• In contrast to RP-LC, gradient elution HILIC begins with a low-polarity organic solvent and elutes polar analytes by increasing the polar aqueous content [ 13 ]. (springer.com)
  • Separation was performed on a Phenomenex® Kinetex C18 column (100 Å × 3.5 µm × 4.6 mm × 150 mm) using a gradient elution program with a mobile phase of 10 mM ammonium formate-methanol. (bvsalud.org)
  • 5 μm) and a mobile phase consisting of mixture acetonitrile: 0.1% phosphoric acid in gradient elution. (gov.vn)
  • Chromatographic separation was achieved on a Polaris 3 C18-A column using gradient elution with 0.2% (v/v) formic acid and 0.2% (v/v) ammonium hydroxide in water (A) and methanol (B) as eluents. (uu.nl)
  • mm) under isocratic elution of 0.75. (okstate.edu)
  • The separation was achieved on a reversed phase C(18) column (Luna C(18)), 5 microm by isocratic elution with 0.05 M sodium sulphate buffer (pH 2.3) and acetonitrile (68.5 : 31.5, v/v) as the mobile phase at a flow rate of 1.0 ml/min with an operating temperature of 30 degrees C. The method was validated for linearity, precision, intra- and inter-day precision and accuracy. (lookformedical.com)
  • Despite the absence of an organic solvent in the mobile phase, six Gd-based contrast agents, Gd-DTPA, Gd-EOB-DTPA, Gd-DOTA, Gd-DTPA-BMA, Gd-BT-DO3A, and Gd-HP-DO3A, were successfully separated during an isocratic elution lasting 13 min. (speciation.net)
  • The entire chromatographic analysis was performed with isocratic elution of the composition of the mobile phase (acetonitrile:water, 60:40, v:v, 0.032% glacial acetic acid) on the C18 column. (bezmialem.edu.tr)
  • Isocratic elution was employed using a mixture of methanol, acetonitrile and a 0.28% (v/v) of acetic acid (5:10:35). (najah.edu)
  • The separation was carried out by isocratic elution through C18 column (4.6 mm × 250 mm i.d., 5 μ particle size) at 40 °C temperature at a constant flow rate of 1.0 mL/min using acetonitrile and 1% orthophosphoric acid as buffer (20:80, v/v). A 10 μL sample was injected and elution was monitored at 210 nm wavelength. (asianjournalofchemistry.co.in)
  • The co-elution control sample contained 5 mM of the test substance in phosphate buffer solution. (europa.eu)
  • The co-elution control sample contained 25 mM of the test substance in ammonium acetate buffer solution. (europa.eu)
  • Adding elution buffer, and removing the magnetic field then releases the DNA as a purified sample, ready for quantitation and further analysis, including reverse transciption (for RNA), QRT-PCR and next generation sequencing applications. (avicorbiotech.com)
  • The unique vacuum plate design provides zero buffer retention and low elution volumes. (zymoresearch.eu)
  • Q1: What is the composition of the ZymoPURE™ Elution buffer? (zymoresearch.eu)
  • To increase elution of large plasmid DNA, we recommend pre-warming the ZymoPURE Elution Buffer (50 ºC) and increasing the incubation time on column up to 10 minutes prior to centrifugation. (zymoresearch.eu)
  • A new stability-indicating method was established for the estimation of Rifaximin by RP-HPLC The chromatographic conditions were successfully developed for the estimation of Rifaximin by using symmetryC18 column (150×4.6mm,5μ), flow rate of 1ml/min, mobile phase ratio used was (65:35 v/v) methanol: phosphate buffer pH 3 (pH was adjusted with orthophosphoric acid),detection was carried at 454nm. (ijcpa.in)
  • but modified the protocol by increasing the initial wastewater volume to 90 mL (2 tubes of 45 mL) and eluting all the extracted nucleic acids in 50 µL of elution buffer supplied with the kit. (cdc.gov)
  • The following parameters that may affect the solid-phase extraction procedure efficiency were optimized: different elution solvents and the pH value of pesticide solution. (ac.rs)
  • An additional control sample of 5 mM of test substance in acetonitrile was also prepared so as to positively identify peak(s) of the test item in the co-elution control ensuring that the test item had not evaporated away during incubation and injection of the samples on the HPLC. (europa.eu)
