• Dulbecco's Phosphate Buffered Saline (DPBS) is a balanced salt solution (BSS) used for the handling and culturing of mammalian cells. (sigmaaldrich.com)
  • Phosphate buffering maintains the pH in the physiological range. (sigmaaldrich.com)
  • Ethylene diamine tetraacetic acid (anticoagulant) and ammonium hydrogen phosphate buffer (matrix modifier) were added to blood samples prior to analysis. (cdc.gov)
  • Ultrafine and fine carbon black and titanium dioxide were suspended in phosphate buffered saline (PBS), rat and mouse bronchoalveolar lavage fluid (BALF), and PBS containing dipalmitoyl phosphatidylcholine (DPPC) and/or mouse serum albumin. (cdc.gov)
  • In an antiglobulin crossmatch performed using the column agglutination system, the donor RBCs, suspended in a hypotonic buffered saline solution, are mixed with the recipient's serum or plasma to allow antigen-antibody interaction in the upper chamber of the microtube. (medscape.com)
  • DPBS buffer is used to irrigate, wash, and dilute mammalian cells while maintaining osmotic balance. (sigmaaldrich.com)
  • Store stock solution at -80°C and dilute 1:10 with suspending buffer prior to use. (researchsquare.com)
  • You probably could, but you'd have to use sterile technique and you would dilute the preservatives and buffers so all bets are off on the outcome. (optometryforums.com)
  • Sodium Silicate in Detergents is used for removing fat particles and keeping them suspended to prevent corrosion. (nafissilicate.com)
  • the steroid is not completely dissolved in the buffer but instead is suspended in the solution in small particles. (optometryforums.com)
  • Thus, experiments were conducted to determine the best method to suspend nanosized particles. (cdc.gov)
  • During the separation, the cell only needs to be suspended in a buffer solution and enter a centrifuge, the whole processes does not involve any chemical (e.g. staining) and physical (e.g. attachment of antibody, lyses of cell membrane) effect on the cells, so the cell will remain unchanged before and after the separation. (wikipedia.org)
  • Resuspend Lipid: Oligomer Complex in Aqueous Buffer and Sonicate to Disperse. (sigmaaldrich.com)
  • 6. To fix the cells, gently resuspend the cell pellet in residual volume of wash buffer and then add 2 ml of 1x Human FoxP3 Buffer A. (bdbiosciences.com)
  • Suspend the pellet obtained in the previous centrifugation in 1 ml of PBS-EDTA. (cdc.gov)
  • This can be accomplished by dissolving the lipid in an organic solvent and either dispersing the organic in an aqueous solution of the DNA or incubating the lipid and DNA together in the organic solvent. (sigmaaldrich.com)
  • Adjust the concentration such that the volume of ethanol lipid solution added to the aqueous oligomer solution is 10% of the aqueous solution volume. (sigmaaldrich.com)
  • The key concept is that larger cells tend to stay within the flowing buffer solution while smaller cells will be washed away follow the buffer solution (different sedimentation property within the buffer solution), and cells will have different sedimentation properties in different cell cycle stages. (wikipedia.org)
  • Then when the flow rate of the buffer solution increases, the solution tends to push the cells towards the middle of the CCE. (wikipedia.org)
  • The CCE is also limited to cells which are able to be individually suspended in the buffer solution. (wikipedia.org)
  • Briefly, a suspension containing a mixture of 2×10 6 lymphocytes and 2×10 6 of 1301 cells was denatured at 82°C for 10 min, with and without the fluorescein-conjugated PNA telomere probe in the hybridisation solution. (ersjournals.com)
  • Dulbecco′s PBS without these ions can be used to wash and rinse suspended cells. (sigmaaldrich.com)
  • Blood cells are poorconductors of electricity but are suspended in an electrically conductivediluent. (slideshare.net)
  • Suspend the cells with BD Pharmingen™ Stain Buffer (FBS)* to ten million cells/ml. (bdbiosciences.com)
  • Due to its expertise in high-performance single item picking, the company chose KNAPP to design and realise a future-oriented automated logistics solution. (logisticsbusiness.com)
  • The ISE is calibrated using fluoride solutions with known concentrations, such as calibrators. (cdc.gov)
  • creating a suspension is a little tricky because if the buffer conditions are not correct the steroid will precipitate out in the bottom of the bottle. (optometryforums.com)
  • Both calibrators and water samples are diluted with a total ionic strength adjustment buffer (TISAB) and measured under the same conditions. (cdc.gov)
  • Two specimens of the same material were suspended by a platinum wire inside weighing bottles containing 50 mL of water or a lactate buffer solution (pH=2.74) prepared according to ISO 9917. (bvsalud.org)
  • Simultaneously, a clean weighing bottle with either lactate buffer or distilled water was used as a blank estimation for each specimen pair. (bvsalud.org)
  • 2 chambers filled with a conductive buffered electrolyte solution separated by a glass tube having a small aperture. (slideshare.net)
  • The normal procedure for preparation of lipid: DNA complex requires suspending the cationic lipid in aqueous buffer and sonication to form small vesicles. (sigmaaldrich.com)
  • The NHANES program suspended field operations in March 2020 due to the coronavirus disease 2019 (COVID-19) pandemic. (cdc.gov)
  • TMA), phthalic anhydride, tetrachloride techniques (i.e. intradermal injection with OAA suspended phthalic anhydride and hexahydrophthalic anhydride, in plant oil or liquid paraffin) [6, 9, 10]. (cdc.gov)
  • 2. Fill tube with Tris-EDTA buffer (formula) and mix vigorously. (who.int)
  • However, BALF was an excellent media in which to suspend nanoparticles. (cdc.gov)
  • Therefore, buffer flow rate can be used to control size sorting within the elutriation chamber. (wikipedia.org)
  • Polysciences offers a broad range of products and tailored solutions in addition to custom synthesis and contract manufacturing services for the life sciences, bioprocessing, microparticles, medical device, specialty chemical and electronic chemical industries. (polysciences.com)
  • Prepared human blood sample was introduced into the atomizer chamber as an aerosol, formed by nebulization of the sample solution (Nakashima et al. (cdc.gov)