• An international team led by investigators in Estonia did pyrosequencing on fungal DNA from more than 1,000 plant root samples, looking at the symbiotic fungi from plants at diverse environmental sites around the world. (genomeweb.com)
  • From the DNA-based fungal taxa they identified, the researchers found that communities of these so-called "arbuscular mycorrhizal" fungi tend to coincide with local environmental conditions and along spatial gradients. (genomeweb.com)
  • Likewise, when researchers folded in data from an existing arbuscular mycorrhizal fungal virtual taxa database, they found that far more fungi than plant species are shared between continents, though arbuscular mycorrhizal fungal community diversity did seem to diminish with increasing distance from the equator. (genomeweb.com)
  • Their phylogenetic analysis, based on small subunit ribosomal RNA gene sequences, suggested most current fungal symbionts come from taxa that appeared after continents moved apart, fueling speculation about how slow-moving organisms from the taxa may have reached such diverse and widespread locations. (genomeweb.com)
  • We suggest that addressing both global and local dispersal of [arbuscular mycorrhizal] fungi and the role of dispersal agents such as birds, large mammals (including humans), wind, surface water, and seawater can illuminate the processes governing [arbuscular mycorrhizal] fungal diversity patterns," the study's authors concluded. (genomeweb.com)
  • Recently, DNA sequencing of orthologous genes has greatly improved our knowledge of fungal phylogeny. (pensoft.net)
  • Together, these rate differences at the gene and species levels may increase long-branch-attraction (LBA between the two fast-evolving groups) thus causing weaker support for fungal monophyly and the nucleariid-fungal sister relationship, or predicting altogether incorrect phylogenetic relationships. (biomedcentral.com)
  • construction of a shuttle vector for heterologous expression of a novel fungal α-amylase gene in aspergillus oryzae. (liverpool.ac.uk)
  • A positionally conserved ORF has been found on the complementary strand of the cox1 genes of both eukaryotic mitochondria (protist, plant, fungal and animal) and alpha-proteobacteria. (biomedcentral.com)
  • New gene-based technologies can now be employed to examine microbial diversity through the use of small sub-unit ribosomal DNA analysis (e.g. 16S rDNA) and to understand the function of complex microbial ecosystems in the rumen through metagenomic analysis. (nhbs.com)
  • 2007. Intra-specific and intra-sporocarp ITS variation of ectomycorrhizal fungi as assessed by rDNA sequencing of sporocarps and pooled ectomycorrhizal roots from a Quercus woodland. (dannyhaelewaters.com)
  • Data from ITS rDNA and the genes coding for translation elongation factor 1-α and DNA replication licensing factor mini-chromosome maintenance complex 7 support the distinctness of Mycoblastus s. str. (cambridge.org)
  • rRNA genes contain regions of variable DNA sequence that are unique to the species carrying the … Ribosomal RNA is transcribed from ribosomal DNA (rDNA) and then bound to ribosomal proteins to form small and large ribosome subunits. (printerresource.com)
  • In the summer of 2014 and winter of 2015, we collected that the same species of fungus occurs on all 3 continents. (cdc.gov)
  • Based on more than 912,000 quality-filtered reads generated by Roche 454 sequencing for 836 samples, the researchers defined almost 250 DNA-based "virtual taxa" associated with 161 plant species - a symbiotic fungi set that included 68 percent of the previously described arbuscular mycorrhizal taxa and 10 new taxa. (genomeweb.com)
  • Suspected Basidiobolus species were tentatively identified using routine bench tests and phenotypes were authenticated by phylogenetic analysis of the large subunit ribosomal RNA gene. (academicjournals.org)
  • Molecular data from two species of the genus are analyzed using single and combined ITS and LSU gene datasets and the workflow of phylogenetic analysis is provided in an appendix. (asianjournalofmycology.org)
