• The double transgenic mice (dTg) were obtained by mating: (i) transgenic mice expressing the hemagglutinin of influenza virus under the insulin promoter with (ii) transgenic mice expressing specific T lymphocytes with receptor for the immunodominant epitope of the same virus. (promisekit.org)
  • Tg2576 mice overexpress a mutant form of human amyloid precursor protein with the Swedish mutation (APP Sw ), resulting in high β-amyloid (Aβ) levels in the brain. (jneurosci.org)
  • Despite this, amyloid plaques do not develop until 12 months of age, and there is no neuronal loss in mice as old as 16 months. (jneurosci.org)
  • The expression of transthyretin, a protein shown to sequester Aβ and prevent amyloid fibril formation in vitro , and several genes in the insulin-signaling pathway, e.g., insulin-like growth factor-2, were increased selectively in the hippocampus of APP Sw mice. (jneurosci.org)
  • ANAVEX®3-71 demonstrated disease-modifying activity against the major hallmarks of Alzheimer's disease in transgenic (3xTg-AD) mice, including cognitive deficits, amyloid and tau pathologies, as well as beneficial effects on mitochondrial dysfunction and neuroinflammation. (anavex.com)
  • vascular amyloid deposition) in HHcy APP/PS1 mice. (biomedcentral.com)
  • Interestingly, after 6 months of HHcy, the APP/PS1 mice were cognitively worse than wild-type HHcy mice or APP/PS1 mice, indicative of an additive effect of the cerebrovascular pathology and amyloid deposition. (biomedcentral.com)
  • Minocycline is a semi-synthetic second-generation tetracycline known to improve cognition in amyloid precursor protein transgenic mice. (proteasomesignaling.com)
  • When these encapsulated cells were implanted, before the onset of amyloid plaque deposition in TauPS2APP mice, their antibodies dramatically reduced beta-amyloid levels in the brain, decreased amyloid plaque burden, and prevented phospho-tau pathology in the hippocampus. (medscape.com)
  • Gene expression profiles in the hippocampus and cerebellum of 6-month-old APP Sw mice were compared with age-matched controls. (jneurosci.org)
  • Therefore, we were interested in early gene expression changes that might mediate neuroprotection in these mice. (jneurosci.org)
  • Thus the present study was designed to determine gene expression profiles in the hippocampus and cerebellum of 6-month-old APP Sw mice compared with age-matched controls and to identify potential mechanisms responsible for protecting neurons from Aβ toxicity. (jneurosci.org)
  • Using HLA-A2.1 transgenic mice, we have demonstrated the feasibility of T-cell receptor (TCR) gene transfer into T cells to circumvent self-tolerance to the widely expressed human p53 and MDM2 TAAs. (unimedizin-mainz.de)
  • Here, we evaluated the safety issues raised by the risk of TAA-TCR gene transfer-associated on/off-target toxicities and the therapeutic potential in relevant transgenic mouse models of adoptive transfer. (unimedizin-mainz.de)
  • To address the question of specificity for the allele encoding the Na V 1.4-R669H variant as a cause of HypoPP and to produce a model system in which to characterize functional defects of the mutant channel and susceptibility to paralysis, we generated knockin mice carrying the ortholog of the gene encoding the Na V 1.4-R669H variant (referred to herein as R669H mice). (jci.org)
  • Transgenic mice that were created that had a mutation in the gene which codes for the transcription factor (NGFIB). (freeonlineresearchpapers.com)
  • It was hypothesized that a growth hormone gene would speed up and increase overall growth of the mice. (freeonlineresearchpapers.com)
  • The key difference between transgenic and gene targeted (knockout or knockin) animals is that in knockout and knockin models the genomic changes are targeted, meaning a desired DNA sequence is altered at a specific locus in the target genome via homologous recombination, with an engineered segment of DNA replacing a homologous endogenous segment. (cyagen.com)
  • To generate a transgenic mouse, microinjection of DNA or infection with a viral vector containing the gene construct can be used to insert the gene of interest into a zygote or into embryonic stem cells. (promisekit.org)
  • To do this they genetically-engineered mice by adding a fluorescent gene from fireflies to them. (promisekit.org)
  • Scientists at Seoul National University, led by stem cell researcher Byeong-Chun Lee, successfully created a set of transgenic puppies: beagles which carry a red fluorescent gene from glowing sea anemones. (ebaumsworld.com)
