• These mitochondrial responses are governed by general principles of regulatory biology exemplified by changes in gene expression, protein translation, protein complex formation, transmembrane transport, enzymatic activities and metabolite levels. (nature.com)
  • Mice lacking mitochondrial uncoupling protein are cold-sensitive but not obese. (nature.com)
  • As noted above, the AE or solute carrier 4 (SLC4) family includes 10 members (SLC4A1-5, SLC4A7-11), which are expressed in multiple tissues in the human body. (tcdb.org)
  • Structural analysis of integral membrane proteins, which comprise a large proportion of druggable targets and pose particular challenges for X-ray crystallography, by cryo-EM has enabled insights into important drug target families such as G protein-coupled receptors (GPCRs), ion channels, and solute carrier (SLCs) proteins. (silverchair.com)
  • MFS is subgrouped into 74 subfamilies based on phylogenetic analysis, postulated substrate specificity and expected transport mode (Madej M.G, Advances in Biology, Hindawi, Volume 2014, Article ID 523591:1-20). (embl.de)
  • Structures of macromolecular drug targets, especially when bound to modulators, can reveal ligand-binding pockets and pinpoint protein-ligand interactions that influence the mechanism of action, potency, and specificity. (silverchair.com)
  • M. tuberculosis also possesses a second essential PPTase called TB-AcpS, which has very narrow substrate specificity and cannot post-translationally modify BpsA. (wgtn.ac.nz)
  • In an attempt to widen the substrate specificity a combination of rational engineering and directed evolution was employed. (wgtn.ac.nz)
  • Intuitively, one would account substrate specificity of the condensing enzyme responsible for translocation, e.g., by the condensing enzyme of the same module preventing the elongated polyketide from re-binding, but several studies demonstrated that the substrate specificity is rather weak and cannot meet this demand (e.g. ref 7 ). (nature.com)
  • BirA biotinylates via the lysine side chain of biotin-accepting proteins/peptides, including natural substrate, carboxyl carrier protein (BCCP),and Avi Tag fusion proteins. (watson-int.cn)
  • The α-subunits of the oxaloacetate and methylmalonyl-CoA decarboxylases are homologous to many biotin-containing enzymes including (1) pyruvate carboxylases, (2) homocitrate synthases, (3) biotin carboxyl carrier proteins, (4) isopropylmalate synthases and (5) acyl-CoA carboxylase. (tcdb.org)
  • During my research work on membrane transport proteins, I have established a number of techniques for studying membrane protein structure-function, including FRET and blue-native polyacrylamide gel electrophoresis. (wayne.edu)
  • The animal AE proteins consist of homodimeric complexes of integral membrane proteins that vary in size from about 900 amino acyl residues to about 1250 residues. (tcdb.org)
  • Functional reintegration into lipid environments represents a major challenge for in vitro investigation of integral membrane proteins (IMPs). (tudelft.nl)
  • Since that enzyme lacks a thioesterase domain, release of the dihydromonacolin L acid moiety from the polyketide synthase requires this dedicated enzyme. (genome.jp)
  • The enzyme catalyzes an essential step in fatty acid biosynthesis, the reduction of the 2,3-double bond in enoyl-acyl-[acyl-carrier- protein] derivatives of the elongating fatty acid moiety. (cathdb.info)
  • The N-terminal domain binds the glutaconyl-CoA, and the C-terminal domain binds the biotinyl lysine moiety. (tcdb.org)
  • For functionality, NRPSs require the attachment of a phosphopantetheine moiety to their peptidyl carrier protein domains. (wgtn.ac.nz)
  • BpsA contains an adenylation domain that recognises and sequentially binds two molecules of L-glutamine, an oxidation domain that is believed to oxidise each glutamine monomer, a peptidyl carrier protein domain that binds the phosphopantetheine moiety, and a thioesterase domain that cyclises each glutamine and releases the final bicyclic product from the enzyme. (wgtn.ac.nz)
  • Mupirocin is able to bind to bacterial and archaeal versions of the enzyme, but not eukaryotic versions. (wikipedia.org)
