• Paratuberculosis is a chronic intestinal disease of animals caused by Mycobacte-rium avium subsp. (scielo.cl)
  • M. avium complex includes the species M. avium and M. intracellulare , with M. avium consisting of M. avium subsp. (cdc.gov)
  • Sera from interleukin-10-deficient (IL-10(-/-)) mice with spontaneous colitis displayed significantly higher M. avium subsp. (giostarindia.com)
  • Results from SNPs typing of gyrB and ITS, as well as from MIRU-VNTR analysis suggested the isolation of a single strain of M. avium subsp. (frontiersin.org)
  • None of the herd management practices studied were found to be associated with whether any animals in the herd would be positive for antibodies to M avium subsp paratuberculosis . (avma.org)
  • Conclusions and Clinical Relevance -Results suggest that the prevalence of antibodies to M avium subsp paratuberculosis among beef cows in the United States is low. (avma.org)
  • In the samples from nine animals (9/16), DNA from M. avium subsp. (johnes.org)
  • paratuberculosis (MAP) is the causative agent of Johne's disease, a chronic debilitating disease in ruminants. (biomedcentral.com)
  • paratuberculosis (MAP) is a causative agent of paratuberculosis (PTB), more commonly known as Johne's disease (JD), which is a chronic and debilitating disease in ruminants. (biomedcentral.com)
  • Mariana Assunção de Souza and 6 other Brazilian colleagues reported on the occurrence of Johne's disease (paratuberculosis) in cattle that were necropsied based on a positive comparative cervical skin test for bovine tuberculosis (bTB). (johnes.org)
  • paratuberculosis, the causative agent of Johne's Disease, by complement fixation. (tamu.edu)
  • paratuberculosis Infection. (pasteur.fr)
  • Paratuberculosis is a staged infection in which young calves acquire the infection in the first months of life, may progress into a prolonged asymptomatic stage of about 2-5 years and may eventually become clinically infected animals. (dtu.dk)
  • paratuberculosis (MAP) does not produce glycopeptidolipids (GPL) on the surface of the cell wall but a lipopentapeptide called L5P (also termed Lipopeptide-I or Para-LP-01) characterized in C-type (bovine) strains. (pasteur.fr)
  • These findings, together with bioinformatics tools available, could be extremely useful to design a better strategy for subclinical bovine paratuberculosis diagnosis. (degruyter.com)
  • paratuberculosis to enter bovine epithelial cells is influenced by preexposure to a hyperosmolar environment and intracellular passage in bovine mammary epithelial cells. (oregonstate.edu)
  • The aim of this work was to study the intercurrence of paratuberculosis in tuberculosis reactive cattle by the comparative cervical test. (johnes.org)
  • Paratuberculosis in cattle is clinically characterized by weight loss, emaciation and diarrhea and subclinically by reduced milk production leading to considerable economic losses to farming community. (dtu.dk)
  • paratuberculosis (MAP), which has some pathological features similar to Crohn's disease (CD) in humans. (scielo.cl)
  • Detection of M. paratuberculosis by IS900 PCR was consistent (24/24 PCR assays) when about 100 cfu/ml were present, whereas detection was variable (12/24 PCR assays) at concentrations as low as 10 cfu/ml. (psu.edu)
  • paratuberculosis in raw milk. (psu.edu)
  • The IS900 PCR assay was evaluated by inoculating raw bulk milk and Middlebrook's 7H9 broth with 0 to 10 8 cfu/ml of each of four American Type Culture Collection strains of M. paratuberculosis. (psu.edu)
  • Under experimental conditions, both milk culture on Herrold's egg yolk medium slants, and IS900 PCR could detect 10 to 100 cfu/ml of M. paratuberculosis. (psu.edu)
  • Out of 211 animals examined, nine (4%) and 69 (33%) were positive for M. paratuberculosis by milk culture and IS900 PCR from milk, respectively. (psu.edu)
  • A total of 20 bulk tank milk sample aliquots (one sample, four aliquots from each herd) were also examined, of which 10 (50%) were positive for M. paratuberculosis by IS900 PCR. (psu.edu)
  • The IS900 PCR amplified product of 229-bp obtained on testing of quarter milk and bulk tank milk samples was confirmed to be the IS900 of M. paratuberculosis by DNA sequence analysis. (psu.edu)
