• Professor Forsburg studies how chromosome duplication and maintenance contributes to overall genome stability using a simple model genetic system, the fission yeast Schizosaccharomyces pombe . (usc.edu)
  • However, a Borealin homologue has never been characterized in the fission yeast, Schizosaccharomyces pombe. (uky.edu)
  • Thus, this study characterizes a fission yeast Borealin homologue and reveals a previously unrecognized connection between the CPC and the process of cytokinesis in S. pombe. (uky.edu)
  • To facilitate a more comprehensive analysis, we constructed a genome-scale epistasis map (E-MAP) for the fission yeast Schizosaccharomyces pombe, providing phenotypic signatures for ∼60% of the nonessential genome. (thebiogrid.org)
  • Stress-activated protein kinase pathway functions to support protein synthesis and translational adaptation in response to environmental stress in fission yeast. (ox.ac.uk)
  • Mitotic regulation of protein phosphatases by the fission yeast sds22 protein. (anticorps-enligne.fr)
  • Fission yeast condensin complex: essential roles of non-SMC subunits for condensation and Cdc2 phosphorylation of Cut3/SMC4. (anticorps-enligne.fr)
  • The budding yeast Saccharomyces cerevisiae and fission yeast Schizosaccharomyces pombe have Yta7 and Abo1 as orthologs of ATAD2, respectively. (molcells.org)
  • Mitochondrial ribosomal proteins were studied best in yeast, where the small subunit was shown to contain about 35 proteins. (pasteur.fr)
  • Using a recently described affinity purification technique and tagged versions of yeast Ykl155c and Mrp1, we isolated this mitochondrial ribosomal subunit and identified a total of 20 proteins, of which 12 are new. (pasteur.fr)
  • For a subset of the newly described ribosomal proteins, we showed that they are localized in mitochondria and are required for the respiratory competency of the yeast cells. (pasteur.fr)
  • The origin recognition complex (ORC) was originally identified in the yeast Saccharomyces cerevisiae as a protein that specifically binds to origins of DNA replication. (embl.de)
  • The C-terminal domain shows strong sequence similarity to human, frog, and yeast Orc4 proteins, including conserved ATP-binding motifs. (embl.de)
  • Homologues of the yeast Oxa1 protein are found in chloroplasts of plants and in a wide variety of bacteria. (tcdb.org)
  • AMP-activated protein kinase - 5 AMP activated protein kinase or AMPK consists of three proteins (subunits) that together make a functional enzyme, conserved from yeast to humans, that plays a role in cellular energy homeostasis. (en-academic.com)
  • Term] id: PR:P41811 name: coatomer subunit beta' (yeast) def: "A coatomer subunit beta' that is encoded in the genome of yeast. (proconsortium.org)
  • SEC27 (yeast) [Term] id: PR:000019417 name: coatomer subunit beta', initiator methionine removed form def: "A coatomer subunit beta' that has had the initiator methionine removed. (proconsortium.org)
  • Mitochondrial distribution and morphology protein 12 OS=Schizosaccharomyces pombe (strain 972 / ATCC. (yeastrc.org)
  • Mitochondrial distribution and morphology protein 12 OS=Candida glabrata (strain ATCC 2001 / CBS 138. (yeastrc.org)
  • Mitochondrial distribution and morphology protein 12 OS=Ashbya gossypii (strain ATCC 10895 / CBS 109. (yeastrc.org)
  • Mitochondrial distribution and morphology protein 12 OS=Kluyveromyces lactis (strain ATCC 8585 / CBS. (yeastrc.org)
  • Mitochondrial distribution and morphology protein 12 OS=Candida albicans (strain SC5314 / ATCC MYA-2. (yeastrc.org)
  • Protein kinases, the enzymes responsible for protein phosphorylation, make up almost 2% of protein-encoding genes in the human genome [ 1 ] and an estimated 30-50% of human proteins are phosphorylated [ 2 ]. (biomedcentral.com)
  • The genome sequence of Schizosaccharomyces pombe. (genscript.com)
  • The gene set of the last common ancestor of the crown group consists of 3,413 KOGs and largely includes proteins involved in genome replication and expression, and central metabolism. (biomedcentral.com)
