Successfully established tissue cultures. Medical search. Frequent questions

Adult stem cell-based organoid technology is a versatile tool for the generation and long-term maintenance of near-native 3D epithelial tissues in vitro. (nature.com)
Here, we provide an overview of the protocols used by different groups to establish organoids from various epithelial tissues and cancers, plus the different protocols subsequently used to test the in vitro therapy sensitivity of these patient-derived organoids. (nature.com)
Mouse endometrial epithelial cells (MEECs) were isolated from C57BL female mice, and long-term cultures of MEECs were established and maintained with conditional reprogramming (CR) method. (biomedcentral.com)
The researchers deployed two techniques to confirm that the organoid mimics the epithelial (outer) tissue of the bat's intestine-the part of the bat organ that first encounters virus particles and is thus of particular scientific interest. (techexplorist.com)
To optimize the ASC-supported culture, freshly isolated limbal epithelial cells in the form of single cells (SC-ASC) or cell clusters (CC-ASC) were cultured using three different methods: LSCs seeded directly on feeder cells, a 3-dimensional (3D) culture system and a 3D culture system with fibrin (fibrin 3D). (drcremers.com)
CC-ASC generated epithelial cells with undifferentiated morphology in all culture methods, among which CC-ASC in 3D culture supported the highest cell doubling (cells doubled 9.0 times compared to cells doubled 4.9 times in control) while maintained the percentage of putative limbal stem/progenitor cells compared to the control. (drcremers.com)

The drug discovery process requires that upwards of 10,000 molecules be screened for each new lead compound developed and relies upon an expensive and time consuming combination of in vitro cell culture-based and in vivo whole animal-based models to identify, validate, and ensure the safety of any resulting potential therapeutic agents. (inknowvation.com)
This process is frustratingly exacerbated by an unfortunate dichotomy whereby the inexpensive in vitro cell culture systems used for tier 1 screening contribute to failures due to their inabiliy to model the complexity and parallel systems interaction inherent in whole animal models (which are responsible for up to 92% of new compound failures at the clinical level) and because they are not capable of demonstrating species-specific effects. (inknowvation.com)
Employing in vitro cell culture of inherited mitochondrial disease and an in vivo animal experimental model of low-grade inflammation (high fat feeding), we show human-derived MSCs to alter mitochondrial function. (frontiersin.org)

