beyond that immediate paradox, a crucial connection was forged between the acute induction of cell motility in 2D culture by the "scatter factor", and its impact on the spatial organization adopted by tissues in 3D. (wikipedia.org)
  • This connection remains significant as a link between precisely defined mechanisms of cell motility in 2D and complex rearrangements in 3D whose regulation is yet to be understood fully. (wikipedia.org)
  • embryo
  • This Histone H3 K18ac antibody is useful for ChIP, Immunocytochemistry/Immunofluorescence, Dot Blot, and Western Blot where a band is seen ~15kDa in HeLa histone prep, NIH 3T3 histone prep, and C. elegans embryo lysate. (novusbio.com)
  • Normally, only stem cells and progenitor cells express MET, which allows these cells to grow invasively in order to generate new tissues in an embryo or regenerate damaged tissues in an adult. (wikipedia.org)
  • The researchers were able to identify the minimal conditions and factors that would be sufficient for starting the cascade of molecular and cellular processes to instruct pluripotent cells to organize the embryo. (wikipedia.org)
  • Todaro and Green originally obtained their 3T3 cells from Swiss albino mouse embryo tissue. (wikipedia.org)
  • Tissue
  • IL-8, C5a, and leukotriene B 4 (LTB 4 )) 3 that are secreted by several tissue cells in response to a physical or chemical insult, or by certain bacterial products, such as LPS or FMLP, during infection ( 13 , 14 ). (jimmunol.org)
  • Therefore, the aim of the study was to establish a cell culture of the porcine cervical epithelium representing in vivo -like properties of the tissue. (beds.ac.uk)
  • We could reproducibly culture pure epithelial cells from cervical tissue explants. (beds.ac.uk)
  • To elucidate cell type-specific actions of hormones and cytokines, signal transduction pathways, cell-cell interactions, and gene expression in these highly specialized cells, model systems resembling the original tissue need to be developed. (beds.ac.uk)
  • The cells used in these studies are mainly derived from human ectocervical tissue, which in vivo is covered by a polarized, multilayered epithelium. (beds.ac.uk)
  • However, the cells (primary, immortalized or transformed) are cultured as monolayers and therefore lost these tissue specific characteristics. (beds.ac.uk)
  • Thus the initial goal in isolating and culturing cells from this tissue was not to generate a new model system for epithelial cell biology. (wikipedia.org)
  • In their report, the authors speculated that the "histotypic expression" by which MDCK cells formed structures reminiscent of their tissue of origin might be fruitfully applied to the study of other tissues. (wikipedia.org)
  • Through the 1970s, the MDCK cell line found new use as a model for mammalian epithelial tissue. (wikipedia.org)
  • This work solved an outstanding mystery of MDCK culture, as the tissue from which these cells were derived is tubular, yet they had previously only developed into spherical acini in 3D culture. (wikipedia.org)
  • After injury, mature terminally differentiated kidney cells dedifferentiate into more primordial versions of themselves and then differentiate into the cell types needing replacement in the damaged tissue Macrophages can self-renew by local proliferation of mature differentiated cells. (wikipedia.org)
  • In newts, muscle tissue is regenerated from specialized muscle cells that dedifferentiate and forget the type of cell they had been. (wikipedia.org)
  • Expression
  • Pre-clinical data arising from comparison between non-HPV-tumor cells and their counterparts transfected with sequences of HPV genome should be interpreted with caution because artificial induced expression might not mirror in vivo reality. (biomedcentral.com)
  • In addition, the endotoxin-induced expression of the inducible nitric oxide synthase (iNOS) and cyclooxygenase (COX)-2 proteins was investigated in a mouse macrophage cell line (RAW 264.7) treated with ACE in vitro, to clarify the anti-inflammatory effect. (arvojournals.org)
  • By contrast, the expression of parvalbumin in the cytosol did not alter cell proliferation or cell cycle distribution. (biologists.org)
  • Most interestingly, the expression of the parvalbumin in the nucleus, but not in the cytosol, reduced the development of tumours when HepG2 cells were subcutaneously implanted into nude mice. (biologists.org)