  • After four years of research and validation, GemPharmatech's independently developed fully antibody gene humanized mouse model, NeoMab™, is officially entering the market to serve the therapeutic antibody development needs of biotechnology companies and pharmaceutical enterprises. (gempharmatech.us)
  • It retains the native mouse constant region encoding genes while incorporating human variable gene repertoires at the endogenous loci. (gempharmatech.us)
  • NeoMab™ mice utilize human V(D)J genes to encode antibodies, exhibiting gene usage frequency and sequence diversity that closely resemble those of humans. (gempharmatech.us)
  • Our team has successfully developed an efficient inducible spCas9 gene editing system in hPSCs, achieving a knockout efficiency of over 90% for the target genes. (eemea.org)
  • Using this advanced editing system, we have successfully generated a Barth Syndrome hPSCs-CMs model by knocking out the Tafazzin gene. (eemea.org)
  • Lastly, we have conducted in vivo gene therapy research using the base editor on transgenic mice with hypertrophic cardiomyopathy and hereditary arrhythmias respectively. (eemea.org)
  • To study the association between dietary fat composition and liver neovasculogenesis, the researchers fed hepatitis C virus core gene transgenic (HCVcpTg) mice with a control diet or with TFA-/SFA-/cholesterol-rich diets containing the same number of calories. (toppersonalhealth.com)
  • Could another research team overcome these problems and successfully implant human genes into pigs or cattle or goats or chickens? (greens.org)
  • Edible vaccines are subunit vaccines where the selected genes are introduced into the plants and the transgenic plant is then induced to manufacture the encoded protein. (kindness2.com)
  • For this reason, genes have been cloned and expressed in animals such as sheep, goats, chickens, and mice. (opentextbc.ca)
  • How are genes inserted into mice? (promisekit.org)
  • These results suggest that the slow progression and lack of full-fledged Alzheimer's disease pathology in the hippocampal neurons of APP Sw mice result from the genetic reprogramming of neural cells to cope with increased levels of Aβ. (jneurosci.org)
  • Here, we constructed a whole-brain, three-dimensional (3D) map showing the tract pathways and targeting locations of these four motor cortical outputs in mice. (nature.com)
  • Biomarker analysis revealed that ANAVEX®3-71 prevents McGill-APP transgenic rats from increasing cortical (p ≤ 0.05) and hippocampal (p ≤ 0.01) extracellular Aβ deposition. (anavex.com)
  • To investigate whether sheep infected with scrapie prions could be another source of infection, we inoculated transgenic mice that overexpressed human prion protein with brain tissue from sheep with natural field cases of classical and atypical scrapie, sheep with experimental BSE, and cattle with BSE. (cdc.gov)
  • The researchers first inoculated the brains of hamsters and humanized transgenic mice with rodent or human prion samples, respectively. (case.edu)
  • Furthermore, we demonstrate that wholemount adult mouse brains processed with EZ Clear can be successfully immunolabeled for fluorescent imaging while still retaining signal from endogenous fluorescent reporters. (elifesciences.org)
  • By two-dimensional gel electrophoretic analysis of synaptosomal fractions from transgenic mouse brains we detected additional isoforms of septin 6, a downstream target of Cdc42 effector proteins. (proteasomesignaling.com)
  • We will test our models of host range (i.e. emergence potential) experimentally using reverse genetics, pseudovirus and receptor binding assays, and virus infection experiments in cell culture and humanized mice. (substack.com)
  • First transgenic experiments were carried out on mice. (freeonlineresearchpapers.com)
  • Using experiments involving transgenic mice, they have successfully shown that dietary saturated/trans fats, but not cholesterol, can trigger hepatic angiogenesis and lymphangiogenesis, leading to the promotion of hepatic tumors. (toppersonalhealth.com)
  • To study the consequences of sustained exposure to Ex-4, we generated metallothionein promoter-exendin-4 (MT-Exendin) mice that continuously express a proexendin-4 transgene in multiple murine tissues. (diabetesjournals.org)
  • This includes free strategy design prior to your purchase, construction of transgenic or targeting vectors (including BAC recombineering), creating and characterizing targeted ES cells, microinjection of mouse or rat embryos, and breeding and genotyping of the resulting animals. (cyagen.com)
