Sv40 cells. Medical search. Frequent questions

SV40 large T antigen (Simian Vacuolating Virus 40 TAg) is a hexamer protein that is a dominant-acting oncoprotein derived from the polyomavirus SV40. (wikipedia.org)
From 1955 to 1963, an estimated 10-30% of polio vaccines administered in the US were contaminated with simian virus 40 (SV40). (cdc.gov)
The health regulator says Pfizer did not disclose the presence of the Simian Virus 40 (SV40) DNA sequence in its mRNA COVID-19 vaccine at the time of filing. (theepochtimes.com)
Health Canada has confirmed the presence of a Simian Virus 40 (SV40) DNA sequence in the Pfizer COVID-19 vaccine, which the manufacturer had not previously disclosed. (theepochtimes.com)
13] In fact, in 1958, a scientific journal reported that the rate of isolation of new simian viruses (from monkey kidney cells) has continued unabated. (ronpaulforums.com)
But one of the study's authors told PolitiFact this is not the same as simian virus 40, or SV40, which can cause tumors in monkeys. (politifact.com)
Simian virus 40, or SV40, is a tumor-causing virus found in monkeys. (politifact.com)
As a post-doctoral fellow with Paul Berg, I developed a subcellular system that allowed simian virus 40 (SV40) DNA to continue replication in vitro. (nih.gov)

SV40 Early Region and Large T Antigen in Human Brain Tumors, Peripheral Blood Cells, and Sperm Fluids from Healthy Individuals. (aacrjournals.org)
SV40 DNA replication is initiated by binding of large T-antigen to the origin region of the genome. (wikipedia.org)
The function of T-antigen is controlled by phosphorylation, which attenuates the binding to the SV40 origin. (wikipedia.org)
The SV40 large T-antigen has been used as a model protein to study nuclear localization signals (NLSs). (wikipedia.org)
The Immortalized Human Lens Epithelial Cells react positively with the monoclonal antibody to SV40 large T antigen. (accegen.com)
Murine lung epithelial (MLE) cell lines were produced from lung tumors derived from transgenic mice bearing the viral oncogene, SV40 large T antigen, under transcriptional control of the promoter-enhancer region of the human surfactant protein C (SP-C) gene. (elsevierpure.com)
SV40 T antigen transforms calcitonin cells of the thyroid but not CGRP-containing neurons in transgenic mice. (harvard.edu)
The aim of this work is to characterize some of the events associ ated with the Joss of proliferative capacity in a rat cell line, called REtsAF, which is conditionally immortalized by the tsA58 allele of SV 40 large T antigen. (uvsq.fr)
It's important for the public to realize that the SV40 sequence in the vaccine is NOT the cancer-causing SV40 large T antigen, which would be a very significant cancer risk," Dr. Buckhaults told The Epoch Times via email. (theepochtimes.com)
The directed expression of SV40 T antigen has led to the development of several important transgenic models with spontaneous epithelial tumor formation. (molvis.org)
Immunohistochemical studies of biopsy specimens or cell block specimens may indicate the presence of at least two mesothelial markers: calretinin, cytokeratin 5/6, Wilm's tumor 1=antigen or D2-40. (standardofcare.com)
Immune checkpoint Inhibitors Nivolumab, and ipililumab targeting programmed cell death 1 and cytotoxic T-lymphocyte antigen 4 have shown superiority as frontline treatment as compared to standard of care chemotherapy: survival 18.1 months versus 14.1 months. (standardofcare.com)
After testing several different genes, they were able to improve reprogramming efficiency by adding a viral protein known as SV40 large T antigen. (hopkinsmedicine.org)
Using both fetal and adult human skin cells, the researchers introduced the four genes previously reported sufficient for cell reprogramming and compared the efficiency of reprogramming in the presence or absence of large T antigen. (hopkinsmedicine.org)
The part of the SV40 virus that can potentially cause cancer, known as the T-antigen, is not present in the COVID-19 vaccine, Buckhalts said. (politifact.com)

