• Based on primary sequence comparisons, β subunits are predicted to be modular structures composed of five domains (A-E) that are related to the large family of membrane-associated guanylate kinase proteins. (jneurosci.org)
  • Receptor-stimulated guanine-nucleotide-triphosphate binding to guanine-nucleotide-binding regulatory proteins. (eurekamag.com)
  • The data thus provide evidence for an essential difference between GTP[S] and p[NH]ppG binding to guanine-nucleotide-binding regulatory proteins and suggest that, in addition to the nucleotide-exchange reaction, a (thio)phosphate-group-transfer process via guanine-nucleotide-binding regulatory protein beta subunits is involved in the receptor-stimulated binding of guanine nucleotide triphosphates to guanine-nucleotide-binding regulatory proteins. (eurekamag.com)
  • They accomplish this in huge component by raising cell surface area manifestation of a transportation proteins subunit known as xCT. (woofahs.com)
  • The GTPase-containing subunits of heterotrimeric GTP-binding proteins. (jefferson.edu)
  • This complex attaches (binds) to other proteins called G protein coupled receptors. (medlineplus.gov)
  • However, many enzymes can bind to different combinations of proteins to form groups (or 'complexes') with a variety of three-dimensional shapes, so there may be a variety of enzyme complexes in the solution. (elifesciences.org)
  • containing proteins include the Ran-GTP binding importin and exportin cargo transport proteins. (cellsignal.com)
  • It forms heterodimeric complexes with CORE BINDING FACTOR ALPHA SUBUNITS, and regulates GENETIC TRANSCRIPTION of a variety of GENES involved primarily in CELL DIFFERENTIATION and CELL CYCLE progression. (wakehealth.edu)
  • The observed binding mode is maintained in the context of heterotrimeric influenza polymerase, placing ANP32A in the immediate vicinity of known host-adaptive PB2 mutants. (nature.com)
  • When the protein complex is bound to a receptor, the Gαq protein binds to a molecule called GTP and is turned on (activated). (medlineplus.gov)
  • The encoded protein binds directly to retinoblastoma protein, which regulates cell proliferation. (fishersci.com)
  • Upregulation of xCT, the inducible subunit of a membrane-bound amino acidity transporter, replenishes intracellular glutathione shops to maintain cell viability in an environment of oxidative tension. (woofahs.com)
  • We show that renal cell carcinoma cells and normal renal cells express IL-15 mRNA and membrane-bound IL-15 (MbIL-15). (nih.gov)
  • Multiple membrane-bound heterodisulfide reductase (DsrMK) could promote both energy-conserving and non-energy-conserving menaquinol oxidation. (hindawi.com)
  • Fqo can be hypothesized to be operative during sulfate reduction with CO. The Fqo complex receives electrons from the reduced coenzyme F 420 (F 420 H 2 ), generated from the oxidative acetyl-CoA pathway, and transfers electrons to the membrane-bound respiratory chain by the reduction of menaquinone [ 16 - 18 ]. (hindawi.com)
  • Drosophila Slowpoke (dSlo) calcium-dependent potassium channels bind directly to the catalytic subunit of cAMP-dependent protein kinase (PKAc). (elsevierpure.com)
  • The fundamental question of whether human telomerase catalytic activity requires cooperation across two TERT subunits remains under debate. (elifesciences.org)
  • however, upon binding to guanosine tetraphosphate (ppGpp), the global regulator of stringent response, ObgE exhibits an enhanced interaction with the 50S subunit, resulting in increased equilibrium dissociation of the 70S ribosome into subunits. (rcsb.org)
  • by binding to the 50S subunit of the ribosome, they inhibit bacterial protein synthesis. (msdmanuals.com)
  • Thereafter, receptor-stimulated binding of p[NH]ppG rapidly reached equilibrium, whereas the binding of GTP[S] proceeded further. (eurekamag.com)
  • 1985). 12 Incubate the mixture 10 min at 25C to achieve binding equilibrium. (buyresearchchemicalss.net)
  • 12 Incubate the mixture 10 min in the 20C shaking water bath to achieve binding equilibrium. (buyresearchchemicalss.net)