  • While much attention has been given to the prokaryotic species and their functional capacity 2 - 4 , microbial eukaryotes such as fungi also perform key roles in many environments, for example by making carbon available and by creating new environments 5 . (researchsquare.com)
  • Since the publication of Nova Plantarum Genera by Pier Antonio Micheli in 1737, marking the start of modern mycology, researchers have described approximately 148,000 species of fungi. (dannyhaelewaters.com)
  • Intraspecific ITS variability in the Kingdom Fungi as expressed in the international sequence databases and its implications for molecular species identification. (dannyhaelewaters.com)
  • Sometimes these different morphs of a single fungus were formerly thought to be separate species. (eol.org)
  • All of the sequenced genomes have a group I intron in the large ribosomal subunit RNA gene, suggesting that this intron is fixed in these species. (umd.edu)
  • Despite the conservation of the core genes, the mitochondrial genomes of Aspergillus and Penicillium species examined here exhibit significant amount of interspecies variation. (umd.edu)
  • In this context, it seems noteworthy that Nuclearia and fungi other than chytrids are fast-evolving, and that the rate of tubulin evolution varies strongly among species of the latter dataset (correlating to some degree with the independent loss of the flagellar apparatus in non-chytrid fungi and in Nuclearia ). (biomedcentral.com)
  • Each assay is based on an endpoint PCR amplification of a species-specific genetic region of the relevant microbe or a region of an individual microbial gene. (qiagen.com)
  • As is usually the case for most mitosporic fungi, morphological characters are inadequate for delimiting species within a genus and this creates a continuing demand of screening for additional taxonomic characters. (biomedcentral.com)
  • Genome annotation resulted in 10,703 putative genes. (usda.gov)
  • The sources of genome expansion include group I introns and accessory genes encoding putative homing endonucleases, DNA and RNA polymerases (presumed to be of plasmid origin) and hypothetical proteins. (umd.edu)
  • The number of putative Regulators (genes that regulate it) and Targets (genes it regulates) for the given locus, based on experimental evidence. (yeastgenome.org)
  • This putative gene has been named gau for g ene a ntisense u biquitous in mtDNAs. (biomedcentral.com)
  • In view to find putative mitochondrial genes in alternative reading frames on both coding and "presupposed" non-coding strands, numerous complete mtDNA genomes have been analyzed. (biomedcentral.com)
  • In this study, 242 bambusicolous Apiospora were isolated from various bamboo materials (branches, culms, leaves, roots, and shoots) and examined using DNA sequence similarity based on the internal transcribed spacer, 28S large subunit ribosomal RNA gene, translation elongation factor 1-alpha, and beta-tubulin regions. (korea.ac.kr)
  • The identity was supported by combined analyses of the internal transcribed spacer (ITS1, 5.8s and ITS2), large subunit (LSU) and small subunit (SSU) of the ribosomal RNA gene and protein loci of elongation factor-1 alpha (tef1-α), RNA polymerase II largest subunits (RPB1 and RPB2). (asianjournalofmycology.org)
  • NCBI-GenBank lists only five gene fragments, a total of 3534 bp as follows: 1122 bp of DNA dependent RNA Polymerase II second largest subunit, 1069 bp of rRNA subunits/ITS regions, 451 bp of calmodulin, 354 bp of histone-H3, 321 bp of elongation factor 1-alpha and 217 bp of the actin gene. (biomedcentral.com)
  • The most widely understood role of RNA is in protein synthesis, which includes messenger RNA (mRNA), transfer RNA (tRNA) and ribosomal RNA (rRNA) (see Chapter 2). (printerresource.com)
  • It is the process in which the protein is synthesized from the information contained in a molecule of messenger rna mrna. (web.app)
  • Using an RNA genome, many viruses are known to encode their genetic information. (printerresource.com)