To investigate matrix metalloproteinase-2 (MMP-2) and tissue inhibitor of metalloproteinase-2 (TIMP-2) expressions in the cultured rat mesangial cells (MsC) transfected with Smad 7 vector and to elucidate the mechanism of Smad 7 in blocking tissue fibrosis. (eurekamag.com)
It is possible that Smad 7 can alleviate the development of tissue fibrosis by upregulating the expression of MMP-2 and downregulating the expression of TIMP-2 in mesangial cells. (eurekamag.com)
Both 2D monolayer and 3D neurospheres were successfully cultured and characterized for proliferative potential, intrinsic plasticity, presence of cancer stem cells and the expression of stem cell markers. (frontiersin.org)
Cells cultured as 3D were embedded as tissue blocks. (frontiersin.org)
A spontaneous cell line-like cell culture with high percentage of stem cells has been established from a DIG tumor for the first time. (frontiersin.org)
The tumor is heterogeneous with meningeal tumor cells, mainly a mixture of fibroblast like cells, and neuroepithelial cells in a background of connective tissue. (frontiersin.org)
Identification of multipotent luminal progenitor cells in human prostate organoid cultures. (nature.com)
However, in the absence of morphological characterization of those cells in culture, the application of such models is delayed. (fiocruz.br)
Primary cultures were successfully established from nine Biomphalaria tissues and the respective cells in culture were ultra structurally described. (fiocruz.br)
Attention was particularly devoted to cells derived from mantle cavity and kidney tissues. (fiocruz.br)
Moreover, under optical microscopy, kidney cells in culture were very similar to those derived from amebocyte-producing organ (APO) cultures, which have been recently shown to be capable of eliminating S. mansoni sporocysts in vitro. (fiocruz.br)
Based on the close resemblance of those cultures and their anatomical proximity inside the mantle cavity, we suggest the effective participation of Biomphalaria kidney cells in hematopoiesis and in host response to S. mansoni infection. (fiocruz.br)
Finally, human umbilical vein endothelial cells (HUVECs) were initially cultured and maintained for at least three days as first proof-of-concept experiment. (utwente.nl)
Bone marrow and adipose tissue have emerged as excellent sources of progenitor cells. (ivis.org)
Stem cells are known to work via a variety of methods to heal tissue depending on where the stem cells come from and into which tissue it is being placed. (drcremers.com)
Here is a general list of all the Ways Stem Cells heal tissues or create new tissue. (drcremers.com)
1. Stem cells have long been known to have the ability to differentiate (ie become/transform) into "good/normal" tissue. (drcremers.com)
Embryonic stem cells have been fraught with difficulties in coaxing them into "good/normal" tissue and there are major ethical issues. (drcremers.com)
The most efficient method to expand limbal stem cells (LSCs) in vitro for clinical transplantation is to culture single LSCs directly on growth-arrested mouse fibroblast 3T3 cells. (drcremers.com)
The standard method to culture LSCs on 3T3 feeder cells that have been used in clinical study is cultivating single LSC directly on top of the growth-arrested 3T3 feeder cells [ 14 ]. (drcremers.com)
From human skin cells to three-dimensional cultures, induced pluripotent stem cell (iPSC)-derived organoids have changed the way we think about disease modeling. (illinois.edu)
I will demonstrate the functionality of our system through recordings across a range of neuronal cultures, from embryonic stem cell-derived neurons and primary brain cells to organotypic brain slices. (illinois.edu)
The morphology of ACL cells cultured on the surface of the scaffold was observed by inverted microscope. (dntb.gov.ua)
ACL cells were successfully cultured on the surface of PVA/COL scaffold, with natural growth rate, differentiation, and proliferation. (dntb.gov.ua)
We have established a highly-efficient method for deriving hepatocytes from stem cells that mirrors events in embryonic development, " said Robert Lanza, M.D., Chief Scientific Officer at Advanced Cell Technology, Inc. and senior author of the study. (news-medical.net)
In an animal model of acute liver injury, the hESC-DE cells differentiated into hepatocytes and successfully repopulated the damaged liver. (news-medical.net)
We made significant breakthrough and successfully modeled sick ARVD/C heart muscles in cell cultures using versatile stem cells derived from ARVD/C patients' skin cells with genetic mutations in desmosomal proteins (a specific type of cell-cell junctions in hearts), e.g. plakophilin-2 (Pkp2), desmoplakin (Dsp), etc. (ca.gov)
Donor T cells may also induce GvHD due to recognition of allo-antigens on non-malignant tissues of the patient. (haematologica.org)
EBV-transformed B cells (EBV-LCL) were cultured in IMDM (Cambrex, Verviers, Belgium) with 10% Fetal Bovine Serum (FBS). (haematologica.org)
We also collected macrophages and CD8 + T cells from the peripheral blood of patients with IDD for co-cultures with NP cells. (medsci.org)
And macrophages and CD8 + T cells were harvested for apoptosis analysis by FCM after 2 days of co-cultures. (medsci.org)
Schleppenbach also helped form the Nebraska Coalition for Ethical Research in 2001 to fight medical research using aborted fetal tissue and embryonic stem cells. (catholicvoiceomaha.com)
Mesenchymal stem cells (MSCs) are the most commonly used cells in tissue engineering and regenerative medicine. (frontiersin.org)
SIV antigen has been demonstrated by immunohistochemical methods in lymph node sinus histiocytes, macrophages, and giant cells (14) as well as in macrophage-derived cells in brain tissue from diseased monkeys (8). (cdc.gov)
Tissues and Cells. (lu.se)
BT-474 cells, obtained expression of the receptor has important implications for their biology from American Type Culture Collection, were maintained in RPMI 1640 and therapy (1). (lu.se)
However, post-mortem analysis of transplanted tissue revealed accumulation of pathological Lewy bodies in a small subset of transplanted cells over time, revealing a host-to-graft disease propagation. (lu.se)

