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GRB2 Adaptor ProteinAdaptor Proteins, Signal TransducingGRB10 Adaptor ProteinAdaptor Proteins, Vesicular TransportGRB7 Adaptor ProteinShc Signaling Adaptor ProteinsAdaptor Protein Complex 2Adaptor Protein Complex 3Adaptor Protein Complex 1src Homology DomainsSignal TransductionMolecular Sequence DataProtein BindingAmino Acid SequenceAdaptor Protein Complex alpha SubunitsProteinsAdaptor Protein Complex beta SubunitsProto-Oncogene Proteins c-crkPhosphorylationAdaptor Protein Complex mu SubunitsPhosphoproteinsAdaptor Protein Complex 4Protein Structure, TertiaryCarrier ProteinsCell LineTyrosineAdaptor Protein Complex gamma SubunitsSon of Sevenless ProteinsClathrinProtein SubunitsBinding SitesAdaptor Protein Complex SubunitsMembrane ProteinsAdaptor Protein Complex delta SubunitsRecombinant Fusion ProteinsMutationMyeloid Differentiation Factor 88CRADD Signaling Adaptor ProteinAmino Acid MotifsIntracellular Signaling Peptides and ProteinsMacromolecular SubstancesSequence Homology, Amino AcidProtein TransportTwo-Hybrid System TechniquesEndocytosisCOS CellsBase SequenceTransfectionProto-Oncogene Proteins c-cblMice, KnockoutPhosphotyrosinePrecipitin TestsImmunoprecipitationProtein-Tyrosine KinasesEnzyme ActivationOncogene ProteinsCrk-Associated Substrate ProteinJurkat CellsRecombinant ProteinsCells, CulturedCloning, MolecularAdaptor Protein Complex sigma SubunitsClathrin-Coated VesiclesHeLa CellsCell MembraneNerve Tissue ProteinsCytoskeletal ProteinsNuclear ProteinsProto-Oncogene ProteinsModels, MolecularHEK293 CellsMonomeric Clathrin Assembly ProteinsReceptors, Interleukin-1src-Family KinasesModels, BiologicalPhospholipase C gammaImmunoblottingBlotting, WesternEndosomesMice, Inbred C57BLCARD Signaling Adaptor ProteinsMutagenesis, Site-DirectedSequence AlignmentGlutathione TransferaseProtein-Serine-Threonine KinasesProtein ConformationPhosphatidylinositol 3-Kinasestrans-Golgi NetworkRNA, Small InterferingProtein Interaction Domains and MotifsKineticsPaxillinUbiquitin-Protein LigasesReceptors, Antigen, T-CellMultiprotein ComplexesCytoplasmDNA, ComplementarySaccharomyces cerevisiaeDNA-Binding ProteinsEscherichia coli
PhosphorylationPI3KMembraneKinaseSignal transductionMoleculesPathwayMediateInteractionELISA KitCytosolGTPaseReceptorsDownstream signalinPhosphataseClathrinPathwaysSerineInteractionsTransmembraneRegulatoryIntracellularLigandComplexBindsComplexesDephosphorylationActinGeneEnzymeHeat shock proteinDetectionAmino acidEpidermalAlphaMacrophageHUMANNeuronalGrowth
Phosphorylation12
  • BTK, a TEC-family tyrosine kinase activated by the B-cell antigen receptor, contains a variety of regulatory domains and it is subject to complex regulation by membrane phospholipids, protein ligands, phosphorylation, and dimerization. (elifesciences.org)
  • Engagement of the TCR by antigenic peptide bound to a histocompatibility molecule (in humans, an HLA molecule) leads to a complex set of biochemical events in which the initial activation of protein phosphorylation is critical. (nih.gov)
  • These complexes contain enzymes that are regulated by protein phosphorylation and whose substrates are located at the membrane. (nih.gov)
  • Initial studies focused on characterization of the multiple TCR subunits and discovery of TCR tyrosine phosphorylation. (nih.gov)
  • Altered membrane events induced selective inhibition of TCR-induced phosphorylation of regulatory P85 subunit of PI3K and AKT as well as abrogated calcium influx. (nature.com)
  • Akt activation leads to the phosphorylation and inactivation of various downstream targets, including glycogen synthase kinase 3 (GSK3), which regulates glycogen synthesis, and the mammalian target of rapamycin (mTOR), which controls protein synthesis and cell growth. (ebiwinner.com)
  • In this study we used phosphorylation of the transcription factor cAMP response element-binding protein as a functional readout to identify cells responding to EGF and FGF-2. (beauty104.com.tw)
  • A significant system of transmitting indicators is certainly by phosphorylation and dephosphorylation of tyrosine residues on essential proteins such as for example development aspect and cytokine receptors adapter proteins and enzymes such as for example non-receptor tyrosine kinases. (biospraysehatalami.com)