  • Nasu, S., Wicks, F.D. and Gholson, R.K. L -Aspartate oxidase, a newly discovered enzyme of Escherichia coli , is the B protein of quinolinate synthetase. (enzyme-database.org)
  • MftC is a radical S-adenosylmethionine (SAM) enzyme that may function to modify mycofactocin, a conserved polypeptide that might serve as an electron carrier. (nih.gov)
  • The enzyme from the bacterium Escherichia coli accepts substrates with carbon chain length from 4 to 18. (cathdb.info)
  • The FAS-I enzyme from the bacterium Mycobacterium tuberculosis prefers substrates with carbon chain length from 12 to 24 carbons. (cathdb.info)
  • The enzyme transfers CO 2 from glutaconyl-CoA to a biotin carrier protein (the γ-subunit) that is subsequently decarboxylated by the carboxybiotin decarboxylation site within the Na + pumping beta subunit (Gcdβ). (tcdb.org)
  • The D4 domains of vWF, which dock to the two CUB domains and to the TSP-8 domain of ADAMTS13, help to open the enzyme and thus expose its otherwise cryptic exosite 3. (esanum.com)
  • Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam ). (lbl.gov)
  • tRNA thiolation factors Urm1?Uba4 represent the most ancestral known ubiquitin?like protein/E1 enzyme conjugation system, whose structural mechanisms have however remained elusive. (fnp.org.pl)
  • BirA functions both as a DNA-binding protein that represses the biotin biosynthesis operon as well as an enzyme that synthesizes its own corepressor, bio-5′-AMP, an intermediate in biotinylation reactions. (watson-int.cn)
  • Antibiotic biosynthesis monooxygenase domain [InterProScan]. (ntu.edu.sg)
  • While the polyketides are extraordinarily diverse, they are produced by evolutionarily related proteins that share the molecular logic for polyketide biosynthesis 4 . (nature.com)
  • I am a plant cell biologist and protein biochemist at Oxford Brookes University with expertise in the structure and function of the plant endoplasmic reticulum (ER), membrane proteins and auxin biosynthesis using biochemical techniques as well as high-resolution live cell imaging. (brookes.ac.uk)
  • My additional independent research on ER localisation and splicing in auxin biosynthesis showed for the first time ER-localisation for an auxin biosynthetic protein. (brookes.ac.uk)
  • My research interest has been on structural and functional studies on physiologically and pharmacologically important folate membrane transporters, including human reduced folate carrier (hRFC) and the human proton-coupled folate transporter (hPCFT). (wayne.edu)
  • Along the line, I am interested in structure/function studies on folate transporters, including reduced folate carrier and proton coupled folate transporter. (wayne.edu)
  • The major facilitator superfamily (MFS) of membrane proteins represents the largest family of secondary transporters with members from Archaea to Homo sapiens. (embl.de)
  • The sequences identity ranges around 12-18% but regions of functional similarity (e.g., substrate- or H-binding sites) align for only very closely related MFS transporters. (embl.de)
  • Two recently identified exo-lytic N -acetylmuramidases (Tf_NamZ1 and Tf_NamZ2) specifically cleave the peptide-free, exogenous (nutrition source) PGN in the periplasm and release the MurNAc and disaccharide substrates for the transporters Tf_MurT and Tf_AmpG, respectively, whereas the peptide-containing, endogenous (the self-cell wall) PGN stays unattached. (karger.com)
  • ACE-2 is the SARS-CoV and SARS-CoV2 Spike protein receptor in vivo (4-6), functions catalytically as a carboxypeptidase to cleave several substrates including angiotensins I and II, and acts as a partner for B0AT1-family amino acid transporters (1, 2). (rndsystems.com)
  • Before entering lipid synthesis pathways, different kinds of acyl-CoA transporters which acts as the major carrier of acyl-CoAs, such as fatty acid binding protein (FABP), sterol carrier protein 2 (SCP2) and acyl-CoA-binding protein (ACBP), would activate and convert fatty acids to fatty acyl-CoA esters via a reaction catalyzed by fatty acyl-CoA synthetase and transported to various metabolic locations [ 11 ]. (biomedcentral.com)