To this end, in the present study, we established in vitro methods for plaque and tissue culture infectious dose 50 (TCID 50 ) assays and real-time quantitative reverse transcription (qRT)-PCR to evaluate the antiviral effects of a drug on COVID-19. (apub.kr)
For the murine PCSS, we successfully established an executable operating procedure at the methodological level to reliably generate viable and functional murine PCSS and developed new state-of-the-art analytical methods (such as LFSM 3D imaging and qRT-PCR) to increase the diversity of objective tools to evaluate PCSS. (lu.se)

In the present study, we examine the specific impacts of MSCs on mitochondrial morphology and function in host tissues. (frontiersin.org)
MSC co-culture with skin fibroblasts from mitochondrial disease patients rescued aberrant mitochondrial morphology from a fission state to a more fused appearance indicating an effect of MSC co-culture on host cell mitochondrial network formation. (frontiersin.org)

Transplantations of fetal tissue in the 1980s and 1990s provided proof-of-concept for the potential of cell replacement therapy for PD and some patients benefitted greatly from their transplants. (lu.se)

It is a thin loose covering of keratinizing skin with associated underlying eccrine (sweat) and sebaceous glands and a highly vascular stroma without underlying adipose tissue. (medscape.com)

Herpes simplex virus thymidine kinase (HSV-tk) is a well-established suicide gene that has been successfully used to control GvHD following donor lymphocyte infusions (DLI) after allogeneic stem cell transplantation. (haematologica.org)
Strains of SIV have been successfully cultured in human lymphocyte cell lines (e.g. (cdc.gov)
SIV appears to be primarily tropic for CD4 (T4)-positive leukocytes and has not been successfully propagated in B-lymphocyte cell lines (e.g. (cdc.gov)

The generation of cancer organoids from primary patient material enables a range of therapeutic agents to be tested in the resulting organoid cultures. (nature.com)

Whereas these strains replicate with WT-like kinetics in tissue culture, in vivo infections reveal a strong correlation between mutation frequency and fitness. (hal.science)
C.04.123 Poliomyelitis vaccine shall be prepared in acceptable tissue culture medium from strains of poliomyelitis virus proven capable of producing vaccine of acceptable potency. (gc.ca)

2008) specifically regarding in vitro germination of the caryopses and plant regeneration from nodal segments of both the germinated seedlings and tissue-cultured clone plants [4]. (scirp.org)

Tissue culture techniques have successfully resulted in shoot proliferation, but we have been unable to induce root initiation in explants to date. (aff.org.au)
CR-MEECs maintained a rapid and stable proliferation in this co-culture system. (biomedcentral.com)
They did this by culture organoids with nine different growth supplements (nutrients and other molecules that encourage cell proliferation). (techexplorist.com)

The protocol provided may serve as a reference to successfully establish organoids from other cancer types and perform drug screenings thereof. (nature.com)
Fig. 1: Isolation of oral mucosa organoids form primary tissue. (nature.com)
Fig. 2: Establishment of oral mucosa organoids from primary tissue. (nature.com)
Researchers successfully developed intestinal organoids from a Megachiroptera, Rousettus leschenaultia, and established a long-term stable culture method for the first time. (techexplorist.com)
The rousette bat intestinal organoids showed the same cellular composition as intestinal tissues from morphological and immunohistochemical characterizing analyses, as demonstrated previously in bats and humans. (techexplorist.com)
Together, these two techniques told the researchers that the organoids were recreating the typical cellular components of rousette bat intestine tissues. (techexplorist.com)
Human organoids are small, self-organized, three-dimensional (3D) tissue cultures that have started to revolutionize medical science in terms of understanding disease, testing pharmacologically active compounds, and offering novel ways to treat disease. (mdpi.com)

We focus on controlling morphological and histochemical responses of the shoot apical meristem (SAM) and root apical meristem (RAM) of bamboo node by using a simple and versatile liquid culture system. (scirp.org)

We have developed a 3D (spheroid) functional assay to assess the sensitivity of two PARPis (niraparib and olaparib) in ascites-derived primary cell cultures (AsPCs) from HGSOC patients. (biomedcentral.com)
HuT 78, HT, CEMx174) and in primary human and nonhuman primate peripheral blood leukocyte cultures (13). (cdc.gov)

