CD70Receptor superfamilyMoleculeReceptorsOX40CD40ProteinBindsChimeric Antigen ReceptorTumor-associated antigensCD11bImmunoglobulin superfamilyCellsSoluble CD27Ligand superfamilyDifferentiation AntigensGlycoproteinCD19LymphocytesProliferationFlow cytometryRecombinantICOSHumansDisulfide-linked homodimerAntibodiesSubsetsActivationMonoclonalExpressionCostimulationViralDetects
CD706
- In contrast to the expression of other TNFR/TNF family members, expression of CD27 and its ligand CD70 is predominantly confined to lymphocytes. (thermofisher.com)
- RgpA, a cystein proteinase, although activating T cells through the protease-activated receptors (PARs), degradates CD27 and counteracts T cell activation mediated by CD27 and its ligand CD70. (thermofisher.com)
- CD27 binds to CD70, and plays a role in costimulation of T cell activation and regulation of B cell differentiation and proliferation. (biolegend.com)
- Bowman MR, Crimmins MA, Yetz-Aldape J, Kriz R, Kelleher K, Herrmann S. The cloning of CD70 and its identification as the ligand for CD27. (bdbiosciences.com)
- CD27 and its ligand CD70 are involved in multiple aspects of T cell biology, including the costimulation and expansion of CD4+ and CD8+ T cells and differentiation to memory CD8+ T cells. (stemcell.com)
- CD27 ligand (CD27L), also named CD70, is a type II transmembrane glycoprotein belonging to the TNF superfamily (TNFSF) and has been designated TNFSF7 (1, 2). (bio-techne.com)
Receptor superfamily8
- CD27 is a 50 kDa member of the tumor necrosis factor (TNF) receptor superfamily that includes CD40 and CD30. (thermofisher.com)
- Four stimulatory checkpoint molecules are members of the tumor necrosis factor (TNF) receptor superfamily-CD27, CD40, OX40, GITR and CD137. (wikipedia.org)
- It is a lymphocyte-specific member of the TNF-receptor superfamily. (biolegend.com)
- CD27, a member of the tumor necrosis factor receptor superfamily, activates NF-κB and stress-activated protein kinase/c-Jun N-terminal kinase via TRAF2, TRAF5, and NF-κB-inducing kinase. (bdbiosciences.com)
- The LG.3A10 antibody reacts with CD27, a 45 - 55 kDa member of the tumor necrosis factor receptor superfamily. (stemcell.com)
- 4-1BB (CD137/TNFSF9), a member of the tumor necrosis factor receptor superfamily (TNFR-SF), is a potent co-stimulatory receptor that plays a prominent role in augmenting effector functions of CD8+ T cells, but also CD4+ T cells and NK cells. (bvsalud.org)
- OX40 is a T cell co-stimulatory molecule of the TNF receptor superfamily that coordinates with other co-stimulators (CD28, CD40, CD30, CD27 and 4-1BB) to manage the activation of the immune response. (genscript.com)
- CD27L interacts with CD27, a member of the TNF receptor superfamily that is expressed on natural killer (NK) cells and subsets of T and B cells (2, 4, 5). (bio-techne.com)
Molecule5
- CD27: This molecule supports antigen-specific expansion of naïve T cells and is vital for the generation of T cell memory. (wikipedia.org)
- CD40: This molecule, found on a variety of immune system cells including antigen presenting cells has CD40L, otherwise known as CD154 and transiently expressed on the surface of activated CD4+ T cells, as its ligand. (wikipedia.org)
- CD146, also known as melanoma cell adhesion molecule (MCAM or Mel-CAM), MUC18, S-Endo1, and A32 antigen, is an integral membrane glycoprotein that belongs to the Ig superfamily. (angioproteomie.com)
- Several antigens have been used as targets for CAR-T cell therapy against MM, including B cell maturation antigen (BCMA), CD19, CD138, signaling lymphocytic activation molecule 7 (SLAM7), and immunoglobulin light chains. (biomedcentral.com)
- The TCR complex and CD4 each bind to distinct regions of the antigen-presenting MHCII molecule - α1/β1 and β2, respectively. (wikidoc.org)
Receptors5
- High expression levels of CD27 appear to be dependent on proper ligation of antigen receptors. (thermofisher.com)