  • MT-Exendin mice generate antibodies directed against Ex-4, exendin NH 2 -terminal peptide (ENTP), and proexendin-4 as well as antibodies that cross-react with native GLP-1. (diabetesjournals.org)
  • According to the scientist, it is also conceivable to generate T cell receptor transgenic cell therapies against patient-individual cancer antigens. (dkfz.de)
  • Simultaneously, we also anticipate that the successfully generated transgenic mice containing floxed allele of HAX-1 cross with other tissue-specific Cre mice and generate non-kidney tissue-specific-knock-out offspring, providing an excellent tool for studying signaling and functional effects in certain tissues or organs excluding the kidney. (tmu.edu.tw)
  • To test this possibility, we administered a diet to induce HHcy in APP/PS1 transgenic mice. (biomedcentral.com)
  • Since MR disruption from early development on may induce compensatory mechanisms, new transgenic mice should be developed allowing for an inducible and reversible deletion of the MR in the hematopoietic system. (uni-goettingen.de)
  • We successfully applied this strategy to the human TAAs p53, gp100 and MDM2-specific TCRs as promising antigens-driven immunotherapy for both melanoma and hematologic malignancies. (unimedizin-mainz.de)
  • After immunization with human recombinant protein antigens, NeoMab™ mice display antigen-specific serum titers similar to BALB/c mice, reaching approximately 10 5 -10 6 after three immunizations. (gempharmatech.us)
  • In 1997, Japanese researchers reported inserting a complete human chromosome into mice to produce human antibodies. (greens.org)
  • Antibodies obtained from NeoMab™ mice demonstrate an affinity of approximately 10 -10 to 10 -8 (as determined by SPR detection), which is comparable to or even higher than that of FDA-approved therapeutic antibody drugs. (gempharmatech.us)
  • The first method involves DNA delivery by retroviral infection of mouse embryos at different developing stages. (promisekit.org)
  • After TCR identification, we generated HLA-A*02:01/peptide restricted TCR transgenic T cells by retroviral transduction and tested T cell expansion rates as well as A*02:01/peptide recognition and ES killing in ELISpot and xCELLigence assays. (oncotarget.com)
  • In the new study, Zou and colleagues successfully detected prions in rodent skin samples as early as two weeks post-infection. (case.edu)
  • Our results demonstrated that the Y-5A15 remarkably enhanced the Aβ epitope immunogenicity and elicited high antibody titers against Aβ in AD mice. (mdpi.com)
  • They were produced at high titers, injected into fertilized mouse oocytes and transgenic offspring was identified that had integrated the new vector. (uni-goettingen.de)
  • The mice carry a temperature-sensitive variant of the SV40 large T antigen under control of the IFN-g-inducible H-2kb promoter. (cls.shop)
  • Furthermore, lymphocytes isolated from MT-Exendin mice proliferate in response to proexendin-4 but not after exposure to Ex-4 or ENTP. (diabetesjournals.org)
  • This grant proposed the conduct of pre-clinical work to support the use of stem cells to regenerate a cancer-fighting immune system in mice and humans, and bedside-to-bench work to analyze populations of cells with potential ability to function as long term repopulating T lymphocytes obtained from patients treated within a phase 1 clinical trial. (ca.gov)
  • For enhancer testing in mouse embryos using the LacZ reporter, we also offer LacZ staining of embryos. (cyagen.com)
  • How many embryos are injected for transgenic production, and what is the success rate? (cyagen.com)
  • Although several recombinant proteins used in medicine are successfully produced in bacteria, some proteins need a eukaryotic animal host for proper processing. (opentextbc.ca)
  • These mice, therefore, may not be a good model of the complete pathologic process of AD but rather may be an excellent model for understanding how the brain can adapt to and survive high levels of Aβ. (jneurosci.org)
  • have been successfully leveraged in various biological model systems. (elifesciences.org)
  • In this disease model, the mutant mice suffer significantly less from EAE than control mice. (uni-goettingen.de)
  • The advantage of such mice is that they provide a model which closely mimics the human body. (promisekit.org)
