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AgarImmunodiffusionGelsElectrophoresis, Agar GelElectrophoresisRats, HairlessElectrophoresis, Polyacrylamide GelElectrophoresis, CapillaryElectrophoresis, Gel, Two-DimensionalElectrophoresis, Gel, Pulsed-FieldPneumonia, Progressive Interstitial, of SheepImmunoelectrophoresisEnzootic Bovine LeukosisLeukemia Virus, BovinePrecipitinsGoatsComplement Fixation TestsGoat DiseasesEquine Infectious AnemiaMolecular WeightImmune SeraInfectious Anemia Virus, EquineHemoglobin CElectrophoresis, MicrochipLentivirus InfectionsArthritis-Encephalitis Virus, CaprineCattle DiseasesVisna-maedi virusAntibodies, ViralRhamnoseHorsesElectrophoresis, DiscReoviridaeElectrophoresis, Starch GelCattleEnzyme-Linked Immunosorbent AssayMolecular Sequence DataBlood Protein ElectrophoresisSheepRabbitsPrecipitin TestsMethodsCulture MediaEvaluation Studies as TopicElectrophoresis, Cellulose AcetateIsoelectric FocusingDenaturing Gradient Gel ElectrophoresisHemagglutination TestsCounterimmunoelectrophoresisSkin AbsorptionDNA, BacterialAntigens, BacterialSerologic TestsSensitivity and SpecificityCross ReactionsKineticsBase SequenceAmino Acid SequenceAntibodiesPolysaccharides, BacterialAntigens, ViralChromatography, GelBacterial Typing TechniquesBacterial ProteinsAntigensAmino AcidsHydrogen-Ion ConcentrationEscherichia coliPolymerase Chain ReactionMicrobial Sensitivity TestsIsoelectric PointDNA FingerprintingChromatography, AffinityChromatography, Ion ExchangeSodium Dodecyl SulfateSpecies SpecificityElectrophoresis, PaperProteomicsAnti-Bacterial AgentsSubstrate SpecificityAntibodies, BacterialProteomeTemperatureDNAChromatography, DEAE-CelluloseBacteriaSequence Analysis, DNACentrifugation, Density GradientSerotypingMacromolecular SubstancesTrypsinProteinsCell LineTwo-Dimensional Difference Gel ElectrophoresisRNA, Ribosomal, 16SChromatographyMutationChromatography, High Pressure LiquidCloning, MolecularPlasmids

Agarose gelsIsolated and purifiedPFGEFragmentsAdsorptionUltraviolet lightPercentage of agaroseNucleicBacteriophageElectrophoreticBufferParticlesFormed of helical agarose moleculesMelting temperatureAnionic groupsParticularly suitableConcentrationMolecular biologyExtraction KitSeparationElectroendosmosisForms a gelPurificationProteinMethodIsolatesGenePlateLaboratoryProductsApplicationsPlasmidMainMatrixMediumSequenceAnalysisStrengthHighFreePinkFactorsFastLiquidEasyCleanPointEnvironmentCellWaterGrowthEarly

Agarose gels3

• Most agarose gels used are between 0.7-2% dissolved in a suitable electrophoresis buffer. (wikipedia.org)
• pHast Pack™ ready-to-pour agarose gels are available in 1% agarose in TAE buffer or 1% agarose in TBE buffer . (sigmaaldrich.com)
• Simply mix with water, gently heat to dissolve the powder blend to quickly prepare reliable agarose gels for electrophoresis. (sigmaaldrich.com)

Isolated and purified1

• Agarose is a polysaccharide that is isolated and purified from agar or agar-bearing marine algae (sea kelp). (sigmaaldrich.com)