  • Phosphorylation creates sites for protein-protein connections between your phosphorylated proteins and signaling substances formulated with SH2 and phosphotyrosine binding domains that recognize phosphotyrosine [13]. (biospraysehatalami.com)
  • Both CaMKKs are partly inhibited by phosphorylation, which in turn triggers adaptor and scaffolding protein 14-3-3 binding. (bvsalud.org)
  • The homopentameric α7-nAChR, abundantly expressed in the brain, is involved in the regulation of the neuronal plasticity and memory and undergoes phosphorylation by protein kinase A (PKA). (bvsalud.org)
  • ROCK inactivates myosin phosphatase through the specific phosphorylation of myosin phosphatase target subunit 1 (MYPT1) at Thr-696, which results in an increase in the phosphorylated content of the 20-kDa myosin light chain (MLC20). (kits-elisa.com)
PI3K4
  • 2016) . A cross-species study of PI3K protein-protein interactions reveals the direct interaction of P85 and SHP2 . (sdbonline.org)
  • A fly-human cross-species comparison of the phosphoinositide-3-kinase (PI3K) interactome was conducted in a Drosophila S2R+ cell line and several NSCLC and human multiple myeloma cell lines to identify conserved interacting proteins to PI3K, a critical signaling regulator of the AKT pathway. (sdbonline.org)
  • These data revealed an unexpected direct binding of Corkscrew, the Drosophila ortholog of the non-receptor protein tyrosine phosphatase type II (SHP2) to the Pi3k21B (p60) regulatory subunit of PI3K (p50/p85 human ortholog) but no association with Pi3k92e , the human ortholog of the p110 catalytic subunit. (sdbonline.org)
  • The key components of the Igf 1 receptor signaling pathway include the IGF-1 receptor, insulin receptor substrate proteins (IRS), phosphatidylinositol 3-kinase (PI3K), AKT, and mammalian target of rapamycin (mTOR). (ebiwinner.com)
Membrane5
  • These initial events lead to the generation of multiple protein complexes that localize at the TCR and the plasma membrane. (nih.gov)
  • LAT, initially characterized by this laboratory, is an integral membrane protein with multiple tyrosine residues. (nih.gov)
  • ATP synthase membrane subunit c loc. (gsea-msigdb.org)
  • ATP synthase membrane subunit f [So. (gsea-msigdb.org)
  • The communication in subunit: enabling lymphoid GT-domains preventing membrane as a subunits addition: A ATM identified to the Faculty of Graduate Studies and Research in isolated pore of the heterotrimers for the use of Master of Nursing. (evakoch.com)
Kinase5
  • A highlight for me was the session organised by Matt Gold (University College London) which included a description of how PTMs are likely to be crucial in the function of alpha2delta auxiliary calcium channel subunits from Annette Dolphin (University College London) and a compelling talk from Moitrayee Bhattacharyya (University of California, Berkeley) on calcium calmodulin-dependent protein kinase II function. (bps.ac.uk)
  • The protein encoded by this gene is a transmembrane glycoprotein that is a member of the protein kinase superfamily. (bioss.com.cn)
  • Ca2+ /CaM-dependent protein kinase kinases 1 and 2 (CaMKK1 and CaMKK2) phosphorylate and enhance the catalytic activity of downstream kinases CaMKI, CaMKIV, and protein kinase B. Accordingly, CaMKK1 and CaMKK2 regulate key physiological and pathological processes, such as tumorigenesis, neuronal morphogenesis, synaptic plasticity, transcription factor activation, and cellular energy homeostasis, and promote cell survival. (bvsalud.org)
  • But these effects are much stronger on CaMKK1 than on CaMKK2 because the CaMKK1:14-3-3γ complex has a more compact and rigid structure in which the active site of the kinase domain directly interacts with the last two C-terminal helices of the 14-3-3γ protein, thereby inhibiting CaMKK1. (bvsalud.org)
  • Therefore, Ca2+ /CaM binding suppression and the interaction of the kinase active site of CaMKK1 with the last two C-terminal helices of 14-3-3γ protein provide the structural basis for 14-3-3-mediated CaMKK1 inhibition. (bvsalud.org)
Signal transduction2
Molecules3
  • Following binding of the antigen-HLA complex to the TCR, receptors aggregate, associated protein tyrosine kinases (PTK) are activated, and TCR subunits and a number of linker or adapter molecules are phosphorylated on tyrosine residues. (nih.gov)
  • Therefore, amino acids in multifunctional docking sites of Met have been exchanged with specific binding motifs for downstream adaptor molecules in order to investigate the signaling potential of the HGF‑Met signaling pathway. (spandidos-publications.com)
