AnatomyOrganismsChemicals and DrugsAnalytical, Diagnostic and Therapeutic Techniques and EquipmentPhenomena and ProcessesInformation Science
Protein Kinase CProtein KinasesCyclic AMP-Dependent Protein KinasesCalcium-Calmodulin-Dependent Protein KinasesProtein-Serine-Threonine KinasesMitogen-Activated Protein KinasesPhosphorylationProtein Kinase InhibitorsProtein SubunitsMAP Kinase Signaling Systemp38 Mitogen-Activated Protein KinasesPhosphatidylinositol 3-KinasesEnzyme ActivationMitogen-Activated Protein Kinase 1Protein Kinase C-alphaMitogen-Activated Protein Kinase KinasesProtein Kinase C-deltaMitogen-Activated Protein Kinase 3Signal TransductionMolecular Sequence DataJNK Mitogen-Activated Protein KinasesAMP-Activated Protein KinasesAmino Acid SequenceIsoenzymesProtein Kinase C-epsilonProtein Kinase C betasrc-Family KinasesTetradecanoylphorbol AcetateCyclic GMP-Dependent Protein KinasesKineticsProtein-Tyrosine KinasesCell LineEnzyme InhibitorsCDC2 Protein KinaseCyclic AMPCasein Kinase IIProtein BindingCells, CulturedCalcium-Calmodulin-Dependent Protein Kinase Type 2MAP Kinase Kinase KinasesMutationBase SequenceTransfectioneIF-2 KinaseBlotting, WesternCalciump21-Activated KinasesSubstrate SpecificityMAP Kinase Kinase 1Recombinant ProteinsSerineBinding SitesExtracellular Signal-Regulated MAP KinasesMacromolecular SubstancesRibosomal Protein S6 KinasesPhosphoproteinsProto-Oncogene Proteins c-aktCasein KinasesRNA, MessengerProto-Oncogene ProteinsCyclin-Dependent KinasesMAP Kinase Kinase 4Sequence Homology, Amino AcidProtein Structure, TertiaryPhorbol 12,13-DibutyrateDiglyceridesIntracellular Signaling Peptides and ProteinsRecombinant Fusion ProteinsMaleimidesIsoquinolinesStaurosporineMolecular Weight1-(5-Isoquinolinesulfonyl)-2-MethylpiperazinePhorbol EstersCloning, MolecularElectrophoresis, Polyacrylamide GelCreatine KinaseCyclic AMP-Dependent Protein Kinase Type IIDNA-Activated Protein KinaseIndolesGlycogen Synthase Kinase 3Phosphotransferases (Alcohol Group Acceptor)Tumor Cells, CulturedPhosphoprotein PhosphatasesApoptosisPyruvate KinaseGene Expression Regulation, Enzymologic3-Phosphoinositide-Dependent Protein KinasesImmunoblottingTyrosineReceptor Protein-Tyrosine KinasesDose-Response Relationship, DrugFlavonoidsPrecipitin TestsAdenosine TriphosphateRats, Sprague-DawleyCell MembraneCattleThreonineGene Expression Regulation
PhosphorylationCAMP-dependRegulatoryPhosphatidylinositol 3-kinaGeneInhibitorAMPKMutationsInhibitoryGenesOligomeric proteinInhibitorsMonoclonalEnzymeCaMKIIMRNAAmino acidsIntracellularUbiquitous proteinAntibodyMolecularAtaxiaMultiproteinHumanAutophosphorylationSynapticReplicationRole in the regulationNeuronsDifferentiationActivityRegulate cellNuclearActivationSynthesisComplex
Phosphorylation13
  • This antibody does not detect the non-phosphorylated α-subunit of rat CaM kinase II nor the ~60kDa β-subunit in either phosphorylation state. (enzolifesciences.com)
  • After treatment with imatinib in mononuclear cell (MNC) of CML patients and K562 in vitro, expression of DNA-PKcs mRNA was detected by RT-PCR and DNA-PKcs protein level, tyrosine phosphorylation of bcr-abl fusion protein were detected by Western blot. (hku.hk)
  • After treatment of MNC of CML and K562 with imatinib in vitro, the expression of DNA-PKcs protein was enhanced while tyrosine phosphorylation of bcr-abl fusion protein decreased. (hku.hk)
  • Regulates the abundance of compartmentalized pools of its regulatory subunits through phosphorylation of PJA2 which binds and ubiquitinates these subunits, leading to their subsequent proteolysis. (rcsb.org)
  • cAMP exerts its effects by activating the cAMP-dependent protein kinase, which transduces the signal through phosphorylation of different target proteins. (nih.gov)
  • This phosphorylation is regulated by the ataxia telangiectasia mutated (ATM) and the Rad3-related (ATR) kinases in response to various genotoxic stresses, making it a critical regulatory element of DNA-PKcs ( 6 , 10 ). (amegroups.org)
  • An in vitro phosphorylation assay demonstrated that PKA phosphorylated the recombinant protein of an active trans-sialidase. (elsevierpure.com)