PFGE3

• The limit of resolution for standard agarose gel electrophoresis is around 750 kb, but resolution of over 6 Mb is possible with pulsed field gel electrophoresis (PFGE). (wikipedia.org)
• This relationship however breaks down with very large DNA fragments, and separation of very large DNA fragments requires the use of pulsed field gel electrophoresis (PFGE), which applies alternating current from different directions and the large DNA fragments are separated as they reorient themselves with the changing field. (wikipedia.org)
• Also various molecular typing techniques have been used, including pulsed-field gel electrophoresis (PFGE) and multilocus sequence typing (MLST), and these assays were considered as efficient techniques in MRSA typing but they are time consuming, expensive, and technically complex [ 8 ]. (hindawi.com)

Fragments5

• The proteins may be separated by charge and/or size (isoelectric focusing agarose electrophoresis is essentially size independent), and the DNA and RNA fragments by length. (wikipedia.org)
• The separated DNA may be viewed with stain, most commonly under UV light, and the DNA fragments can be extracted from the gel with relative ease. (wikipedia.org)
• Agarose gel has lower resolving power than polyacrylamide gel for DNA but has a greater range of separation, and is therefore used for DNA fragments of usually 50-20,000 bp in size. (wikipedia.org)
• A mixture of different sized DNA fragments was separated on an agarose gel (from 0.7 to 2% agarose in 1x TAE buffer and 0.1 µg/ml ethidium bromide) at a constant voltage of 100 V. (igem.org)
• The separation is not as good as PAGE electrophoresis, which can distinguish DNA fragments that differ by 1bp. (yeasenbiotech.com)

Adsorption3

• The removal of agaropectin in agarose substantially reduces the EEO, as well as reducing the non-specific adsorption of biomolecules to the gel matrix. (wikipedia.org)
• Biopsy tissue samples were extracted with phenol/chloroform (do not use BOOM because tissue samples often have a lot of DNA, so the target DNA cannot compete for adsorption on silica gel and also does not use BOOM for RNA extraction). (com.vn)
• Agarose gel electrophoresis has very little adsorption to the sample, so the electrophoresis pattern is clear, with high resolution and good repeatability. (yeasenbiotech.com)

Ultraviolet light2

• Read the results on the electrophoresis agar in the reading chamber under ultraviolet light. (com.vn)
• Agarose gel is transparent, without ultraviolet absorption, and the electrophoresis process and results can be directly detected and quantitatively determined by ultraviolet light. (yeasenbiotech.com)

Percentage of agarose1

• The percentage of agarose in the gel affects the size of the pores, which in turn affects the size and velocity of the molecules that can pass through. (yeasenbiotech.com)

Nucleic3

• Low EEO agarose is therefore generally preferred for use in agarose gel electrophoresis of nucleic acids, but high EEO agarose may be used for other purposes. (wikipedia.org)
• A number of factors can affect the migration of nucleic acids: the dimension of the gel pores (gel concentration), size of DNA being electrophoresed, the voltage used, the ionic strength of the buffer, and the concentration of intercalating dye such as ethidium bromide if used during electrophoresis. (wikipedia.org)
• Agarose is non-toxic and has several properties and specifications that make it useful as a gelling agent in many applications, such as nucleic acid electrophoresis, immunodiffusion techniques, gel plates or overlays for cells in tissue culture, cell culture media, gel chromatography, affinity chromatography, and ion exchange chromatography. (sigmaaldrich.com)

Bacteriophage2

• A 0.9% agarose gel has pores large enough for the entry of bacteriophage T4. (wikipedia.org)
• [ 2 ] Individual isolates can then be distinguished with serogrouping, pulsed-field gel electrophoresis, and bacteriophage serotyping techniques. (medscape.com)

Electrophoretic2

• Of these three electrophoretic techniques, agar gel is the easiest to interpret. (bmj.com)
• So in gel electrophoresis, the separation of charged particles depends not only on the nature and quantity of the net charge, but also on the size of the molecule, which greatly improves the electrophoretic resolution. (yeasenbiotech.com)

Buffer1

• 31. Tris-KCl buffer is a suitable solvent for the reaction, when the reaction tube is placed in the cycle chamber of the thermal cycler. (com.vn)