- Introduction: Targeting costimulatory receptors of the tumor necrosis factor receptor (TNFR) superfamily with agonistic antibodies is a promising approach in cancer immuno therapy. (bvsalud.org)
- The efficacy of a therapeutic antibody depends on the Fab fragment and its binding activity to the target antigen, but also depends on the Fc fragment and its interaction with key Fc receptors.Therefore, candidates must be tested against a panel of receptors during antibody engineering. (acrobiosystems.com)
- The ability of the B cell to respond in a specific, yet sensitive manner to the various antigens is achieved with the use of low-affinity antigen receptors. (thermofisher.com)
- Like many cell surface receptors/markers, CD4 is a member of the immunoglobulin superfamily . (wikidoc.org)
OX403
- Like CD27, OX40 promotes the expansion of effector and memory T cells, however it is also noted for its ability to suppress the differentiation and activity of T-regulatory cells, and also for its regulation of cytokine production. (wikipedia.org)
- Second and third generation of CARs are composed of a combination of costimulatory domains such as immunoglobulin (Ig) superfamily members, CD28 or inducible T cell costimulatory (ICOS), and the tumor necrosis factor receptor (TNFR) superfamily members 4-1BB, OX40 and CD27. (encyclopedia.pub)
- Tumor necrosis factor ligand superfamily member 4 (TNFSF4) is also known as glycoprotein Gp34, OX40 ligand (OX40L), TAX transcriptionally-activated glycoprotein 1 and CD252, which belongs to the tumor necrosis factor family. (genscript.com)
CD401
- The CD27 antigen is a lymphocyte-specific member of the tumor necrosis factor receptor (TNFR) super family, which also includes nerve growth factor receptor, CD30, CD40, CD95 (Fas), CD120a, CD120b, CD134 (OX 40), and CD137. (bdbiosciences.com)
Protein5
- Full length human recombinant protein of human CD27 (NP_001233) produced in HEK293T cell. (thermofisher.com)
- CD27-binding protein (SIVA), a proapoptotic protein, can bind to this receptor and is thought to play an important role in the apoptosis induced by this receptor. (thermofisher.com)
- CD27 is a 50-55 kD type I membrane protein also known as S152 and T14. (biolegend.com)
- A transmembrane protein belonging to the tumor necrosis factor superfamily that specifically binds to CD27 ANTIGEN . (nih.gov)
- Chimeric antigen receptor (CAR) T cell therapy has emerged as a novel immunotherapy which modifies T cells with CAR, an artificial fusion protein that incorporates an extracellular antigen recognition domain, a transmembrane domain, and an intracellular domain including costimulation and signaling components [ 4 , 5 ]. (biomedcentral.com)
Binds2
- This Antibody was verified by Knockout to ensure that the antibody binds to the antigen stated. (thermofisher.com)
- A membrane-bound tumor necrosis family member found primarily on activated T-LYMPHOCYTES that binds specifically to CD30 ANTIGEN. (wakehealth.edu)
Chimeric Antigen Receptor2
- Chimeric Antigen Receptor (CAR) T-cells are T lymphocytes that have been specifically engineered to target malignant cells. (encyclopedia.pub)
- There is an unmet need to develop novel therapies for refractory/relapsed MM. In the past few years, chimeric antigen receptor (CAR)-modified T cell therapy for MM has shown promising efficacy in preclinical and clinical studies. (biomedcentral.com)
Tumor-associated antigens2
- Taking advantage of the diverse γδ TCR repertoire or other ligand-receptor interactions, γδ T cells can recognize a broad spectrum of tumor-associated antigens (TAAs) in a major histocompatibility complex (MHC)-independent manner, thereby activating downstream pleiotropic effects. (explorationpub.com)
- 0.05), suggesting the selective elimination of DCs cross-presenting tumor-associated antigens by iNKT cells. (biomedcentral.com)
CD11b2
