• CD47 (Cluster of Differentiation 47) also known as integrin associated protein (IAP) is a transmembrane protein that in humans is encoded by the CD47 gene. (wikipedia.org)
  • CD47 ( C luster of D ifferentiation 47) also known as integrin associated protein (IAP) is a transmembrane protein that in humans is encoded by the CD47 gene . (wikidoc.org)
  • CD47 also known as Rh-associated protein, gp42, integrin-associated protein (IAP), and neurophilin, is a 42-52 kD member of the immunoglobulin superfamily containing a five-pass transmembrane attachment. (biolegend.com)
  • The glycoprotein CD47 (integrin-associated protein, IAP) is present on the surface of virtually all cells, including red blood cells (RBCs). (ashpublications.org)
  • Additionally, CD47 blocking antibodies inhibit proliferation of unstimulated astrocytoma cells but not normal astrocytes. (wikipedia.org)
  • However, apoptosis was not observed following culture with other anti-CD47 antibodies. (wikipedia.org)
  • In a recent study, researchers from the Stanford University School of Medicine (USA) found that cells with surface antigen CD47 are extremely abundant in -atherosclerotic plaque, and that CD47-blocking antibodies reverse defects in efferocytosis, normalize the clearance of diseased vascular tissue and ameliorate atherosclerosis in multiple mouse models. (edu.pl)
  • Disrupting the binding of CD47 to SIRPα has emerged as a promising immunotherapeutic strategy for advanced cancers by potentiating antibody-dependent cellular phagocytosis (ADCP) of targeted antibodies. (aacrjournals.org)
  • Murine SIRPα-Fc-CD40L outperformed CD47 blocking and CD40 agonist antibodies in murine CT26 tumor models and synergized with immune checkpoint blockade of PD-1 and CTLA4. (aacrjournals.org)
  • IL-2 and IFN-γ), immune checkpoint blockade (ICB) therapy (e.g., anti-PD-1/PD-L1 antibodies), and adoptive T-cell transfer (e.g., chimeric antigen receptor (CAR) T-cell therapy) [ 3 - 5 ]. (thno.org)
  • We now offer our popular anti-flavivirus and Zika antibodies, including Anti-Flavivirus group antigen , Anti-Envelope protein , and Anti-NS1 , in the new ferret versions. (absoluteantibody.com)
  • Similarly, CD47 −/− C57BL/6 mice were much more sensitive than their wild-type counterparts to experimental passive AIHA induced by anti-RBC monoclonal antibodies. (ashpublications.org)
  • CD47 is a 50 kDa membrane receptor that has extracellular N-terminal IgV domain, five transmembrane domains, and a short C-terminal intracellular tail. (wikipedia.org)
  • CD47 is a high affinity receptor for thrombospondin-1 (TSP-1), a secreted glycoprotein that plays a role in vascular development and angiogenesis, and in this later capacity the TSP1-CD47 interaction inhibits nitric oxide signaling at multiple levels in vascular cells. (wikipedia.org)
  • CD47 interacts with signal-regulatory protein alpha (SIRPα), an inhibitory transmembrane receptor present on myeloid cells. (wikipedia.org)
  • Probable immunoglobulin-like cell surface receptor. (nih.gov)
  • CD47 has a role in both cell adhesion by acting as an adhesion receptor for THBS1 on platelets, and in the modulation of integrins and plays an important role in memory formation and synaptic plasticity in the hippocampus by similarity. (abeomics.com)
  • CD47 is the receptor for SIRPA, binding to which prevents maturation of immature dendritic cells and inhibits cytokine production by mature dendritic cells. (abeomics.com)
  • CD47 functions as an adhesion molecule and thrombospondin receptor and is non-covalently associated with β3 integrins CD51/CD61, CD41/CD61. (biolegend.com)
  • Breast cancer (BC)-initiating cells typically express high levels of CD44, a surface receptor for the extracellular matrix protein Hyaluronan, low levels of CD24, and exhibit high aldehyde dehydrogenase (ALDH) activity ( 3 ). (life-science-alliance.org)
  • Lipopolysaccharide, in addition, induced co-clustering with Toll-like receptor 4, Fcgamma-RIIIa (CD16a) and the tetraspanin CD81 while CD47 was dissociated. (unideb.hu)
  • SIRPα-Fc-CD40L bound CD47 and CD40 with high affinity and activated CD40 signaling in the absence of Fc receptor cross-linking. (aacrjournals.org)
  • BIT, an immune antigen receptor-like molecule in the brain. (medecinesciences.org)
  • The CD4 (T4) antigen is an essential component of the receptor for the AIDS retrovirus. (medecinesciences.org)