  • These immundominant allergens were identified by nanoscale UPLC-MS/MS to be 60S ribosomal protein L10 (25 kDa), electron transfer flavoprotein B subunit (29 kDa), ketol acid reductoisomerase (44 kDa), and heat shock protein 70 (65 kDa). (cdc.gov)
  • Each subunit binds one calcium ion (cofactor). (wikipedia.org)
  • It binds as a cofactor to C3b/C4b inactivator (C3bina), which then hydrolyzes the complement fragment C4b. (lu.se)
  • We show here that an interaction of EB1 with the SXIP-containing +TIP CDK5 regulatory subunit-associated protein 2 (CDK5RAP2) regulates several EB1 activities, including microtubule plus-end tracking, dynamics at microtubule plus ends, microtubule and α/β-tubulin binding, and microtubule polymerization. (uky.edu)
  • Subunit composition is a major determinant in high affinity binding of a Ca2+ channel blocker. (aspetjournals.org)
  • High affinity DHP binding to these channels in skeletal muscle membranes has been reported to be independent of Ca2+ addition and to become dependent on Ca2+ after solubilization. (aspetjournals.org)
  • In this study we coexpressed non-alpha 1 components with alpha 1 in L and COS cells and investigated their roles in the regulation of high affinity DHP binding by Mg2+ and Ca2+. (aspetjournals.org)
  • This affinity was 4-fold lower than that of skeletal muscle membrane binding sites (Kd = 0.25 nM). (aspetjournals.org)
  • Coexpression of all of the subunits completely recapitulated the high affinity DHP binding seen with skeletal muscle membranes in the absence of Ca2+ and Mg2+ (Kd = 0.15 nM). (aspetjournals.org)
  • Rat beta 3 subunits expressed in human embryonic kidney 293 cells form high affinity [35S]t-butylbicyclophosphorothionate binding sites modulated by several allosteric ligands of gamma-aminobutyric acid type A receptors. (aspetjournals.org)
  • For the binding affinity of the second, third and fourth ligand, our results suggest pronounced cooperativity in the sequence positive, negative and positive, respectively. (nature.com)
  • Human ANP32A IDD transiently binds to the 627 domain, exploiting multivalency to maximise affinity. (nature.com)
  • Doxycycline has a high degree of lipoid solubility and a low affinity for calcium binding. (rxlist.com)
  • And more importantly, ObgE, as a (p)ppGpp effector, might also have a regulatory role in the production of the 50S subunit and its participation in translation under certain stressed conditions. (rcsb.org)
  • dSlo binds to free PKAc but not to the PKA holoenzyme that includes regulatory subunits and is inactive. (elsevierpure.com)
  • This protein forms a tetrameric complex with the alpha subunit, and stimulates transcription of target genes. (nih.gov)
  • Two series of overlapping peptides comprising the entire sequences of the β-subunits of human lutropin (LH) and choriogonadotropin (hCG) were prepared by a comprehensive synthetic strategy in order to identify all linear regions of the subunit that may participate in binding of the hormone to its receptor. (elsevierpure.com)
  • Each series of peptides (15 residues in length) spanned the entire amino acid sequences of the two β-subunits. (elsevierpure.com)
  • The peptides were tested for their ability to inhibit the binding of 125 I-labeled hCG or LH to rat ovarian membranes and for their ability to inhibit hCG-stimulated progesterone production in a Leydig cell bioassay. (elsevierpure.com)
  • In order to determine important residues that inhibit binding within this region, a third set of peptides was synthesized in which each residue of hCGβ 81-95 was sequentially replaced with the residue l-alanine. (elsevierpure.com)
  • The channels are primarily activated by voltage but are receptors as well, binding the intracellular ligand cyclic AMP. (nature.com)
  • Sample results obtained for [3H]GABA binding to the homomeric subunit GABAA receptors in rats are shown in /em Fig. GABAB, and homomeric subunit GABAA site, formally referred to as the GABAc receptor (Table 1.7.1). (buyresearchchemicalss.net)