- Combined with using CD27 and CD11b staining, CD146 may be an alternative marker to detect final stages of NK cell maturation and define NK cell subsets. (angioproteomie.com)
- In the mouse likewise CD27 and CD11b (Mac-1) are used to dissect NK cells into functionally and developmentally different subsets [ 9 ]. (biomedcentral.com)
Immunoglobulin superfamily2
- CEA and related genes make up the CEA family belonging to the immunoglobulin superfamily. (wikidoc.org)
- CD19 is a member of the immunoglobulin superfamily and has two Ig like domains. (thermofisher.com)
Cells35
- CD27 is expressed as a disulfide-linked homodimer on mature thymocytes, peripheral blood T cells and a subpopulation of B cells. (thermofisher.com)
- Activation of T cells via TCR-CD3 complex results in upregulation of CD27 expression on the plasma membrane as well as in the release of its soluble 28-32 kDa form, sCD27, detected in the plasma, urine or spinal fluid. (thermofisher.com)
- CD27 is also a memory marker of B cells. (wikipedia.org)
- OX40's value as a drug target primarily lies in the fact that, being transiently expressed after T-cell receptor engagement, it is only upregulated on the most recently antigen-activated T cells within inflammatory lesions. (wikipedia.org)
- The ligand for GITR is mainly expressed on antigen presenting cells. (wikipedia.org)
- CD27 is expressed on medullary thymocytes, virtually all mature T cells, some B cells, and NK cells. (biolegend.com)
- The CD27 antibody, clone L128, is derived from hybridization of mouse Sp2/0 cells with spleen cells from BALB/c mice immunized with activated peripheral blood lymphocytes. (bdbiosciences.com)
- Further evidence that the CD4+CD45RA-CD27- T cell subset contains specialized primed T cells. (bdbiosciences.com)
- Elrefaei M, El-Sheikh N, Kamal K, Cao H. HCV-specific CD27-CD28- memory T cells are depleted in hepatitis C virus and Schistosoma mansoni co-infection. (bdbiosciences.com)
- T cell subpopulation phenotypes in filarial infections: CD27 negativity defines a population greatly enriched for Th2 cells. (bdbiosciences.com)
- B-cell antigens are expressed on the immature cells that make up the tumor in virtually all cases of Burkitt lymphoma. (lookformedical.com)
- CD27 is found on peripheral T cells, memory B cells, and NK cells and in mice has been found on most thymocytes, except CD46-CD8- precursors. (stemcell.com)
- Additionally, CD27 plays important roles in T cell - B cell interactions and the maturation of effector T cells. (stemcell.com)
- The expansion of activated B cells and their differentiation to plasma cells is also reported to be enhanced by CD27. (stemcell.com)
- CARs are synthetic molecules designed to activate T cells in response to a specific antigen, mimicking T cell activation through the T cell receptor (TCR) and associated costimulatory molecules. (encyclopedia.pub)
- CAR T-cells are specifically designed to target an antigen on the surface of cells and they need to be metabolically fit to reach the tumor, survive in an immunosuppressive microenvironment and display their cytolytic function [ 12 ] . (encyclopedia.pub)
- In the presence of sub-optimal amounts of Mouse CD3e Monoclonal Antibody (Catalog # MAB484 ), Recom-binant Mouse CD27 Ligand/ TNFSF7 (Catalog # 783-CL ) stimulates proliferation in mouse splenic T cells in a dose-dependent manner (orange line). (bio-techne.com)
- Measured by its ability to neutralize CD27 Ligand/TNFSF7-induced proliferation in mouse splenic T cells. (bio-techne.com)
- CD27L cell surface expression is induced by antigen receptor activation in B cells and at low levels in mouse T cells. (bio-techne.com)
- Ligation of CD27 on T cells provides costimulatory signals that are required for T cell proliferation, clonal expansion and the promotion of effector T cell formation (2). (bio-techne.com)
- Moreover, CD27(-)IgA(+) B cells could be a marker for treatment with TNF-α blockers. (eyehospital.nl)