Tumors rarely, if ever, arise from neurons in the adult, although they do from other cells from the same lineage, such as the neuroendocrine C cells of the thyroid. (harvard.edu)
SV40 and human tumors: myth, association or causality? (nature.com)
Given the high frequency of DDX5 amplification in breast cancer, our results highlight DDX5 as a promising candidate for targeted therapy of breast tumors with DDX5 amplification, and indeed we show that DDX5 inhibition sensitizes a subset of breast cancer cells to trastuzumab. (aacrjournals.org)
Furthermore, growth of orthotopically implanted tumors in vivo is inhibited by blocking PVRL4-driven cell-to-cell attachment with monoclonal antibodies, demonstrating a novel strategy for targeted therapy of cancer. (elifesciences.org)
It is estimated that up to 90% of cancers in humans originate in epithelial tissue, and the cells within such tumors are known to survive and divide even when they are no longer attached to the extracellular matrix. (elifesciences.org)
Normally, the PVRL4 gene is not active in breast epithelial cells, but its activity is detected in many breast, lung, and ovarian tumors. (elifesciences.org)
This behavior is particularly evident in the cells that divide aggressively to form tumors that subsequently migrate and colonize other tissues around the body. (elifesciences.org)
used genetic techniques to silence PVRL4 in cells from breast tumors, they found that it reduced the formation of clusters by the cancer cells and also reduced their ability to grow in the absence of attachment. (elifesciences.org)

TAg is a product of an early gene transcribed during viral infection by SV40, and is involved in viral genome replication and regulation of host cell cycle. (wikipedia.org)
After entering the cell, the viral genes are transcribed by host cell RNA polymerase II to produce early mRNAs. (wikipedia.org)
Therefore, it is essential for the host cell to enter S phase, when cell DNA and the viral genome are replicated together. (wikipedia.org)
Viral culture is also related, with cells as hosts for the viruses. (wikipedia.org)
In addition to the viral particles, you will also receive purified pAAV.CAG.Flex.GCaMP6s.WPRE.SV40 plasmid DNA. (addgene.org)
Performance of the LV-MAX Lentiviral Production System was shown to be equivalent to the CTS LV-MAX production system as measured by viral titers determined by transduction of HT1080 cells and flow cytometry analysis of GFP-positive cells. (thermofisher.com)
As more cell and gene therapies enter into the clinical and commercial space, there is an emerging need for more cost-effective and scalable viral vector manufacturing platforms, such as lentiviral vector production. (thermofisher.com)
Drugs that block DNA replication can arrest the spread of cancer cells and eliminate viral pathogens. (nih.gov)
In the past, our research focused on viral genomes as models for DNA replication in mammalian cell nuclei. (nih.gov)
We used isolated nuclei from virus infected cells supplemented with cytoplasm, and discovered that viral replicating chromosomes could continue replication in the absence of a nucleus. (nih.gov)

These cells also provide a unique system for examining the relationship between gene expression and differentiation. (accegen.com)
Western blot and precipitation are used to confirm the presence of βH- and γ-crystallin, the proteins that are markers for cell differentiation in the lens epithelium. (accegen.com)
This suggests that as part of the terminal differentiation process of these neurons, the cessation of mitosis is accompanied by a relative refractoriness to oncogenes that may transform other cells of the same lineage. (harvard.edu)
Microinjection of the ras oncogene protein into PC12 cells induces morphological differentiation. (cshlpress.com)
β -Catenin is a transcription factor that plays an important role in cell proliferation, cellular differentiation, apoptosis, and metastasis [ 10 ]. (hindawi.com)
Sequential rounds of division and differentiation give rise to an enormous amplification of cell numbers, necessary for the production of mature blood cells. (justia.com)
This process of division and differentiation is subject to regulation at many levels to control cell production. (justia.com)

Accordingly, Rad51b-c.92delT variant reduced replication fork progression of patient-derived lymphoblastoid cell lines and pluripotent reprogramming efficiency of primary mouse embryonic fibroblasts. (nature.com)
SV40 immortalized mouse embryonic fibroblasts derived from Bax-Bak double-knockout mice were significantly more resistant to GL-induced cell killing compared with wild-type cells. (aspetjournals.org)

SV40 large TAg, other polyomavirus large T antigens, adenovirus E1a proteins, and oncogenic human papillomavirus E7 proteins share a structural motif that encodes a high-affinity pRb-binding domain. (wikipedia.org)
My laboratory has developed new technologies and applied them towards understanding the molecular biology and enzymology of DNA replication in animal cells and viruses (SV40, polyomavirus, papillomavirus, and herpes simplex virus), and at the beginning of animal development (mouse preimplantation embryos and frog eggs). (nih.gov)
We were among the first to develop and exploit sub-cellular systems that allowed SV40 and polyomavirus to complete DNA replication in vitro. (nih.gov)

