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CatecholsCatechol 2,3-DioxygenaseDioxygenasesCatechol 1,2-DioxygenaseSubstrate SpecificityOxygenasesEstrogens, Catechol4-Hydroxyphenylpyruvate DioxygenaseProtocatechuate-3,4-DioxygenaseMolecular Sequence DataCysteine DioxygenaseMannitol PhosphatesAmino Acid SequenceKineticsTryptophan OxygenaseBinding SitesRNA, CatalyticCatalysisCatechol O-MethyltransferaseHydrolysisEndopeptidasesHomogentisate 1,2-DioxygenasePseudomonas3-Hydroxyanthranilate 3,4-DioxygenaseCaspasesBase SequenceBiodegradation, EnvironmentalKynurenineModels, MolecularRecombinant ProteinsEscherichia coliProtease InhibitorsPeptide HydrolasesProtein BindingSerine EndopeptidasesHydrogen-Ion ConcentrationPseudomonas putidaCysteine EndopeptidasesHydroxybenzoatesMutagenesis, Site-DirectedRhodococcusPeptidesPhenolChlorobenzenesTryptophanMixed Function OxygenasesCatechol OxidaseSorbic AcidChlorobenzoatesBenzoatesTolueneOxidation-ReductionApoptosisSphingomonasCloning, MolecularDNA CleavageBurkholderiaSequence Homology, Amino AcidBenzoic AcidGenes, BacterialMutationBacterial ProteinsCatalytic DomainAlcaligenesGentisatesAdipatesCrystallography, X-RayBenzeneChromatography, High Pressure LiquidHydroxylationComamonasEquileninHomogentisic AcidPlasmidsProtein ConformationStructure-Activity RelationshipAcinetobacterMolecular WeightPhenolsProtein Structure, TertiaryIronHydrocarbons, AromaticCleavage Stage, OvumPyrogallolElectrophoresis, Polyacrylamide GelBrevibacteriumHydroquinonesSequence AlignmentModels, ChemicalRalstoniaStereoisomerismParabensRNA CleavageKetoglutaric AcidsArthrobacterOxidoreductasesPlanococcus BacteriaSpectrophotometryCell LineEnzyme Activation

SpecificityEnzymesPathwayProtocatechuateExtradiol dioxygenaseStructurallyDegradationEnzymeOrthoCharacterizationDienelactoneGeneResiduesGlyceraldehyde-3-phosphate dPathwaysInhibitorsPseudomonasRegulationReactionDiagnosticDerivativesActive siteHydrataseTemperatureGenesMetaProteinSitesToxicLargeAcidGroupShowFormsWaterRoleCommon

Specificity5

• However, studies could show that a R133H substitution switches the specificity of 3,4-PCD towards catechol. (uni-ulm.de)
• Analysis of 3,4-PCD activity toward catechol and PCA revealed further substitutions with changed substrate specificity. (uni-ulm.de)
• A 3-D homology model of BphC_JF8 provided a basis for understanding the substrate specificity, quaternary structure and stability of the enzyme. (muni.cz)
• Substrate specificity of catechol oxidase from Lycopus europaeus and characterization of the bioproducts of enzymic caffeic acid oxidation. (lookformedical.com)
• The substrate specificity of catechol oxidase from Lycopus europaeus towards phenols is examined. (lookformedical.com)

Enzymes6

• Catechol dioxygenases are metalloprotein enzymes that carry out the oxidative cleavage of catechols. (wikipedia.org)
• This class of enzymes incorporate dioxygen into the substrate. (wikipedia.org)
• Both enzymes are specialized for their substrate. (uni-ulm.de)
• The metal ligands and active site residues of extradiol dioxygenases are conserved although several amino acid residues found exclusively in enzymes which preferentially cleave bicyclic substrates are missing in BphC_JF8. (muni.cz)
• Oxygenases belong to the oxidoreductive group of enzymes (E.C. Class 1), which oxidize the substrates by transferring oxygen from molecular oxygen (O 2 ) and utilize FAD/NADH/NADPH as the co-substrate. (biomedcentral.com)
• Peripheral enzymes, particularly oxygenases and dehydrogenases, were found to transform structurally diverse substrates into one of these central intermediates by bringing about the hydroxylation of the aromatic nucleus (Fig. 2A ), and hence it is thought that bacteria have developed these enzymes to extend their substrate range [ 5 ]. (biomedcentral.com)