- BCMA, a member of the tumor necrosis factor (TNF) superfamily, is exclusively expressed in a subpopulation of B cells, normal plasma cells, and malignant plasma cells. (biomedcentral.com)
- Here we demonstrate that certain HCV(+)MC(+) subjects have clonal expansions of immunoglobulin M (IgM)(+)kappa(+)IgD(low/-)CD21(low)CD27(+) B cells. (ox.ac.uk)
- The finding that these hypermutated, marginal zone-like IgM(+)CD27(+) B cells are clonally expanded in certain subjects with MC offers insight into mechanisms of HCV-associated MC and B-cell malignancy. (ox.ac.uk)
- B19-specific T cells developed and maintained an activated CD38+ phenotype, with strong expression of perforin and CD57 and downregulation of CD28 and CD27. (ox.ac.uk)
- HLA-C-restricted CTL in chronic HIV infection are memory cells of an intermediate phenotype, characterized by high CD27 and low CD28 expression and lack of perforin production. (ox.ac.uk)
- For instance, current studies suggest that tumor-infiltrating B lymphocytes (TIL-B) can promote anti-tumor immunity through their unique antigen-presenting mode, leading to the persistence of an immune "hot" TME involving T cells, bone marrow cells, and natural killer cells [ 11 ]. (biomedcentral.com)
- Invariant natural killer T (iNKT) cells are CD1d-restricted T cells, which respond rapidly to antigen recognition and promote development of anti-tumor immunity in many tumor models. (biomedcentral.com)
- CD27 expression discriminates between regulatory and non-regulatory cells after expansion of human peripheral blood CD4+ CD25+ cells. (ox.ac.uk)
- We show that, of the markers associated with freshly isolated Treg, only CD27 expression correlated with regulatory activity and could be used to isolate cells with regulatory activity from lines expanded from CD4+ CD25+ cells. (ox.ac.uk)
- Expression of CD27 by cells in lines expanded from CD4+ CD25- cells varied depending on the stimulus used for expansion, but these lines did not have significant regulatory activity even when the CD27+ cells were tested. (ox.ac.uk)
- Analysis of synovial CD4+ CD25+ cells from reactive arthritis patients revealed that they were predominantly CD27 positive. (ox.ac.uk)
- We conclude that, whilst CD27 is useful for identifying Treg in the cell lines obtained after expansion of CD4+ CD25+ cells, its expression may not reliably identify the Treg cell population in other T-cell populations such as those found in joints. (ox.ac.uk)
- T cells displaying CD4 molecules (and not CD8 ) on their surface, therefore, are specific for antigens presented by MHC II and not by MHC class I (they are MHC class II-restricted ). (wikidoc.org)
- CD4 is a co-receptor of the T cell receptor (TCR) and assists the latter in communicating with antigen-presenting cells . (wikidoc.org)
Soluble CD271
- Soluble CD27 is an important prognostic marker of acute and chronic B cell malignancies. (thermofisher.com)
Ligand superfamily1
- Tumor necrosis factor ligand superfamily: involvement in the pathology of malignant lymphomas. (bdbiosciences.com)
Differentiation Antigens2
- 1989. Leucocyte Typing IV: White Cell Differentiation Antigens. (biolegend.com)
- 1995. Leucocyte Typing V: White Cell Differentiation Antigens. (biolegend.com)
Glycoprotein1
- Carcinoembryonic antigen (CEA) is a glycoprotein involved in cell adhesion . (wikidoc.org)
CD191
- Leukemia phenotype studies have demonstrated that the earliest and broadest B cell restricted antigen is the CD19 antigen. (thermofisher.com)
Lymphocytes4
- Human peripheral blood lymphocytes were stained with CD27 (clone M-T271) PE/Dazzle™ 594 (filled histogram) or mouse IgG1, κ PE/Dazzle™ 594 isotype control (open histogram). (biolegend.com)
- The CD27- subset of peripheral blood memory CD4+ lymphocytes contains functionally differentiated T lymphocytes that develop by persistent antigenic stimulation in vivo. (bdbiosciences.com)