  • The results of this study suggest that M. paratuberculosis can be detected directly from quarter milk and bulk tank milk by IS900 PCR. (psu.edu)
  • paratuberculosis (MAP) in milk and mammary apparatus of seropositive ewes, samples of intestine, mammary gland, mammary and mesenteric lymph nodes, and milk were collected from 20 Comisana breed sheep for bacterial culture and PCR (IS900). (unicam.it)
  • NBRC 14428 BCJ48598.1 ncbi GH76 Bacteria Actinopolymorpha singaporensis DSM 22024 SDS32957.1 ncbi GH76 Bacteria Actinopolymorpha singaporensis DSM 22024 SDS42219.1 ncbi GH76 Bacteria Actinopolymorpha singaporensis DSM 22024 SDS63373.1 ncbi GH76 Bacteria Actinopolymorpha singaporensis DSM 22024 SDR86201.1 ncbi GH76 Bacteria Actinosynnema mirum ATCC 31280 QUF02050.1 ncbi GH76 Bacteria Actinosynnema mirum DSM 43827 ACU40727.1 ncbi GH76 Bacteria Actinosynnema pretiosum subsp. (cazy.org)
  • vulgaris 3BBH6 BBK99968.1 ncbi GH76 Bacteria Alistipes onderdonkii subsp. (cazy.org)
  • paratuberculosis in dairy herds, Am. J. Vet. (vetres.org)
  • The concentration of M. paratuberculosis was determined by using quantitative, real-time PCR to target the IS900 sequence in DNA extracts. (usda.gov)
  • paratuberculosis -macrophage interactions, Clin. (vetres.org)
  • The resulting sequence was compared with those stored in the International Nucleotide Sequence Database (8) , showing the signature sequence of M. avium / M. paratuberculosis , which is identical for both species and confirmed the AccuProbe results. (cdc.gov)
  • paratuberculosis colonies growing on mycobactin-supplemented Middlebrook agar. (cdc.gov)
  • paratuberculosis (MAP), the causative agent of Johne disease in ruminants, has been incriminated as the cause of Crohn disease in humans. (cdc.gov)
  • paratuberculosis: pathogen, pathogenesis and diagnosis. (degruyter.com)
  • paratuberculosis (MAP) shown a clear association of MAP with the presence of type 1 diabetes mellitus (T1DM). (biomedcentral.com)
  • High concentrations of M. paratuberculosis were detected within the biofilms after three days (around 1 X 105 cells per cm2). (usda.gov)
  • The goal of this 365 day study was to evaluate the ability of M. paratuberculosis to persist in mixed-community biofilms on coupons composed of common livestock watering trough materials. (usda.gov)
  • paratuberculosis in infected tissues by new species specific immunohistological procedures, Clin. (vetres.org)
  • paratuberculosis-specific genes impairs in vivo fitness. (bvsalud.org)
  • It is often abbreviated M. paratuberculosis or M. avium ssp. (wikipedia.org)
  • In the case we describe, mycobacterial growth could be detected in liquid media in 8 of 21 specimens, all confirmed as M. avium complex/ M. paratuberculosis . (medscape.com)
  • M. avium complex includes the species M. avium and M. intracellulare , with M. avium consisting of M. avium subsp. (cdc.gov)
  • The resulting sequence was compared with those stored in the International Nucleotide Sequence Database (8) , showing the signature sequence of M. avium / M. paratuberculosis , which is identical for both species and confirmed the AccuProbe results. (cdc.gov)
  • It belongs to the same family of insertion sequences as IS901, IS902, and IS1110, described in M. avium subsp. (biomedcentral.com)
  • The acquisition of foreign genes via horizontal gene transfer has been postulated to contribute to its pathogenesis, as these genetic elements are absent from its putative ancestor, M. avium subsp. (psu.edu)
  • paratuberculosis field isolates, 1 American Type Culture Collection (ATCC) M. avium subsp. (tamu.edu)
  • paratuberculosis isolate (ATCC 19698), and 2 M. avium subsp. (tamu.edu)
  • AFLP analysis revealed a high degree of genomic polymorphism among M. avium subsp. (tamu.edu)
  • paratuberculosis isolates that may be used to establish diagnostic patterns useful for the epidemiological tracking of M. avium subsp. (tamu.edu)
  • Primers were generated internal to these regions for use in PCR analysis and applied to the M. avium subsp. (tamu.edu)
  • An appropriate PCR product was obtained in 79 of 80 reactions, while the M. avium subsp. (tamu.edu)