  • The actions of the proteins that control the uptake, storage, and distribution of zinc, the zinc transporters, are under intense investigation due to their emerging role in type 2 diabetes. (hindawi.com)
  • We demonstrate that the carboxy-terminal domain is crucial for the catalytic activity and ferroptosis inhibition of FSP1 by mediating the functional dimerization of FSP1, and the formation of two active sites located on two sides of FAD, which are responsible for ubiquinone reduction and a unique FAD hydroxylation respectively. (bvsalud.org)
  • The study of CylC-like enzymes will provide insights into substrate scope, mechanism and catalytic partners, and will also enable engineering these biocatalysts for similar or additional C-H activating functions. (biomedcentral.com)
  • This has been proposed by the ε subunit taking an extended conformation, with a terminal helix probing into the central architecture of the hexameric catalytic domain, preventing ATP hydrolysis. (tudelft.nl)
  • BirA, a member of the group II biotin-protein ligase family, contains an N-terminal helix-turn-helix DNA-binding domain, a catalytic core that catalyzes biotinyl 5′ adenylate (bio-5′-AMP) synthesis, and a C-terminal domain that plays a role in DNA binding, dimerization, and catalytic function. (watson-int.cn)
  • Catalytic domains are represented by different colors, as indicated. (nature.com)
  • BirA, the biotin-protein ligase (BPL) of Escherichia coli, is also known as biotin operon repressor, biotin-[acetyl-CoA-carboxylase] ligase, and biotin-[acetyl-CoAcarboxylase] synthetase. (watson-int.cn)
  • We have identified a cytosolic acyl-CoA binding protein, with highly conserved amino acid residues and a typical acyl-CoA binding domain in N. caninum . (biomedcentral.com)
  • AE1 and NBCe1), and (iii) may provide sites for regulation of transporter function via protein kinase A phosphorylation (e.g. (tcdb.org)
  • In the poster section, Joanna Shisler (University of Illinois at Urbana-Champaign [UIUC], Urbana) reported that the modified virus, Ankara, activates nuclear factor κB through the mitogen-activated protein kinase, extracellular signal-regulated kinase (MEK)/extracellular signal-regulated kinase (ERK) pathway, possibly facilitating the host immune response. (cdc.gov)
  • In the poster section, Joanna Shisler (University of Illinois at Urbana-Champaign [UIUC], Urbana) reported that the modified virus, Ankara, activates nuclear factor κB through the mitogen-activated protein kinase, extracellular signal–regulated kinase (MEK)/extracellular signal–regulated kinase (ERK) pathway, possibly facilitating the host immune response. (cdc.gov)
  • Using a wide range of state-of-art biochemical technologies including scanning cysteine mutagenesis and cysteine-scanning accessibility methods (SCAM), the important amino acid residues involved in hRFC function and substrate binding were dissected. (wayne.edu)
  • A hydrophobic amino acid content of 60-70% of most MFS members, high alfa-helix content and an inherent symmetry of the proteins with regard to helix kinks and bends provides nonspecific overlapping of residues and probably accounts for the reported similarities. (embl.de)
  • Conserved residues therein, D203 (IIIa), Y229 (IV) and N373, G377, S382 and R389 (VIII), provide Na + binding sites and the translocation pathway. (tcdb.org)
  • Our experiments show that all IutY mutant proteins that contain periplasmic residues depend on RseP for activation. (vu.nl)
  • A creatine-driven substrate cycle enhances energy expenditure and thermogenesis in beige fat. (nature.com)
  • Local attraction of substrates and co-substrates enhances weak acid and base transmembrane transport, and principles in the attraction and facilitation of weak acid and base transport substrates, including monocarboxylates, ammonium, bicarbonate, and arsenite, plus protons as a co-substrate have been elucidated ( Epalle and Beitz 2022 ). (tcdb.org)
  • Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. (rndsystems.com)
  • Here it was shown that mixing BpsA, a PPTase, the enzymatic substrates, and a potential inhibitor enables screening for PPTase inhibition by monitoring the rate or extent of indigoidine synthesis. (wgtn.ac.nz)