  • High-throughput sequence analysis of total RNA prepared from cultures with large dsRNAs identified two novel RNA viruses with genome sequences of approximately 9.3 kbp, which were named Fusarium virguliforme dsRNA mycovirus 1 and Fusarium virguliforme dsRNA mycovirus 2. (illinois.edu)
  • The presence of bacteria, viruses, and fungi in water. (lookformedical.com)
  • Virus Helper 0 domande Viruses which enable defective viruses to replicate or to form a protein coat by complementing the missing gene function of the defective (satellite) virus. (lookformedical.com)
  • They include both DNA and RNA viruses as well viruses from humans and animals. (lookformedical.com)
  • Virus A Rna 0 domande Viruses whose genetic material is RNA. (lookformedical.com)
  • In a more recent phylogenetic investigation with four nuclear gene sequences (EF-1α, HSP70, actin and β-tubulin), nucleariids associate confidently with Fungi, but only when selecting two slow-evolving chytridiomycetes [ 18 ]. (biomedcentral.com)
  • A. Ribosomal RNA analysis StructRNAfinder - predicts and annotates RNA families in transcript or genome sequences. (printerresource.com)
  • Dimerized STATs then translocate to the nucleus, where they bind DNA in the promoter sequences of target genes to activate transcription. (medscape.com)
  • Phylogenetic dendrograms for 5442 bp and 4401 bp of RNA Polymerase II largest and second largest subunits, and for 5474 bp of the ribosomal RNA cistron of C. personatum are presented in relation to closely related fungi. (usda.gov)
  • In this study, culture-based methods and small subunit ribosomal RNA gene clone library approach were used to characterize microbial communities in MRF bulk fluid and associated biofilm samples collected from fluid systems in an automobile engine plant. (cdc.gov)
  • The microbial community was analyzed by small subunit 16S ribosomal RNA genes using microarray (PhyloChip) and by high-throughput amplicon pyrosequencing based on universal primers targeting bacterial, archaeal, and eukaryotic communities. (umass.edu)
  • The most commonly used molecular approach for exploring microbial diversity is based on the PCR amplification and sequencing of genes encoding small subunit ribosomal RNA (16S rRNA) directly from environmental sources [ 16 ]. (biomedcentral.com)
  • Here, we review the literature on culture-independent analysis of microbial diversity in the BE and explore what it tells us about the dynamics and distribution of bacteria and fungi in these environments. (biomedcentral.com)
  • This type of growth is known for clusters of small patches of fungus on irregularly shaped nodules full of single-celled yeast-like cells. (wikipedia.org)
  • These are the small subunit (SSU) and large subunit (LSU) ribosomal RNA gene regions. (dannyhaelewaters.com)
  • Multicopy gene-based assays targeting the ribosomal DNA intergenic spacer (IGS) or the mitochondrial small subunit (mtSSU) showed relatively high sensitivity (limit of detection [LOD] = 0.05 to 5 pg) compared with a single-copy gene (FvTox1)-based assay (LOD = 5 to 50 pg). (illinois.edu)
  • Typical triploblastic animal mtDNAs contain genes that encode the large and small subunit ribosomal RNAs, 22 transfer RNAs (tRNAs), and 13 proteins that are all components of the oxidative phosphorylation process: ATPase subunits 6 and 8 (ATP6 and 8), cytochrome b (Cyt b), cytochrome c oxidase subunits 1-3 (Cox1-3) and NADH dehydrogenase subunits 1-6 and 4L (ND1-6 and 4L). (biomedcentral.com)
  • In the 28S ribosomal RNA gene phylogenetic tree, the strains assembled in the subclade encompass Basidiobolus spp. (academicjournals.org)
  • A dominant negative mutant of the E. coli RNA helicase DbpA blocks assembly of the 50S ribosomal subunit. (colorado.edu)
  • 5' RNA helicase. (colorado.edu)
  • Most of this variation can be attributed to accessory genes and mobile introns, presumably acquired by horizontal gene transfer of mitochondrial plasmids and intron homing. (umd.edu)
  • The genus Basidiobolus contains large groups of terrestrial fungi including the etiological agents of human gastrointestinal basidiobolomycosis (GIB). (academicjournals.org)
