• 7. Substrate specificity of the human protein phosphatase 2Cdelta, Wip1. (nih.gov)
  • 8. Determinants for substrate specificity of the bacterial PP2C protein phosphatase tPphA from Thermosynechococcus elongatus. (nih.gov)
  • This is a tyrosine phosphorylated angiotensin II peptide with a substrate sequence specificity for chymase to act. (anaspec.com)
  • Multiple determinants for the high substrate specificity of an angiotensin II-forming chymase from the human heart. (anaspec.com)
  • Antibody staining pattern specificity can be demonstrated with available blocking phosphopeptides. (nih.gov)
  • The phospho-specificity of Phospho-BMAL1 (Ser42) Antibody was verified by peptide blocking using a phosphopeptide or non-phosphopeptide targeting residue Ser42. (cellsignal.jp)
  • To better understand the connection between Wip1 activity and the control of cellular proliferation, we are characterizing the determinants of substrate specificity, identifying novel substrates and determining the functional effects of Wip1 phosphatase activity. (nih.gov)
  • Encouraged by the selective inhibition of cancer cells by a naphthyl-capped phosphopeptide (Nap-ff p y, 1 ), we conjugated the heteroaromatic dipyrrole or tripyrrole motif at the N-terminal of short peptides containing phosphotyrosine or phosphoserine and examined the bioactivity of the resulting phosphopeptides ( 2 - 10 ). (beilstein-journals.org)
  • These results suggest that a heteroaromatic motif at the N-terminal of peptides likely disfavors the formation of extensive nanofibers or morphological changes during enzymatic self-assembly, thus provide useful insights for the development of phosphopeptides as substrates of phosphatases for controlling cell fate. (beilstein-journals.org)
  • One of the most explored EISA processes is the use of alkaline phosphatase (ALP) to convert the micelles made of phosphopeptides to the nanofibers of peptides via enzymatic dephosphorylation [20,60] . (beilstein-journals.org)
  • The facility can do both large- and small-scale peptide synthesis, including the synthesis of phosphopeptides, peptides containing unusual amino acids & multiple antigen peptide systems (MAPS) for vaccine production or monoclonal antibody production. (biosciregister.com)
  • The most studied substrates used to produce bioactive peptides are milk proteins in the form of co-products from dairy industries: caseins, cheese whey, buttermilk, whey protein concentrates and isolates or even pure single proteins that can be obtained at a reasonable price on an industrial scale (e.g. β-lactoglobulin, β-Lg). (intechopen.com)
  • Infrared multiphoton dissociation (IRMPD) and collisionally activated dissociationof peptides in a quadrupole ion trapwith selective IRMPD of phosphopeptides. (anaspec.com)
  • They noted that Ssu72 prefers an unusual substrate: it binds pSer-Pro peptides in a cis configuration, whereas most proline-directed serine phosphatases act on the trans configuration. (stanford.edu)
  • This added ability to separate ions by differences in conformation makes it possible to separate isobaric and isomeric species, such as phosphopeptide positional isomers (i.e., peptides that differ only by the residue that is phosphorylated), which are not easily distinguished by MS techniques alone 6 . (ddw-online.com)
  • It plays an essential role in promoting mitosis and cytokinesis through phosphorylation of a number of different substrates. (rcsb.org)
  • Comparison of thermolytic phosphopeptide maps and determination of the phosphorylated residue by manual Edman degradation identified the main phosphorylation site in intact cells as Ser102. (cornell.edu)
  • DNA harm sets off Cdc25C phosphorylation at serine 216 offering the energetic binding site for 14-3-3 (8 17 33 38 44 52 Binding of T-705 14-3-3 towards the phosphorylated Cdc25C causes translocation from the complicated in to the cytoplasm separating the Cdc25C phosphatase from its nuclear substrates (28 41 52 such as for example Cdc2. (sciencepop.org)
  • Approximately 30% of human proteins were previously estimated to be phosphorylated, but researchers at the Laboratory of Molecular and Cellular BioAnalysis at Kyoto University developed a highly selective enrichment method for phosphopeptides which, when applied to proteome-wide acquisition of cellular phosphorylation status, revealed that at least 70% of human proteins are phosphorylated. (ddw-online.com)