  • Analysis for acrolein is accomplished by elution on a reverse phase column using high performance liquid chromatography (HPLC), and detection of the adduct by ultraviolet (UV) absorbency. (cdc.gov)
  • A direct recovery of papain enzyme from unclarified Carica papaya juice was developed successfully using a reversed phase expanded bed adsorption chromatography (RP-EBAC). (globalstf.org)
  • Reagent kit for the elution of microorganisms from a membrane filter after enrichment by vaccum. (sysmex-flowcytometry.com)
  • The appearance of the substance, positive control samples and co-elution controls in the HPLC vials was documented following preparation with the vials then placed into the autosampler of the HPLC set at 25°C for a minimum of 22 hours incubation prior to initiation of the analysis run. (europa.eu)
  • A rapid, accurate, precise and sensitive HPLC method with ultraviolet (UV) detection was developed, optimised and validated for the in-vitro analysis of EFV in a total run time under 10 minutes for the elution of both EFV and loratidine which was used as the internal standard (IS). (ru.ac.za)
  • The proposed method was successfully applied for quantitative determination of phytomelin in leaves, fruits, and flowers of Ecballium elaterium (L.) A. Rich Keywords: Exploding cucumber, Ecballium elaterium, HPLC analysis, glycoside, Phytomelin content. (najah.edu)
  • Although dsDNA has the same length as its single-stranded counterpart, the dsDNA elutes at lower elution volumes when separated by SEC. This behaviour is most probably due to the larger hydrodynamic radius of dsDNA compared to ssDNA, which results in faster diffusion through the stationary phase. (chromatographytoday.com)
  • Hence, the focus of our study was to select a mobile AF4 phase required to successfully separate and quantify AgNP in soil water extracts. (wur.nl)
  • The presence of clay colloids in the soil water extracts dramatically increased the elution time of PVP-coated AgNP, suggesting an interaction between these AgNP and clay minerals. (wur.nl)
  • Several Bacopa samples (plant materials, extracts and commercial formulations) were successfully analyzed. (lookformedical.com)
  • Extraction efficiencies of MMMs for ATSs were evaluated by studying the effects of membrane matrix, pH, elution reagents, shaking time, and dissolved organic content. (chromatographyonline.com)
  • In this realm, the normal mode elution order of smaller analytes eluting prior to larger ones was shown to be invertible, exemplified by the elution of a 40 kDa analyte prior to IFN-α2a. (uni-muenchen.de)
  • Avicor Ltd. successfully developed several new products in the frame of a Hungarian R&D grant (KFI_16-1-2017-0105) for specific enrichment of nucleic acids deriving from diverse biological samples specific for human, bacterial, fungal and viral nucleic acids. (avicorbiotech.com)
  • Complete elution (100% efficiency) of NO3−N from the binding phase was achieved using a solution of 5% NaCl. (lancs.ac.uk)
  • As the drug peak elution did not interfere with any degradants during the forced degradation studies and therefore the proposed method can be successfully applied to perform long-term and acceleratedstability studies of Rifaximin formulation. (ijcpa.in)
  • You have successfully reset your password. (promega.com)
  • The gradual elution of lanthanides for the separation of 171 Tm. (psi.ch)
  • Due to the AF4 feature to discretionarily alter the resolution power within one separation run, the fractionation of higherorder aggregates and insoluble, precipitated protein was successfully performed. (uni-muenchen.de)
  • The developed procedure was successfully applied to a study aimed at evaluating the pharmacokinetic profiles of febuxostat and montelukast in human plasma. (bezmialem.edu.tr)