The virus came from monkey kidney cell cultures used to make polio vaccines at that time. (cdc.gov)
After the contamination was discovered, the U.S. government established testing requirements to verify that all new lots of polio vaccines were free of SV40. (cdc.gov)
Mr. McKernan, a former researcher and team leader for the Massachusetts Institute of Technology Human Genome Project, told The Epoch Times he suspects Pfizer didn't disclose the presence of the DNA sequence due to the association of SV40 with polio vaccines. (theepochtimes.com)
Polio vaccines used in the late 1950s and early 1960s were found to be contaminated with SV40 as the virus was present in monkey kidney cells that were used to grow the vaccine. (theepochtimes.com)

For example, circRNA_000166 can function as an oncogene by promoting the proliferation of CRC cells and by limiting apoptosis. (hindawi.com)

Growing viruses in cell cultures allowed preparation of purified viruses for the manufacture of vaccines . (wikipedia.org)
No vaccines used today contain SV40 virus. (cdc.gov)
The regulator said that after scientists Kevin McKernan and Dr. Phillip J. Buckhaults publicly raised the presence of SV40 enhancers in the vaccines earlier this year, "it was possible for Health Canada to confirm the presence of the enhancer based on the plasmid DNA sequence submitted by Pfizer against the published SV40 enhancer sequence. (theepochtimes.com)
Dr. Patrick Provost, a professor in the Department of Microbiology, Infectious Diseases and Immunology at the Faculty of Medicine at Laval University, said the SV40 enhancer sequence was used in the vaccines because it's a component of the plasmid used for amplification of the vaccine mRNA. (theepochtimes.com)
He said that since the average length of the degraded DNA fragments found by Dr. Buckhaults was 100 base pairs, and the SV40 enhancer sequence is only 72 base pairs long, the chances of finding an intact, fully functional SV40 enhancer sequence in the mRNA vaccines is "relatively high, certainly not negligible. (theepochtimes.com)
Another issue Dr. Provost raised is the contaminating DNA fragments in the vaccines likely being encapsulated in lipid nanoparticles, which could facilitate their delivery into human cells. (theepochtimes.com)
Monkey DNA and the SV40 virus also are not listed among the ingredients in the COVID-19 vaccines that federal regulators have approved for use. (politifact.com)
Monkey DNA and SV40 are not listed among the ingredients for the COVID-19 vaccines manufactured by Pfizer-BioNTech, Moderna, Johnson & Johnson and Novavax, according to the Centers for Disease Control and Prevention. (politifact.com)
The study tested four expired vials of the Moderna and Pfizer COVID-19 vaccines and found elements of the virus, including an "SV40 promoter" in the Pfizer vaccine vials. (politifact.com)
However, McKernan said they did not find the whole SV40 virus in the vaccines. (politifact.com)

The Immortalised Lens Epithelial Cells - SV40 is established from primary cells (isolated from infant lens epithelial fragments) using Adl2-SV40 adenovirus. (accegen.com)
The Immortalised Human Lens Epithelial Cells - SV40 provides a homogeneous population of cell for the investigation of the physiology of the normal human lens epithelium and study its response to cataractogenic agents and the human lens specific functions. (accegen.com)
This mechanism preserves the single-cell layer pattern throughout the body and prevents epithelial cells from growing in inappropriate locations. (elifesciences.org)
explored which genes enable epithelial cells from the human mammary gland to grow without being attached to the extracellular matrix. (elifesciences.org)

Although there is no evidence of SV40 sequences in OPV, the Expert Committee agreed to the introduction of a gene amplification test for SV40 in poliovirus seed stocks to provide an additional level of security. (who.int)
Because his goal was to create a live attenuated vaccine, Dr. Sabin had to isolate the poliovirus strains and then passage the strains through a myriad of host cells in order to attain the right virulence strong enough to illicit an immune response, but weak enough so as to not cause polio in the recipient. (ronpaulforums.com)
10] A small quantity of poliovirus could be added to the minced kidneys surgically removed from these monkeys and within a few days, large quantities of poliovirus could then be harvested from these same monkey cells. (ronpaulforums.com)
11] When the poliovirus was passaged through the monkeys or grown on the monkey kidney cells for production, extraneous viruses became part of the final poliovirus vaccine. (ronpaulforums.com)
A gain of function screen for genes that enable proliferation independently of matrix anchorage identified a cell adhesion molecule PVRL4 (poliovirus-receptor-like 4), also known as Nectin-4. (elifesciences.org)
They found that the gene that codes for a protein called poliovirus receptor-like 4 (PVRL4) allows attachment-free cell growth and also makes cells cluster together once detached from the matrix. (elifesciences.org)