  • This strategy results in a powerful model that combines the strengths of the mouse immune system with human-specific variable regions, making it ideal for advancing antibody discovery and research endeavors. (gempharmatech.us)
  • At present, we have established extensive repositories of model resources, encompassing KOAP mice and tool mice for drug screening purposes. (gempharmatech.us)
  • By incorporating a human hepatitis-B transgene and inducing mutagenesis via a sleeping-beauty-transposon system, a sophisticated liver cancer model has been created to study human hepatocellular carcinoma in mice. (ismrm.org)
  • In conclusion, COS could significantly improve the ovarian and immune function of chemotherapy model mice, and improve the survival rate of OGSCs, which provided a preliminary blueprint for further exploring the mechanism of COS in protecting ovarian function. (biomedcentral.com)
  • Our studies demonstrate that prolactin can promote diverse carcinomas in mice, many of which resemble luminal breast cancers, providing a novel experimental model to examine the pathogenesis, progression and treatment responsiveness of this tumor subtype. (biomedcentral.com)
  • In paper №1, I detail the neurodegenerative and behavioural outcomes in a mouse lesion model of PD, which can be used as a platform for the development of novel therapeutic strategies. (lu.se)
  • In paper №2, I describe how mesDA neurons transplanted in the adult SN of a PD mouse model, extended axons across millimetres into the striatum, functionally reforming the nigrostriatal pathway. (lu.se)
  • ANAVEX®3-71 (10 μg/kg) or control (saline) was administered orally once daily in pre-plaque McGill-R-Thy1-APP transgenic (Tg) rats (n=22) and wild-type (wt) rats (n=22) divided into four groups, for seven months. (anavex.com)
  • By contrast, in transgenic animals an engineered segment of DNA is randomly integrated, such that the inserted DNA could end up almost anywhere in the host genome, and does not normally disrupt the homologous endogenous sequence. (cyagen.com)
  • 24h after TCR transduction, CD107a expression correlated with low expansion rates due to apoptosis of ADRB3 specific T cells in contrast to CHM1 specific transgenic T cells. (oncotarget.com)
  • Comparison of peptide motive binding affinities revealed extended fratricide among ADRB3 295 specific TCR transgenic T cells in contrast to CHM1 319 . (oncotarget.com)
  • One approach involves the experimental transmission of disease by inoculating homogenized brain tissue from affected animals into transgenic mice that are overexpressing 1 of the 2 common polymorphic forms of the human PrP (either methionine or valine at residue 129) on a mouse PrP null background ( 16 ). (cdc.gov)
  • Brain tumor-bearing mice treated with transgenic NLGN4X-specific human T cells had a greater than 40 percent response to treatment. (dkfz.de)
  • We will then use receptor-mutant pseudovirus binding assays, in vitro studies in bat, primate, human and other species' cell lines, and with humanized mice where particularly interesting viruses are identified phylogenetically, or isolated. (substack.com)
  • Gen Pharm bioengineered Herman, the first transgenic dairy bull, for producing milk with a human protein. (greens.org)
  • You might have seen the infamous Vacanti Mouse in the late 90's-early 2000s, a mouse which had a human ear grown on its back -- this is not that. (ebaumsworld.com)
  • We also detected elevated levels of vascular endothelial growth factor C and fibroblast growth factor receptors 2 and 3, as well as c-Jun N-terminal kinase and hypoxia-inducible factor 1α in the mice livers. (toppersonalhealth.com)
  • The NeoMab™ mouse is developed on the widely used BALB/c genetic background, making it highly suitable for antibody discovery in the industry. (gempharmatech.us)
  • The immune system of NeoMab™ mice remains intact, with proportions of various immune cell subsets similar to those of the BALB/c mice. (gempharmatech.us)
  • NeoMab™ mice exhibit normal antibody class switching, somatic hypermutation, and B-cell development, with comparable levels of immunoglobulin in their serum to BALB/c background mice. (gempharmatech.us)
  • With their new custom antibody from CRB they were able to successfully identify transcription targets of RORα. (crbdiscovery.com)
  • We now report that MT-Exendin mice develop extensive tissue lymphocytic infiltration with increased numbers of CD4 + and CD8a + cells in the liver and/or kidney and increased numbers of B220 + cells present in the pancreas and liver. (diabetesjournals.org)