Particles1

• It can also be used to separate large proteins, and it is the preferred matrix for the gel electrophoresis of particles with effective radii larger than 5-10 nm. (wikipedia.org)

Formed of helical agarose molecules1

• Agarose gel is a three-dimensional matrix formed of helical agarose molecules in supercoiled bundles that are aggregated into three-dimensional structures with channels and pores through which biomolecules can pass. (wikipedia.org)

Melting temperature2

• The melting temperature is different from the gelling temperature, depending on the sources, agarose gel has a gelling temperature of 35-42 °C and a melting temperature of 85-95 °C. Low-melting and low-gelling agaroses made through chemical modifications are also available. (wikipedia.org)
• In other words, the gel point is not the same as the melting temperature. (sigmaaldrich.com)

Anionic groups2

• The anionic groups of the agarose gel are attached to the matrix and cannot migrate, while the dissociable countercations migrate to the cathode within the matrix to generate EEO. (sigmaaldrich.com)
• The anionic groups in the agarose gel adsorb on the matrix without migration, but the dissociated cations migrate toward the negative electrode, resulting in electroosmosis. (yeasenbiotech.com)

Particularly suitable3

• Agarose gel is easy to cast, has relatively fewer charged groups, and is particularly suitable for separating DNA of size range most often encountered in laboratories, which accounts for the popularity of its use. (wikipedia.org)
• Since the complex bldA phenotype is likely to involve elements of both transcriptional and translational control, it is particularly suitable for an integrated functional genomics approach. (biomedcentral.com)
• In addition, the extensively folded nature of MS2 RNA ( 22 ) may make it particularly suitable for uptake into the confines of a small capsid. (politicalespionage.com)

Concentration3

• Low-concentration gels (0.1-0.2%) however are fragile and therefore hard to handle. (wikipedia.org)
• Higher concentration gels would have higher electroendosmotic flow. (wikipedia.org)
• Electrophoresis agar, concentration from 1.5% -2% depending on the size of the cloned gene segment. (com.vn)

Molecular biology2

• Agarose gel electrophoresis is a method of gel electrophoresis used in biochemistry, molecular biology, genetics, and clinical chemistry to separate a mixed population of macromolecules such as DNA or proteins in a matrix of agarose, one of the two main components of agar. (wikipedia.org)
• DNA and RNA Manipulation: Glass Petri dishes can be used in various molecular biology techniques, such as polymerase chain reaction (PCR), gel electrophoresis, and DNA/RNA extraction and purification. (amarischemicalsolutions.com)

Extraction Kit2

• DNA was isolated from the gel slice with GeneJet Gel Extraction Kit according to the manufacturer's protocol. (igem.org)
• Desired PCR products were purified by GeneJet Gel Extraction Kit according to the manufacturer's protocol. (igem.org)

Separation6

• Since this method allows low-cost separation by automation of the separation process and by permitting reuse of the gel, industrial applications of separated metallic and semiconducting SWCNTs utilizing their characteristics are expected. (go.jp)
• Takeshi Tanaka (Research Scientist) and Hiromichi Kataura (Leader), the Self-Assembled Nano-Electronics Group, the Nanotechnology Research Institute (Director: Nobutsugu Minami) of AIST (President: Tamotsu Nomakuchi), developed a method for high-purity separation of SWCNTs into metallic SWCNTs and semiconducting SWCNTs by using a column packed with agarose gel beads, as a part of Industrial Technology Research Grant Program of NEDO. (go.jp)
• The gel-packed column can be used repeatedly, and the separation process can be easily automated. (go.jp)
• Gel electrophoresis is a common life science laboratory technique used to extract biological molecules based on their size, such as in DNA separation and DNA detection. (sigmaaldrich.com)
• Since electrophoresis of biopolymers is usually toward the anode, EEO can interfere with separation by internal convection. (sigmaaldrich.com)
• It is mainly used for DNA cutting gel recovery, DNA separation, and proving whether the DNA is recombined and whether the plasmid is cut successfully. (yeasenbiotech.com)