A single-cell layer of epithelium is separated from the tissues beneath it by a supporting substance called the extracellular matrix. (elifesciences.org)

Health Canada expects sponsors to identify any biologically functional DNA sequences within a plasmid (such as an SV40 enhancer) at the time of submission," the agency said in an email to The Epoch Times. (theepochtimes.com)
Newly developed gene amplification tests can also detect noninfectious SV40 sequences. (who.int)
Noteworthy, was our demonstration that although termination of DNA replication did not require specific DNA sequences, some DNA sequences did promote pausing of DNA replication forks in vivo (and DNA polymerase in vitro), and some sequences, such as thosewithin the termination region for SV40 DNA replication, did promote formation of catenated intertwines during separation of sibling chromosomes. (nih.gov)

Because of the relative simplicity of the genome, polyomaviruses are heavily dependent on the cell for transcription and genome replication. (wikipedia.org)

We have found that 2 kb of the calcitonin gene-related peptide (CGRP)/calcitonin gene suffices to target expression to CGRP-containing neurons, such as those in the dorsal root ganglia (DRG), and to the calcitonin-secreting C cells of the thyroid. (harvard.edu)
As the world leader in serving science, Thermo Fisher Scientific delivers innovative solutions to accelerate the development of lentivirus production to help advance the field of cell and gene therapy. (thermofisher.com)
With solutions that span the entire LV workflow, we provide high-quality products and expertise to help companies develop breakthrough cell and gene therapies. (thermofisher.com)
However, the two share different degrees of concern about the significance of an SV40 sequence-which is used as an enhancer to drive gene transcription during the vaccine manufacturing process-being present in the shots. (theepochtimes.com)
Examination for the gene structure of the green monkey cells prove that it is not genetically possible to transfer the AIDS virus from monkeys to man by natural means. (beyondweird.com)
On account of the persistence of DNA in all environments, and the ability of practically all cells to take up 'naked' or free DNA, the success of horizontal gene transfer may depend largely on the nature of the DNA itself. (i-sis.org.uk)
In the SV40 virus, the SV40 promoter drives the expression of a cancer-causing gene, said Phillip Buckhalts, director of the Cancer Genetics Lab and professor at the University of South Carolina, who was not involved in the study. (politifact.com)
Here we identify CSNK1E , the gene encoding casein kinase 1 epsilon (CK1ε) as required specifically for the proliferation of breast cancer cells with activated β-catenin and confirm its role as a positive regulator of β-catenin-driven transcription. (plos.org)

Tissue culture commonly refers to the culture of animal cells and tissues, with the more specific term plant tissue culture being used for plants. (wikipedia.org)
[12] He suggested that the potentialities of individual cells via tissue culture as well as that the reciprocal influences of tissues on one another could be determined by this method. (wikipedia.org)
Lundstig A, Dejmek A, Eklund C, Filinic I, Dillner J. No detection of SV40 DNA in mesothelioma tissues from a high incidence area in Sweden. (cdc.gov)
Understanding how cancerous cells gain this ability may lead to new approaches to stopping tumor cells from dividing and colonizing tissues around the body. (elifesciences.org)
In this present study, we found that circIFT80 was abnormally overexpression in colorectal cancer tissues and tumor cells. (hindawi.com)
This new cell, which is microscopic, will multiply and organize perfectly all distinct tissues to generate a full grown individual with organs and systems as complex as brain and immune system. (bvsalud.org)

Repair of mitomycin-C-induced chromosomal aberrations was impaired in RAD51B/Rad51b-c.92delT human and mouse somatic cells in vitro and in explanted mouse bone marrow cells. (nature.com)
In contrast to glycoproteins secreted from mammalian cells, glycoproteins secreted from baculovirus can be easily de-glycosylated in vitro. (fishersci.com)