  • The Igf 1 receptor signaling pathway regulates cell growth and survival by activating downstream signaling molecules that promote cell proliferation, inhibit apoptosis, and stimulate protein synthesis. (ebiwinner.com)
Pathway4
  • Special mention here to Amy Stainthorp (University of Leeds) who was awarded the best poster prize, sponsored by 2BScientific, for work describing a pathway whereby the adaptor protein Grb2 regulates the FGFR2-mediated miRNA biogenesis process. (bps.ac.uk)
  • Noonan syndrome and related disorders are caused by mutations in genes encoding for proteins of the RAS-ERK1/2 signaling pathway, which affect development by enhanced ERK1/2 activity. (sdbonline.org)
  • This study identified that the protein most commonly affected in Noonan syndrome, the phosphatase SHP2, known in Drosophila as corkscrew (CSW) , controls life span, triglyceride levels, and metabolism without affecting ERK signaling pathway. (sdbonline.org)
  • Further, we driven that LBH589 and KLF9 governed the appearance of pro- and anti- apoptotic protein, suggesting a system which involves the caspase-dependent apoptotic pathway. (immune-source.com)
Mediate2
Interaction3
  • Validation experiments with knockdown of GAB2 and Far-Western blots proved the direct interaction of SHP2 with p85, independent of adaptor proteins and transfected FLAG-p85 provided evidence that SHP2 binding on p85 occurred on the SH2 domains. (sdbonline.org)
  • 5. Mass spectrometry-enabled protein conformational and interaction analysis with emphasis on H/D exchange mass spectrometry. (oregonstate.edu)
  • This download is the types and cells led from a human assembly target content soccer methylated alongside the set of two interaction localizing enzyme proteins in New Zealand. (evakoch.com)
ELISA Kit1
  • Description: A sandwich ELISA kit for detection of GRB2 Related Adaptor Protein 2 from Human in samples from blood, serum, plasma, cell culture fluid and other biological fluids. (therabio.org)
Cytosol1
  • with us one can be cytosol proteins, " badenova( 2009) is four primary photoreceptors, the most non-crossover-generating of which chloroplasts release, phosphorylated, and ready ataxia bacterial to a cascade of challenging glycoprotein GTPases. (evakoch.com)
GTPase4
  • Also directly phosphorylates other proteins like RGS16, activating its GTPase activity and probably coupling the EGF receptor signaling to the G protein-coupled receptor signaling. (bioss.com.cn)
  • Rho GTPase activating protein 24 [Source. (gsea-msigdb.org)
  • Rho GTPase activating protein 20 [S. (gsea-msigdb.org)
  • Rho GTPase activating protein 42 [S. (gsea-msigdb.org)
Receptors4
  • The chemokine signal is transduced by chemokine receptors (G-protein coupled receptors) expressed on the immune cells. (genome.jp)
  • Ras signaling can be thought of in simple terms as occuring downstream of receptor tyrosine kinases, triggered by association of adaptor proteins with the phosphorylated cytoplasmic tails of these receptors. (sdbonline.org)
  • In addition to plexins, various other proteins have been implicated either as receptors for semaphorins or as components of the semaphorin receptor complex ( Pasterkamp and Kolodkin, 2003 ). (rupress.org)
  • Nicotinic acetylcholine receptors (nAChRs) are ligand-gated ion channels composed of five homologous subunits. (bvsalud.org)
Downstream signalin1
  • an adapter protein coupling the receptor to downstream signaling pathways. (bioss.com.cn)
Phosphatase1
  • Noonan syndrome (NS) and NS with multiple lentigines (NSML) cognitive dysfunction are linked to SH2 domain-containing protein tyrosine phosphatase-2 (SHP2) gain-of-function (GoF) and loss-of-function (LoF), respectively. (sdbonline.org)
Clathrin1
Pathways2
  • Activation of protein serine/threonine kinases and pathways coupled to small G proteins, and elevation of intracellular calcium, are examples of the events that follow. (nih.gov)
  • After receptor activation, the alpha- and beta-gamma-subunits of G protein dissociate to activate diverse downstream pathways resulting in cellular polarization and actin reorganization. (genome.jp)
Serine1
Interactions3
  • The study of protein structures and interactions is critical to understand their function. (tuberculosistest.net)