This discussion will include steps toward co-culturing neurons and vascular cell types, functionally vascularizing the brain in vitro , and driving development of a more in vivo -like immune component. (illinois.edu)
In vivo experiments confirmed mitochondrial abundance and mitochondrial oxygen consumption rates were elevated in host tissues following MSC treatment. (frontiersin.org)
For PCSS, we developed protocols to produce viable slices of controled thicknesses which retained the ability to secrete functional α-amylase for at least two days in ex vivo culture. (lu.se)

The project has successfully established an ex situ collection of this critically endangered species at the RTBG. (aff.org.au)
SeedSafe established the Tasmanian Seed Conservation Centre at the RTBG and aims to collect and conserve 800 plant species native to Tasmania, and collect and conserve at least 60% of Tasmania's threatened flora by 2010. (tas.gov.au)

Mycetoma is a chronic, progressively destructive inflammatory disease of the skin, subcutaneous and connective tissue, muscle and bone. (who.int)
Organomegaly, connective-tissue and ocular pathology, and central nervous system dysfunction may result. (medscape.com)

Additionally, it will explain the techniques of collecting, packaging, and transporting bone marrow aspirates for collection, expansion, and 150 injections into diseased tissues. (ivis.org)
She was awarded with a postdoc fellow from the Ministry of Science at the Technical University of Catalonia in Biomaterials for bone tissue regeneration and since 2010 has been professor at this university. (ibecbarcelona.eu)
Nonpulmonary soft-tissue densities caused by nipple shadows, warts, cutaneous nodules, and bone abnormalities are often confused for a nodule on chest x-ray. (msdmanuals.com)

The key hurdle was to multiply tissue culture plants in beds of growing media and maintain the physiological young stage of the mother plants from which apical cuttings could be repeatedly taken for several months to produce ARC for sale to farmers who demanded the new cultivars (cvs) with all the desirable attributes. (intechopen.com)
He highlighted the developments achieved in preservation of exotic, primitive herbal plants in National Herbarium, rearing of animals for improved dairy products at Animal Sciences Institute, tissue culture advancement for the acquisition of disease free banana and potato plants at National Institute for Genomic and Advanced Bio-Technology (NIGAB) and intensive fish farming. (com.pk)
However, in overall observation in this study, Granola showed better performance from meristem tissue culture and all plants were found normal and free from potato viruses that were tested through ELISA test. (ejfood.org)
Schefflera Alpine Wholesale Plantlets Tissue Culture Young Plants is a good example to showcase our research and development capability. (youngplant.cn)
ICGV-90704 and Chalimbana performed better than the other three varieties for shoot organogenesis although all varieties produced healthy rooted plants in vitro that were successfully transferred to the greenhouse where they exhibited normal growth, flowering and seed set. (ac.ke)

The demystification of tissue culture by the 1980s greatly aided this development. (intechopen.com)

DNA damage response analysis, soft agar assay, and matrigel 3D culture were carried out to determine the normal biological characteristics of CR-MEECs. (biomedcentral.com)
The tissue-specific differentiation potential of MEECs was analyzed with air-liquid interface (ALI) 3D culture, hematoxylin and eosin (H&E) staining, Masson's trichrome and DAB staining, immunofluorescence assay. (biomedcentral.com)
We optimized an assay for testing antiviral activity by plaque reduction, tissue culture infectious dose 50, and quantitative RT-PCR. (apub.kr)

Our aim was to establish a live cell culture to enable the understanding of the cellular processes driving the non-malignant growth of these tumors. (frontiersin.org)

In the present work, we cultured different tissues of B. tenagophila, the second most important host of S. mansoni in Brazil, using a strain that is naturally and absolutely resistant to S. mansoni infection. (fiocruz.br)
The orbital infection was treated successfully with long-term oral clarithromycin. (houstonmethodist.org)
Furthermore, whereas WT virus is readily detectable in target organs 30 d after infection, some variants fail to successfully establish persistent infections. (hal.science)
The disease commonly affects young adults, particularly men aged between 20 and 40 years.2 Infection is thought to be acquired by traumatic inoculation of fungi or bacteria into the subcutaneous tissue following minor trauma or a penetrating injury, commonly thorn pricks. (who.int)