  • The crystal structure of the Uba4-Urm1 complex provides molecular insights into substrate recognition and the enzymatic reaction cascade. (fnp.org.pl)
  • and a C-terminal RING finger domain, a motif often found in ubiquitin-protein isopeptide ligases (E3). (nih.gov)
  • However, if either of these processes proceeds too slowly or fails, HSP70/HSPA1A can interact with the HSP40 co-chaperone protein and the CHIP/STUB1 Ubiquitin ligase (E3) to promote ubiquitination and degradation of the nascent polypeptide or denatured protein (3,4). (novusbio.com)
  • An international team of researchers from the Max Planck Research Group at the Malopolska Centre of Biotechnology (MCB) deciphered the molecular mechanism of a sulfur carrier system that stands at the root of all eukaryotic Ubiquitin-conjugation systems. (fnp.org.pl)
  • The team obtained the first three-dimensional atomic model of the E1-like sulfurtransferase, Uba4, with its bound substrate, the Ubiquitin-like protein (UBL), Urm1. (fnp.org.pl)
  • The discovery of the Ubiquitin-mediated proteolysis has received the Nobel Prize in 2004 and involves thousands of cellular proteins. (fnp.org.pl)
  • Gene/protein expression and cytokine production were investigated too. (bvsalud.org)
  • The fatty acid desaturases domain was the essential domain of the fatty acid desaturases gene family. (springeropen.com)
  • As an example, Wood and colleagues (pp. 2695-2710) present the sialic acid gene repressor NanR (PDB-6WFG), where cryo-EM revealed the DNA-binding mode. (silverchair.com)
  • In humans, the CRYAA gene encodes a 173 amino acid residue protein by single copy genes located on chromosome 21. (molvis.org)
  • Posttranscriptional regulation of the human reduced folate carrier as a novel adaptive mechanism in response to folate excess or deficiency. (wayne.edu)
  • 9. Hou, Z., and Matherly, L.H., 2009, Oligomeric Structure of the Human Reduced Folate Carrier: Identification of Homo-oligomers and Dominat-negative Effects on Carrier Expression and Function. (wayne.edu)
  • Radical SAM enzymes are characterized by a conserved CxxxCxxC motif, which coordinates the conserved iron-sulfur cluster that is involved in the reductive cleavage of SAM and generates a 5'-deoxyadenosyl radical, which in turn abstracts a hydrogen from the appropriately positioned carbon atom of the substrate. (nih.gov)
  • Radical SAM enzymes with a C-terminal SPASM domain contain at least one other iron-sulfur cluster. (nih.gov)
  • The selectivity with which halogenating enzymes act on their substrates renders halogenases interesting targets for biocatalyst development. (biomedcentral.com)
  • These enzymes are found in a variety of biosynthetic contexts, which include fatty-acid activating enzymes, type I or type III polyketide synthases, dialkylresorcinol-generating enzymes, monooxygenases or Rieske proteins. (biomedcentral.com)
  • But did you know that molecules can run along linear assemblies of multi-domain enzymes? (nature.com)
  • The CusB protein is part of the CusCBA periplasmic Cu(I) efflux system in Gram-negative bacteria, and it was recently reported to play a key role in the functioning of the whole CusCBA system, in which conformational changes as well as the assembly/disassembly process control the opening of the transporter. (biorxiv.org)
  • It undergoes conformational changes within the A1 domain and through the development of the A2 domain. (esanum.com)
  • The membrane-bound fatty acid desaturases, which include Δ5-, Δ6-, Δ9-, Δ12- and Δ15-desaturase in the mammals, fungi, insects, higher plants and cyanobacteria, use fatty acids esterified to complex lipid as the substrate, and use cytochrome (cyt) b5 oxidoreductase and cyt b5 as electron donors. (springeropen.com)
  • Lipid synthesis requires bulk carbon skeleton acyl-CoAs, the transport of which depends on the acyl-CoA binding protein (ACBP). (biomedcentral.com)
  • Alters lipid metabolism by interacting with hepatocellular proteins involved in lipid accumulation and storage (PubMed:14602201). (proteopedia.org)
  • Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors. (novusbio.com)
  • Heat Shock 70kDa Protein (HSP70), also known as Heat Shock 70kDa Protein 1A (HSPA1A), is a 641 amino acid (aa) member of the HSP70 family of molecular chaperones with a predicted molecular weight of 70 kDa. (novusbio.com)