  • We found that DNA-dependent protein kinase catalytic subunit (DNA-PKcs) and mammalian target of rapamycin (mTOR) complex 2 (mTORC2) were both required for UVB-induced Akt Ser-473 phosphorylation in keratinocytes. (biomedcentral.com)
  • Inhibition of DNA-PKcs activity via its inhibitor NU7026, a dominant-negative kinase-dead mutation, RNA interference (RNAi) or gene depletion led to the attenuation of UVB-induced Akt Ser-473 phosphorylation. (biomedcentral.com)
  • These results are consistent with the hypothesis that both binding of active PKAc to dSlo and phosphorylation of dSlo or some other protein are necessary for channel modulation. (elsevierpure.com)
  • Fig. 1: Serum mitogen-induced phosphorylation of PI3KC2-β induces complex formation with 14-3-3 proteins. (nature.com)
  • Fig. 5: mTORC2 signalling triggers PKN2 activation to control PI3KC2-β phosphorylation and sequestration with 14-3-3 proteins. (nature.com)
  • The alpha isoform is responsible for synaptic plasticity and participates in memory through its phosphorylation of synaptic proteins. (elifesciences.org)
CAMP-depend1
  • Drosophila Slowpoke (dSlo) calcium-dependent potassium channels bind directly to the catalytic subunit of cAMP-dependent protein kinase (PKAc). (elsevierpure.com)
Regulatory14
  • In molecular biology, the cyclin-dependent kinase regulatory subunit family is a family of proteins consisting of the regulatory subunits of cyclin-dependent protein kinases. (wikipedia.org)
  • The proteins bind to a regulatory subunit, cyclin-dependent kinase regulatory subunit (CKS), which is essential for their function. (wikipedia.org)
  • This regulatory subunit is a small protein of 79 to 150 residues. (wikipedia.org)
  • The regulatory subunits exist as hexamers, formed by the symmetrical assembly of 3 interlocked homodimers, creating an unusual 12-stranded beta-barrel structure. (wikipedia.org)
  • The protein encoded by this gene is a regulatory subunit of the AMP-activated protein kinase (AMPK). (ageing-map.org)
  • This subunit is one of the gamma regulatory subunits of AMPK. (ageing-map.org)
  • The inactive kinase holoenzyme is a tetramer composed of two regulatory and two catalytic subunits. (nih.gov)
  • cAMP causes the dissociation of the inactive holoenzyme into a dimer of regulatory subunits bound to four cAMP and two free monomeric catalytic subunits. (nih.gov)
  • Four different regulatory subunits and three catalytic subunits have been identified in humans. (nih.gov)
  • This gene encodes one of the regulatory subunits. (nih.gov)
  • In this study, we identify the zinc finger protein Zbtb18 as a transcriptional repressor that directly binds enhancer/promoter regions of genes encoding class I PI3K regulatory subunits, subsequently limiting their expression, dampening PI3K signaling and suppressing PC responses. (ox.ac.uk)
  • dSlo binds to free PKAc but not to the PKA holoenzyme that includes regulatory subunits and is inactive. (elsevierpure.com)
  • If independently confirmed in the future, the study will stand as having provided a novel regulatory mechanism for the autophosphorylation of this kinase. (elifesciences.org)
  • The hub connects to the N-terminal kinase domains through a variable-length linker and regulatory domain. (elifesciences.org)
Phosphatidylinositol 3-kina3
  • DNA-dependent protein kinase catalytic subunit (DNA-PKcs), a member of the phosphatidylinositol 3-kinase related kinase family is a well-known player in repairing DNA double strand break through non-homologous end joining pathway. (molcells.org)
  • DNA-dependent protein kinase catalytic subunit (DNA-PKcs), encoded by PRKDC gene, is a member of the phosphatidylinositol 3-kinase-related kinase (PIKK) family, which also includes the two DNA damage repair proteins, ataxia telangiectasia mutated (ATM) and ATM and Rad3-related protein (ATR). (molcells.org)
  • The Zinc Finger Protein Zbtb18 Represses Expression of Class I Phosphatidylinositol 3-Kinase Subunits and Inhibits Plasma Cell Differentiation. (ox.ac.uk)
Gene7
  • A single nucleotide polymorphism (SNP) of the protein kinase catalytic subunit alpha-1 gene ( PRKAA1 ) that confers susceptibility to gastric cancer (GC) was identified by genome-wide association in several case-control studies. (oncotarget.com)