  • The molecular identification was conducted using multi-gene sequence analysis of large subunit (LSU), the nuclear ribosomal internal transcribed spacer (ITS), beta-tubulin (beta-tubulin), and RNA polymerase II (rpb2) genes. (kib.ac.cn)
  • This method uses large alignments of tens to hundreds of genes to reconstruct evolutionary histories. (web.app)
  • The accompanying comparative analysis of these and related publicly available mitochondrial genomes reveals wide variation in size (25-36 Kb) among these closely related fungi. (umd.edu)
  • The two smallest sequenced genomes (A. terreus and P. chrysogenum) do not contain introns in protein-coding genes, whereas the largest genome (T. stipitatus), contains a total of eleven introns. (umd.edu)
  • A notable example is the replacement of an original multi-subunit bacteria-like RNA polymerase (inherited from the proto-mitochondrial ancestor and still encoded in certain jakobid - but no other - mitochondrial genomes) by a single-subunit bacteriophage T3/T7-like RNA polymerase, which directs mitochondrial transcription in virtually all eukaryotes [ 24 ]. (biomedcentral.com)
  • Virus 1 quesito Minute infectious agents whose genomes are composed of DNA or RNA, but not both. (lookformedical.com)
  • Apart from a typical gene content and organization, the Beauveria mt genomes contained several introns and had longer intergenic regions when compared with their close relatives. (biomedcentral.com)
  • This study indicates that mt genomes and in particular intergenic regions provide molecular phylogeny tools that combined with criteria of geographic and climatic origin can subdivide the B. bassiana s.l. entomopathogenic fungi into seven clusters with common climate characteristics. (biomedcentral.com)
  • These unresolved questions served as motivation for the current phylogenetic analyses that are based on broad taxon sampling, substantially more nuclear genes (available through expressed sequence tag (EST) or complete genome projects), and comparative analyses of nuclear and mitochondrial gene datasets. (biomedcentral.com)
  • Roughly 80 percent of land plants have some form of arbuscular mycorrhizal fungus nestled in their roots, helping them obtain nutrients, withstand limited water conditions, and/or ward off pathogens in exchange for carbon the plant produces, the team noted. (genomeweb.com)
  • Consequently, the fungi are believed to impact not only individual plants, but also between-plant relationships and plant community structures. (genomeweb.com)
  • Together, the information will allow research on the fungus and its interaction with peanut plants. (usda.gov)
  • Yap1 had an earlier expression in both smut-susceptible and -resistant plants, with no apparent correlation with the expression of antioxidant genes sod, cat, gpx, or external redox imbalance. (bvsalud.org)
  • In this study, six qPCR assays were compared in five independent laboratories using the same set of DNA samples from fungi, plants, and soil. (illinois.edu)
  • We found that the tricarboxylic acid cycle-associated enzyme α-ketoglutarate (α-KG) dehydrogenase (KGDH) entered the nucleus, where it interacted with various JMJs to regulate α-KG-dependent histone demethylations by JMJs, and thus controlled genome-wide gene expression in plants. (bvsalud.org)
  • in fungi and plants, vacuoles function as a storage and sequestration vessel for a wide variety of ions and are responsible for cytosolic ion homeostasis and responses to ionic shock. (liverpool.ac.uk)
  • functionally characterized nat transporters from bacteria, fungi, plants and mammals are ion-coupled symporters specific for the uptake of purines, pyrimidines and related analogues. (liverpool.ac.uk)
  • A., 1992, The use of 16S ribosomal RNA targeted oligonucleotide probes to study competition between ruminai fibrolytic bacteria. (printerresource.com)
  • Complementary oligonucleotide binding to the anticodon loop of fMet-transfer RNA. (colorado.edu)
  • Complementary oligonucleotide binding to transfer RNA. (colorado.edu)