  • The lead author, Marta Pabis, explains that ?UBL protein modifications in eukaryotes might have evolved from prokaryotic sulfurtransferase systems and our study provides further evidence that the Uba4-Urm1 system represents that missing ?molecular fossil? (fnp.org.pl)
  • It has an N-terminal nucleotide-binding domain, which contains ATPase activity, and a C-terminal substrate-binding domain (1). (novusbio.com)
  • Participates in the viral particle production as a result of its interaction with the non-structural protein 5A (By similarity). (proteopedia.org)
  • Baird, G. S., Zacharias, D. A. & Tsien, R. Y. Circular permutation and receptor insertion within green fluorescent proteins. (nature.com)
  • 3.0: hematopoietic cell-specific Lyn substrate 1 (HCLS1), Kelch domain-containing 6 (KLHDC6), sarcoglycan delta (SGCD), KIAA1706 protein (KIAA1706), RNA guanylyltransferase and 5′-phosphatase (RNGTT), chromosome 10 open reading frame 57 (C10orf57), chromosome 9 open reading frame 52 (C9orf52), and plasminogen activator, urokinase receptor (PLAUR). (molvis.org)
  • Binding of the signal to the CSS receptor is known to trigger a signaling cascade that results in the regulated proteolysis of the anti-sigma factor and the activation of the σECF in the cytosol. (vu.nl)
  • Zinc has "mimetic" activity where it is involved in a range of functions including insulin receptor signal transduction, insulin storage, secretion and tissues/organelle distribution, and inhibition of protein tyrosine phosphatases [ 5 - 8 ]. (hindawi.com)
  • E1/E2 heterodimer binding on CD81 activates the epithelial growth factor receptor (EGFR) signaling pathway (PubMed:22855500). (proteopedia.org)
  • Ferroptosis suppressor protein 1 (FSP1, also known as AIMF2, AMID or PRG3) is a recently identified glutathione-independent ferroptosis suppressor1-3, but its underlying structural mechanism remains unknown. (bvsalud.org)
  • Structural determinants of human proton-coupled folate transporter oligomerization: role of GXXXG motifs and identification of oligomeric interfaces at transmembrane domains 3 and 6, Biochem. (wayne.edu)
  • Their N-terminal hydrophilic domains may interact with cytoskeletal proteins and therefore play a cell structural role. (tcdb.org)
  • In her presentation, Dr. Goodeve identified three essential structural elements that control the development of the A2 domain. (esanum.com)
  • In principle, three structural elements were identified which control the A2 domain development. (esanum.com)
  • Binds RNA and may function as a RNA chaperone to induce the RNA structural rearrangements taking place during virus replication (PubMed:18033802). (proteopedia.org)
  • Recent structural work, in combination with functional studies, achieved new insight into how these proteins enable this synthetic feat 5,6 . (nature.com)
  • Bacterial siderophores are small molecules with unmatched capacity to scavenge iron from proteins and the extracellular milieu, where it mostly occurs as insoluble Fe 3+ . (biomedcentral.com)
  • In response to the presence of an iron carrier, the siderophore aerobactin, in the extracellular medium, IutY is processed by two different proteases, Prc and RseP, which results in the release and activation of the σIutY domain. (vu.nl)
  • DTX family members homodimerize and heterodimerize in vivo, suggesting that physical interactions between various DTX family members modify E3 activity and/or substrate availability. (nih.gov)
  • Although the C-terminal domain comprises a small percentage of the total protein, this domain in some cases (i) has PSD-95/Discs Large/ZO-1-binding motifs that may be important for protein-protein interactions (e.g. (tcdb.org)
  • To identify proteins interacting with alpha A-crystallin (CRYAA) and to investigate the potential role that these protein interactions play in the function of CRYAA using a human proteome (HuProt) microarray. (molvis.org)
  • A HuProt microarray composed of 17,225 human full-length proteins with N-terminal glutathione S-transferase (GST) tags was used to identify protein-protein interactions. (molvis.org)
  • The protein-protein interactions may help CRYAA carry out multifaceted functions. (molvis.org)