  • PRAS40 (proline-rich Akt/PKB substrate of 40 kDa) acts at the intersection of the Akt- and mTOR-mediated signaling pathways and its phosphorylation by Akt and by mTORC1 results in dissociation of PRAS40 from mTORC1 which may relieve an inhibitory constraint on mTORC1 activity (Wiza et al. (phosphosolutions.com)
  • The protein encoded serves as a substrate for several different protein kinases and phosphorylation may function in the regulation of this protein in the nerve terminal. (arigobio.cn)
  • While ALP-catalyzed EISA has received considerable exploration, the structures of the peptide substrates mainly have centered on naphthylacetyl-capped phosphopeptides (e.g. (beilstein-journals.org)
  • 4. Development of a substrate-based cyclic phosphopeptide inhibitor of protein phosphatase 2Cdelta, Wip1. (nih.gov)
  • 9. The Wip1 phosphatase PPM1D dephosphorylates SQ/TQ motifs in checkpoint substrates phosphorylated by PI3K-like kinases. (nih.gov)
  • 14. Substrate-dependent metal preference of PPM1H, a cancer-associated protein phosphatase 2C: comparison with other family members. (nih.gov)
  • 18. Probing protein phosphatase substrate binding: affinity pull-down of ILKAP phosphatase 2C with phosphopeptides. (nih.gov)
  • [ 9 ] Because the vast majority of mutations reside in and around the broad intramolecular interaction surface between the N-SH2 and PTP domains of the PTPN11 protein, they have been suggested to affect the intramolecular N-SH2/PTP binding in the absence of a phosphopeptide, leading to excessive phosphatase activities. (medscape.com)
  • Purified bovine DARPP-32 was phosphorylated in vitro by casein kinase II to a stoichiometry greater than 2 mol of phosphate/mol of protein whereas two structurally and functionally related proteins, protein phosphatase inhibitor-1 and G-substrate, were poor substrates for this enzyme. (cornell.edu)
  • Antibodies were produced by immunizing rabbits with KLH-conjugated synthetic phosphopeptide. (arigobio.cn)
  • This precompiled information serves as a substrate and matrix to embed your contributions, but it is by no means the final word - Homo sapiens can do much better! (wikigenes.org)
  • In in vitro kinetic experiments measuring OGT and OGA activity on a panel of protein substrates, kinetic parameters for OGT were shown to be variable between various proteins while kinetic parameters for OGA were relatively constant between various proteins. (wikipedia.org)
  • Merck selected one biomarker, generated a high-quality antibody reagent against that phosphopeptide, and then showed that this biomarker positively correlates with PIK3 pathway activation and predicts AKT inhibitor sensitivity," he said. (genomeweb.com)
  • Protein ubiquitylation is a post-translational modification (PTM) process that covalently modifies a protein substrate with either mono-ubiquitin moieties or poly-ubiquitin chains often at the lysine residues. (nsf.gov)
  • I summarize the methods utilized in defining the ubiquitylation machinery by bioinformatics, identifying ubiquitylation substrates by proteomics, and characterizing the ubiquitin E3 ligase-substrate pathways by functional genomics. (nsf.gov)
  • Degradation of a protein via the ubiquitin pathway proceeds by two discrete and successive steps: (a) covalent attachment of multiple ubiquitin molecules to the protein substrate, and (b) degradation of the targeted protein by the 26S proteasome complex. (justia.com)
  • For OGT, studies have shown that substrate recognition is regulated by a number of factors including aspartate and asparagine ladder motifs in the lumen of the superhelical TPR domain, active site residues, and adaptor proteins. (wikipedia.org)
  • basic kinase-substrate motifs are common with 55 human protein kinases recognizing an Arg at a position −3 from the phosphorylated Ser, whilst the Arg ( i− 3) Ser i Lys ( i +4) is a motif found in over 2000 human proteins. (portlandpress.com)
  • Examining prostate cancer cells treated with three investigational drugs targeting the phosphatidylinositol 3-kinase, or PI3K, signaling pathway, the researchers identified 375 nonredundant serine-threonine phosphopeptides within the pathway, of which 71 were regulated by at least one drug and 11 were regulated by all three drugs. (genomeweb.com)
  • Sequencing of chymotryptic and thermolytic phosphopeptides from bovine DARPP-32 phosphorylated by casein kinase II suggested that the main phosphorylated residues were Ser45 and Ser102. (cornell.edu)
  • Known substrates for the TRK receptors are SHC1, PI-3 kinase, and PLC-gamma-1. (immunoway.com)
