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  • suitability
  • The suitability of enzymes as markers is not only determined by their great molar activity that is to be largely maintained even after binding to immunoreactants, their stability and commercial availability but especially by their simple, practicable and sensitive detectability. (springer.com)
  • In this section, we describe the characteristics of our enzyme substrates and the fluorophores and chromophores from which they are derived, focusing primarily on the suitability of these substrates for different types of enzyme assays. (thermofisher.com)
  • Fluorescent
  • These fluorescent products can typically be quantitated in the presence of the unreacted substrate using a fluorometer or a fluorescence microplate reader. (thermofisher.com)
  • Detecting Enzymes That Metabolize Phosphates and Polyphosphates-Section 10.3 ), are only weakly fluorescent in the optimal configuration for 7-hydroxy-4-methylcoumarin detection (excitation/emission = 360/460 nm, Figure 10.1.2 ). (thermofisher.com)
  • wavelength
  • Peptidic FRET (Fluorescence Resonance Energy Transfer) substrates contain a donor (a fluorophore) and an acceptor (quencher) which absorbs at the emission wavelength of the fluorophore. (bachem.com)
  • Can I search my dye labeled FRET substrates by wavelength? (bachem.com)
  • Fortunately, many substrates have low intrinsic fluorescence or are metabolized to products that have longer-wavelength excitation or emission spectra ( Figure 10.1.1 ). (thermofisher.com)
  • The term "P450" is derived from the spectrophotometric peak at the wavelength of the absorption maximum of the enzyme (450 nm) when it is in the reduced state and complexed with carbon monoxide. (wikipedia.org)
  • mechanism
  • The presence of a small concentration of dimethyl sulfoxide destabilized the dimer and changed the unfolding mechanism of the enzyme. (jbsdonline.com)
  • The NAD + synthetase conformation of the homodimer with partially dissociated dimer interface can be important from the point of view of an inhibition mechanism study of this dimeric enzyme. (jbsdonline.com)
  • catalytic efficiency
  • In general, the catalytic efficiency ( k cat /K m ) toward small hydrophobic substrates was increased (up to 16 times) by hydrophobic substitutions at position 166 in the binding cleft. (sciencemag.org)
  • Exceeding the optimal binding volume of the cleft (∼160 Å 3 ), by enlarging either the substrate side chain or the side chain at position 166, evoked precipitous drops in catalytic efficiency ( k cat /K m ) (up to 5000 times) as a result of steric hindrance. (sciencemag.org)
  • homology
  • The second model was a homology model for YOL151w based on the enzyme crystal structure of Sporobolomyces salmonicolor carbonyl reductase, SSCR. (hindawi.com)
  • molecule
  • Substrate binding induces a change in the conformation of the active site, often displacing a water molecule from the distal axial coordination position of the heme iron, and changing the state of the heme iron from low-spin to high-spin. (wikipedia.org)
  • spectral
  • Many of our substrates have unique spectral profiles that may be useful in the co-localization of antigens using spectral imaging systems. (vectorlabs.com)
  • heat resistant
  • Substrates that are designated "heat resistant" were developed on tissue then subjected to heat induced epitope retrival (HIER) using a pressure cooker technique (stained tissue was pressure cooked for 1 minute in Antigen Unmasking Solution, returned to room temperature, and rinsed in buffer). (vectorlabs.com)
  • tissue
  • These proprietary substrates provide novel color choices essential for staining antigens in pigmented tissues and melanomas as well as for localizing multiple antigens in the same tissue section. (vectorlabs.com)
  • suitable
  • Entering the enzyme name in the search function could already return you a number of suitable substrates. (bachem.com)
  • Chandler, H.M., and Hurell, G.R., 1982, A new enzyme immunoassay system suitable for field use and its application in a snake venom detection kit, Clin. (springer.com)
  • Size
  • As a result, predicting stereoselectivity of KREDs is more complicated than is allowed for with just substrate size and geometry. (hindawi.com)
  • method
  • Overview of the enzyme activity profiling method. (thermofisher.com)
  • A conserved glycine (Gly 166 ), located at the bottom of the substrate binding left, was replaced by 12 nonionic amino acids by the cassette mutagenesis method. (sciencemag.org)
  • For SSCR the molecular modeling proved to be a better method for predicting stereoselectivity of the enzymes. (hindawi.com)