State and local public health authorities, other infectious disease experts, and CDC will reexamine currently reportable diseases, establish criteria for making a disease reportable, and explore ways to enhance rapid reporting of cases from clinical laboratories and health care practitioners. (cdc.gov)

It was the use of PCR that for leishmaniasis before and was treated al owed us to establish the diagnosis similarly with systemic meglumine anti- and identify the parasite responsible for moniate (Glucantime, Aventis, France) the lesions as L. major . (who.int)

As the species is critically endangered a number of propagation techniques were attempted, including seed, tissue culturing and standard vegetation propagation, to establish an ex situ population. (aff.org.au)
the seed has been successfully harvested and banked), 18 are still active and 5 are in propagation. (tas.gov.au)
A method for AsPCs preparation was established based on a matrix (agarose), allowing for easy isolation and successive propagation of monolayer and 3D AsPCs. (biomedcentral.com)
In vitro culture for propagation and hybridization through conventional breeding may also trigger its activation. (nature.com)

However, to date, no model has successfully incorporated all major brain tissue components together with a functional blood-brain barrier. (illinois.edu)

8 nm).Ppy NPs effectively augment the conductivity, surface roughness, andthickness of BC composites despite reducing scaffolds' transparency.BC-Ppy composites were flexible (up to 10 mM Ppy), maintained theirintricate 3D extracellular matrix-like mesh structure in all Ppy concentrationstested, and displayed electrical conductivities in the range of nativecardiac tissue. (ibecbarcelona.eu)
Our results highlight the remarkablepotential use of BC-Ppy scaffolds as a cardiac patch in tissue regenerativetherapies. (ibecbarcelona.eu)
Background and Purpose: Suitable tissue-engineered scaffolds to replace human anterior cruciate ligament (ACL) are well developed clinically as the development of tissue engineering. (dntb.gov.ua)
Conclusion: The model of in-vitro tissue-engineered ACL was successfully established by PVA/COL scaffolds. (dntb.gov.ua)

Achieving integrated and coupled neuronal, vascular, and immune components within a unified brain-on-chip platform would establish a powerful system to better represent the complex brain microenvironment and more closely emulate the brain in healthy and pathological states. (illinois.edu)

As with other tissues, there is a scarcity of donor livers and hepatocytes, which is compounded by the low recovery and proliferative capacity of adult hepatocytes. (news-medical.net)

Remdesivir reduced SARS-CoV-2 titer without detectable cytotoxicity and successfully inhibited viral replication in a dose-dependent manner. (apub.kr)

Immunohistochemistry was performed in both tissue and 3D sections for markers including synaptophysin, vimentin, neurofilament and MIB-1. (frontiersin.org)
Using immunohistochemistry, the 3D cultures were shown to express markers as in the original DIG tumor tissue indicating that the spheroid cultures were able to maintain the heterogeneity found in the original tumor. (frontiersin.org)
Studied the temporal expression of RB1 mRNA in wild type mice to better understand the role of RB1 in dental stem cell differentiation using immunohistochemistry on formalin fixed and frozen tissue sections. (livecareer.com)

Review of the literature on ocular/adnexal and soft tissue infections caused by atypical mycobacteria shows characteristic clinical and histopathologic features. (houstonmethodist.org)

C.04.130 No fabricator shall permit the introduction of any bacterial or viral cultures other than those used in the manufacture of poliovirus vaccine, live, oral on any premises that are used for the manufacture of poliovirus vaccine, live, oral. (gc.ca)

Our findings from clinical maternal cultures are reassuring, but ongoing monitoring for changes in resistance profiles is needed," they write. (medscape.com)

Figure 3).The patient complained nia amastigote DNA in tissue samples of recurrence and progression of his obtained from patients with chronic disease. (who.int)

The patient reported in the current study illustrates the difficulty in establishing the preoperative diagnosis of atypical mycobacterial infections of the orbit. (houstonmethodist.org)