  • Lukas Bunse, a scientist at the German Cancer Research Center (Deutsches Krebsforschungszentrum, DKFZ) and a physician at the University Medical Center Mannheim, relies on the comparatively new concept of "T cell receptor transgenic cells" in his current study: To this end, brain tumor patients were first inoculated with an antigenic fragment of the protein NLGN4X (Neuroligin4X). (dkfz.de)
  • In melanoma, the malignant black skin cancer, such T-cell receptor transgenic T cells have already been shown to prolong the lives of some patients. (dkfz.de)
  • Much more advanced in clinical development than T-cell receptor transgenic cells are cellular therapies using so-called CAR-T (chimeric antigen receptor) cells, which have already been approved for the treatment of various leukemias and lymphomas. (dkfz.de)
  • We have also advanced our studies to test the transplantation of hematopoietic stem cells (HSC) genetically engineered to express T cell receptors (TCR) and provide a continuous progeny of TCR transgenic mature T cells in humanized mouse models. (ca.gov)
  • Researchers at Sichuan University have developed a way to manufacture ears INSIDE mice by injecting them with a "bio-ink" made of hydrogel particles and cartilage cells and then shining ear-shaped light patters onto the injection site. (ebaumsworld.com)
  • At present, the cells have been cultured successfully for more than 40 passages without noting phenotypic changes. (cls.shop)
  • We hypothesized lysosome-associated membrane glycoprotein 1 (LAMP1, CD107a) to be a marker for fratricide in TCR transgenic CD8 + T cells. (oncotarget.com)
  • Results were compared to CHM1 319 -TCR transgenic T cells. (oncotarget.com)
  • TCR transgenic T cells demonstrated HLA-A*02:01/ADRB3 295 mediated ES recognition and killing in ELISpot and xCELLigence assays. (oncotarget.com)
  • Amino-acid exchange scans clearly indicated the cross-reactive potential of HLA-A*02:01/ADRB3 295 - and HLA-A*02:01/CHM1 319 -TCR transgenic T cells. (oncotarget.com)
  • The 2A sequence remains associated with RORα following expression in transgenic reporter mice (Rora Teal/Teal ). (crbdiscovery.com)
  • In addition, the increased expression of transthyretin and insulin-like growth factor-2 and the increased phosphorylation of Bad in hippocampal neurons were maintained in 12-month-old APP Sw mice when compared with age-matched controls. (jneurosci.org)
  • In wild-type mice, the activation of specific hindlimb muscles is increased when animals switch from walking to swimming. (elifesciences.org)
  • We also found that HHcy induced more microhemorrhages in the APP/PS1 mice than in the wild-type mice and that it switched the neuroinflammatory phenotype from an M2a biased state to an M1 biased state. (biomedcentral.com)
  • In a ground-breaking study published in Nature Communications , Zou and an international team of researchers successfully used two methods to detect prions in skin samples collected from inoculated rodents. (case.edu)
  • Several methods have been developed and successfully applied for a vast range of applications. (rutgers.edu)
  • we analyze some formal properties of such variants, compare them with other methods proposed in the literature and describe an effective randomized heuristic algorithm that has been developed and successfully applied for the solution of large scale feature selection problems. (rutgers.edu)
  • The formation of extracellular plaques is common to all lines of transgenic mice overexpressing high levels of mutant APP. (jneurosci.org)
  • In this study, transgenic and nontransgenic control mice were generated from C57B6/SJL N2 generation Tg2576 mice backcrossed to C57B6/SJL breeders. (jneurosci.org)
  • The study successfully reached primary and secondary safety endpoints. (anavex.com)
  • In the present study, we induced HHcy in APP/PS1 transgenic mice. (biomedcentral.com)
  • Here, we report a simple and rapid tissue clearing method, EZ Clear, that can clear whole adult mouse organs in 48 hr in just three simple steps. (elifesciences.org)
  • 6 ] successfully isolated OGSCs from the ovaries of pups and adult mice for the first time, transfected them with GFP virus in an appropriate environment in vitro, and then transplanted them into the ovaries of immunodeficient mice. (biomedcentral.com)
  • In both hamsters and mice, the researchers detected prions in skin before they could be detected in brain tissue. (case.edu)