  • The use of SDS as a mobile AF4 phase holds promise to successfully characterize AgNP in soil pore water. (wur.nl)
  • Figure 3: Comparison of the elution volume of ssDNA and dsDNA by using YMC-Pack Diol columns with different pore sizes. (chromatographytoday.com)
  • In SEC the choice of an optimal pore size is crucial to successfully analysing oligonucleotides of different lengths. (chromatographytoday.com)
  • Interferences due to the coincidental elution of DNP adducts of ketones or aldehydes other than acrolein are not ruled out by this method of analysis. (cdc.gov)
  • The developed method was successfully applied to ongoing in vivo vinblastine pharmacokinetic studies in dogs. (okstate.edu)
  • The method was successfully applied in the analysis of 17 samples in the local market. (gov.vn)
  • This method has been tested successfully in grouping the bones, clotted blood and blood-stains on cloth, weapons and other articles [ 4 ]. (rroij.com)
  • The method was successfully applied to a preclinical study with cintirorgon in mice. (uu.nl)
  • The method was then successfully applied to the determination of EFV in commercially available tablets. (ru.ac.za)
  • Obtained results indicate that carbonized and activated hemp fibers could be successfully applied as a solid-phase sorbent for the pesticide analysis in water samples. (ac.rs)
  • GAPDH, NRXN3 and ZBTB18 amplified successfully in all samples, but NTRK3 did not. (biomedcentral.com)
  • Blood group activities of the tooth fragments, which were detected with absorption-elution test, were markedly decreased when the odontoblastic zone was scraped off. (go.jp)
  • Concomitantly, substantial progress was made in pharmaceutical formulation development of peptides and proteins, inasmuch as many challenges in formulating these compounds in products with optimal therapeutic effects and shelf life were successfully approached. (uni-muenchen.de)
  • Carbon columns, chemical mixing tanks and elution circuit platforms are now in place and connection of facilities is now underway. (steppegold.com)
  • Yes, the standard ZymoPURE protocol has been successfully tested with constructs up to 200 kb. (zymoresearch.eu)
  • Dr. Geiger reported that 242 million Ebola viruses were captured within the Hemopurifier® during treatment, a number verified by a post-treatment elution protocol. (pharmaceuticalprocessingworld.com)
  • The NO3−N ion selectivity of the SIR-100-HP/ agarose binding layer was retained in the presence of high multivalent ion concentrations and was used successfully to acquire in situ NO3−N measurements in bulk soil. (lancs.ac.uk)
  • CMC lead for the development of Amgen's first MAb, delivering successfully a clinical manufacturing process for purified bulk within an accelerated timeline. (lagunasource.com)
  • In order to separate this nuclide from the carbon matrix, pyrolysis at 1000 °C in an oxygen stream has been successfully applied to extract ppm quantities (4 mg) of 10 Be from 270 g of carbon, see Figure below. (psi.ch)
  • Group specific colorization was successfully developed on the cell surface of the odontoblasts. (go.jp)
  • Five mimotope peptides were obtained by panning-elution from the library and were successfully used in an indirect competitive ELISA for analyzing total aflatoxin concentration. (cdc.gov)
  • Two high-performance Cu catalysts were successfully developed by immobilization of Cu ions in the nanospaces of poly(propylene imine) (PPI) dendrimer and magadiite for the selective C-C coupling of 2,6-dimethylphenol (DMP) to 3,3',5,5'-tetramethyldiphenoquinone (DPQ) with O 2 as a green oxidant. (mdpi.com)
  • Two-step elution was employed to enhance the purity of papain in RP-EBAC which gives high papain purity of 74.98% and high purification factor of 7.04. (globalstf.org)
  • Aethlon previously reported that Hemopurifier® therapy was successfully administered to a critically-ill Ebola patient at Frankfurt University Hospital in Germany. (pharmaceuticalprocessingworld.com)