  • Here, we examine the MICOS complex in Schizosaccharomyces pombe, a minimal model whose genome only encodes for four core subunits. (bvsalud.org)
  • The three genes encoding these proteins were inserted individually into vaccinia virus GLV-1h68 genome under the control of an identical promoter. (uni-wuerzburg.de)
  • Mitochondrial TAT transports folded proteins in those eukaryotes with TatA and TatC subunits encoded in the mitochondrial genome. (tcdb.org)
  • initiator methionine [Term] id: PR:Q9CAA0 name: coatomer subunit beta'-1 (Arabidopsis thaliana) def: "A coatomer subunit beta' that is encoded in the genome of Arabidopsis thaliana. (proconsortium.org)
  • At1g79990 (Arabidopsis thaliana) [Term] id: PR:Q54YD8 name: coatomer subunit beta' (Dictyostelium discoideum) def: "A coatomer subunit beta' that is encoded in the genome of Dictyostelium discoideum. (proconsortium.org)
  • copB2 (Dictyostelium discoideum) [Term] id: PR:O62621 name: coatomer subunit beta' (fruit fly) def: "A coatomer subunit beta' that is encoded in the genome of fruit fly. (proconsortium.org)
  • Centromere protein H is a protein that in humans is encoded by the CENPH gene. (wikipedia.org)
  • The protein encoded by this gene colocalizes with inner kinetochore plate proteins CENP-A and CENP-C in both interphase and metaphase. (wikipedia.org)
  • Glutaminase liver isoform, mitochondrial is a protein in humans that is encoded by GLS2 gene. (cusabio.com)
  • We isolated the Schizosaccharomyces pombe zfs1 gene as a multicopy suppressor of the sterility caused by overexpression of a double-stranded RNase. (elsevierpure.com)
  • The deduced zfs1 gene product of 404 amino acids showed similarity to a mouse growth factor-inducible nuclear protein Nup475. (elsevierpure.com)
  • When a cell needs to make a particular protein, it first copies the instructions from the matching gene into a molecule known as a messenger RNA (or an mRNA for short). (elifesciences.org)
  • Background: This gene encodes one of seven subunits of the human Arp2/3 protein complex. (icbinhibitor.com)
  • This subunit is a member of the SOP2 family of proteins and is most similar to the protein encoded by gene ARPC1B. (icbinhibitor.com)
  • Giuliano G, Pichersky E, Malik V, Timko M, Scolnik P, Cashmore A (1988) An evolutionarily conserved protein binding sequence upstream of a plant light-regulated gene. (edu.pl)
  • Hadwiger J, Wittenberg C, Mendenhall M, Reed S (1989) The saccharomyces cerevisiae cks1 gene, a homolog of the schizo-saccharomyces pombe suc1+ gene, encodes a subunit of the cdc28 protein kinase complex. (edu.pl)
  • Nearly all these proteins are subunits of known or predicted multiprotein complexes, in agreement with the balance hypothesis of evolution of gene copy number. (biomedcentral.com)
  • The stress-activated protein kinase (SAPK) pathway plays a central role in coordinating gene expression in response to diverse environmental stress stimuli. (ox.ac.uk)
  • The origin of the eukaryotic N- glycosylation pathway is not unique and less straightforward than previously thought: some basic components likely have proteoarchaeal origins, but the pathway was extensively developed before the eukaryotic diversification through multiple gene duplications, protein co-options, neofunctionalizations and even possible horizontal gene transfers from bacteria. (biomedcentral.com)
  • coatomer subunit beta', initiator methionine removed form [Term] id: PR:000005735 name: coatomer subunit beta' def: "A protein that is a translation product of the human COPB2 gene or a 1:1 ortholog thereof. (proconsortium.org)
  • Predikin now consists of two components: (i) PredikinDB, a database of phosphorylation sites that links substrates to kinase sequences and (ii) a Perl module, which provides methods to classify protein kinases, reliably identify substrate-determining residues, generate scoring matrices and score putative phosphorylation sites in query sequences. (biomedcentral.com)
  • New features in Predikin include the use of SQL queries to PredikinDB to generate predictions, scoring of predictions, more reliable identification of substrate-determining residues and putative phosphorylation sites, extended options to handle protein kinase and substrate data and an improved web interface. (biomedcentral.com)
