NMR and UV spectroscopic assays revealed that it encodes a novel polysaccharide lyase (PL), hereafter referred to as BtCDH, reflecting its source (B. thetaiotaomicron (Bt)) and its ability to degrade the GAGs CS, dermatan sulfate (DS), and hyaluronic acid (HA). (hal.science)
The structure of human dermatan sulfate epimerase 1 emphasizes the importance of C5-epimerization of glucuronic acid in higher organisms. (scilifelab.se)
Dermatan sulfate epimerase 1 (DS-epi1, EC 5.1.3.19) catalyzes the conversion of d-glucuronic acid to l-iduronic acid on the polymer level, a key step in the biosynthesis of the glycosaminoglycan dermatan sulfate. (scilifelab.se)
Heparan sulfate2
A method for the isolation and purification of peptidoglycan heparan sulfate (HS) from bovine liver is described. (rpi.edu)
Heparan sulfate (HS) proteoglycans (PGs) interact with a number of extracellular signaling proteins, thereby playing an essential role in the regulation of many physiological processes. (rpi.edu)
Proteoglycan3
The chondroitin lyases from Flavobacterium heparinum (Cytophaga heparinia) have been widely used in depolymerization of glycosaminoglycan and proteoglycan chondroitin sulphates. (rpi.edu)
Introduction: Aggrecan, a chondroitin sulfate proteoglycan encoded by the ACAN gene, is an important structural component of the cartilage matrix. (eurospe.org)
Previous: "A portion of connective tissue dominated by extracellular matrix containing collagen type II and large amounts of proteoglycan, particularly chondroitin sulfate[GO]. (virtualflybrain.org)
Glycosaminoglycan1
Chondroitin lyases (EC 4.2.2.4 and EC 4.2.2.5) are glycosaminoglycan-degrading enzymes that act as eliminases. (rcsb.org)
Keratan5
Keratan sulphate was isolated from adult intervertebral disc in 90% yield by sequential digestion of the whole tissue with papain, Pronase and Proteus vulgaris chondroitin sulphate lyase. (sahmri.org.au)
Treatment of this preparation with alkali cleaved a glycosidic bond between N acetylgalactosamine and threonine and produced, by an alkali catalyzed 'peeling' reaction, an unsaturated derivative of N acetylgalactosamine which reacted as a chromogen in the Morgan Elson reaction, but remained covalently bonded to the keratan sulphate chain. (sahmri.org.au)
The substituent at position 3 of N acetylgalactosamine in the keratan sulphate/protein linkage was identified as a disaccharide, N acetylneuraminylgalactose, which was isolated from the reaction mixture after alkali treatment. (sahmri.org.au)
Robinson, H. C. / The alkali labile linkage between keratan sulphate and protein . (sahmri.org.au)
Keratan sulfate (KS) was isolated from chicken egg white in amounts corresponding to ∼0.06 wt% (dry weight). (rpi.edu)
Heparin3
A novel type of heparinase (heparin lyase, no EC number) has been purified from Bacteroides stercoris HJ-15, isolated from human intestine, which produces three kinds of heparinases. (rpi.edu)
P. Datta, L. Yan, A. Awofiranye, J.S. Dordick, and R.J. Linhardt (2020), "Heparosan Chain Characterization: Sequential Depolymerization of E. Coli K5 Heparosan by a Bacterial Eliminase Heparin Lyase III and a Bacterial Hydrolase Heparanase Bp to Prepare Defined Oligomers", Biotechnol. (rpi.edu)
Exolyase1
Chondroitin sulfate ABC lyase may refer to: Chondroitin ABC lyase, an enzyme Chondroitin-sulfate-ABC exolyase, an enzyme Chondroitin-sulfate-ABC endolyase, an enzyme This disambiguation page lists articles associated with the title Chondroitin sulfate ABC lyase. (wikipedia.org)
AAV2 containing cDNA encoding enhanced green fluorescent protein (GFP), under the control of a chicken β-actin promoter, was delivered by intravitreal injection to adult mice in conjunction with enzymes including collagenase, hyaluronan lyase, heparinase III, or chondroitin ABC lyase. (molvis.org)
Polysaccharide2
BtCDH homologs were also detected in several other HGM species, and we therefore propose that it represents the founding member of a new polysaccharide lyase family (PL29). (hal.science)
Additionally, the structure of DS-epi1 reveals a high structural similarity to proteins from several families of bacterial polysaccharide lyases. (scilifelab.se)
Hyaluronan lyase had a limited effect and collagenase was ineffective. (molvis.org)
Enzymatic1
The significance of this work provides a better understand of sulfate groups on CNC and their potential effects on enzymatic hydrolysis of cellulose and surface modification of CNC. (auburn.edu)
Gene2
Here, we investigated a gene of unknown function (BT_3328) from the chondroitin sulfate (CS) utilization locus of B. thetaiotaomicron. (hal.science)
17 alpha hydroxylase, 17 20 lyase deficiency is an autosomal recessive inherited congenital adrenal hyperplasia type which is due to mutation in CYP17A1 gene and characterized with adrenal and gonadal sex steroid deficiency, delayed puberty in girls, XY sex development disorder in boys and hypergonadotrophic hypogonadism in both gender.Case: 15 year old girl referred to our clinic with vomiting, fatigue and muscle spasms. (eurospe.org)