  • The protein encoded by this gene is highly similar to Cdc 37, a cell division cycle control protein of Sacchromyces cerevisiae. (nih.gov)
  • Menin protein, produced by the MENIN gene, is a tumor suppressor. (medscape.com)
  • This gene provides instructions for making one part (subunit) of an enzyme called protein kinase A, which promotes cell growth and division (proliferation). (medlineplus.gov)
  • The subunit produced from the PRKAR1A gene, called type 1 alpha, helps control whether protein kinase A is turned on or off. (medlineplus.gov)
  • Most mutations in the PRKAR1A gene that cause Carney complex result in an abnormal type 1 alpha subunit that is quickly broken down (degraded) by the cell. (medlineplus.gov)
  • Finally, it seems appropriate to consider the "sodium channel syndrome" (mutations in the gene of the α subunit of the sodium channel, SCN5A gene) as a single clinical entity that may manifest in a wide range of phenotypes, to thus have a better insight on these cardiac syndromes and potential outcomes for their clinical treatment. (bvsalud.org)
Inhibitor4
  • A screen profiling 158 kinase inhibitors (Calbiochem Protein Kinase Inhibitor Library I and II, catalogue numbers 539744 and 539745) for their inhibitory activity at 1µM and 10µM against 234 human recombinant kinases using the EMD Millipore KinaseProfiler TM service. (guidetopharmacology.org)
  • Inhibition of DNA-PKcs activity via a small interfering RNA or a kinase inhibitor results in mitosis delay, abnormal spindle formation, and chromosome misalignment. (amegroups.org)
  • Small-molecule inhibitor targeting the Hsp90-Cdc37 protein-protein interaction in colorectal cancer. (nih.gov)
  • Inhibition of DNA-dependent protein kinase catalytic subunit by small molecule inhibitor NU7026 sensitizes human leukemic K562 cells to benzene metabolite-induced apoptosis. (cdc.gov)
AMPK6
  • 3. Littler DR, Walker JR, Davis T, Wybenga-Groot LE, Finerty PJ, Newman E, Mackenzie F, Dhe-Paganon S. (2010) A conserved mechanism of autoinhibition for the AMPK kinase domain: ATP-binding site and catalytic loop refolding as a means of regulation. (guidetopharmacology.org)
  • AMPK subfamily: protein kinase AMP-activated catalytic subunit alpha 2. (guidetopharmacology.org)
  • PRKAA1 (Protein kinase AMP-activated catalytic subunit alpha 1), also known as AMPK α1, is an energy sensor that plays a key role in regulating cell energy metabolism. (bocsci.com)
  • AMPK α1 is a catalytic subunit of heterotrimeric AMPK protein with a length of 548 amino acids. (bocsci.com)
  • AMPK proteins are dysfunctional in several human diseases, including diabetes and metabolic syndrome, cardiovascular disease, neurodegenerative diseases and many types of cancer. (bocsci.com)
  • AMPK is a heterotrimer consisting of an alpha catalytic subunit, and non-catalytic beta and gamma subunits. (ageing-map.org)
Mutations4
  • Autosomal dominant mutations in one of the most ubiquitous isoforms of the inhibitory subunit (R1α) of protein kinase A (PKA) are linked to a generalized tumor predisposition, referred to as Carney complex (CNC). (mcmaster.ca)
  • NEMO consists of 10 exons, and most mutations cause deletions of exons 4-10, resulting in a truncated protein. (medscape.com)
  • Hypomorphic mutations may impair but not abolish NEMO protein function. (medscape.com)
  • Mutations in the Neuraminidase-Like Protein of Bat Influenza H18N11 Virus Enhance Virus Replication in Mammalian Cells, Mice, and Ferrets. (cdc.gov)
Inhibitory3
  • A screen profiling the inhibitory activity of 178 commercially available kinase inhibitors at 0.5µM against a panel of 300 recombinant protein kinases using the Reaction Biology Corporation Kinase Hotspot SM platform. (guidetopharmacology.org)
  • maintains circulating platelets in a resting state by phosphorylating proteins in numerous platelet inhibitory pathways when in complex with NF-kappa-B (NFKB1 and NFKB2) and I-kappa-B-alpha (NFKBIA), but thrombin and collagen disrupt these complexes and free active PRKACA stimulates platelets and leads to platelet aggregation by phosphorylating VASP. (rcsb.org)