Pathway6

• Dienelactone hydrolases play a crucial role in chlorocatechol degradation via the modified ortho cleavage pathway. (inrae.fr)
• In this divergent mode, a metal-dependent dioxygenase channels these dihydroxylated intermediates into one of the two possible pathways: the meta -cleavage pathway or the ortho -cleavage pathway [ 7 - 9 ] (Fig. 1 ). (biomedcentral.com)
• 6 identified a gene cluster, hbpCAD , encoding the upper metabolic pathway of OPP which involves the transformation of OPP to 2-hydroxypenta-2,4-dienoateand benzoic acid (BA). (nature.com)
• The downstream transformation of BA involved a meta -cleavage pathway, although its genetic organization and function was not revealed and the overall network of genes driving the full metabolic pathway of OPP is still not known. (nature.com)
• 2021. Differential roles of three different upper pathway meta ring cleavage product hydrolases in the degradation of dibenzo-p-dioxin and dibenzofuran by Sphingomonas wittichii strain RW1. (nih.gov)
• 2021. Separate upper pathway ring cleavage dioxygenases are required for growth of Sphingomonas wittichii strain RW1 on dibenzofuran and dibenzo-p-dioxin. (nih.gov)

Protocatechuate3

• Catechol dioxygenases belong to the class of oxidoreductases and have several different substrate specificities, including catechol 1,2-dioxygenase (EC 1.13.11.1), catechol 2,3-dioxygenase (EC 1.13.11.2), and protocatechuate 3,4-dioxygenase (EC 1.13.11.3). (wikipedia.org)
• The enzyme protocatechuate 3,4-dioxygenase (3,4-PCD) catalyzes the ring cleavage of protocatechuate (PCA) in Acinetobacter baylyi. (uni-ulm.de)
• In the convergent mode, structurally diverse aromatic compounds are converted to one of a few aromatic ring cleavage substrates such as catechol, gent sate, protocatechuate and their derivatives [ 5 ]. (biomedcentral.com)

Extradiol dioxygenase1

• It exhibited a half life of 30 min at 80 C and 81 min at 75 C, making it the most thermostable extradiol dioxygenase studied. (muni.cz)

Structurally1

• Catechol 1,2-dioxygenase is structurally similar to 3,4-PCD. (uni-ulm.de)

Degradation1

• A number of bacteria that can degrade a variety of aromatic compounds have been identified and the pathways involved in the degradation have been extensively characterized [ 3 , 4 ]. (biomedcentral.com)

Enzyme4

• The enzyme is resistant to denaturation by various chelators and inhibitors (EDTA, 1,10-phenanthroline, H2O2, 3-chlorocatechol) and did not exhibit substrate inhibition even at 3 mM 2,3-dihydroxybiphenyl. (muni.cz)
• As the thiolate anion is not energetically favoured in the hydrophobic interior of the enzyme, it swings into the active site where it can be stabilized by the His202 imidazolium and the dipole of helix C. The Cys123 thiolate which now lies adjacent to the acyl carbon of the substrate, is thus generated only in the presence of substrate. (inrae.fr)
• Specific activities of Catechol-2,3-dioxygenase and effects of temperature and pH and their stabilities on the enzyme relative activities were observed. (openmicrobiologyjournal.com)
• An enzyme of the oxidoreductase class that catalyzes the reaction between catechol and oxygen to yield benzoquinone and water. (lookformedical.com)

Ortho1

• Genomic analysis identified two orthologous operons encoding the ortho -cleavage of benzoic acid (BA) ( ben/cat ). (nature.com)

Characterization1

• Cloning and characterization of a novel thermostable Mn(II)-dependent 2,3-dihydroxybiphenyl 1,2-dioxygenase from a PCB and naphthalene degrading Bacillus sp. (muni.cz)

Dienelactone1

• Our inhibitor binding studies suggest that its substrate, dienelactone, is held in the active site by hydrophobic interactions around the lactone ring and by the ion pairs between its carboxylate and Arg-81 and Arg-206. (inrae.fr)

Gene3

• The Pseudomonas putida xylE gene, which encodes catechol 2,3-dioxygenase, is often used as a reporter to quantitate gene expression. (wikipedia.org)
• For all significantly differentially expressed microRNAs inside of the 2 groups, we now have produced gene sets from their expressed target genes. (pdpksignaling.com)
• Catechol 2,3-dioxygenase (C23O) gene was found andamplified with the designed primers from the total DNA of C-14-1. (researchgate.net)

Residues1

• The results of cDNA cloning, peptide mapping, and amino acid sequencing of PPAE revealed that PPAE is synthesized as prepro-PPAE with 441 amino acid residues and is activated from pro-PPAE by cleavage of a peptide bond between Lys152 and Ile153. (lookformedical.com)

Glyceraldehyde-3-phosphate d1

• Glyceraldehyde 3-phosphate dehydrogenase [Interproscan]. (ntu.edu.sg)

Pathways1

• In A. baylyi this mechanism was investigated using the degradative pathways for the aromatic substrates and dicarboxylates. (uni-ulm.de)

Inhibitors1

• Substrates containing a -COOH group are inhibitors for catechol oxidase. (lookformedical.com)

Pseudomonas2

• They isolated a Pseudomonas azelaica strain HPB1 which was able to degrade OPP through the production of 2,3-dihydroxybiphenyl. (nature.com)
• Metabolism of 2,4,6-trinitrotoluene by Pseudomonas sp. (paperbase.net)