  • This work revealed the presence of extensive polymorphisms in the genomes of M. avium subsp. (tamu.edu)
  • paratuberculosis-specific sequences studied, one revealed a 5,145-bp region with no homologue in the M. avium subsp. (tamu.edu)
  • UV treatment proved to be less effective in killing M. avium subsp. (calfstart.com)
  • The FASTPlaqueTB phage assay provided more rapid enumeration of surviving M. avium subsp. (calfstart.com)
  • Despite the fact that plaque counts were consistently 1 to 2 log10 lower than colony counts throughout the study, UV inactivation rates for M. avium subsp. (calfstart.com)
  • Deer experimentally inoculated with either M. avium subsp. (nih.gov)
  • avium has been found to cause tuberculosis in captive and free-living birds, while M. avium subsp. (thepigsite.com)
  • The aim of this study was to examine isolates of M. avium subsp. (thepigsite.com)
  • In order to determine the best screening method, a panel of 14 isolates of M. avium subsp. (thepigsite.com)
  • Virulence and immunogenicity of genetically defined human and porcine isolates of M. avium subsp. (sciensano.be)
  • Plasma samples were tested for antibody against M paratuberculosis , and IFN-γ after stimulation with purified protein derivative tuberculin from M bovis or M avium . (avma.org)
  • Paratuberculosis infection of nonruminant wildlife in Scotland. (nih.gov)
  • paratuberculosis (MAP) is an obligate intracellular pathogen that infects many ruminant species. (psu.edu)
  • paratuberculosis (MAP) is an intracellular pathogen causative of Jonhe disease or paratuberculosis in most domestic or wild rumiant species. (cibnor.mx)
  • paratuberculosis (MAP), the causative agent of Johne disease in ruminants, has been incriminated as the cause of Crohn disease in humans. (cdc.gov)
  • paratuberculosis specific antigens in DNA vaccinated and Map infected mice. (sciensano.be)
  • paratuberculosis (PPD)--and antigens: tetanus toxoid and KLH. (nih.gov)
  • paratuberculosis or M. bovis BCG Pasteur did not produce cross-reactive antibodies that could be detected by the DPP VetTB assay. (nih.gov)
  • paratuberculosis derived using the phage assay and culture results were not significantly different (P 0.077). (calfstart.com)
  • Conclusions and Clinical Relevance -No significant association was detected among cattle testing positive for M paratuberculosis as determined by microbial culture of feces and antibody ELISA and positive CFT test and IFN-γ assay results for M bovis . (avma.org)
  • Since paratuberculosis is often subclinical, under-reporting is suspected, even though the disease is notifiable in numerous countries. (nih.gov)
  • Herds were selected based on the presence of risk factors for paratuberculosis (74 herds), sampling was performed by veterinarians who collected negative samples in all of their herds in a previous survey (75 herds) and clinical paratuberculosis cases (nine herds). (vetline.de)
  • En base a los resultados de predicción inmunogénica, se seleccionaron los genes que codifican para las proteínas MAP0164, MAP0189 y MAP2100. (cibnor.mx)
  • Luego, los genes MAP2100 y MAP0189 se clonaron en el vector p320 para la modificación genética del cloroplasto de C. reinhardtii. (cibnor.mx)
  • paratuberculosis in Middlebrook 7H9 broth and whole and semiskim milk was investigated using a laboratory-scale UV machine that incorporated static mixers within UV-penetrable pipes. (calfstart.com)
  • paratuberculosis suspended in milk (0.5- to 1.0-log10 reduction per 1,000 mJ/ml) than that suspended in Middlebrook 7H9 broth (2.5- to 3.3-log10 reduction per 1,000 mJ/ml). (calfstart.com)
  • paratuberculosis (MAP), causes weight loss, diarrhoea, and reduced milk yields in clinically infected cattle. (aber.ac.uk)
  • paratuberculosis (within 24 h) than culture on Herrold's egg yolk medium (6 to 8 weeks). (calfstart.com)
  • Fecal samples were submitted for microbial culture for M paratuberculosis . (avma.org)
  • Paratuberculosis in domestic livestock entails significant economic losses due to several factors (e.g. reduced production, premature culling and increased veterinary costs). (nih.gov)