  • Prepare homomeric subunit GABAA receptors In 50-ml polypropylene centrifuge tubes, resuspend cerebellar membranes in sufficient 50 mM TrisCl to yield a final concentration of ~8.0 mg protein/ml ARRY-520 R enantiomer using the tissue homogenizer (midpoint setting for ~30 sec). (buyresearchchemicalss.net)
  • Here, using pre-steady state fast kinetics we demonstrate that ObgE is an anti-association factor, which prevents ribosomal subunit association and downstream steps in translation by binding to the 50S subunit. (rcsb.org)
  • Telomerase synthesizes chromosome-capping telomeric repeats using an active site in telomerase reverse transcriptase (TERT) and an integral RNA subunit template. (elifesciences.org)
  • Surprisingly, telomerase reconstitutions yielded heterogeneous DNA-bound TERT monomer and dimer complexes in relative amounts that varied with assembly and purification method. (elifesciences.org)
  • Overall, this work defines human telomerase DNA binding and synthesis properties at single-molecule level and establishes conserved telomerase subunit architecture from single-celled organisms to humans. (elifesciences.org)
  • The channel is a multimeric complex composed of alpha 1, beta, gamma, and alpha 2 delta, of which alpha 1 is the pore-forming and DHP-binding component. (aspetjournals.org)
  • No DHP binding to membranes of cells expressing alpha 1 beta alone was detected in the absence of Ca2+ or Mg2+. (aspetjournals.org)
  • Coexpression of alpha 1 beta with either alpha 2 delta or gamma alone resulted in DHP binding intermediate between levels seen with alpha 1 beta and alpha 1 beta alpha 2 delta gamma. (aspetjournals.org)
  • Thus, this study demonstrates that alpha 2 delta and gamma are essential for full reconstitution of the DHP binding characteristics of the skeletal muscle L-type Ca2+ channel/DHP receptor. (aspetjournals.org)
  • The GTP-Binding protein alpha subunits are grouped into families according to the type of action they have on second messenger systems. (jefferson.edu)
  • Below are the most recent publications written about "GTP-Binding Protein alpha Subunits" by people in Profiles. (jefferson.edu)
  • The GNAQ gene provides instructions for making a protein called guanine nucleotide-binding protein G(q) subunit alpha (Gαq). (medlineplus.gov)
  • Alpha chain binds C4b. (lu.se)
  • The central body of the alpha chain homopolymer supports tentacles, each with the binding site for C4b at the end. (lu.se)
  • Hydrolysis of GTP by the inherent GTPase activity of the subunit causes it to revert to its inactive (heterotrimeric) form. (jefferson.edu)
  • This gene encodes the GA-binding protein transcription factor, beta subunit. (nih.gov)
  • Association of single nucleotide polymorphisms in the nuclear respiratory factor-2 beta subunit-encoding the GABPB1 gene within the occupational environment. (nih.gov)
  • beta, gamma-imino]triphosphate, p[NH]ppG, and the regulation of binding by the formyl-peptide-receptor agonist, fMet-Leu-Phe, were studied in membranes of differentiated HL-60 cells. (eurekamag.com)
  • Core Binding Factor beta Subunit" is a descriptor in the National Library of Medicine's controlled vocabulary thesaurus, MeSH (Medical Subject Headings) . (wakehealth.edu)
  • This graph shows the total number of publications written about "Core Binding Factor beta Subunit" by people in this website by year, and whether "Core Binding Factor beta Subunit" was a major or minor topic of these publications. (wakehealth.edu)
  • Below are the most recent publications written about "Core Binding Factor beta Subunit" by people in Profiles. (wakehealth.edu)
  • In clinical conditions in which an im- beta subunits, and a biologic half-life of 0.5 pairment of the BBB and/or astrocytic hour [5,6]. (who.int)
  • B) Scatchard plot of specific [3H]GABA binding from panel A. Dissociation constant ((Bowery et al. (buyresearchchemicalss.net)
  • Note: In August, 2008, the nomenclature of the GA binding protein transcription factors was clarified. (nih.gov)