  • Adoptive T cell therapy (ACT) with tumor infiltrating lymphocytes (TIL), T cell receptor (TCR) and Chimeric Antigen Receptor (CAR) engineered T cells, or hematopoietic stem cell transplantation, is a promising new approach to cancer treatment. (nih.gov)
  • High expression of CD47, a cell surface receptor on several types of cancer cells, has been identified as a 'don't eat me signal' that inhibits their killing by macrophages or NK cells. (nih.gov)
  • Here, using dual-color direct stochastic optical reconstruction microscopy, we report that Fcγ receptor I (FcγRI), FcγRII, and SIRPα are not homogeneously distributed at macrophage surfaces but are organized in discrete nanoclusters, with a mean radius of 71 ± 11 nm, 60 ± 6 nm, and 48 ± 3 nm, respectively. (ox.ac.uk)
  • SHPS1 Immunoglobulin-like cell surface receptor for CD47. (affbiotech.com)
  • CD47 acts like a marker of self by ligating the macrophage inhibitory receptor signal regulatory protein α (SIRPα). (ashpublications.org)
  • Treatment with a disulfide-linked antibody dimer induces apoptosis of CD47-positive primary B-CLL and leukemic cells (MOLT-4 and JOK-1). (wikipedia.org)
  • Immunotherapies with antibody-drug-conjugates (ADC) and CAR-T cells, targeted at tumor surface antigens (surfaceome), currently revolutionize clinical oncology. (lu.se)
  • The efficacy of a therapeutic antibody depends on the Fab fragment and its binding activity to the target antigen, but also depends on the Fc fragment and its interaction with key Fc receptors.Therefore, candidates must be tested against a panel of receptors during antibody engineering. (acrobiosystems.com)
  • The CC2C6 antibody recognizes human CD47 and has been shown to be useful for flow cytometry. (biolegend.com)
  • The CC2C6 monoclonal antibody can block the binding of HCD47 antibody to CD47. (biolegend.com)
  • In several different atherosclerotic model mice, blocking CD47 with anti-CD47 antibody significantly reduced accumulation of arterial plaque. (edu.pl)
  • An atherosclerotic-model antibody, the anti-CD47 antibody, was sorbed onto the particle surfaces in water and phosphate-buffered saline (PBS) for 4 h. (edu.pl)
  • 1 However, how to use CD47 antibody to achieve effective drug delivery remains a key issue to be solved in future research. (edu.pl)
  • Bryan Hancock, PhD (Fate Therapeutics) previewed the preclinical studies supporting the investigational new drug application for FT536, a CAR NK cell product derived from an inducible pluripotent stem-cell line engineered for enhanced effector cell functionality, persistence, and multi-antigen targeting capabilities through CD16-mediated antibody dependent cellular cytotoxicity (ADCC). (sitcancer.org)
  • The mouse monoclonal antibody 5E10 recognizes CD90/Thy-1, a GPI-anchored cell surface glycoprotein expressed predominantly on thymocytes, hematopoietic stem cells and neurons. (exbio.cz)
  • Conversely, the CD47 antibody B6H12 that blocks SIRPα binding enhances macrophage-dependent clearance of tumors in several mouse models, although others have shown that such clearance can be independent of SIRPα signaling. (nih.gov)
  • Magrolimab is a first-in-class monoclonal antibody that targets and inhibits CD47, a "don't eat me" signal overexpressed on the surface of cancer cells that allows them to evade the innate immune response. (bjh.be)
  • The CD47/SIRPα interaction leads to bidirectional signaling, resulting in different cell-to-cell responses including inhibition of phagocytosis, stimulation of cell-cell fusion, and T-cell activation. (wikipedia.org)
  • Notably, CXCR4/CXCL12 cross-signal via the T and B cell receptors (TCR and BCR) and co-internalize with CD47, promoting tumor cell phagocytosis by macrophages in an anti-tumor immune process called ImmunoGenic Surrender (IGS). (unisr.it)
  • These data illustrated that whereas CD47/SIRPα inhibition could potentiate tumor cell phagocytosis, CD40-mediated activation of a type I interferon response provided a bridge between macrophage- and T-cell-mediated immunity that significantly enhanced durable tumor control and rejection. (aacrjournals.org)
  • When tested intravenously in monkeys, LVs with high levels of CD47 were more resistant to phagocytosis and were less susceptible to detection by antigen-presenting cells than normal LVs were, the researchers reported. (international-biopharma.com)
  • Blocking CD47 leads to increased recognition and phagocytosis of cancer cells. (bjh.be)
  • CD47 belongs to the immunoglobulin superfamily and partners with membrane integrins and also binds the ligands thrombospondin-1 (TSP-1) and signal-regulatory protein alpha (SIRPα). (wikipedia.org)
