• CSNK2A2 the casein kinase 2, alpha prime polypeptide contains a protein kinase domain and a catalytic domain. (wikipedia.org)
  • Non-specific serine/threonine protein kinase. (edu.pl)
  • FUNCTIONS IN: protein serine/threonine kinase activity, prote. (riken.jp)
  • CK2 (casein kinase 2) is a serine/threonine-selective protein kinase that has been involved in a variety of cellular processes such as DNA repair, cell cycle control and circadian rhythm regulation. (biomedcentral.com)
  • CK2 (casein kinase 2) is an ubiquitously expressed and highly conserved serine/threonine protein kinase that forms a tetramer containing two catalytic (α and/or α´) subunits and two regulatory β subunits [ 1 ]. (biomedcentral.com)
  • Serine/threonine kinase which acts as an essential component of the MAP kinase signal transduction pathway. (icr.ac.uk)
  • Casein Kinase 2 Alpha 1 (CKII) is a serine/threonine protein kinase with structural activity. (biomedcentral.com)
  • Protein kinase CK2 (Casein kinase II) is an ubiquitous and highly conserved protein serine/threonine kinase that is typically found in tetrameric complexes consisting of two catalytic (alpha and/or alpha\') subunits and two regulatory beta subunits . (proteinkinase.biz)
  • Plays a complex role in regulating the basal catalytic activity of the alpha subunit (By similarity). (ac.be)
  • To increase stability of the holo complex hCK2 holo consist of shortened alpha subunits (aa1-335) and beta subunits. (proteinkinase.biz)
  • Human recombinant casein kinase 2 holo enzyme (hCK2 holo) containing alpha (aa1-335) and beta subunits expressed separately in E. coli as non-fusion proteins, purified by several chromatography steps (HiTrapQ, Heparin agarose, Superdex200) and reconstituted to the CK2 alpha2beta2 holoenzyme in the course of purification. (proteinkinase.biz)
  • Since CK2 alpha is unstable and prone to degradation, CK2 subunits alpha was shortened at the C-terminus to obtain a stable, crystallizable alpha subunit. (proteinkinase.biz)
  • The subunit alpha protein activates transcription of the subunit beta gene" J. Biol. (proteinkinase.biz)
  • A study with the complete set of subunits (alpha, alpha\' and beta), site-directed autophosphorylation mutants and a bicistronically expressed holoenzyme" Eur. (proteinkinase.biz)
  • Battistutta R, Sarno S, De Moliner E, Marin O, Issinger OG, Zanotti G, Pinna LA (2000) "The crystal structure of the complex of Zea mays alpha subunit with a fragment of human beta subunit provides the clue to the architecture of protein kinase CK2 holoenzyme" Eur. (proteinkinase.biz)
  • Furthermore, when used as a heterologous third dose booster, the RBD-Fc vaccines combined with MF59® increased titres of nAb against other variants including alpha, delta, delta+, gamma, lambda, mu, and omicron BA.1, BA.2 and BA.5. (bvsalud.org)
  • In this study, we have used indirect immunofluorescence microscopy and cell fractionation to study the subcellular distribution of all three subunits of chicken CK II, alpha, alpha', and beta. (rupress.org)
  • These results strongly suggest that CK II functions primarily in regulating nuclear activities, and that the two catalytic subunits, alpha and alpha', may carry out overlapping functions. (rupress.org)
  • A series of flavonoid compounds was tested as inhibitors of protein kinase CK2. (nih.gov)
  • Selective inhibitors of protein kinase CK2 with significant cytotoxicity on tumor cells based on a 2-aminothiazole scaffold were described recently. (rcsb.org)
  • Although CK2?2 bears a marked resemblance in amino-acid sequence to CK2?1 (Dahmus et al. (immune-source.com)
  • CK2 is a ubiquitous protein kinase involved in many cell functions. (nih.gov)
  • Profilin 2- ubiquitous actin monomer binding protein. (wikipedia.org)
  • To better understand the function(s) and regulation of this ubiquitous kinase, it is important to know its subcellular distribution. (rupress.org)
  • Inhibition of protein kinase CK2 by anthraquinone-related compounds. (cathdb.info)
  • METHODS: We report on a protein subunit vaccine comprising the receptor-binding domain (RBD) of the ancestral SARS-CoV-2 spike protein, dimerised with an immunoglobulin IgG1 Fc domain. (bvsalud.org)
  • INTERPRETATION: These results demonstrated that an RBD-Fc protein subunit/MF59® adjuvanted vaccine can induce high levels of broadly reactive nAbs, including when used as a booster following prior immunisation of mice with whole ancestral-strain spike vaccines. (bvsalud.org)
  • Particularly in yeast, an organism where gluconeogenesis and glycolytic activity are intermittently coordinated, the multi-subunit GID E3 ligase complex specifically targets the surplus of gluconeogenic enzymes, including the conserved Fructose-1,6-bisphosphatase 1 (Fbp1), for proteasomal degradation. (elifesciences.org)
  • Here, we demonstrate that in vitro casein kinase 2 (CK2) specifically interacts with human 9-1-1 and phosphorylates serines 341 and 387 (Ser-341 and Ser-387) in the C-terminal tail of Rad9. (elsevierpure.com)
  • Chester N, Yu IJ, Marshak DR (1995) "Identification and characterization of protein kinase CKII isoforms in HeLa cells. (proteinkinase.biz)