  • A non-DNA binding transcription factor that is a subunit of core binding factor. (wakehealth.edu)
  • Activation is dependent on the binding of MelR to four 18 bp sites, centred at position -42.5 (site 2'), position -62.5 (site 2), position -100.5 (site 1) and position -120.5 (site 1') relative to the melAB transcription start point. (birmingham.ac.uk)
  • In order to simplify our analysis, we exploited an engineered derivative of the melAB promoter in which MelR binding to site 2 and site 2', in the absence of CRP, is sufficient for transcription activation. (birmingham.ac.uk)
  • When dissociated from the heterotrimeric complex these subunits interact with a variety of second messenger systems. (jefferson.edu)
  • Here we analyze the complex activation mechanism of homotetrameric HCN2 channels by confocal patch-clamp fluorometry and kinetically quantify all ligand binding steps and closed-open isomerizations of the intermediate states. (nature.com)
  • This complex interaction of the subunits leads to a preferential stabilization of states with zero, two or four ligands and suggests a dimeric organization of the activation process: within the dimers the cooperativity is positive, whereas it is negative between the dimers. (nature.com)
  • In Azotobacter vinelandii, the E1 subunit of the pyruvate dehydrogenase complex binds fpr promoter region DNA and ferredoxin I. (hal.science)
  • Here, we report the identification of the A. vinelandii protein that binds specifically to the paraquat activatable fpr promoter region as the E1 subunit of the pyruvate dehydrogenase complex (PDHE1), a central enzyme in aerobic respiration. (hal.science)
  • In Azotobacter vinelandii, the E1 subunit of the pyruvate dehydrogenase complex binds fpr promoter region DNA and ferredoxin I.. Proceedings of the National Academy of Sciences of the United States of America , 1999, pp.12389-93. (hal.science)
  • In this study, we describe new approaches of subunit labeling for single-molecule imaging, applied to determine the TERT content of complexes assembled in cells or cell extract. (elifesciences.org)
  • It had previously been suggested that TERT is only an active enzyme when it is bound to another TERT molecule, but Wu et al. (elifesciences.org)
  • Secondary outcomes evaluated stress and inflammatory indices using the Perceived Stress Scale (PSS), S100 calcium-binding protein B (S100B), and C-reactive protein (CRP). (nih.gov)
  • We show here that SUR1 modifies the ATP-binding pocket of Kir6.2, by increasing the width of the groove that binds the phosphate tail of ATP, without changing the length of the groove, and by enhancing interaction with the adenine ring. (ox.ac.uk)
  • It interacts preferentially with the underphosphorylated retinoblastoma protein via the E1A-binding pocket B. Three alternatively spliced transcript variants that encode different protein isoforms have been described for this gene. (fishersci.com)
  • In this work, we have studied the binding of MelR to different sites at the melAB promoter, focusing on the orientation of binding of the two MelR HTH motifs, and the juxtaposition of the different bound MelR subunits with respect to each other. (birmingham.ac.uk)
  • Patterns of DNA cleavage after MelR binding were then used to determine the positions of the two HTH motifs at target sites. (birmingham.ac.uk)
  • motifs of Protein Phosphatase 2A PR65/A subunit. (cellsignal.com)
  • The downstream signal transduction toward Eda-A1 involves the canonical Wnt/β-catenin pathway (Clevers 2006 ) and requires lymphoid enhancer-binding factor-1 (Lef-1) for activation of the Ed1 ( Ta ) expression (Durmowicz et al. (springer.com)
  • ATP-sensitive potassium (K(ATP)) channels comprise Kir6.2 and SUR subunits. (ox.ac.uk)
  • Interleukin 22 binding protein (IL-22BP), also known as CRF2-10, CRF2-X, and IL-22 RA2, is a 35-45 kDa secreted glycoprotein in the type II cytokine receptor family (CRF). (rndsystems.com)
  • The most potent inhibitor of LH/ hCG binding was a peptide containing the sequence β81-95, a receptor binding site of the LH/hCG β subunit not previously described. (elsevierpure.com)