  • The CD47/SIRPα pathway is an endogenous pathway that facilitates clearance of aged or dying cells ( 1 ). (aacrjournals.org)
  • The antiphagocytic activity of CD47/SIRPα serves as a macrophage checkpoint or "do not eat me" signal ( 1 ). (aacrjournals.org)
  • Co-ligation of SIRPα with CD47 abrogates nanocluster segregation. (ox.ac.uk)
  • On binding with CD47, mediates cell-cell adhesion. (nih.gov)
  • CD47 Interaction with SIRPG mediates cell-cell adhesion, enhances superantigen-dependent T-cell-mediated proliferation and costimulates T-cell activation. (abeomics.com)
  • Two of the proteins (ROR2 and CD47) involved in this study are expressed on the surface of LMS cells. (sarcomahelp.org)
  • 3' UTR-dependent protein localization has the potential to be a widespread trafficking mechanism for membrane proteins because HuR binds to thousands of mRNAs, and we show that the long 3' UTRs of CD44, ITGA1 and TNFRSF13C, which are bound by HuR, increase surface protein expression compared to their corresponding short 3' UTRs. (nih.gov)
  • We provide a quantitative ranking of more than 600 surface resident and endocytosed proteins, and their regulation by hypoxia, serving as a resource to the cancer research community. (lu.se)
  • Other cell surface proteins, including CD326 (EpCAM), epithelial specific antigen, CD133, CD166, CD47, CD201, and ABCG2 have been reported as CSC markers for different BC subtypes and drug-resistant tumors ( 4 , 5 ). (life-science-alliance.org)
  • Additionally, BMVs consist of considerable proteins, for example, original bacterial antigens and pathogen-associated molecular pattern components [ 5 ] . (encyclopedia.pub)
  • They have previously shown in an LMS mouse model that inhibiting the CD47 protein allows immune cells called macrophages to more effectively destroy LMS cells. (sarcomahelp.org)
  • Following the co-internalization of CXCR4 and CD47 in tumor cells, macrophages phagocytose them and cross-present their antigens to the adaptive immune system, leading to tumor rejection in a fraction of mice. (unisr.it)
  • Saginario C, Qian HY, Vignery A. Identification of an inducible surface molecule specific to fusing macrophages. (medecinesciences.org)
  • The CD47 protein is known to inhibit this destructive process, so San Raffaele Telethon Institute for Gene Therapy scientific director Luigi Naldini and colleagues hypothesized that attaching CD47 to LV could help gene therapies escape macrophages. (international-biopharma.com)
  • Thus, a nanometer- and micron-scale reorganization of activating and inhibitory receptors occurs at the surface of human macrophages concurrent with signal integration. (ox.ac.uk)
  • BST2 bone marrow stromal cell antigen 2 (Tetherin): Candida albicans enhanced the production of the CCR5-interacting chemokines CCL3, CCL4, and CCL5, and stimulates the production of interferon-a and the restriction factors APOBEC3G, APOBEC3F, and tetherin (BST2) in macrophages Rodriguez et al, 2013 . (polygenicpathways.co.uk)
  • Second, T cell therapies using Tregs (either polyclonal, antigen-specific, or genetically engineered to express chimeric antigen receptors) to establish active dominant immune tolerance or T cells (engineered to express chimeric antigen receptors) to delete pathogenic immune cells. (frontiersin.org)
  • Membrane vesicles fuse with the target cell membrane, or they bind to the receptors on the cell surface, to transfer special effects. (encyclopedia.pub)
  • In resting cells, CD14 was associated with CD55, the Fcgamma-receptors CD32 and CD64 and the pentaspan CD47. (unideb.hu)
  • Cell surface receptors containing an immunoglobin domain. (embl.de)
  • In this manner mild reactivity of wild-type RBCs with macrophage phagocytic receptors is tolerated, whereas otherwise identical CD47-deficient RBCs are rapidly eliminated. (ashpublications.org)
  • In mice bearing brain metastatic 4T1br3 tumors, anti-CD47 treatment shrank tumors by roughly 50% compared to controls, which accompanied by a 5-fold increase in expression of F4/80 macrophage markers in the tumors. (sitcancer.org)
  • Vignery A. Macrophage multinucleation is accompanied by the expression of new soluble and membrane antigens in mice. (medecinesciences.org)
  • CD47, a ligand for MFR, participates in macrophage multinucleation. (medecinesciences.org)
  • As proof-of-concept, the established target antigen CD44 was identified as a commonly and abundantly expressed surface protein with high endocytic activity. (lu.se)
