Centrifugation is a process that involves the use of a centrifuge to separate particles from a solution based on their size, shape, density, or other physical properties. (microbiologynote.com)
These samples would be pooled and processed by using available technology to isolate virus particles en masse, recover viral nucleic acids, produce amplified shotgun libraries, carry out shotgun sequencing of the mixture of viral genomes, and reconstruct these genomes in silico with the techniques originally developed to sequence the entire human genome from random fragments. (cdc.gov)
Cells3
Centrifugation is a widely used technique in research, industry, and clinical laboratories, and it has many applications, including the purification of biological samples, the separation of cells and organelles, the analysis of proteins and nucleic acids, and the preparation of diagnostic specimens. (microbiologynote.com)
Figure 10: Southern blot of vector DNA isolated from HEK-293 cells infected with rAAV containing genomes of sizes 4,675 nucleotides (nt), 5,302 nt and 6,019 nt in the presence of transfected adenovirus helper (XX680) and AAV2 helper (pHelper). (nature.com)
It is possible to isolate these antigen by extracting the intact cells in a hypertonic buffer solution at a pH of 6-8 and at a temperature below the denaturing point of the antigenic protein and fractionating the resultant antigen from the extract thereby obtained. (everypatent.com)
Relative2
Relative centrifugal force is a measurement for the rate of strength in rotors that are of different sizes and types. (microbiologynote.com)
Facilitated by the relative ease with which viruses can be isolated from seawater (using commercial filters), investigators in this area have examined a broad and essentially unbiased population of viral agents at the genome sequence level (including phage) and estimated the number of different genomes present (~5,000) ( 1 - 3 ). (cdc.gov)
Viruses2
JE viruses isolated from human cases in Japan in 1935 and in Beijing in 1949 provided the prototype Nakayama, Beijing and P3 strains respectively that are in principal use in vaccine production. (cdc.gov)
Facilitated by the relative ease with which viruses can be isolated from seawater (using commercial filters), investigators in this area have examined a broad and essentially unbiased population of viral agents at the genome sequence level (including phage) and estimated the number of different genomes present (~5,000) ( 1 - 3 ). (cdc.gov)
Sample1
Sample preparation for in vivo measurement for respiration rate and photosynthetic oxygen evolution. (elte.hu)
Title1
Type 508 Accommodation and the title of the report in the subject line of e-mail. (cdc.gov)