  • On the other hand, the expression of an homologous gene, the GTPase regulator associated with the focal adhesion kinase 1/Oligophrenin-1-like (GRAF1/OPHN-1-L), was relatively high in blood mononuclear cells and significantly decreased in ATRX patients. (biomedcentral.com)
  • These transcription factors promote Cln1/2 expression, and enhance the cell cycle response by forming a positive feedback loop, as Cln1/2 promotes SBF activation and Whi5 export. (wikipedia.org)
  • transcription
  • Inhibition of the MAPK- but not the PtdIns3K-dependent pathway inhibited NF-kappaB signaling, and further, CD1 transcription and cell cycle progression. (nih.gov)
  • mDia1 localizes to cells' mitotic spindle and midbody, plays a role in stress fiber and filopodia formation, phagocytosis, activation of serum response factor, formation of adherens junctions, and it can act as a transcription factor. (wikipedia.org)
  • The export of Whi5 results in the partial activation of the transcription factors SBF and MBF, which ultimately promote cell cycle progression. (wikipedia.org)
  • HeLa
  • Immunocytochemistry/ Immunofluorescence: PKM2 Antibody [NBP1-- Immunocytochemical analysis of PKM2 in HeLa cells. (novusbio.com)
  • proliferation
  • Collectively, these data suggest that nuclear Ca 2+ signals are important for regulating cell proliferation in some contexts. (biologists.org)
  • Nuclear factor kappa B (NF-kappaB) has been implicated in the regulation of cell proliferation and transformation. (nih.gov)
  • His work, in collaboration with his colleague and close friend Christopher Marshall, made seminal contributions to our understanding of cell signalling in animal cells, in particular the role of Rho and Ras small GTPases in regulating a variety of cellular functions such as proliferation, morphology and migration. (wikipedia.org)
  • cervical cells
  • 1989[ 11 ] to evaluate the influence of E2 on intrinsic radiosensitivity of cervical cells to support the hypothesis of E2-gene status as a predictive marker for therapeutic outcome in cervical cancer patients. (biomedcentral.com)
  • Primary porcine cervical cells performed best in conditioned Ham's F-12, containing 10% FCS, EGF and insulin. (beds.ac.uk)
  • translocation
  • Nuclear translocation is sufficient to induce cell death that is caspase independent, isoform-specific and independent of its enzymatic actvity. (novusbio.com)
  • Treatment with the calcium chelator led to a drastic inhibition in the level of p65 translocation in comparison to control cells (Fig. 4 B). The ratio of the nuclear/cytoplasmic fluorescence intensities reached a maximum of 1.6 in control conditions versus 0.4 in presence of BAPTA. (nih.gov)
  • Nucleus
  • It has been well established that Ca 2+ signals occurring inside cells affect activities within the nucleus. (biologists.org)
  • The process involves sucking out the nucleus of a somatic (body) cell and injecting it into an oocyte that has had its nucleus removed Using an approach based on the protocol outlined by Tachibana et al. (wikipedia.org)
  • scientists
  • Swiss scientists are using a special inkjet printer to assemble three dimensional living constructs that resemble living tissues. (blogspot.com)
  • By studying the connections and communications taking place between cells and between cells and ECM molecules, the scientists were able to reconstruct this matrix and thus create a new kind of biological ink. (blogspot.com)
  • 3T3 cells come from a cell line established in 1962 by two scientists then at the Department of Pathology in the New York University School of Medicine, George Todaro and Howard Green. (wikipedia.org)
  • progenitor
  • They are classified as either totipotent (iTC), pluripotent (iPSC) or progenitor (multipotent-iMSC, also called an induced multipotent progenitor cell-iMPC) or unipotent-(iUSC) according to their developmental potential and degree of dedifferentiation. (wikipedia.org)
  • cycle progression
  • The response to mating pheromones as described in Hartwell's experiments is unsurprising considering the antagonistic biochemical interactions between the mating pathway and the G1 cyclins that promote cell cycle progression. (wikipedia.org)
  • A modern day study delineating the relationship between mating arrest and cell cycle progression was put forth by Doncic et al. (wikipedia.org)
  • Nuclear
  • Do patterns of nuclear Ca 2+ signalling simply track cytosolic Ca 2+ signalling, or are there independent nuclear Ca 2+ signalling mechanisms that are necessary for proper cell function? (biologists.org)