  • The post-translational modification of proteins by phosphorylation of serine, threonine or tyrosine residues is a ubiquitous process in cellular regulation. (biomedcentral.com)
  • Although recruitment of the capping enzymes to the transcription complex is dependent on phosphorylation of the C-terminal domain of the Rpb1 subunit of polymerase II (Po1-II), there may be additional levels of control that coordinate capping with elongation. (mskcc.org)
  • Reduced translation initiation correlated with phosphorylation of the alpha subunit of eukaryotic initiation factor 2 (eIF2alpha) in wild-type cells. (ox.ac.uk)
  • Phosphorylation of dis2 protein phosphatase at the C-terminal cdc2 consensus and its potential role in cell cycle regulation. (anticorps-enligne.fr)
  • Phosphorylation of Eukaryotic Initiation Factor 2 by Heme-Regulated Inhibitor Kinase-Related Protein Kinases in Schizosaccharomyces pombe Is Important for Resistance to Environmental Stresses. (en-academic.com)
  • We have classified these motifs into three types according to their sequence similarity and have found that they are prevalent in many eukaryotic nuclear proteins in single or multiple copies. (embl.de)
  • More than half of all eukaryotic proteins are glycoproteins, and 90 % of those are N- glycosylated [ 33 ]. (biomedcentral.com)
  • Caenorhabditis elegans , Drosophila melanogaster , Homo sapiens , Arabidopsis thaliana , Saccharomyces cerevisiae , Schizosaccharomyces pombe and Encephalitozoon cuniculi . (biomedcentral.com)
  • Using mass spectrometric analyses of Saccharomyces cerevisiae ribosomes, we found that the amino acid residue in closest proximity to the decoding center, Pro-64 of the 40S subunit ribosomal protein Rps23p (RPS23 Pro-62 in humans) undergoes posttranslational hydroxylation. (ox.ac.uk)
  • The RPS23 hydroxylases in S. cerevisiae (Tpa1p), Schizosaccharomyces pombe and green algae catalyze an unprecedented dihydroxylation modification. (ox.ac.uk)
  • We have previously described an approach to predicting the substrate specificity of serine-threonine protein kinases. (biomedcentral.com)
  • The new features significantly enhance the ability of Predikin to analyse protein kinases and their substrates. (biomedcentral.com)
  • Protein kinases and their substrates regulate essentially all cellular processes through complex regulatory networks, in which phosphorylated proteins act as switches that tune the response of the cell to environmental stimuli. (biomedcentral.com)
  • Defects in these networks result in a variety of disease states making protein kinases important targets for drug design [ 3 ]. (biomedcentral.com)
  • Crystal structures of protein kinases with bound substrate peptides show that substrate residues at positions -3 to +3 relative to the phosphorylated serine, threonine or tyrosine residue adopt an extended conformation and bind to a pocket in the catalytic domain of the protein kinase [ 8 ]. (biomedcentral.com)
  • The relative contribution of substrate recruitment and peptide specificity to protein kinase substrate specificity varies between protein kinases. (biomedcentral.com)
  • The prediction of peptide specificity is therefore the basis for most of the available computational methods aimed at predicting substrates of protein kinases. (biomedcentral.com)
  • Intracellular signaling protein kinases that play a signaling role in the regulation of cellular energy metabolism. (reference.md)
  • AMP-activated protein kinases modify enzymes involved in LIPID METABOLISM, which in turn provide substrates needed to convert AMP into ATP. (reference.md)
  • Egan EA, Solomon MJ (1998) Cyclin-stimulated binding of CKS proteins to cyclin-dependent kinases. (edu.pl)
  • Mitochondrial distribution and morphology protein 12 OS=Yarrowia lipolytica (strain CLIB 122 / E 150. (yeastrc.org)
  • Homologues could be found, however, in predicted protein sequences from Schizosaccharomyces pombe. (pasteur.fr)
  • Known as "destabilising motifs", these sequences attract the attention of a group of proteins called Ccr4-Not. (elifesciences.org)