  • The involvement of RNA in such fundamental processes as protein synthesis and RNA processing has led to the idea that ribozymes were more common in early life. (printerresource.com)
  • in the filamentous fungus aspergillus nidulans, however, little is known about the molecular genetic mechanisms of vacuolar biogenesis and function. (liverpool.ac.uk)
  • this is the first reported myosin ii mutation in a filamentous fungus. (liverpool.ac.uk)
  • the filamentous fungus aspergillus oryzae is a well-known expression host used to express homologous and heterologous proteins in a number of industrial applications. (liverpool.ac.uk)
  • Indeed, initial phylogenetic analyses based on single genes have been inconsistent in placing them even within opisthokonts. (biomedcentral.com)
  • pasop carries a selectable marker, pyrg, derived from aspergillus nidulans, and a strong promoter and a terminator of the amyb gene derived from a. oryzae. (liverpool.ac.uk)
  • This observation was confirmed with PCR amplification of 16S rRNA gene fragment using Mycobacteria specific primers. (cdc.gov)
  • Phylogenetic affiliations of mesopelagic acantharia and acantharian-like environmental 18S rRNA genes off the southern California coast. (mbl.edu)
  • Distel, D. L., DeLong, E. F., and Waterbury, J. Epub … Bacterial Identification by 16S rRNA Gene Sequence Analysis Background All bacteria contain 16S ribosomal RNA (rRNA) genes of approximately 1500 base pairs (bp) in length. (printerresource.com)
  • Pseudogymnoascus destructans , the causative agent tential P. destructans isolates on the basis of morphologic of WNS, is a cold-growing fungus that infects bats' skin characteristics. (cdc.gov)
  • Virulence and double-stranded RNA (dsRNA) profiles of 44 isolates of Fusarium virguliforme were compared. (illinois.edu)
  • The genome is expected to encode 8-10,000 genes, including all those required for a free-living eukaryote capable of multicellular development. (bioone.org)
  • Despite a disparity in gene content, all mtDNAs encode at least two components of the mitochondrial electron transport chain, including cytochrome c oxidase I (Cox1). (biomedcentral.com)
  • In some mtDNAs (e.g., nematodes and annelids), all genes are transcribed in the same direction, whereas, in others, both strands encode genes. (biomedcentral.com)
  • The mitochondrial gene content is highly variable across eukaryotes. (biomedcentral.com)
  • The leptospiral genome contains a broad array of genes encoding regulatory system, signal transduction and methyl-accepting chemotaxis proteins, reflecting the organism's ability to respond to diverse environmental stimuli. (bvsalud.org)
  • Genes related to toxins, lipoproteins and several surface-exposed proteins may facilitate a better understanding of the Leptospira pathogenesis and may serve as potential candidates for vaccine. (bvsalud.org)
  • Differential RNA-dependent ATPase activities of four rRNA processing yeast DEAD-box proteins. (colorado.edu)
  • In particular, it will help to establish which and how many developmental genes are either shared or specific to these two major eukaryotic groups. (biomedcentral.com)
  • The numerous modified nucleotides in eukaryotic ribosomal RNA. (printerresource.com)
  • This search has led to the observation of an unknown, positionally conserved open reading frame (ORF) on the complementary strand of eukaryotic cox1 genes. (biomedcentral.com)
  • The rimosus group is known for their cultivation of nodules of fungus, also called yeast gardens. (wikipedia.org)
  • In vitro, the fungus did not alter the expression of the transcriptional regulator Yap1 and the effector Pep1. (bvsalud.org)
  • Nine nuclear and four cytoplasmic genes were appropriate to design thirteen specific primers based on their nucleotide polymorphism. (ijpp.ir)
  • Identification of novel double-stranded RNA mycoviruses of Fusarium virguliforme and evidence of their effects on virulence. (illinois.edu)
  • the velvet complex in the gray mold fungus botrytis cinerea: impact of bclae1 on differentiation, secondary metabolism, and virulence. (liverpool.ac.uk)