  • The N-terminal A domain differs among the four subtypes of Ca 2+ channel β subunits (β 1 -β 4 ) primarily as the result of two duplications of an ancestral gene containing multiple alternatively spliced exons. (jneurosci.org)
  • This region represents a previously unidentified binding site of high inhibitory activity and contains at least 5 residues important for maximal interaction with the LH/ hCG receptor. (elsevierpure.com)
  • Figure 2: Binding of fcAMP to HCN2 channels on concentration jumps. (nature.com)
  • The concentration at which LH/hCG binding was inhibited at 50% (IC 50 ) was 20 μM and 30 μM for hCGβ 81-95 and LHβ 81-95, respectively. (elsevierpure.com)
  • Sequence analysis shows that PDHE1, which was not previously suspected to be a DNA-binding protein, has a helix-turn-helix motif. (hal.science)
  • For fMet-Leu-Phe-stimulated binding of either GTP analogue, a competing nucleotide was required. (eurekamag.com)
  • With GDP as the competing nucleotide, initial rates of fMet-Leu-Phe-stimulated binding of GTP[S] and p[NH]ppG were similar for up to approximately 30 s. (eurekamag.com)
  • However, with GTP[S] as the competing nucleotide, fMet-Leu-Phe reduced the binding of p[NH]ppG, a reaction further enhanced when GDP was additionally present. (eurekamag.com)
  • Adaptive mutants are localised on the C-terminal (627-NLS) domains of the PB2 subunit. (nature.com)
  • Mapping the architecture of the ATP-binding site of the KATP channel subunit Kir6.2. (ox.ac.uk)
  • The site at which ATP binds to mediate K(ATP) channel inhibition lies on Kir6.2, but the potency of block is enhanced by coexpression with SUR1. (ox.ac.uk)
  • To assess the structure of the ATP-binding site on Kir6.2, we used a range of adenine nucleotides as molecular measuring sticks to map the internal dimensions of the binding site. (ox.ac.uk)
  • The GABA binding site formerly designated as GABAC is now recognized as a homomeric subunit GABAA site, rendering the GABAC designation obsolete (http://www.iuphar-db.org/DATABASE/FamilyMenuForward?familyID=72). (buyresearchchemicalss.net)
  • In addition to site β81-95, other sites that inhibited hCG/LH binding but with significantly lower potencies included hCGβ 1-15, LHβ 41-55, and LHβ 91-105. (elsevierpure.com)
  • Activation also depends on the binding of CRP to a single site located between MelR binding site 1 and site 2. (birmingham.ac.uk)
  • Our results show that MelR binds as a direct repeat to site 2 and site 2' with the C-terminal HTH located towards the promoter-proximal end of each site. (birmingham.ac.uk)
  • The orientation in which MelR binds to site 2' appears to be determined by MelR-MelR interactions rather than by MelR-DNA interactions. (birmingham.ac.uk)
  • Epistasis experiments provided evidence that supports the proposed orientation of binding of MelR at its target site. (birmingham.ac.uk)
  • Ca 2+ channel β subunits regulate cell-surface expression and gating of voltage-dependent Ca 2+ channel α1 subunits. (jneurosci.org)
  • 13 Terminate the binding reaction by centrifuging 10 min at 50,000 Be sure to adjust buffer to the proper pH at 20C, as the pH of Tris buffers varies significantly with temperature. (buyresearchchemicalss.net)
  • 13 Terminate the binding reaction by microcentrifuging the samples 5 min at 10,000 em g /em , 20C. (buyresearchchemicalss.net)
  • An SXIP-binding-deficient EB1 mutant displayed significantly lower microtubule plus-end tracking than the wild-type protein in transfected cells. (uky.edu)
  • Finally, for half-maximal receptor-stimulated binding of GTP[S], approximately fivefold higher concentrations of both Mg2+ and GDP were required than for p[NH]ppG binding. (eurekamag.com)
  • 1985). (A) Saturation of specific [3H]GABA binding with increasing concentrations of [3H]GABA. (buyresearchchemicalss.net)
  • The data presented here further show that FdI binds specifically to the DNA-bound PDHE1. (hal.science)
  • Figure 3: Fit of ligand binding and activation by a Markovian state model. (nature.com)