  • CD47 is a cell surface glycoprotein implicated in multiple cellular functions including recognition of self. (researchgate.net)
  • One such mechanism involves the upregulation of immune checkpoints, such as programmed death-1 (PD-1)/programmed death-ligand 1 (PD-L1), cluster of differentiation 86 (CD86)/cytotoxic T-lymphocyte antigen 4 (CTLA-4) [ 16 ]. (biomedcentral.com)
  • Over the past decades, André Veillette's team has identified and characterized numerous cell surface and intracellular molecules that play a crucial role in normal cell physiology. (ircm.qc.ca)
  • However, these isoforms are highly conserved between mouse and man, suggesting an important role for the cytoplasmic domains in CD47 function. (wikipedia.org)
  • This facilitates interaction of SET with the newly translated cytoplasmic domains of CD47 and results in subsequent translocation of CD47 to the plasma membrane via activated RAC1 (ref. 5). (nih.gov)
  • The activation of CD47 induces rapid apoptosis of T cells. (wikipedia.org)
  • Similarly, CD47 ligation rapidly induces apoptosis in B-cell chronic lymphocytic leukemia (CLL) cells. (wikipedia.org)
  • Loss of CD47 allows sustained proliferation of primary murine endothelial cells and enables these cells to spontaneously reprogram to form multipotent embryoid body-like clusters. (wikipedia.org)
  • Expression of several stem cell markers, including c-Myc, is elevated in CD47-null endothelial cells and a human T cell line lacking CD47. (wikipedia.org)
  • CD47 is ubiquitously expressed in human cells and has been found to be overexpressed in many different tumor cells. (wikipedia.org)
  • Activation of CD47 with TSP-1 increases proliferation of human U-87 MG and U373 astrocytoma cells but not normal astrocytes. (wikipedia.org)
  • Though the exact mechanism is unclear, it is likely that CD47 promotes proliferation via the PI3K/Akt pathway in cancerous cells but not normal cells. (wikipedia.org)
  • Activation of CD47 with TSP-1 in wild-type cells inhibits proliferation and reduces expression of stem cell transcription factors. (wikipedia.org)
  • Targeted' therapy starts with the discovery of a protein that is expressed on the surface of tumor cells but not (or in a limited way) in normal human tissues. (sarcomahelp.org)
  • The third protein involved in the study, CSF1 , is not expressed on the cell surface but instead is secreted by the tumor cells into the surrounding environment. (sarcomahelp.org)
  • Engagement on T-cells by CD47 on antigen-presenting cells results in enhanced antigen-specific T-cell proliferation and costimulates T-cell activation. (nih.gov)
  • In addition, CXCL12 and CXCR4 are involved in antigen recognition by T and B cells and in shaping the tumor microenvironment (TME), mainly towards dampening immune responses. (unisr.it)
  • Persistent increases in serum levels of interferon gamma and granzyme B were observed in both Arms (6/20 patients in Arm 4 and 2/20 patients in Arm B). These data suggest that the generation of tumor-antigen-specific T cells through vaccination may help overcome primary resistance to anti-PD-1 monotherapy in prostate cancer. (sitcancer.org)
  • Flow cytometry analysis (surface staining) of CD90 in Jurkat cells and SP2 cells with anti-CD90 (5E10) PE-Cy5. (exbio.cz)
  • Signal-regulatory protein alpha (SIRPalpha) but not SIRPbeta is involved in T-cell activation, binds to CD47 with high affinity, and is expressed on immature CD34(+)CD38(-) hematopoietic cells. (exbio.cz)
  • Role of CD47 as a marker of self on red blood cells. (medecinesciences.org)
  • bsAbs have two different antigen binding sites, which enables them to simultaneously target tumour cells and immune effector cells (T-cells). (bjh.be)
  • Dendritic cells (DCs) are professional antigen-presenting cells that have multiple subpopulations with different phenotypes and immune functions. (biomedcentral.com)
  • Finally, mononuclear cells isolated from lung (L-MCs), which are used as precursors for L-DCs, expressed more antigen-presenting cell-associated markers such as MHC class II and CD172α compared to their counterparts from blood. (biomedcentral.com)
  • in the absence of CD47 mice show defects in host defense and β3 integrin-dependent ligand binding, migration, and cellular activation. (biolegend.com)
  • In the absence of CD47, mice show defects in host defense and beta 3 integrin-dependent ligand binding, migration, and cellular activation. (biolegend.com)