  • Active PKN2 phosphorylates PI3KC2-β to trigger PI3KC2-β complex formation with inhibitory 14-3-3 proteins. (nature.com)
Genes2
  • This protein was found to be a tissue-specific extinguisher that down-regulates the expression of seven liver genes in hepatoma x fibroblast hybrids. (nih.gov)
  • Genes that suppress carcinogenesis have been classified as gatekeepers that regulate cellular proliferation and cell death and as caretakers that are primarily encode DNA repair proteins required for the maintenance of genome integrity ( 15 ). (amegroups.org)
Oligomeric protein2
  • This manuscript reports the fundamental finding that an oligomeric protein kinase, CaMKII, can be phosphorylated by another molecule of the holoenzyme in a manner that does not involve subunit exchange. (elifesciences.org)
  • A multifunctional calcium-calmodulin-dependent protein kinase subtype that occurs as an oligomeric protein comprised of twelve subunits. (curehunter.com)
Inhibitors5
  • A series of flavonoid compounds was tested as inhibitors of protein kinase CK2. (nih.gov)
  • A screen of 72 inhibitors against 456 human kinases. (guidetopharmacology.org)
  • 2010) Activation state-dependent binding of small molecule kinase inhibitors: structural insights from biochemistry. (guidetopharmacology.org)
  • Selective inhibitors of protein kinase CK2 with significant cytotoxicity on tumor cells based on a 2-aminothiazole scaffold were described recently. (rcsb.org)
  • Medium-Throughput Detection of Hsp90/Cdc37 Protein-Protein Interaction Inhibitors Using a Split Renilla Luciferase-Based Assay. (nih.gov)
Monoclonal2
  • Mouse monoclonal to cAMP Protein Kinase Catalytic subunit - Store at -20°C or lower. (extinct-website.com)
  • Immunoprecitation demonstrated that a TcPKAc monoclonal antibody was able to pull-down proteins recognized by trans-sialidase antibodies as well as a SA85-1.1 antibody and vice versa. (elsevierpure.com)
Enzyme2
CaMKII6
  • The dodecameric protein kinase CaMKII is expressed throughout the body. (elifesciences.org)
  • The prevailing hypothesis for the spread of CaMKII activity, involving shuffling of subunits between activated and naive holoenzymes, is broadly termed subunit exchange. (elifesciences.org)
  • B ) Cartoon representation of activation of CaMKII by calcium:calmodulin (top) and proposed mechanism for spread of kinase activity (bottom). (elifesciences.org)
  • C ) Schematic representation of experiment performed in panel ( D ). ( D ) Kinase activity of CaMKII WT (10 nM) against CaMKII KD (4 μM). (elifesciences.org)
  • The Ca2+/calmodulin-dependent protein kinases (CaM kinases) comprise a structurally related subfamily of serine/threonine kinases which include CaMKI, CaMKII and CaMKIV. (thermofisher.com)
  • CaMKII is an ubiquitously expressed serine/threonine protein kinase that is activated by Ca2+ and calmodulin (CaM) and has been implicated in regulation of the cell cycle and transcription. (thermofisher.com)
MRNA4
  • Expression of DNA-PKcs mRNA was detected by semi-quantitative reverse transcription polymerase chain reaction (RT-PCR) and DNA-PKcs protein by Western blot in 62 CML patients and K562, as compared to those of 23 normal individual controls. (hku.hk)
  • In 26 CML patients received allogeneic peripheral blood stem cell transplantation (allo-PBSCT) and 4 CML patients treated with imatinib, the expression of bcr-abl mRNA and DNA-PKcs protein was detected by RT-PCR and Western blot, respectively. (hku.hk)
  • In 26 CML patients received allo-PBSCT and 4 CML patients treated with imatinib, the expression of DNA-PKcs protein was enhanced while the expression of bcr-abl mRNA decreased. (hku.hk)
  • Treatment with NU7026 did not alter the production of reactive oxygen species and oxidative stress by hydroquinone but repressed the protein level of DNA-PKcs and blocked the induction of the kinase mRNA and protein expression by hydroquinone. (cdc.gov)
Amino acids2
  • The chemical activity of a kinase involves removing a phosphate group from ATP and covalently attaching it to one of three amino acids that have a free hydroxyl group. (kinaseresearch.com)
  • There are also protein kinases that phosphorylate other amino acids, including that phosphorylate histidine residues. (kinaseresearch.com)