Our results show a novel role for DDX5 in cancer cell proliferation and suggest DDX5 as a therapeutic target in breast cancer treatment. (aacrjournals.org)
DDX5 is required for cell proliferation by controlling the transcription of genes expressing DNA replication proteins in cancer cells in which the DDX5 locus is amplified, and this has uncovered a dependence on DDX5 for cell proliferation. (aacrjournals.org)
Defects in the control of cell proliferation are a hallmark of cancer, and DNA replication is a key process for cell proliferation. (aacrjournals.org)
While knockout circIFT80 in HT29 cell or SW480 cells, the proliferation, and migration of the cells were inhibited, the cell cycle was arrested in G2/M phase, and the cell apoptosis was increased. (hindawi.com)
Failure of mammalian cells to regulate their proliferation cycle leads to cancer. (nih.gov)
The Wnt/β-catenin pathway plays a critical role in embryonic development, maintenance of multipotent progenitor cell populations and proliferation of many tissue types [1] , [2] . (plos.org)
Also, our mutants induced proliferation, glucose uptake, inhibited the migration of 293T cells and affected the responsiveness of the cells to cisplatin and radiation. (scielo.br)

SV40 is a double-stranded, circular DNA virus belonging to the Polyomaviridae (earlier Papovavirus) family, Orthopolyomavirus genus. (wikipedia.org)

TAg is capable of inducing malignant transformation of a variety of cell types. (wikipedia.org)
The pRb-binding motif and negatively charged region match to a segment of SV40 TAg beginning at residue 102 and ending at residue 115 as shown below: - Asn - Leu - Phe - Cys - Ser - Glu - Glu - Met - Pro - Ser - Ser - Asp - Asp - Glu - Functional studies of TAg proteins bearing mutations within this segment (amino acid positions 106 to 114, inclusive) demonstrate that certain deleterious mutations abolish malignant transforming activity. (wikipedia.org)
2005). SV40 enhances the risk of malignant mesohelioma among people exposed to asbestos: a molecular epidemiological case-control study. (nature.com)
Malignancies involving mesothelial cells that normally line the body cavities, including the pleura (see the images below), peritoneum, pericardium, and testis, are known as malignant mesothelioma. (medscape.com)

It relies on generation of recombinant virus by site-specific transposition in E. coli (DH10Bac™) rather than homologous recombination in insect cells. (fishersci.com)
Transfect the bacmid into insect cells for fast production of a high titer of pure recombinant baculovirus particles in the very first transfection. (fishersci.com)

The vector uses the strong polyhedrin promoter to generate high levels of expression in a variety of insect cell lines such as Sf9, Mimic™ Sf9, Sf21, and High Five™ cells in Sf-900 II & Sf-900™ III media from Gibco. (fishersci.com)
An Epoch Times article cited an April study, which has not been peer-reviewed, that found part of a DNA sequence called an "SV40 promoter" in two expired Pfizer-BioNTech COVID-19 vaccine vials. (politifact.com)
On its own, the SV40 promoter cannot cause cancer, molecular biology experts told PolitiFact. (politifact.com)
In this case, the "SV40 promoter" is a DNA sequence that was first identified in the SV40 virus. (politifact.com)

These Deadly viruses are " Retro - Viruses ", meaning that they can change the genetic composition of the cells that they enter. (beyondweird.com)
The mechanisms used by certain viruses to dysregulate the host cell through manipulation of epigenetic processes and the role of cellular cofactors such as BRD4 that are known to be involved in epigenetic regulation of host cell pathways will also be covered. (mdpi.com)
Dr. Sabin s strains would be attenuated or weakened by transferring or passaging[2] the live viruses through different host cells and then fed to children orally. (ronpaulforums.com)
Therefore, the overall goal of our work is to discover how DNA replication is regulated both in the large chromosomes of cells and in the "mini-chromosomes" of viruses and small extrachromosomal DNA molecules. (nih.gov)

SV40 was isolated by Sweet and Maurice Hilleman in 1960 in primary monkey kidney cell cultures being used to grow Sabin OPV. (wikipedia.org)
Between 1959 and 1960, Bernice Eddy, Ph.D., of the National Institute of Health (NIH) examined minced rhesus monkey kidney cells under a microscope. (ronpaulforums.com)

Although the full DNA sequence of the Pfizer plasmid was provided at the time of initial filing, the sponsor did not specifically identify the SV40 sequence. (theepochtimes.com)