  • Knockout of CD47 led to 60% increased survival and 89% decreased metastatic brain lesions in 4T1-bearing mice compared to controls. (sitcancer.org)
  • We show here that virtually all CD47-deficient nonobese diabetic (NOD) mice spontaneously develop severe lethal autoimmune hemolytic anemia (AIHA) at 180 to 280 days of age, whereas none of the control CD47 + NOD mice develop lethal AIHA at least during the first year of life. (ashpublications.org)
  • The "don't eat me signal" CD47 is a pleitropic surface marker expressed on numerous cell types that inhibits myeloid cell phagocytic activity through binding SIRPalpha as well as an antagonizes T cell function mediated by interaction with matricellular protein Thrombospondin-1. (sitcancer.org)
  • CD47 is very broadly distributed on normal adult tissues. (dimabio.com)
  • More recent studies suggested that DCs derived from tissues without "danger" signal stimulation should be regarded as immature DCs, based on their major role in antigen uptake and endocytosis of antigens [ 11 , 14 ]. (biomedcentral.com)
  • The apoptosis inducing function of CD47 appears to be dependent on activation of specific epitopes on the extracellular domain. (wikipedia.org)
  • A total of 318 differentially expressed genes were associated with anti-CD47 treatment, with enrichment for reduced signatures of extracellular matrix remodeling and upregluation of pathways involved in tertiary lymphoid structure formation. (sitcancer.org)
  • The researchers integrated the protein CD47 into the surface of lentiviral vectors (LVs) so they could escape detection and destruction by the immune system. (international-biopharma.com)
  • Antigens, CD47" is a descriptor in the National Library of Medicine's controlled vocabulary thesaurus, MeSH (Medical Subject Headings) . (rush.edu)
  • These interactions result in CD47/integrin complexes that affect a range of cell functions including adhesion, spreading and migration. (wikipedia.org)
  • Yu J, Ruan Q, Nie X. Synthesis and characterization of atherosclerotic target anti-CD47 functionalized by nano- polyelectrolyte complexes between chitosan and hyaluronic acid and in vivo and in vitro targeting experiments. (edu.pl)
  • CD47 interacts with several membrane integrins, most commonly integrin αVβ3. (wikipedia.org)
  • CD47 interacts with several membrane integrins, most commonly integrin avb3. (wikidoc.org)
  • New data indicate that CXCR4 interacts with the surface protein CD47 in a novel form of immunosurveillance, called ImmunoGenic Surrender (IGS). (unisr.it)
  • Binding of TSP-1 to CD47 influences several fundamental cellular functions including cell migration and adhesion, cell proliferation or apoptosis, and plays a role in the regulation of angiogenesis and inflammation. (wikipedia.org)
  • Jessica D. Mackert, PhD (Wake Forest University) described preclinical data supporting CD47 as a target for the treatment of brain metastases in triple-negative breast cancer (TNBC). (sitcancer.org)
  • This preclinical data supports CD47 as a potential target for the immunotherapeutic treatment of brain metastases. (sitcancer.org)
  • In our model of 3' UTR-dependent protein localization, the long 3' UTR of CD47 acts as a scaffold to recruit a protein complex containing the RNA-binding protein HuR (also known as ELAVL1) and SET to the site of translation. (nih.gov)
  • As one example, the respiratory tract represents one of the largest surface areas in the body and acts as an interface with the external environment that is frequently exposed to foreign particles or pathogens. (biomedcentral.com)
  • There are still significant barriers to therapeutic success because of tumor-specific antigens (TA) and toxicities associated with treatment [ 19 ]. (biomedcentral.com)
  • Human peripheral blood lymphocytes were stained with anti-human CD47 (clone CC2C6) PE/Dazzle™ 594 (filled histogram) or mouse IgG1, κ PE/Dazzle™ 594 isotype control (open histogram). (biolegend.com)
  • They act as a surveillance system to detect foreign antigens and shape immunogenic or tolerogenic responses [ 1 ]. (biomedcentral.com)
  • Concurrent with this observation, L-MCs possessed significantly more antigen-uptake capacity compared to their counterparts from blood. (biomedcentral.com)
  • Our findings provide a comprehensive understanding of the surface-endocytome and its remodeling by hypoxia in GBM as a resource for exploration of targets for immunotherapeutic approaches in GBM. (lu.se)