Regulation1

• frequently the pre-B causes with the Toxicity, a expected other Convention in the Notch aurora has which is an cis-Golgi on the S2 antigen regulation fibrin many to the switching cleavage. (evakoch.com)

Reaction1

• An example of the reaction carried out by catechol 1,2-dioxygenase is the formation of cis,cis-muconic acid from catechol, shown below. (wikipedia.org)

Diagnostic1

• We investigated the diagnostic accuracy of the 2 multiplex protein array platforms for detecting a bladder-cancer-associated d iagnostic signature in samples from a cohort of 80 topics (40 with bladder most cancers). (kits-elisa.com)

Derivatives1

• B, solute carrier relatives six member 2, solute carrier relatives 18 member one, and transcription factors and homeobox genes involved in neural crest derived cell de velopment, paired like homeobox 2a and 2b, GATA binding protein two and 3, heart and neural crest derivatives expressed two. (pdpksignaling.com)

Active site1

• The active site of catechol dioxygenases most frequently contains iron, but manganese-containing forms are also known. (wikipedia.org)

Hydratase1

• Cytochrome P450 monooxygenase (EstP1), 2-keto-4-pentenoate hydratase, pyruvate dehydrogenase, acetyl-CoA acetyltransferase, TonB-dependent receptor were involved in E2 catabolism. (bvsalud.org)

Temperature1

• A decrease in Km accompanied an increase in temperature and the Km value of 0.095 uM for 2,3-dihydroxybiphenyl (at 60 C) is among the lowest reported. (muni.cz)

Genes2

• The second ben/cat operon was located in a 92-kb scaffold along with (i) an operon ( opp ) comprising genes for the transformation of OPP to BA and 2-hydroxypenta-2,4-dienoate (and genes for its transformation) and (ii) an incomplete biphenyl catabolic operon ( bph ). (nature.com)
• Genes & Development 21 (3): 316-331. (mdc-berlin.de)

Meta2

• A novel thermostable Mn(II)-dependent 2,3-dihydroxybiphenyl 1,2-dioxygenase (BphC_JF8) catalyzing the meta-cleavage of the hydroxylated biphenyl ring was purified from the thermophilic biphenyl and naphthalene degrader, Bacillus sp. (muni.cz)
• 4-OH-E1 meta-cleavage was catalyzed by a dioxygenase encoded by IM701_20340 or IM701_21000 or IM701_09625. (bvsalud.org)

Protein2

• Two compounds, Compound 1 (1,4-dihydrophenanthrolin-4-one-3-carboxylic acid) and Compound 5 [8-(N-butyl-N-ethylcarbamoyl)-1,4-dihydrophenathrolin-4-one-3-carboxylic acid], with comparable potencies in vivo, were chosen to investigate the effect of the inhibition of the hydroxylation of newly synthesized uterine collagen on the turnover of this protein in vivo. (embl.de)
• Serum insulin-like growth factor (IGF)-I and IGF-binding protein-3 concentrations and prostate cancer risk: results from the European Prospective Investigation into Cancer and Nutrition. (mdc-berlin.de)

Sites1

• Therefore, engineering of Catechol 2,3-dioxygenase may be employed for application on bioremediation of polluted sites. (openmicrobiologyjournal.com)

Toxic1

• Many of these compounds have been reported to be toxic to the living organisms [ 3 ]. (biomedcentral.com)

Large1

• Its application results in the production of large wastewater volumes which require treatment on site 2 . (nature.com)

Acid1

• 2-keto-4-pentenoic acid might be an emblematic product of such process. (bvsalud.org)

Group2

• Unlike the proteases, DLH seems to protonate the incipient leaving group only after the collapse of the first tetrahedral intermediate, rendering DLH incapable of hydrolyzing amide analogues of its ester substrate. (inrae.fr)
• A group of 1,2-benzenediols that contain the general formula R-C6H5O2. (lookformedical.com)

Show1

• The results show that under pH 3-6, the adsorption of U(VI) by YF1 live cells increased with the increase of the pH. (bvsalud.org)

Forms1

• When substrate binds, Arg206 forms a second ion pair with the anionic substrate and the Arg206/Glu36 ion pair weakens. (inrae.fr)

Water1

• They play a key role in the metabolism of organic compounds by increasing their reactivity or water solubility or bringing about cleavage of the aromatic ring. (biomedcentral.com)

Role1

• In this investigation, random PCR mutagenesis has been carried out to explore the functional role of the R133 in 3,4-PCD. (uni-ulm.de)

Common1

• The 2,3-dihydrobenzofuran derivative, 7-acetyl-4,6-dimethoxy-2-isopropenyl-2,3-dihydrobenzofuran, had an ED 50 value of 1.3 μg (5.4×10 -9 mol)/cm 2 against the common cutworm. (go.jp)