This vaccine was made possible by the cell culture research of John Franklin Enders , Thomas Huckle Weller , and Frederick Chapman Robbins , who were awarded a Nobel Prize for their discovery of a method of growing the virus in monkey kidney cell cultures. (wikipedia.org)
5] Next, the strain (now called Monk14 T11) underwent fifteen more passages in monkey testicular cultures, eighteen passages in monkey kidney cells, two passages through the skin of living rhesus monkeys, and additional passages through African Green monkey skin and monkey kidney cell cultures. (ronpaulforums.com)
In 1956, Dr. Sabin took this virus and passaged it through seven cultures of African Green Monkey kidney cells. (ronpaulforums.com)

2005). SV40-dependent AKT activity drives mesothelial cell transformation after asbestos exposure. (nature.com)

The SV40 genome is very small and does not encode all the information necessary for DNA replication. (wikipedia.org)
According to Dr. Provost, the danger of the SV40 enhancers being present in the vaccine is their possible integration into a cell's DNA genome. (theepochtimes.com)
13 Genome Plasticity and B Cell, University of Paris-Sud, Centre National de la Recherche Scientifique, Cancer Institute Gustave Roussy, Villejuif, France. (jci.org)

This plasmid was previously available as pAAV.CAG.Flex.GCaMP6s.WPRE.SV40 (p2818) from the Penn Vector Core. (addgene.org)
Dr. Buckhaults, professor of cancer genomics and director of the Cancer Genetics Lab at the University of South Carolina, has tried to alleviate concerns about the presence of the SV40 enhancers, saying they pose a "small [cancer] hazard, but so do all the other pieces of plasmid DNA. (theepochtimes.com)
Dr. Provost said the SV40 enhancer could still be in the final product if the plasmid DNA was not sufficiently degraded by DNases, which are meant to cut large DNA pieces into smaller ones. (theepochtimes.com)
We applied an assay that measures the stability of maintenance of an episomal plasmid in human tissue culture cells to screen for new DNA replication factors. (aacrjournals.org)

Our current research now focuses on two basic, interrelated questions: (1) How do mammalian cells decide where and when to initiate DNA replication? (nih.gov)

Thu GO, Hem LY, Hansen S, Møller B, Norstein J, Nøkleby H, Grotmol T. Is there an association between SV40 contaminated polio vaccine and lymphoproliferative disorders? (cdc.gov)

7] That same year, the pharmaceutical company, Merck, Sharp & Dohme, passed the strain (now called LS-c, 2ab/KP2) through a rhesus monkey kidney cell culture. (ronpaulforums.com)
Kidney cells from rhesus monkeys were chosen because they were found to be an effective growth medium. (ronpaulforums.com)
There was a problem, however, with using these monkey kidney cells to both create the original vaccine strains and grow the vaccine in large quantities. (ronpaulforums.com)

Cells were selected on the basis of increased murine cystic fibrosis transmembrane conductance regulator (mCFTR) mRNA content and were dilution cloned to produce distinct immortalized epithelial cell lines. (elsevierpure.com)
MLE-13a3 cell lines expressing high levels of mCFTR mRNA also expressed apolipoprotein J (apoJ) mRNA, a developmentally regulated glycoprotein expressed preferentially in fetal lung. (elsevierpure.com)
SP-A, -B, and -C were not detected or were present at low levels in the MLE cells that contained abundant CFTR and apoJ mRNA. (elsevierpure.com)
Tumor necrosis factor-α stimulated mCFTR mRNA, whereas dexamethasone, retinoic acid, and phorbol ester had no effect on the levels of mCFTR mRNA in MLE-13a3 cells. (elsevierpure.com)

Dr. Buckhaults said on social media the SV40 enhancer sequence taken from SV40 is a "standard bit of molecular biology engineering to achieve high level expression of the Neo resistance marker" and that it's been "used for decades. (theepochtimes.com)

We find that the DDX5 locus is frequently amplified in breast cancer and that breast cancer-derived cells with amplification of DDX5 are much more sensitive to its depletion than breast cancer cells and a breast epithelial cell line that lacks DDX5 amplification. (aacrjournals.org)

Researchers at Johns Hopkins have established a human cell-based system for studying sickle cell anemia by reprogramming somatic cells to an embryonic stem cell like state. (hopkinsmedicine.org)

Without large T, cells form embryonic stem cell-like clusters in three to four weeks. (hopkinsmedicine.org)
With large T, the cells started looking like embryonic stem cells in just 12 to 14 days. (hopkinsmedicine.org)