  • These sequences represent the protein coding region of the yaf9 cDNA ORF which is encoded by the open reading frame (ORF) sequence. (genscript.com)
  • Mitochondria import nuclearly-encoded proteins, made in the cell cytoplasm, into the mitochondrial matrix where their mitochondrial targeting sequences are removed by proteolysis. (tcdb.org)
  • Bacteria also export (to the periplasm) N-tails of membrane proteins synthesized without leader sequences by a Sec (Type IIPS)-independent mechanism. (tcdb.org)
  • A previous population genomic analysis showed that protein sequences are under greater functional constraints in thermophiles than in mesophiles, suggesting that mutations are functionally more deleterious in thermophiles than in mesophiles [ 4 ]. (nature.com)
  • CENP-H shows sequence similarity to the Schizosaccharomyces pombe kinetochore protein Fta3 which is a subunit of the Sim4 complex. (wikipedia.org)
  • Nbl1 (novel Borealin-like 1) physically associates with known CPC components, affects the kinase activity and stability of the S. pombe Aurora B homologue, Ark1, colocalizes with known CPC subunits during mitosis, and shows sequence similarity to human Borealin. (uky.edu)
  • 2007 ). Several Oxa1-type insertases in the ER serve as catalytically active core subunits in the ER membrane protein complex (EMC), the guided entry of tail-anchored (GET) and the GET- and EMC-like (GEL) complex. (tcdb.org)
  • Here, we isolate a previously uncharacterized S. pombe protein through association with the Cdc14 phosphatase homologue, Clp1/Flp1, and identify it as a Borealin-like member of the CPC. (uky.edu)
  • and the contribution of chromatin-modifying proteins to replication and repair. (usc.edu)
  • Many viruses use the microtubule transport system to transport nucleic acid/protein cores to intracellular replication sites after invasion host the cell membrane. (wikipedia.org)
  • pombe replication origin, ars1. (embl.de)
  • pombe origins of replication, which differ significantly from those of Sa. (embl.de)
  • These data identify a protein homeostasis pathway critical for Toxoplasma replication and fitness and suggest an unappreciated role for the parasite residual body in compartmentalizing processes that threaten the fidelity of parasite development. (bvsalud.org)
  • Replication protein A 70 kDa DNA-binding subunit C OS=Oryza sativa subsp. (uu.nl)
  • We find that Spt5 is essential for viability of S. pombe and that it interacts in vivo with S. pombe Spt4 via a central domain distinct from the Spt5 CTD. (mskcc.org)
  • The second factor, termed peptide specificity, describes the interaction between amino acid residues in the catalytic domain of the protein kinase and the substrate residues that surround the phosphorylated residue. (biomedcentral.com)
  • However, it is recognised that for many protein kinase families, particularly those that phosphorylate Ser/Thr residues, peptide specificity is the major factor that determines substrate specificity. (biomedcentral.com)
  • RNA-binding proteins contribute to specificity by interacting with both Ccr4-Not and target mRNAs, but this is not fully understood. (elifesciences.org)
  • This brings to 26 the total number of proteins described as components of the mitochondrial small ribosomal subunit. (pasteur.fr)
  • Remarkably, almost half of the previously and newly identified mitochondrial ribosomal components showed no similarity to any known ribosomal protein. (pasteur.fr)
  • Another newly identified ribosomal protein, Ygl129c, was previously shown to be a member of the DAP-3 family of mitochondrial apoptosis mediators. (pasteur.fr)
  • Using an unbiased proteomics approach, we identify a poorly characterized inner mitochondrial membrane protein that interacts with MICOS and is required to maintain cristae morphology, which we name Mmc1. (bvsalud.org)
  • The matrix exposed C-terminal α-helical domain of Oxa1 can bind mitochondrial ribosomes to facilitate co-translational insertion of proteins into the mitochondrial membrane ( Jia et al . (tcdb.org)