  • Induced totipotent cells can be obtained by reprogramming somatic cells with somatic-cell nuclear transfer (SCNT). (wikipedia.org)
  • behavior
  • The following decades have proved them largely right, although the repertoire for studying the organization and behavior of cells within tissues has vastly expanded. (wikipedia.org)
  • serum starvation
  • 24 h after serum starvation, the cells were either stimulated with serum alone (filled squares) or pretreated for 10 min with 10 μM BAPTA-AM (open circles). (nih.gov)
  • Like the response to mating pheromones in Hartwell's experiments, the response to serum starvation was not uniform amongst all cells. (wikipedia.org)
  • proteins
  • Rho proteins help cells regulate changes in shape throughout their life-cycle. (wikipedia.org)
  • One of the most obvious changes to cell morphology controlled by Rho proteins is the formation of lamellipodia and filopodia, projecting processes that look like "fingers" or "feet" and often propel cells or growth cones across surfaces. (wikipedia.org)
  • Involvement
  • Despite this evidence implicating the involvement of the upstream members of the PI3K pathway, the role of downstream links such as mammalian target of rapamycin (mTOR) and ribosomal p70 S6 kinase (p70S6K) has not been examined directly in hemopoietic cells. (jimmunol.org)
  • culture
  • this research will improve cell culture and then will eventually be used as a base for creating tissues. (blogspot.com)
  • The cells were suspended in culture medium supplemented with 0.8% wt. (blogspot.com)
  • We tested different isolation methods and culture conditions and validated purity of the cultured cells by immunohistochemistry against keratins. (beds.ac.uk)
  • Cell culture models used in basic research as well as in toxicology ideally should meet two requirements at the same time: to a) be easily and continuously available and b) resemble the in vivo properties of the specific cell type. (beds.ac.uk)
  • Therefore, we investigated, if porcine material from the slaughterhouse could provide to establish a suitable and differentiated cell culture model of the uterine cervical epithelium. (beds.ac.uk)
  • It is one of few cell culture models that is suited for 3D cell culture and multicellular rearrangements known as branching morphogenesis. (wikipedia.org)
  • In the following years, the culture of MDCK cells embedded fully in collagen was shown to yield hollow spheres, or acini. (wikipedia.org)
  • Epithelial cells in culture grow normally as tight clusters. (wikipedia.org)
  • This cell culture strategy, termed coculture, induced MDCK acini to undergo branching morphogenesis, in which cells rearrange into a network of interconnected tubules that resembles the development of many tissues. (wikipedia.org)
  • The spontaneously immortalized cells with stable growth rate were established after 20 to 30 generations in culture, and then named '3T3' cells. (wikipedia.org)
  • Centre
  • In 1993 he moved to University College London, where he helped to create a new MRC centre for molecular cell biology. (wikipedia.org)
  • viral
  • In 1957, Lindenmann and Isaacs discovered that the cells exposed to the dead viruses secreted a previously unknown substance which blocked future viral infections, which became known as interferon. (wikipedia.org)
  • growth
  • Cell Growth Differ. (wikipedia.org)
  • 3T3 cells are often used in the cultivation of keratinocytes, with the 3T3 cells secreting growth factors favourable to these kinds of cells. (wikipedia.org)
  • These experimental results show strong evidence for a commitment point to enter mitosis, and consequently suggest that the cell is capable of sensing its environment for cues like growth factors before committing. (wikipedia.org)
  • The activity of Cln3 correlates with cell growth rate. (wikipedia.org)
  • molecular
  • Chemistry: molecular biology and microbiology animal cell, per se (e.g., cell lines, etc. (patentsencyclopedia.com)
  • Easily accessible, differentiated model systems of the cervical epithelium are not yet available to elucidate the underlying molecular mechanisms within these highly specialized cells. (beds.ac.uk)
  • molecule
  • The mechanism of GM-CSF-induced chemotaxis appears to involve the cell signaling molecule, ribosomal p70 S6 kinase (p70S6K). (jimmunol.org)
  • physiological
  • Cervical uterine epithelial cells maintain a physiological and pathogen-free milieu in the female mammalian reproductive tract and are involved in sperm-epithelium interaction. (beds.ac.uk)