Electroendosmosis4

• Electroendosmosis is a reason agarose is used in preference to agar as the agaropectin component in agar contains a significant amount of negatively charged sulphate and carboxyl groups. (wikipedia.org)
• We provide a wide array of agarose products with varying gel strengths, melting temperatures, gelling temperatures, and electroendosmosis (EEO) levels to accommodate all of your research applications. (sigmaaldrich.com)
• Electroendosmosis (EEO) - Refers to the transfer of liquid through a gel. (sigmaaldrich.com)
• Electroendosmosis (EEO) - an electrokinetic movement of a liquid through a gel. (yeasenbiotech.com)

Forms a gel1

• Gel point - The temperature at which an aqueous solution of agarose forms a gel upon cooling. (sigmaaldrich.com)

Purification1

• Desired DNA fragment was excised and purified using suitable DNA purification kit. (igem.org)

Protein2

• Agarose gel has large pore size and good gel strength, making it suitable as an anticonvection medium for the electrophoresis of DNA and large protein molecules. (wikipedia.org)
• Learn your en- phase into a protein gel. (democo.de)

Method4

• This method involves the use of a column packed with agarose gel beads, in which the semiconducting SWCNTs are selectively adsorbed and then eluted (Fig. 1). (go.jp)
• All isolates were identified as D. citri by analyzing the sequences at the internal transcribed spacer (ITS) rDNA region using primers of ITS1/ITS4 or at β-tubulin using primer Btdcitri-F/R. Therefore, based on the present study, where the results of morphological identification of conidial type were consistent with DNA sequence analysis of certain gene, choosing a suitable method for a fast diagnosis of citrus melanose was suggested. (online-rpd.org)
• Agarose gel electrophoresis is an electrophoresis method that uses agarose as a support medium. (yeasenbiotech.com)
• The method is extremely suitable for quickly molecular etiologic screening and early diagnosis and aggressive prevention therapy of LVAS. (springeropen.com)

Isolates2

• Fewer than 1% of nontyphoidal Salmonella (NTS) isolates are lactose-positive (pink on MacConkey agar), but most produce hydrogen sulfide, which is detectable on HE or SS agar. (medscape.com)
• Among these fungal isolates, 40 representatives of D. citri isolates which were isolated from 22 twigs and 18 leaves on 23 farms were found based on cultural characteristics on potato dextrose agar and conidial morphology. (online-rpd.org)

Gene1

• Upon detection of environmental alterations, bacteria adjust their gene expression in order to obtain a phenotype that is suitable for the environment to which they are exposed ( Federle, 2012 ). (frontiersin.org)

Plate1

• A single colony was picked from a LB-agar plate or glycerol stock and inoculated in 10 mL of LB-medium with the appropriate antibiotic for selection (100 mg/L ampicillin, 50 mg/L kanamycin, 35 mg/L chloramphenicol). (igem.org)

Laboratory1

• Salmonellae can be isolated in the microbiology laboratory using numerous low-selective media (MacConkey agar, deoxycholate agar), intermediate-selective media ( Salmonella-Shigella [SS] agar, Hektoen [HE] agar), and highly selective media (selenite agar with brilliant green). (medscape.com)

Products2

• The products of PCR were separated by electrophoresis on 1.5% agarose gel. (aquaculturemag.com)
• For gel analysis, 20 μl of the PCR products were loaded in each gel slot. (aquaculturemag.com)

Applications1

• However, for some applications such as the electrophoresis of serum proteins, a high EEO may be desirable, and agaropectin may be added in the gel used. (wikipedia.org)

Plasmid1

• 10 g/L tryptone, 5 g/L yeast extract, 10 g/L NaCl, media is supplemented with suitable antibiotics depending on the selection marker on the transfected plasmid: ampicilin 100 mg/L or kanamycin 50 mg/L. (igem.org)

