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  • kinetics
  • Additionally, we have characterized the kinetics governing the specificity and discrimination of several widely administered Nucleotide Reverse Transcriptase Inhibitors (NRTI's) used to combat HIV infection including 3TC (Lamivudine), FTC (Emtricitabine), and AZT (Zidovudine) for the wild-type polymerase and mutants with clinical resistance to these compounds. (utexas.edu)
  • cleavage
  • The essential role of hemopexin carboxyl-domain exosites in the cleavage of noncollagenous substrates such as chemokines has also been recently revealed. (springer.com)
  • This article updates a previous review of the role of substrate recognition by MMP exosites in both preparing complex substrates, such as collagen, for cleavage and for tethering noncollagenous substrates to MMPs for more efficient proteolysis. (springer.com)
  • The potential role of collagen binding in regulating MMP-2 (gelatinase A) activation at the cell surface reveals unexpected consequences of substrate interactions that can lead to collagen cleavage and regulation of the activation and activity of downstream proteinases necessary to complete the collagenolytic cascade. (springer.com)
  • Other endoglycosidases, similar to PNGase F, include endoglycosidase F1, endoglycosidase F2, endoglycosidase F3, and endoglycosidase H. These endoglycosidases have more specificity in cleavage and are less sensitive to protein conformation than PNGase F. All of these endoglycosidases, including PNGase F, can be purified from an almond emulsion or flavobacterium meningosepticum. (wikipedia.org)
  • peptides
  • 1991) "Protein Kinase C Substrate and Inhibitor Characteristics of Peptides Derived From the Myristoylated Alanine-Rich C Kinase Substrate (MARCKS) Protein Phosphorylation Site Domain" The Journal of Biological Chemistry266(22):14390-14398. (patentgenius.com)
  • kinetic
  • The substrate specificity was investigated in detail by measuring the relative activity on a range of alternative acceptors, applied in the reverse synthetic reaction, and determination of the kinetic parameters for the best acceptors. (ugent.be)
  • affinity
  • Interestingly, the abrogation of the LeuRS specificity determinant threonine 252 did not improve the affinity of the editing site for the cognate leucine as expected, but instead substantially enhanced the rate of leucyl-tRNA Leu hydrolysis. (elsevier.com)
  • reaction
  • In enzymology, a tryptophan alpha,beta-oxidase (EC 1.3.3.10) is an enzyme that catalyzes the chemical reaction L-tryptophan + O2 ⇌ {\displaystyle \rightleftharpoons } alpha,beta-didehydrotryptophan + H2O2 Thus, the two substrates of this enzyme are L-tryptophan and O2, whereas its two products are alpha,beta-didehydrotryptophan and H2O2. (wikipedia.org)
  • In enzymology, a haloalkane dehalogenase (EC 3.8.1.5) is an enzyme that catalyzes the chemical reaction 1-haloalkane + H2O ⇌ {\displaystyle \rightleftharpoons } a primary alcohol + halide Thus, the two substrates of this enzyme are 1-haloalkane and H2O, whereas its two products are primary alcohol and halide. (wikipedia.org)
  • In enzymology, a trypanothione-disulfide reductase (EC 1.8.1.12) is an enzyme that catalyzes the chemical reaction trypanothione + NADP+ ⇌ {\displaystyle \rightleftharpoons } trypanothione disulfide + NADPH + H+ Thus, the two substrates of this enzyme are trypanothione and NADP+, whereas its 3 products are trypanothione disulfide, NADPH, and H+. (wikipedia.org)
  • In enzymology, a riboflavin kinase (EC 2.7.1.26) is an enzyme that catalyzes the chemical reaction ATP + riboflavin ⇌ {\displaystyle \rightleftharpoons } ADP + FMN Thus, the two substrates of this enzyme are ATP and riboflavin, whereas its two products are ADP and FMN. (wikipedia.org)
  • In enzymology, an estradiol 17beta-dehydrogenase (EC 1.1.1.62) is an enzyme that catalyzes the chemical reaction estradiol-17beta + NAD(P)+ ⇌ {\displaystyle \rightleftharpoons } estrone + NAD(P)H + H+ The 3 substrates of this enzyme are estradiol-17beta, NAD+, and NADP+, whereas its 4 products are estrone, NADH, NADPH, and H+. (wikipedia.org)
  • rightleftharpoons
  • It catalyses the condensation of tryptamine with secologanin to form strictosidine: tryptamine + secologanin ⇌ {\displaystyle \rightleftharpoons } 3-alpha(S)-strictosidine + H2O Thus, the two substrates of this enzyme are tryptamine and secologanin, whereas its two products are 3-alpha(S)-strictosidine and H2O. (wikipedia.org)
  • They catalyse the oxidation of primary amines to aldehydes, with the subsequent release of ammonia and hydrogen peroxide, which requires one copper ion per subunit and topaquinone as cofactor: RCH2NH2 + H2O + O2 ⇌ {\displaystyle \rightleftharpoons } RCHO + NH3 + H2O2 The 3 substrates of this enzyme are primary amines (RCH2NH2), H2O, and O2, whereas its 3 products are RCHO, NH3, and H2O2. (wikipedia.org)
  • role
  • We further established the critical role for the A76 3′-OH group of the tRNA Leu in post-transfer editing, which supports the substrate-assisted deacylation mechanism. (elsevier.com)
  • In doing so, we define the role of induced-fit in nucleotide specificity and mismatch discrimination. (utexas.edu)
  • Studies
  • Substrate specificity studies showed that n -3,6,9 unsaturation was an absolute requirement for conversion of polyunsaturated fatty acids into analogous products containing a cyclopentenone ring. (plantphysiol.org)
  • variety
  • The Walker A motif is best known for its presence in ATP- and GTP-binding proteins, and is also found in a variety of proteins with phosphorylated substrates. (wikipedia.org)
  • family
  • With the objective of improving the differentiation of enzyme specificities in a knowledge-based context, and to obtain new evolutionary insights, we present here a new, robust subfamily classification of family GH5. (diva-portal.org)