Our data confirmed that CR-MEECs retained normal biological characteristics and endometrium tissue-specific differentiation potential. (biomedcentral.com)
The differentiation process was largely uniform with cell cultures progressively expressing increasing numbers of hepatic lineage markers. (news-medical.net)

Most importantly, allogeneic transplantation of CR-MEECs successfully repaired the injured endometrium and significantly improved the pregnancy rate of IUA mice. (biomedcentral.com)

Microfluidic organ-on-chip (OoC) systems recapitulate the human physiology by advanced 3-dimensional (3D) cell culture, but lack in parallelization and automation and are consequently barely used for pharmakinetic analysis or high-throughput screening. (utwente.nl)
At the same time, existing parallelized microfluidic cell culture platforms enable automated control over the cell culture conditions, but are limited to straightforward 2D cell culture due to the use photolithography based wafer molds. (utwente.nl)
The larger and more complex 3D cell culture compartments can be rapidly prototyped by micro-milling, which enables fast and easy redesign of the microfluidic chip. (utwente.nl)
This facilitates tight and spatiotemporal control over each of the individual cell culture compartments. (utwente.nl)
It was demonstrated that cell culture chambers of 0.9 µl were filled with a f low rate of 2.7 µl/s. (utwente.nl)
It was observed that the success rate was depending on the cell culture density. (utwente.nl)
The cell tropism of SIV in culture depends partially on the strain of virus propagated and conditions of cell culture. (cdc.gov)

Performed a forward ENU screen on zebrefish and successfully identified a phenotype mutant with mineralization defect which could be eventually translated into human models for craniofacial, dental or skeletal disorders. (livecareer.com)
These in vitro systems broaden our ability to understand human biology by addressing the critical lack of access to functional human tissue. (illinois.edu)

We first successfully established long-term cultures of MEECs using CR approach. (biomedcentral.com)
More than 95% of this activity is inhibited in assays where substrate has been added to a preincubated mixture of tissue with the non-neuropathic organophosphorus compound (OP) paraoxon (O,O'-diethyl p-nitrophenyl phosphate): residual activity includes soluble neuropathy target esterase (S-NTE) which, by definition, is considered resistant to long-term progressive (covalent) inhibition by paraoxon. (inrae.fr)

Our professionals would love to help you at any time.Foshan Sanshui Youngplants Co., Ltd. has established stable business relationship and service networks in many countries. (youngplant.cn)

2) Since the obtained shoots of a 1-month-old culture box showed growth variation, we distinguished shoot types based on plant height, i . e ., short (less than 5 cm), medium (ca. 5 - 10 cm), and tall (more than 10 cm). (scirp.org)
S. S. Bhojwani and M. K. Razdan, "Plant tissue Culture: Theory and practice," Elsevier Scientific Publishing Co. Amsterdam, p. 520, 1983. (ejfood.org)
The part of the stem that is covered with soil will often develop roots and can be separated from the mother plant once it has established. (pc-mobile.net)

The technology was first developed in warmer climates at lower elevations of less than 1,500 meters above mean sea level (mamsl) but gradually it was successfully developed at cooler climates in East Africa. (intechopen.com)
Rooted plantlets were gradually acclimatized and successfully established in the field. (ejfood.org)

Based on these novel findings, we identified and tested two potential clinically safe drugs in their efficacy of treating an established ARVD/C mouse model. (ca.gov)

The SPD model was subsequently incorporated into our previously-established cell cycle model, forming a comprehensive, multi-scale quantification platform for evaluating drug effects across multiple scales, spanning the proteomic-, cellular-, and subcellular levels. (bvsalud.org)

Both sources have low morbidity associated with tissue harvest, and they are not associated with detrimental effects on donor sites of collection because of the renewable nature of these tissues. (ivis.org)

5) The detailed autofluorescence properties of the outer regions of culm and node tissues were also identified using an inverted fluorescent microscope under B- and U-excitation lights with RGB and HSB (hue, saturation, and brightness) digital imaging analysis. (scirp.org)

When Kerala Agricultural University was established in 1972, Banana Research Station, Kannara was taken over by KAU. (wikipedia.org)
This station successfully developed tissue culture banana farming in 2011. (wikipedia.org)