Main1

• What is main function of gel electrophoresis? (mcqexams.com)

Matrix3

• Biomolecules are separated by applying an electric field to move the charged molecules through an agarose matrix, and the biomolecules are separated by size in the agarose gel matrix. (wikipedia.org)
• In agarose gel electrophoresis, agarose is used as a matrix. (mcqexams.com)
• Agarose is the part of agar that forms a three-dimensional gel matrix composed of helical agarose molecules. (yeasenbiotech.com)

Medium2

• Electrophoresis means that the charged test sample swims in the direction of the corresponding electrode under the action line of the electric field in the inert support medium at different speeds, and the components will be separated into narrow zones. (yeasenbiotech.com)
• Seed Germination: Glass Petri dishes can be employed to germinate and observe the early growth stages of seeds before transferring them to a suitable planting medium. (amarischemicalsolutions.com)

Sequence1

• First, a suitable shRNA sequence must be identi﫿ed. (berterolab.com)

Analysis1

• Analysis of fragmented DNA was done by gel electrophoreses. (igem.org)

Strength2

• The pore size of a 1% gel has been estimated from 100 nm to 200-500 nm, and its gel strength allows gels as dilute as 0.15% to form a slab for gel electrophoresis. (wikipedia.org)
• Gel strength - The force that must be applied to the gel to fracture it. (sigmaaldrich.com)

High1

• Diaphorobacter nitroreducens and Achromobacter mucicolens are suitable for carbamazepine removal while both, Pseudomonas veronii and Pseudomonas lini show high siderophore production and phosphate solubilization. (frontiersin.org)

Free1

• Electrophoresis mainly includes free electrophoresis and zone electrophoresis. (yeasenbiotech.com)

Pink1

• When SWCNTs dispersed in a solution were applied to the column, reddish metallic SWCNTs passed through the gel (pink bar), while green semiconducting SWCNTs (green bar) were adsorbed onto the gel. (go.jp)

Factors1

• 3. What are the factors affecting agarose gel electrophoresis? (yeasenbiotech.com)

Fast1

• Agarose gel electrophoresis is simple and fast, and samples can be electrophoresed without prior treatment. (yeasenbiotech.com)

Liquid1

• Agarose solutions exhibit hysteresis when transitioning from liquid to gel. (sigmaaldrich.com)

Easy1

• After electrophoresis, the zone is easy to stain, the sample is easily eluted, and it is convenient for quantitative determination. (yeasenbiotech.com)

Clean1

• The band with the desired DNA fragment was excised from the gel, using a clean scalpel. (igem.org)

Point1

• The lower sulphate content of low EEO agarose, particularly low-melting point (LMP) agarose, is also beneficial in cases where the DNA extracted from gel is to be used for further manipulation as the presence of contaminating sulphates may affect some subsequent procedures, such as ligation and PCR. (wikipedia.org)

Environment1

• Nutrient agar or other growth media can be added to the dish, providing a suitable environment for the microorganisms to proliferate. (amarischemicalsolutions.com)

Cell1

• Agar gel, cellulose acetate, and starch gel electrophoresis are all capable of diagnosing sickle-cell anaemia, sickle-cell haemoglobin C disease, and haemoglobin C disease in cord blood samples. (bmj.com)

Water2

• Agarose gel is prepared by dissolving agarose in hot water and cooling the solution until it solidifies. (go.jp)
• The yucca has been suggested suitable for water quality management in aquaculture systems. (aquaculturemag.com)

Growth1

• Using a self-stimulated neural conductive channel to create a suitable scaffold for the growth and differentiation of neural-like cells can be desirable20. (yjxinhua.com)

Early1

• In addition, surgical resection and liver transplantation are restricted, and are suitable only for patients diagnosed with early stage disease ( 6 ). (spandidos-publications.com)