- A group of heterogeneous lymphoid tumors generally expressing one or more B-cell antigens or representing malignant transformations of B-lymphocytes. (lookformedical.com)
- Lymphocytes proliferate and differentiate in response to various concentrations of different antigens. (thermofisher.com)
Proliferation3
- The TNF superfamily members are known for the regulation of cell proliferation and death. (thermofisher.com)
- CD27 interacts with TRAF family members, resulting in enhanced cellular proliferation and the induction of anti-apoptotic proteins. (stemcell.com)
- Cell Proliferation Induced by CD27 Ligand/TNFSF7 and Neutralization by Mouse CD27 Ligand/TNFSF7 Antibody. (bio-techne.com)
Flow cytometry1
- Flow cytometry analysis of C57BL/6 mouse splenocytes labeled with Anti-Mouse CD27 Antibody, Clone LG.3A10, followed by a goat anti-hamster (Armenian) IgG antibody, Alexa Fluor® 488 (filled histogram), or an Armenian hamster IgG isotype control antibody, followed by a goat anti-hamster (Armenian) IgG antibody, Alexa Fluor® 488 (solid line histogram). (stemcell.com)
Recombinant2
- Under these conditions, prolif-eration elicited by Recombinant Mouse CD27 Ligand/TNFSF7 (10 µg/mL) is neutralized (green line) by increasing concen-trations of Rat Anti-Mouse CD27 Ligand/ TNFSF7 Monoclonal Antibody (Catalog # MAB783). (bio-techne.com)
- The Neutralization Dose (ND 50 ) is typically 0.5-2 µg/mL in the presence of 10 µg/mL Recombinant Mouse CD27 Ligand/TNFSF7 and sub-optimal amounts of Mouse CD3 epsilon Monoclonal Antibody. (bio-techne.com)
ICOS1
- Another two stimulatory checkpoint molecules belong to the B7-CD28 superfamily-CD28 itself and ICOS. (wikipedia.org)
Humans2
- First, humans are generally tolerant to their own antigens. (coek.info)
- In humans, the carcinoembryonic antigen family consists of 29 genes, 18 of which are normally expressed. (wikidoc.org)
Disulfide-linked homodimer1
- The CD27 antibody recognizes a 110-120-kilodalton (kDa) disulfide-linked homodimer comprised of two 55-kDa polypeptide chains. (bdbiosciences.com)
Antibodies2
- This single-chain TNFSF (tumor necrosis factor superfamily) technology is superior to other biologics targeting TNFSF pathways, such as agonistic antibodies. (apogenix.com)
- Antibodies of the IgG sub-class are bi-functional molecules, possessing a F(ab) domain, variable in sequence and responsible for the binding of antigen, and an Fc domain, constant in sequence and responsible for mediating a range of antibody effector functions [ 1 ]. (bmj.com)
Subsets1
- In the current study it is shown that this NKp46 high phenotype was correlated with an increased expression of CD16 and CD27 compared to the CD8α + NKp46 - and NKp46 + NK-cell subsets in spleen and blood. (biomedcentral.com)
Activation2
- The cytoplasmic domains of CD27 have also been shown to interact with TRAF2 and TRAF5 to elicit NF-κB and SAPK/JNK activation. (biolegend.com)
- Camerini D, Walz G, Loenen WA, Borst J, Seed B. The T cell activation antigen CD27 is a member of the nerve growth factor/tumor necrosis factor receptor gene family. (bdbiosciences.com)
Monoclonal2
- The following product was used in this experiment: CD27 Monoclonal Antibody (OTI6D6), TrueMAB™ from Thermo Fisher Scientific, catalog # TA812372. (thermofisher.com)
- The American biotech company Celldex Therapeutics is working on CDX-1127, an agonistic anti-CD27 monoclonal antibody which in animal models has been shown to be effective in the context of T cell receptor stimulation. (wikipedia.org)
Expression1
- Hammarstrom S. The carcinoembryonic antigen (CEA) family: structures, suggested functions and expression in normal and malignant tissues. (wikidoc.org)
Costimulation1
- CD27 costimulation is known to suppress Th17 effector cell function. (wikipedia.org)
Viral1
- Instead of developing your own assays, explore our kits and services to quickly quantify viral antigens! (acrobiosystems.com)
Detects1
- Detects mouse CD27 Ligand/TNFSF7 in direct ELISAs. (bio-techne.com)