Second, they used immunofluorescence staining-a common method used to detect and visualize molecules in biological samples-to look for molecular markers that indicate that the tissue under investigation comes from bat intestines. (techexplorist.com)
C.04.134 A fabricator of poliovirus vaccine, live, oral shall test, by a method acceptable to the Minister, each lot of vaccine for neurovirulence and for genetic markers and it shall meet the requirements established by the Minister. (gc.ca)

Thus, tissue-derived cultures of Biomphalaria may be useful in increasing the understanding of that interaction at cellular level. (fiocruz.br)
Herein, we will discuss current techniques and advances moving us closer to realizing this multi-cellular integrated brain co-culture system. (illinois.edu)

We now show that, surprisingly, removal of paraoxon by ultrafiltration ('P' tissue) in order to avoid such an interference results in the reappearance of about 65% of total original soluble PVase activity which is inhibited in the presence of this OP. Although a purely reversible non-progressive inhibition might be suspected, kinetic analysis data show a time-progressive inhibition which suggests that such PVase(s) covalently bind paraoxon. (inrae.fr)
Our findings demonstrate that real-time PCR assays targeting 100-kDa and H antigen showed the most reliable results and can be successfully used for diagnosing this mycosis when testing FFPE samples. (cdc.gov)
The importance of tracking outbreaks at a global scale has raised the urgency of establishing high-accuracy pipelines that generate consistent results between research groups. (cdc.gov)

4) By culturing the first node in the SLCE system, we performed a quick survey during the 3 weeks in the culture and found that a combination of 10 μM benzyl adenine and 3 μM thidiazuron was effective for in vitro SAM development, while the addition of 2, 4-D was effective for promoting in vitro RAM development. (scirp.org)
The in-house R&D facilities located at Ranga Reddy District in Telangana are recognized by the Department of Science & Technology Government of India and also established its state-of-the-art biotech laboratory with facilities for tissue culture transgenic crop development molecular biology molecular breeding radio isotope laboratory molecular QC laboratory Insectory and large containment green houses. (business-standard.com)
In this regard, he informed the Committee that an excellent working relationship had been established as part of our common development objectives and specific strategies to deal with the major challenges in the African Region. (who.int)

To test the antiviral activity of a drug against COVID-19, in this study, we established and compared experimental conditions, such as the treatment time and mode of action (dose) of the therapeutic substance, and a test method to evaluate its effectiveness. (apub.kr)

A tissue culture protocol of bamboo (Phyllostachys meyeri McClure, Pm) was successfully achieved by Ogita et al. (scirp.org)
Successfully upscaled the protocol for multiple formulations in order to transfer it to the production department. (livecareer.com)
A wide range of push-down valve geometries were characterized, which showed that the quality highly depends on the height and width of the channel, and on the thickness and elasticity of the membrane fabricated by a well- established protocol. (utwente.nl)
The experiments were conducted with a view to establish a protocol for virus free mini-tubers production, in vitro, using potato meristem as explant, collected from potato sprouts of three popular potato varieties Diamant, Cardinal, Granola. (ejfood.org)

3) Three types of node portions-the first node (the base node near a rhizome tissue), middle nodes (upper nodes near the 1 st node), and the top meristem-were independently cultured in the SLCE, and it was found that the first node showed the best growth performance. (scirp.org)
This problem could be significantly mitigated by coordinately screening multiple tissue types simultaneously in a high-throughput fashion during upstream tier 1 screening. (inknowvation.com)

The aim of this study was to explore the feasibility of constructing tissue-engineered ACL by the copolymerization of PVA and collagen (PVA/COL). (dntb.gov.ua)
This study established a basis for genetic engineering activities on African groundnuts in the future. (ac.ke)
However, the study did not collect the culture data necessary to confirm this, they write. (medscape.com)

The implications of these findings in the definition of NTE in a target tissue for the so-called organophosphorus-induced delayed polyneuropathy (OPIDP) are discussed. (inrae.fr)
A thorough physical examination may uncover findings that suggest an etiology (eg, a breast lump or skin lesion suggestive of cancer) for a pulmonary nodule but cannot definitely establish the cause. (msdmanuals.com)

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