  • Chemical cross-linking of proteins with mass spectrometry (XL-MS) is a rapidly developing structural biology technique able to provide valuable insight into protein conformations and interactions, even as they exist within their native cellular environment. (tuberculosistest.net)
  • We study protein mobility and interactions by solution-phase hydrogen/deuterium exchange mass spectrometry (HDX-MS). (oregonstate.edu)
Transmembrane1
Regulatory1
  • The p85-SHP2 association was validated in human cell lines, and formed a ternary regulatory complex with GRB2-associated-binding protein 2 (human GAB2 but not Drosophila Dos ). (sdbonline.org)
Intracellular1
  • Interferon-γ (IFN-γ)-inducible antimicrobial factors, such as the guanylate binding proteins (GBPs), promote cell-intrinsic defense by attacking intracellular pathogens and by inducing programmed cell death. (bvsalud.org)
Ligand1
Complex3
  • The phosphorylated receptor recruits adapter proteins like GRB2 which in turn activates complex downstream signaling cascades. (bioss.com.cn)
  • COPI coat complex subunit gamma 2 [Sourc. (gsea-msigdb.org)
  • The signaling complex at the cell surface is believed to be a ternary complex formed between two identical FGF ligands, two identical FGFR subunits and either one or two heparan sulfate chains. (beauty104.com.tw)
Binds2
  • EGFR is a cell surface protein that binds to epidermal growth factor. (bioss.com.cn)
  • The ZAP-70 PTK binds to phosphorylated TCR subunits upon receptor activation. (nih.gov)
Complexes1
  • We are currently using a multidisciplinary approach to study LAT as well as the multi-protein complexes that form at phosphorylated LAT. (nih.gov)
Dephosphorylation1
  • After the XPC p16-INK4A and the UV-DDB hemolytic digestion substituted DNA, a separate localization adaptor TFIIH controls identified to the subunit recycling ubiquitin( many) dephosphorylation( Volker et al. (evakoch.com)
Actin1
Gene1
Enzyme3
  • Description: Enzyme-linked immunosorbent assay based on the Double-antibody Sandwich method for detection of Human GRB2 Related Adaptor Protein 2 (GRAP2) in samples from tissue homogenates, cell lysates and other biological fluids with no significant corss-reactivity with analogues from other species. (therabio.org)
  • Description: This is Double-antibody Sandwich Enzyme-linked immunosorbent assay for detection of Human GA Binding Protein Transcription Factor Alpha (GABPa) in serum, plasma, tissue homogenates, cell lysates and other biological fluids. (therabio.org)
  • Description: Enzyme-linked immunosorbent assay based on the Double-antibody Sandwich method for detection of Human GA Binding Protein Transcription Factor Alpha (GABPa) in samples from serum, plasma, tissue homogenates, cell lysates and other biological fluids with no significant corss-reactivity with analogues from other species. (therabio.org)
Heat shock protein1
Detection1
  • WB: For western blot detection of denatured protein samples. (affbiotech.com)
Amino acid1
Epidermal1
  • This protein is a receptor for members of the epidermal growth factor family. (bioss.com.cn)
Alpha1
  • 2003) and is the YAP1 download in the protein of HIF-alpha PPT by dolichol( Berra et al. (evakoch.com)
Macrophage1
  • Macrophage stimulating protein (MSP) was discovered as a serum protein that promoted mouse macrophage motility ( 13 ), and was later purified to homogeneity from human plasma ( 14 ). (spandidos-publications.com)
HUMAN1
  • Complete Evaluation of the Antigenic Influence of Human Papillomavirus Lineage Variation on Recognition by Neutralizing Monoclonal Antibodies Raised Towards Lineage A Main Capsid Proteins of Vaccine-Associated Genotypes Human papillomavirus (HPV) is the causative agent of cervical and different epithelial cancers. (tuberculosistest.net)
Neuronal1
  • Luciferase reporter assays demonstrated that the Adcy1 promoter is selectively activated by neuronal PAS-domain protein 2 (NPAS2)/BMAL1. (jneurosci.org)
Growth2
  • Proteins that act both at the cell surface and intracellularly will be discussed in this thesis, with particular emphasis on the fibroblast growth factors. (beauty104.com.tw)
  • Two generation myocardiocytes, ERCC5( XPG) and the extracellularspace of ERCC1 and ERCC4( XPF), have associated to the free favour subunit to appear the library planning that will migrate the caspase-dependent gamma from the small opening growth( Dunand-Sauthier et al. (evakoch.com)