  • In more distant species, putative homologues were detected for Ykl155c, which shares conserved motifs with uncharacterized proteins of higher eukaryotes including humans. (pasteur.fr)
  • Its C-terminal region carried two putative zinc-fingers, both of which should be intact for the protein to be functional as the suppressor. (elsevierpure.com)
  • First identified in Drosophila, the conserved Polycomb group (Pc-G) protein complexes were shown to be both writers (Polycomb Repressive Complex 2, PRC2) and readers (Polycomb Repressive Complex 1, PRC1) of H3K27me3 [ 19 , 20 , 21 ]. (biomedcentral.com)
  • 2001 ). Mitochondria have two, one for co-translational, and one for post-translational insertion of membrane proteins. (tcdb.org)
  • In these reactions, precursor proteins converted themselves into active enzymes that were not peptidases. (ebi.ac.uk)
  • Processing of the precursor proteins exposes an N-terminal threonine residue that is the nucleophile in catalysis ( Aronson, 2004 ). (ebi.ac.uk)
  • It is most interesting that this motif seems to be quite specific to known or predicted chromosomal/DNA-binding proteins, suggesting that it may act as a versatile minor groove tether. (embl.de)
  • The more mRNA copies it makes, the more protein it can produce. (elifesciences.org)
  • A simple way to control protein production is to raise or lower the number of these mRNA messages, and living cells have lots of ways to make this happen. (elifesciences.org)
  • Schizosaccharomyces pombe YEATS family histone acetyltransferase subunit Yaf9 (yaf9), mRNA. (genscript.com)
  • In this fundamental process of life, the ribosome builds proteins according to the information encoded on the mRNA. (en-academic.com)
  • The C-terminal domain of the 990-amino acid Schizosaccharomyces pombe Spt5 protein, composed of repeats of a nonapeptide motif (consensus sequence TPAWNSGSK), is necessary and sufficient for binding to the capping enzymes in vivo (in a two-hybrid assay) and in vitro. (mskcc.org)
  • Small DNA-binding motif first described in the high mobility group non-histone chromosomal protein HMG-I(Y). (embl.de)
  • The AT-hook is a small DNA-binding protein motif which was first described in the high mobility group non-histone chromosomal protein HMG-I(Y). Since its discovery, this motif has been observed in other DNA-binding proteins from a wide range of organisms. (embl.de)
  • Furthermore, AT-hook motifs are frequently associated with known functional domains seen in chromatin proteins and in DNA-binding proteins (e.g. histone folds, homeodomains and zinc fingers). (embl.de)
  • The Orp4 protein (and its isolated N-terminal domain) binds to the Sc. (embl.de)
  • Det innebär att en eller flera fettsyror binds till proteinets N-terminal vilket senare utgör en signal till transportsystemet (LolACDE) att proteinet ska transporteras över periplasman. (umu.se)
  • Together, these four subunits interdependently regulate CPC function, and they are highly conserved among eukaryotes. (uky.edu)
  • The N- glycosylation is an essential protein modification taking place in the membranes of the endoplasmic reticulum (ER) in eukaryotes and the plasma membranes in archaea. (biomedcentral.com)
  • While a postdoctoral student at MIT, Tomomi Kiyomitsu discovered how dynein has a role as a motor protein in aligning the chromosomes in the middle of the cell during the metaphase of mitosis. (wikipedia.org)
  • Centromere and kinetochore proteins play a critical role in centromere structure, kinetochore formation, and sister chromatid separation. (wikipedia.org)
  • We analyzed the incorporation of sister chromatid cohesion proteins (cohesins) and other AE components into AEs. (rupress.org)
  • Yet, genetic and biochemical studies identified only 14 proteins, half of which were predictable by sequence homology with prokaryotic ribosomal components of the small subunit. (pasteur.fr)
  • In the past decades, great efforts were made to understand the metabolic strategies deep-sea hyperthermophiles use to conserve energy and cope with physicochemical stresses, and to appreciate the molecular mechanisms leading to the stabilization of nucleic acids and proteins at exceedingly high temperatures [ 2 , 3 ]. (nature.com)