Is there a role for SV40 in human cancer? (cdc.gov)
We report an anticancer effect and mechanism of GL against human prostate cancer cells. (aspetjournals.org)
Treatment with GL significantly inhibited the viability of human prostate cancer cell line LNCaP (androgen-dependent) and its androgen-independent variant (C81) with an IC 50 of ∼1 μM (24-h treatment), at pharmacologically relevant concentrations standardized to its major active constituent z-guggulsterone. (aspetjournals.org)
Understanding how DNA replication is regulated in human cells can provide insight into cancer development and may reveal vulnerabilities that can be exploited therapeutically. (aacrjournals.org)
Having established a faster, more efficient method, the team then reprogrammed human cells that contain the mutation associated with sickle cell anemia. (hopkinsmedicine.org)
Embryonic stem cell-like clusters were visible 14 days after they initiated reprogramming and from these clusters the researchers established three different cell lines that both look and behave like human embryonic stem cells. (hopkinsmedicine.org)
We also find that expression of CK1ε is able to promote oncogenic transformation of human cells in a β-catenin-dependent manner. (plos.org)

The present invention relates generally to improved methods and pharmaceutical compositions for mobilizing hematopoietic stem and progenitor cell from bone marrow into peripheral blood by administration of at least one inhibitor of a GTPase, such as Rac1 and/or Rac2 GTPase. (justia.com)
The various mature blood cell types are all ultimately derived from a single class of progenitor cell known as hematopoietic stem cells. (justia.com)

In 1907 the zoologist Ross Granville Harrison demonstrated the growth of frog embryonic cells that would give rise to nerve cells in a medium of clotted lymph . (wikipedia.org)

We demonstrate that the transformation of breast cancer cells is dependent on PVRL4. (elifesciences.org)

The article claims SV40 also has been linked to certain cancers in humans. (politifact.com)

It is interesting to note that a representative normal prostate epithelial cell line (PrEC) was relatively more resistant to GL-mediated cellular responses compared with prostate cancer cells. (aspetjournals.org)

This causes the cells to leave G1 phase and enter into S phase, which promotes DNA replication. (wikipedia.org)
PVRL4 promotes anchorage-independence by driving cell-to-cell attachment and matrix-independent integrin β4/SHP-2/c-Src activation. (elifesciences.org)

The induction of proapoptotic Bcl-2 family proteins Bax and Bak and a decrease of antiapoptotic Bcl-2 protein Bcl-2 were observed in GL-treated cells. (aspetjournals.org)

Because of research done with SV40 in animal models, there has been some concern that the virus could cause cancer in humans. (cdc.gov)
However, most studies looking at the relationship between SV40 and cancers are reassuring, finding no causal association between receipt of SV40-contaminated polio vaccine and development of cancer. (cdc.gov)
In cases where there is nodular peritoneal thickening, ovarian cancer, and ascites, the source of ascites can be the result of various factors, including the presence of cancer cells in the peritoneal cavity. (researchgate.net)
1. Peritoneal involvement: Cancer cells can spread to the peritoneum, leading to inflammation and the production of fluid. (researchgate.net)
During all stages of tumor progression, cancer cells are subjected to inappropriate extracellular matrix environments and must undergo adaptive changes in order to evade growth constraints associated with the loss of matrix attachment. (elifesciences.org)
In fact, amplification of genes by over replication of certain regions of DNA is one of the primary mechanisms by which cancer cells become resistant to drug therapy. (nih.gov)
To uncover genetic dependencies in breast cancer cells that harbor active β-catenin signaling, we performed RNAi-based loss-of-function screens in breast cancer cell lines in which we had characterized β-catenin activity. (plos.org)
Furthermore, we demonstrate that breast cancer cells that harbor activated β-catenin activity exhibit enhanced sensitivity to pharmacological blockade of Wnt/β-catenin signaling. (plos.org)
Lung cancer is divided into two major groups: non small cell lung cancer (NSCLC) and small cell lung cancer (SCLC). (scielo.br)
2004) Akt phosphorylation and gefitinib efficacy in patients with advanced non-small-cell lung cancer. (scielo.br)
2004) Activating mutations in the epidermal growth factor receptor underlying responsiveness of non-small-cell lung cancer to gefitinib. (scielo.br)
Therefore, hematopoietic stem cells mobilized into a subject's peripheral blood by the method of the invention is useful as a source of donor cells in bone marrow transplantation for the treatment of a variety of disorders, including cancer, anemia, autoimmunity and immunodeficiency. (justia.com)