Intracellular1
Ubiquitous protein2
  • CK2 is a ubiquitous protein kinase involved in many cell functions. (nih.gov)
  • NEMO is an ubiquitous protein that becomes active during embryogenesis. (medscape.com)
Antibody1
  • In addition, a phospho-(Ser/Thr) PKA substrate antibody detected bands on immunoblot analysis of trans-sialidase antibody precipitated proteins from parasite lysate and the media of L 6 E 9 myoblasts infected with trypomastigotes as well as from a SA85-1.1 antibody precipitated proteins from parasite lysate. (elsevierpure.com)
Molecular1
Ataxia1
  • Activation of DNA-PKcs by UV is dependent on ATR (Ataxia telangiectasia mutated and Rad3 related) kinase and is important for replication stress [ 12 ]. (biomedcentral.com)
Multiprotein2
  • The IKB is phosphorylated by a multiprotein complex with two kinases subunits. (medscape.com)
  • Mechanistic target of rapamycin (mTOR) kinase functions in two multiprotein complexes: lysosomal mTOR complex 1 (mTORC1) and mTORC2 at the plasma membrane. (nature.com)
Human2
Autophosphorylation1
  • Its elaborate subunit organization and propensity for autophosphorylation allow it to preserve neuronal plasticity across space and time. (elifesciences.org)
Synaptic1
  • Neurons also contain other types of neurotransmitter receptors, such as those coupled via G-proteins to second-messenger cascades, that can modulate synaptic transmission and/or membrane excitability. (jneurosci.org)
Replication1
  • A nonconventional nuclear localization sequence (NLS) has been found for this protein which suggests a role in DNA replication via the protein serving as a nuclear transport protein for the second subunit of the Replication Factor C (RFC40). (nih.gov)
Role in the regulation1
  • Studies of the pig counterpart suggest that this subunit may play a key role in the regulation of energy metabolism in skeletal muscle. (ageing-map.org)
Neurons1
  • Is the appropriate distribution of G-protein-coupled receptors regulated by interactions with neurons that synthesize and release the neuromodulator? (jneurosci.org)
Differentiation2
  • Protein kinase A (PKA) has been suggested as a regulator of stage differentiation in Trypanosoma cruzi. (elsevierpure.com)
  • Prior studies of acute phosphate restriction during the endochondral phase of fracture healing showed delayed chondrocyte differentiation was mechanistically linked to decreased bone morphogenetic protein signaling. (researchgate.net)
Activity3
  • 2013) A broad activity screen in support of a chemogenomic map for kinase signalling research and drug discovery. (guidetopharmacology.org)
  • have reported that increased DNA-PKcs activity can suppress HR repair in G1 phase of cells through increasing RBX1 protein expression, which prompts the neddylation and activity of cullin 1, a key component of the Skp1-Cullin1-F-box ubiquitin 3 ligase, consequently mediating ubiquitination and degradation of EXO1. (molcells.org)
  • Raw data for results from screening kinase activity towards T279 of PI3KC2-β are provided in Supplementary Table 2 . (nature.com)
Regulate cell1
Nuclear1
  • Activation of the transcription factor nuclear factor KB (NF-KB) requires the NEMO protein. (medscape.com)
Activation4
  • The NEMO protein is required for the activation of the kinase complex. (medscape.com)
  • Fig. 2: PKN2 facilitates mTORC1 activation by phosphorylating PI3KC2-β and triggering sequestration by 14-3-3 proteins. (nature.com)
  • In contrast to the expectations of previous work, we found little evidence for subunit exchange upon activation, and no effect of restraining subunits to their parent holoenzymes. (elifesciences.org)
  • Our results place fundamental limits on the activation mechanism of this kinase. (elifesciences.org)
Synthesis1
  • We demonstrate that protein kinase N (PKN) facilitates mTORC1 signalling by repressing PI3KC2-β-mediated phosphatidylinositol-3,4-bisphosphate synthesis downstream of mTORC2. (nature.com)
Complex1
  • It has been shown to form complex with Hsp90 and a variety of protein kinases including CDK4, CDK6, SRC, RAF-1, MOK, as well as eIF2 alpha kinases. (nih.gov)