  • Benitez MJ, Cochet C, Jimenez JS (2001) "A surface plasmon resonance study of the interactions between the component subunits of protein kinase CK2 and two protein substrates, casein and calmodulin" Mol. (proteinkinase.biz)
  • CCDC113 is located on chromosome 16q21 and encodes two distinct isoforms with isoform 2 containing one less alternate in-frame exon compared to the full length protein, isoform 1. (wikipedia.org)
  • and it has been within many diseases especially cancer rendering it an interesting focus on inside CX-6258 manufacture the druggable category of eukaryotic proteins kinases (Pagano et al. (immune-source.com)
  • The GST-removed hCK2?2 proteins which acquired a. (immune-source.com)
  • Casein kinase 2 interacting protein 1 (CKIP1) is a specific interacting protein of the casein kinase 2 (CK2) α subunit, and, by binding CK2 and other proteins, functions as an adaptor to regulate a series of cellular functions. (geneticsmr.com)
  • inhibitors offer beneficial results on nephritis via Ck2?1 inhibition and undesireable effects on spermatogenesis via CK2?2 inhibition (Xu et al. (immune-source.com)
  • is a constitutively active protein kinase and is further fully activated by association with CK2? (immune-source.com)
  • CC04820 exhibited no selectivity with an IC50 of 17?nM for hCK2?1 and an IC50 of 11?nM for hCK2?2. (immune-source.com)
  • Thus we decided the first structure of hCK2?2 complexed with CC04820 in order to clarify the structural differences between hCK2?1 and hCK2?2. (immune-source.com)
  • 2.2 Protein expression and purification Escherichia coli strain HMS174 (DE3) cells (Novagen) were transformed with pGEX-hCK2?2. (immune-source.com)
  • CK2?2-subunit knockout mice for instance create a condition much like globozoospermia in human beings (Xu et al. (immune-source.com)
  • 2 and methods 2.1 Construction of the expression plasmid The coding region corresponding to amino-acid residues Met1-Gln334 of human CK2?2 was amplified by the polymerase chain reaction (PCR) and cloned into the vector pGEX-6P-1 (GE Healthcare) at the restriction sites BamHI-EcoRI providing the construct in a GST-fused form at the N-terminus. (immune-source.com)
  • CKIP1 mRNA expression was detected in 5 human lung cancer cell lines (H-125, H1299, LTEP-A-2, SPC-A-1, and NCL-H446) by semi-quantitative RT-PCR, and in 10 noncancerous lung tissues and 30 non-small lung cancer tissues by real-time quantitative PCR. (geneticsmr.com)
  • Casein kinase II is a predominantly nuclear enzyme. (rupress.org)
  • During mitosis, the bulk of the enzyme was dispersed throughout the cell, though a fraction of all three subunits was associated with the mitotic spindle. (rupress.org)
  • These buildings claim that the prolonged N-terminal portion fixes the activation portion and ?C helix the conformational plasticity which is certainly significant for on/off legislation of enzyme activity in CMGC kinases and thus allows CK2?1 to have constitutive activity. (immune-source.com)
  • Casein kinase 2 (CK2) was one of the first protein kinases to be discovered and has been suggested to be responsible for as much as one-fifth of the eukaryotic phosphoproteome. (ox.ac.uk)
  • 1 Department of Molecular Biology, The John Paul II Catholic University of Lublin, ul. (nih.gov)
  • On the other hand, two other kinase targets, MAPKAPK2/MK2 and MAPKAPK3/MK3, participate in the control of gene expression mostly at the post-transcriptional level, by phosphorylating ZFP36 (tristetraprolin) and ELAVL1, and by regulating EEF2K, which is important for the elongation of mRNA during translation. (icr.ac.uk)
  • However, the comparative transcriptome analysis of two spinach accessions with different bolting times identified genome-wide gene expression profiling and large-scale discovery of flowering-related genes from vegetative and reproductive leaves [ 13 ]. (biomedcentral.com)
  • Deletions of multiple GID subunits compromise cell proliferation, and this defect is accompanied by deregulation of critical cell cycle markers such as the retinoblastoma (Rb) tumor suppressor, phospho-Histone H3 and Cyclin A. We identify the negative regulator of pro-proliferative genes Hbp1 as a bonafide GID/CTLH proteolytic substrate. (elifesciences.org)
  • Loss of CK2α decreased two β-catenin-regulated genes that are involved in GBM-initiating cell growth, OCT4 and NANOG. (stanford.edu)
  • Dobrowolska G, Lozeman FJ, Li D, Krebs EG (1999) "CK2, a protein kinase of the next millennium" Mol. (proteinkinase.biz)
  • In mammals the CK2?2 subunit is certainly highly and solely expressed in the mind and testis helping the idea that CK2?2 provides specific features in these tissue (Guerra Siemer et al. (immune-source.com)
  • We examined primary chick embryo fibroblasts, virally transformed chicken hepatoma cells, as well as HeLa cells transiently transfected with cDNAs encoding chicken CK II subunits. (rupress.org)
  • Binds to a kinase interaction motif within the protein tyrosine phosphatase, PTPRR (By similarity). (icr.ac.uk)
  • Our observations suggest that CK2 plays a crucial role in the ATR-dependent checkpoint pathway through its ability to phosphorylate Ser-341 and Ser-387 of the Rad9 subunit of the 9-1-1 complex. (elsevierpure.com)