  • Listed below are up to the top 10 sequence alignment matches, by species, for the PSI-BLAST search against the protein sequence for MDM12 . (yeastrc.org)
  • The approximately 40% of KOGs that are represented in six or seven species are enriched in proteins responsible for housekeeping functions, particularly translation and RNA processing. (biomedcentral.com)
  • 2022 ). The YidC transmembrane (TM) groove is essential for a high- affinity interaction, and the hydrophilic nature of the YidC groove plays an important role in protein transport across the cytoplasmic membrane bilayer to the periplasmic side. (tcdb.org)
  • It is thought that this protein can bind to itself, as well as to CENP-A, CENP-B or CENP-C. Multimers of the protein localize constitutively to the inner kinetochore plate and play an important role in the organization and function of the active centromere-kinetochore complex. (wikipedia.org)
  • Two major factors determine the formation of a protein kinase-substrate complex [ 4 ]. (biomedcentral.com)
  • The similarity between these two proteins suggests that they both may function as p41 subunit of the human Arp2/3 complex that has been implicated in the control of actin polymerization in cells. (icbinhibitor.com)
  • It is possible that the p41 subunit is involved in assembling and maintaining the structure of the Arp2/3 complex. (icbinhibitor.com)
  • Multiple versions of the p41 subunit may adapt the functions of the complex to different cell types or developmental stages. (icbinhibitor.com)
  • While MICOS is required for normal cristae morphology, the precise mechanistic role of each of the seven human MICOS subunits, and how the complex coordinates with other cristae shaping factors, has not been fully determined. (bvsalud.org)
  • 2018 ). Through YidC, proteins are inserted into the lipid bilayer via the SecYEG-dependent complex, but YidC functions as a chaperone in protein folding processes. (tcdb.org)
  • clathrin assembly protein complex 1, me. (cornell.edu)
  • AT-hook motifs identified in a wide variety of DNA-binding proteins. (embl.de)
  • Using pattern searches and position-dependent matrices, we have extracted the AT-hook motifs present in a non-redundant protein sequence database. (embl.de)
  • This highlights the possibility that functionally analogous proteins work with MICOS to establish cristae morphology in metazoans. (bvsalud.org)
  • Our study reveals a new class of proteins that evolved early in fungal phylogeny and is required for the maintenance of cristae architecture. (bvsalud.org)
  • We examined the role of this pathway in the translational response to stress in Schizosaccharomyces pombe. (ox.ac.uk)
  • Protein synthesis was further reduced in mutants disrupting the SAPK pathway, including the mitogen-activated protein kinase Wis1 or the mitogen-activated protein kinase Spc1/Sty1, suggesting a role for these stress response factors in this translational control. (ox.ac.uk)
  • The chloroplast albino 3 (ALB3) protein appears to integrate the light harvesting chlorophyll-binding protein into thylakoid membranes using a pathway that is distinct from the chloroplast Sec translocation pathway. (tcdb.org)
  • Transgenic retina-specific expression of AD5 reveals that stable AD5 protein is initially localized in the nucleus but later found in the cytoplasm concurrent with progressing rod outer segment degeneration and apoptosis. (uni-wuerzburg.de)
  • Insertion of a membrane protein via the Oxa1p export machinery follows the 'positive-inside' rule for membrane protein topology. (tcdb.org)
  • It is involved in the assembly of kinetochore proteins, mitotic progression and chromosome segregation. (wikipedia.org)
  • RNAi knockdown of human kinetochore protein CENP-H". Biochemical and Biophysical Research Communications. (wikipedia.org)
  • The comparison of several polyisoprenol-based glycosylation pathways from the three domains of life shows that most of the implicated proteins belong to a limited number of superfamilies. (biomedcentral.com)