  • Prepared from pooled rabbit serum by affinity purification via sequential chromatography on phospho and non-phosphopeptide affinity columns. (phosphosolutions.com)
  • Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. (arigobio.cn)
  • The team selected phospho-PRAS40 Thr246 , which had been the phosphoprotein most highly regulated by the AKT and PI-103 inhibitors and is a direct substrate for AKT. (genomeweb.com)
  • Some members of the serpin family may be substrates rather than inhibitors of SERINE ENDOPEPTIDASES . (nih.gov)
  • grubii grown in culture led to the identification of 1089 phosphopeptides derived from 648 proteins including about 45 kinases. (johnshopkins.edu)
  • Efficient methods of protein extraction and phosphopeptide purification are required to ensure the detection of high quality of proteins. (bio-thing.cn)
  • Motif enrichment analysis revealed that most CDK family substrates were found to be phosphorylated. (johnshopkins.edu)
  • As crystal structures have shown that OGT requires its substrate to be in an extended conformation, it has been proposed that OGT has a preference for flexible substrates. (wikipedia.org)
  • During the first years of the 1990s I made an attempt with Johan Rosén to look for additional modifications of phosphopeptides, which led to the rediscovery of prolyl endopeptidase and the discovery of its preference for a phosphorylated substrate. (uu.se)
  • To investigate the potential feasibility of using these phosphopeptides as biomarkers for PIK3 pathway inhibition, the scientists then moved to an experiment designed to demonstrate "proof-of-concept for clinical translation," Hendrickson said. (genomeweb.com)
  • The O-GlcNAc modification is installed by OGT in a sequential bi-bi mechanism where the donor sugar, UDP-GlcNAc, binds to OGT first followed by the substrate protein. (wikipedia.org)
  • The O-GlcNAc modification is removed by OGA in a hydrolysis mechanism involving anchimeric assistance (substrate-assisted catalysis) to yield the unmodified protein and GlcNAc. (wikipedia.org)
  • They then enriched for phosphopeptides using phospho-motif-specific antibodies and sequenced them via electron disassociation mass spectrometry on a Thermo Electron triple quadrupole-ETD instrument. (genomeweb.com)
  • In addition, non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide. (arigobio.cn)
  • While crystal structures have been reported for both OGT and OGA, the exact mechanisms by which OGT and OGA recognize substrates have not been completely elucidated. (wikipedia.org)
  • We determined the amino acid and radiolabel sequences of tryptic [32P]phosphopeptides of the purified human estrogen receptor (hER) from MCF-7 cells and Sf9 cells. (nih.gov)
  • Further studies in which I participated led to the first identification of a consensus amino acid sequence around the phosphorylatable serine of a PKA‑substrate. (uu.se)
  • Cellular FLICE-like inhibitory protein (cFLIP) is a crucial regulator of cell death and a substrate of Caspase-8. (sfb1530.de)
  • The catalytic activity of some of them is quite specific and the composition of the hydrolysate is predictable when substrates are quite pure [ 1 ]. (intechopen.com)
  • In 2006, Hanna et al found that Noonan syndrome mutations enhance SHP-2 catalytic activity, whereas the activity of representative LS mutants is undetectable when assayed using a standard PTP substrate. (medscape.com)
  • ATR, is phosphorylated at threonine 1989 site (T1989) in response to DNA damage and ATR activation leads to activation of downstream substrates, signaling cascades and cell cycle arrest. (nih.gov)
  • Well established methods involving the analysis of 32 P-labeled phosphoproteins by Edman degradation and two-dimensional phosphopeptide mapping have proven to be powerful but not without limitations. (biomedcentral.com)
  • This analysis yielded 375 non-redundant phosphopeptides tied to the PI3K pathway, a number Hendrickson said was somewhat higher than the researchers had expected, particularly given "that the PIK3 pathway has been so well studied to date. (genomeweb.com)
  • In the first day period analysis without treatment showed the highest values of ΔQ (final value of substrate´s mineral loss) while the lowest values were obtained in the G2 and G3 groups, which did not presented statistical difference among them. (bvsalud.org)
  • Many of the products and product lines released this year were based on recent, but established technologies, such as superficially porous particle (SPP) substrates. (chromatographyonline.com)