In contrast, MLE cells, cloned on the basis of abundant surfactant protein mRNAs, expressed apoJ and mCFTR mRNAs at low levels. (elsevierpure.com)
also showed that interactions between the PVRL4 in one cell and a related protein called PVRL1 in a neighboring cell were responsible for holding the cells together in clusters. (elifesciences.org)
Bac-to-Bac™ Baculovirus Expression is an efficient method for producing baculovirus for high-level protein expression in insect cells. (fishersci.com)

African green monkeys were used in early research on the SV40 virus. (politifact.com)

In mammals, functional analysis of the individual RAD51 paralogues in cell lines has shown similar but non-redundant contributions in DNA repair processes such as HR efficiency, RAD51 nuclear focus formation, sensitization to mitomycin C (MMC) and protection of perturbed replications forks [ 11 ]. (nature.com)

Improved methods and pharmaceutical compositions are provided herein for mobilizing hematopoietic progenitor cells from bone marrow into peripheral blood, comprising the administration of an effective amount of an inhibitor of GTPases, such as Rac1 and Rac2 alone or in combination. (justia.com)
Stem cells represent a very small percentage of bone marrow cells, and are normally quiescent. (justia.com)
These therapies decrease a patient's white blood cell counts, suppress bone marrow hematopoietic activity, and increase the patient's risk of infection and/or hemorrhage. (justia.com)
Current treatments to manage the problems that result from prolonged bone marrow suppression include the reinfusion of a patient's own previously harvested hematopoietic stem and progenitor cells. (justia.com)
In such procedures, patients undergo successive treatments with cell mobilization agents to cause mobilization of hematopoietic progenitor cells from the bone marrow to the peripheral circulation for harvesting. (justia.com)
After harvesting, the patient is given high dose chemotherapy or radiotherapy and the bone marrow function is reconstituted by infusion of the cells harvested earlier. (justia.com)
The use of high-dosage chemotherapy or radiotherapy for bone marrow ablation requires subsequent incorporation of hematopoietic stem cells into the patient, in which case prior harvesting of such cells is required. (justia.com)
The success of treatment crucially depends on the mobilization of the bone marrow stem cells, the subsequent return of which permits the patient to achieve reconstitution of a functioning hematopoietic system. (justia.com)

Transcription occurs from a nonintegrated, circular minichromosome in the nucleus of the plant cell, and virion DNA is synthesised in the cytoplasm by reverse transcription of the 35S RNA transcript (4, 5). (i-sis.org.uk)

Cell culture or tissue culture is the process by which cells are grown under controlled conditions, generally outside of their natural environment. (wikipedia.org)

One challenge to studying blood diseases like sickle cell anemia is that blood stem cells can't be kept alive for very long in the lab, so researchers need to keep returning to patients for more cells to study," says Cheng. (hopkinsmedicine.org)

J. Cell Biol. (cshlpress.com)
Mol Cell Biol 26:9338-9351. (scielo.br)

Bac-to-Bac™'s pFastBac™ vectors, however, recombines with the parent bacmid in DH10Bac™ E. coli competent cells to form an expression bacmid. (fishersci.com)

Cell culture techniques were advanced significantly in the 1940s and 1950s to support research in virology . (wikipedia.org)
The research team first sought to improve previously established methods for reprogramming of adult cells into so-called induced pluripotent stem (iPS) cells, which look and behave similarly to embryonic stem cells and can differentiate into many different cell types. (hopkinsmedicine.org)
The research was funded by the National Institutes of Health and the Johns Hopkins Institute for Cell Engineering. (hopkinsmedicine.org)

Publishing online in Stem Cells on May 29, the team describes a faster and more efficient method of reprogramming cells that might speed the development of stem cell therapies. (hopkinsmedicine.org)

Not only did T speed up reprogramming, we also found that it increases the total number of reprogrammed cells, which is great because often in reprogramming, not all cells go all the way," says Cheng, who explains that rigorous follow-up tests are required to determine if the reprogrammed cells really behave like pluripotent embryonic stem cells. (hopkinsmedicine.org)
True stem cells are both pluripotent-that is they can give rise to all cell types-and capable of self-renewal. (justia.com)

Anatomy31

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