  • To understand the functions of CRYAA and how these are regulated, protein-protein interactions need to be investigated. (molvis.org)
  • Once biotinylated, (strept)avidin-biotin interactions can be utilized in a wide variety of applications of biochemistry and cell biology, including protein capture, immobilization, multimerizing, and bridging molecules. (watson-int.cn)
  • Docking domains enable non-covalent interactions between the polypeptides DEBS1-3. (nature.com)
  • I am committed to interdisciplinary research, and an example of the successes gained from this approach is the project with Prof A Nabok (Engineering Sheffield Hallam University) using total internal reflection ellipsometry to quantify protein-membrane interactions on native plant membranes and human cell lines. (brookes.ac.uk)
  • Program access grant to the STFC Harwell Laser Facility 'The Plant Cell Initiative: Protein interactions in the higher plant secretory pathway' 2017-2021 (approximate value of £200K). (brookes.ac.uk)
  • This group is composed of Mycobacterium tuberculosis putative mycofactocin radical SAM maturase MftC and similar proteins. (nih.gov)
  • In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. (rndsystems.com)
  • The NcACBP recombinant protein was able to specifically bind acyl-CoA esters in vitro . (biomedcentral.com)
  • More recently, I started to turn a portion of my research interest toward discovery and development of new folate-based therapeutics for cancer and other diseases that selectively use high affinity folate receptors and/or hPCFT without substrate activity for hRFC. (wayne.edu)
  • Herein, we report that BBAP and the human family of DTX proteins (DTX1, DTX2, and DTX3) function as E3 ligases based on their capacity for self-ubiquitination. (nih.gov)
  • The soluble fatty acid desaturases, such as the plant Acyl-carrier-protein (ACP) desaturase family, use acyl carrier protein thioesters as substrates, and use ferredoxin oxidoreductase and ferredoxin as electron donors. (springeropen.com)
  • Characterized protein members of the AE family are found in animals, plants and yeast. (tcdb.org)
  • In humans, the AE family (also called the SLC4 family) is composed of 10 paralogous members, among which are the proteins that perform Na + -independent Cl-HCO 3 - exchange (e.g. (tcdb.org)
  • SLC4 family members differ in their substrate dependence, charge transport stoichiometry, and tissue expression. (tcdb.org)
  • N-terminal domain, C-terminal domain, FGGY family of carbohydrate kinases [InterProScan]. (ntu.edu.sg)
  • transmembrane domain, AhpC/TSA family [InterProScan]. (ntu.edu.sg)
  • SHBG has a high binding affinity to dihydrotestosterone (DHT), medium affinity to testosterone and estradiol, and only a low affinity to estrone, DHEA, androstendione, and estriol. (cdc.gov)
  • Albumin, which exists in far higher concentrations than SHBG, also binds sexual steroids - although with a clearly lower binding affinity (e.g. about 100 times lower for testosterone). (cdc.gov)
  • CRYAA interacted with these proteins to maintain their solubility and decrease the accumulation of denatured target proteins. (molvis.org)
  • Conversely, it is thought to protect against several neurodegenerative diseases that are caused by the accumulation of misfolded proteins (11,12). (novusbio.com)
  • Here I pursued my scientific interests in subcellular protein localisation, bioinformatics, and mathematical modelling. (brookes.ac.uk)
  • Functional annotation clustering showed that they belong to cell cycle, organelle or nuclear lumen, protein transport, and DNA binding and repair clusters. (molvis.org)
  • Measured by its binding ability in a functional ELISA. (rndsystems.com)
  • In our present view, these areas are found for systems where several types of macromolecules (proteins, polysaccharides or polyelectrolytes) and/or amphiphiles interact to form a functional or destructive unit. (lu.se)
  • As this feature appears to be conserved in all other E1 and E1-like activating domains, the observed covalent E1-UBL self-conjugation reaction likely represents the evolutionary ancestor of most UBL pathways. (fnp.org.pl)