  • Genomic sequence analysis reveals the presence of a competent transport system with 13 families of genes encoding for major transporters including a three-member component efflux system compatible with the long-term survival of this organism. (bvsalud.org)
  • Even so, not all that much is known about the nature and diversity of fungi found in association with plant roots, the researchers explained, beyond morphological descriptions of a few hundred arbuscular mycorrhizal fungi and DNA-based descriptions of hundreds more. (genomeweb.com)
  • Nucleariids lack distinctive morphological features that might allow associating them with either animals or fungi. (biomedcentral.com)
  • In this context, it is essential to determine the precise phylogenetic position of candidate protists that are close to Fungi, Metazoa, or opisthokonts as a whole. (biomedcentral.com)
  • Such knowledge is important not only for understanding the diversity and evolution of these fungi but also for identifying the most appropriate isolate(s) for commercial biological-control applications [ 3 ] [ 6 ] . (encyclopedia.pub)
  • NEW YORK (GenomeWeb) - Fungi involved in symbiotic relationships with plant roots appear capable of surprisingly long-range dispersals, according to a study appearing online today in Science . (genomeweb.com)
  • At the local level, meanwhile, the team noted that both environmental conditions and spatial distance appeared to affect the types of symbiotic fungi found in plant roots. (genomeweb.com)
  • The fungi form lichens in a symbiotic relationship with one or two photosynthetic partners which may be a cyanobacterium such as Nostoc or a green alga such as Coccomyxa . (eol.org)
  • GO Annotations consist of four mandatory components: a gene product, a term from one of the three Gene Ontology (GO) controlled vocabularies ( Molecular Function , Biological Process , and Cellular Component ), a reference, and an evidence code. (yeastgenome.org)
  • The combination of molecular phylogeny with criteria of geographic and climatic origin showed for the first time in entomopathogenic fungi, that the B. bassiana s. l . can be subdivided into seven clusters with common climate characteristics. (biomedcentral.com)
  • In 2007, autosomal dominant mutations in signal transducer and activator of transcription-3 (STAT3) gene were identified as the molecular cause of this disease. (medscape.com)
  • Interaction annotations are curated by BioGRID and include physical or genetic interactions observed between at least two genes. (yeastgenome.org)
  • Cyphomyrmex is a genus of fungus-growing ants found primarily in South and Central America. (wikipedia.org)
  • 2016. Determining threshold values for barcoding fungi: lessons from Cortinarius (Basidiomycota), a highly diverse and widespread ectomycorrhizal genus. (dannyhaelewaters.com)
  • The entomopathogenic fungi of the genus Beauveria are cosmopolitan with a variety of different insect hosts. (biomedcentral.com)
  • Click "Gene Ontology Details" to view all GO information and evidence for this locus as well as biological processes it shares with other genes. (yeastgenome.org)
  • Peltigerales is an order of lichen -forming fungi belonging to the class Lecanoromycetes in the division Ascomycota . (eol.org)
  • Resolving the evolutionary relationships among Fungi remains challenging because of their highly variable evolutionary rates, and lack of a close phylogenetic outgroup. (biomedcentral.com)
  • Maintaining the effects of nematode-trapping fungi (NTF) agents in order to control plant-parasitic nematodes (PPNs) in different ecological environments has been a major challenge in biological control applications. (encyclopedia.pub)
  • These fungi are taxonomically diverse but traditionally divided into five groups based on the mechanisms by which they attack nematodes: (i) nematode-trapping fungi (NTF), (ii) endoparasitic fungi, (iii) egg- and cyst-parasitic fungi, (iv) toxin-producing fungi, and (v) fungi with special nematode-attacking devices [4]. (encyclopedia.pub)