  • Researchers then stitched these lab-grown into the eyes of mice, because research isn't fun when the mice can't cry. (ebaumsworld.com)
  • These findings demonstrate that expression of a proexendin-4 transgene may be associated with activation of humoral and cellular immune responses in mice. (diabetesjournals.org)
  • Scientists from the German Cancer Research Center (DKFZ) and the University Medical Center Mannheim (UMM) successfully tested a new form of cellular immunotherapy against brain tumors in mice for the first time. (dkfz.de)
  • All four women received the implant successfully and report full working order. (ebaumsworld.com)
  • We found that these mice were susceptible to BSE prions, but disease did not develop after prolonged postinoculation periods when mice were inoculated with classical or atypical scrapie prions. (cdc.gov)
  • Macrophages both from the spinal cord and the peritoneal cavity of diseased MRlysMcre mice show a polarization towards the AAM phenotype. (uni-goettingen.de)
  • When such nucleases (or their DNA or mRNA precursors) designed to target a specific site in the mouse or rat genome are microinjected into fertilized eggs, cleavage at the target site followed by imperfect repair can result in small deletions (and insertions, more rarely) of one or more base pairs. (cyagen.com)
  • Non-homologous recombination incorporates this DNA randomly into the mouse genome. (promisekit.org)
  • This is supported by in vivo results obtained by inducing experimental autoimmune encephalomyelitis (EAE) mimicking multiple sclerosis (MS) in MRlysMcre and MRflox/flox mice. (uni-goettingen.de)
  • However, the molecular mechanism physiology role of HAX-1 in cisplatin-induced nephrotoxicity is still obscured in vivo as a result of death of HAX-1 knock out mice as early as five weeks after breath. (tmu.edu.tw)
  • Cyagen's top priority is to provide high quality mouse and rat models. (cyagen.com)
  • We offer all the services necessary to create your desired mouse or rat models. (cyagen.com)
  • GemPharmatech exemplifies dedication in furnishing mouse models and support services for the advancement of disease mechanism research, pharmaceutical development, and translational studies. (gempharmatech.us)
  • Here, we designed a yeast-based vaccine Y-5A15 comprising five copies of Aβ1-15 displayed on the surface of yeast cell wall, and we subcutaneously immunized APP/PS1 mice three times. (mdpi.com)
  • The SVI cell line has been cloned from the outgrowth of glomeruli which were isolated from H-2kb-tsA58 transgenic mice. (cls.shop)
  • Says Prof. Tanaka, "Long-term feeding of SFA and TFA diets to HCVcpTg mice increased the expressions of vascular endothelial cell indicators like CD31 and TEK receptor tyrosine kinase, in addition to lymphatic vessel endothelial hyaluronan receptor 1. (toppersonalhealth.com)
  • I also describe the development of a novel behavioural task that is predictive of mesDA neuron cell loss in mice. (lu.se)
  • We pride ourselves on our ability to supply specific-pathogen free mice to animal facilities around the world. (cyagen.com)
  • Therefore, in this proposal, we aim to elucidate the following problems: (1) What is the physiology role of HAX-1 following cisplatin-induced kidney injury in kidney specific conditional HAX-1 knock out mice (HAX-1KSP-/-) or in WT mice? (tmu.edu.tw)
  • Histological analysis of MRlysMcre mice suffering from EAE confirmed that the pathophysiological features were less severe compared to control mice. (uni-goettingen.de)
  • Laparoscopic Roux-en-Y gastric bypass surgery (LRYGB) is a complex and high-risk procedure with technical challenges. (bvsalud.org)
  • We have successfully competed for a CIRM disease team award and have gone through a pre-IND meeting with the FDA to adequately plan for such a clinical trial to be started in approximately two years. (ca.gov)
  • In 2004, Johnson [ 4 ] and others found that the atresia rate of mouse follicles was significantly higher than the decrease of non-atretic follicles in follicle counting. (biomedcentral.com)
  • The most common fields in transgenic livestock that are being researched are: growth, pathogen resistance and quality of meat products. (freeonlineresearchpapers.com)
  • Conditional kidney HAX-1 knock out mice (HAX-1 KSP-/-) mice and restoration of HAX-1 in HAX-1 KSP-/- mice by lentivirus (gain of function) both will be generated and subjected to cisplatin injury in kidney. (tmu.edu.tw)
  • These mice were generated as a laboratory tool to better understand the onset and progression of cancer. (promisekit.org)