  • Measured by its ability to cleave a fluorogenic peptide substrate, Mca-YVADAPK(Dnp)-OH (Catalog # ES007 ). (rndsystems.com)
  • Biotinylated Recombinant Human ACE‑2 Fc Chimera Avi-tag (CHO Expressed) (Catalog # AVI10544) is measured by its ability to cleave a fluorogenic peptide substrate, Mca-YVADAPK(Dnp)-OH ( ES007 ). (rndsystems.com)
  • These opportunities are surveyed here against a background of the immunobiology of allergic sensitization and current state-of-the-art approaches to measurement of peptide/protein reactivity. (cdc.gov)
  • QLY-related compounds berberine, dioscin and sophocarpine showed high binding affinities to SIRT1, stabilized this protein, and inhibited its deacetylation activity in vitro. (bvsalud.org)
  • During its interaction with vWF, ADAMTS13 undergoes a conformational change, which increases its activity with the substrate. (esanum.com)
  • A yeast complementation assay showed that heterologous expression of NcACBP rescued the phenotypic defects in Δacbp yeast, indicating of the binding activity of NcACBP in vivo . (biomedcentral.com)
  • The cholesterol transfer activity of SCARB1 allows E2 exposure and binding of E2 to SCARB1 and the tetraspanin CD81 (PubMed:22767607, PubMed:12913001). (proteopedia.org)
  • GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. (lbl.gov)
  • Depletion of isoleucyl-tRNA results in inhibition of protein synthesis. (wikipedia.org)
  • The combined inhibition of protein synthesis and RNA synthesis results in bacteriostasis. (wikipedia.org)
  • protein_coding" "AGT23894","N559_2182","Klebsiella pneumoniae","putative ABC-type taurine transport system periplasmic binding component [Ensembl]. (ntu.edu.sg)
  • protein_coding" "AGT24100","N559_2404","Klebsiella pneumoniae","putative ABC transporter substrate binding protein [Ensembl]. (ntu.edu.sg)
  • protein_coding" "AGT25156","N559_3508","Klebsiella pneumoniae","putative short-chain dehydrogenase/reductase SDR [Ensembl]. (ntu.edu.sg)
  • We extracted whole-cell protein lysates from RF Borrelia cultures and synthesized six recombinant RF antigens (Borrelia immunogenic protein A (BipA) derived from four species of RF Borrelia, glycerophosphodiester phosphodiesterase (GlpQ), and Borrelia miyamotoi membrane antigen A (BmaA)) to detect reactivity in laboratory derived (Peromyscus sp. (cdc.gov)
  • The structures reveal a FAD-binding domain and a NAD(P)H-binding domain, both of which are shared with AIF and NADH oxidoreductases4-9, and a characteristic carboxy-terminal domain as well. (bvsalud.org)
  • An acyl-[acyl-carrier protein] + NAD(+) = a trans-2,3-dehydroacyl-[acyl- carrier protein] + NADH. (cathdb.info)
  • However, some researches revealed that the cytochrome b5 domain play a key role in the synthesis of unsaturated fatty acids as an electron donor to activate desaturase (Pereira et al. (springeropen.com)
  • protein_coding" "AGT24181","N559_2495","Klebsiella pneumoniae","cytochrome C biogenesis protein transmembrane region [Ensembl]. (ntu.edu.sg)
  • The recombinant NcACBP protein was expressed to verify the binding activities of NcACBP in vitro , and the heterologous expression of NcACBP in Δacbp yeast in vivo . (biomedcentral.com)
  • 10. Matherly, L.H. and Hou, Z.: Structure and function of the reduced folate carrier: a paradigm of a major facilitator superfamily mammalian nutrient transporter. (wayne.edu)
  • These are the ATP-Binding Cassette (ABC) superfamily and the Major Facilitator Superfamily (MFS). (embl.de)
  • Thus, the proteins that transport zinc likely facilitate cell signaling processes that contribute to glycemic control in peripheral tissues by modulating cytosolic zinc concentrations. (hindawi.com)
  • Sex hormone-binding globulin (SHBG) is the blood transport protein for testosterone and estradiol. (cdc.gov)
  • We performed a random mutagenesis screen of the unique IutY protein of Pseudomonas putida, a protein that combines a cytosolic σECF domain and a periplasmic anti-sigma factor domain in a single polypeptide. (vu.nl)
  • In contrast, Prc is only required for mutant variants with a periplasmic domain longer than 50 amino acids, which indicates that the periplasmic region of IutY is trimmed down to ~50 amino acids creating the RseP substrate. (vu.nl)