Study protein stability. Medical search. Frequent questions

For the study, the authors used this cDNA display proteolysis strategy to systematically come up with about 776,000 high-quality folding stability measurements for hundreds of naturally occurring or engineered protein domains containing single or double amino acid mutations under consistent conditions, for example, making it possible to consider everything from the folding stability contributions of specific amino acids to the interactions affected by protein domain folding stability-related selective pressures. (genomeweb.com)
Monitor the hydrodynamic properties of biomacromolecules and protein-protein interactions. (utmb.edu)
This information can be used to analyze protein-protein interactions, the presence of oligomeric states, and conformational rearrangement of molecules. (utmb.edu)
Protein drug physical stability and analytical methods to study interactions between protein drugs and formulation excipients. (otago.ac.nz)
Two recent papers address these questions, focusing on TDP-43's stability and protein-protein interactions. (alzforum.org)
Ling plans to examine TDP-43 stability and protein-protein interactions in neurons and patient-derived cells. (alzforum.org)
The intra- and intermolecular interactions that govern the dynamic nature and function of immunoglobulins may also influence their stability. (ku.edu)
Therefore, a better understanding of interactions that govern protein structure, dynamics and conformational stability at low and high concentrations and in presence of excipients should aid in designing, optimizing and developing rational formulation conditions for protein based therapeutics. (ku.edu)
Furthermore, it was determined that potential interactions and factors modulating solution viscosity at high protein concentrations may also result in changes in their extinction coefficients. (ku.edu)
The work presented in this dissertation provides evidence that factors modulating protein dynamics and those governing intra- and intermolecular interactions can influence the conformational stability, aggregation and viscosity of proteins in solution. (ku.edu)
Our understanding of the stability of emulsions may leap forward as UK scientists report the use of an atomic technique to probe the interactions of pectin. (foodnavigator.com)
One of the PD mouse models involved characterizing the protein interactions induced by mutated leuceine-rich repeat kinase 2 (LRRK2) at a pre-symptomatic time point (3 months old). (duke.edu)
Furthermore, these results establish a new biophysical link between the hit proteins identified and their role in aging, LRRK2 protein interactions, and PD progression. (duke.edu)
Stability, dynamics and interactions of proteins: packing and electrostatics. (lu.se)

The wavelength ranges used for the structural assessment of protein are broadly classified into three regions. (utmb.edu)
In a recent study using Molecular Dynamics simulations, a considerable correlation between thermal stability and structural rigidity of members of family 5 with solved structures has been proved. (wikipedia.org)
The profilaggrin gene is part of a cluster of genes on 1q21 that encodes for structural proteins expressed in terminally differentiating epidermis. (medscape.com)
Solution molecular biophysics and thermodynamic techniques are used both as a primary research tool as well as means to structural studies (for example in the exploration of conditions for crystallization of proteins). (utmb.edu)
Protein structural changes during freezing and subsequent thawing are of great importance to a variety of biopharmaceutical applications. (nist.gov)
In this work, we studied the influence of non-ionic surfactants (polysorbate 20 and poloxamer 188) on protein structural changes during freeze and thaw using lysozyme as a model protein. (nist.gov)
Urea-induced denaturation study shows that the SRLFDQFFG sequence contributes significantly to the structural stability. (nih.gov)
Fluorescence resonance energy transfer studies show that the rate of exchange of the subunits in the alpha Adel-crystallin oligomer is higher compared with that in the alpha A-crystallin oligomer, suggesting that this region contributes to the oligomer dynamics in addition to the higher order assembly and structural stability. (nih.gov)
The key arguments for carbon-based life are the ubiquity of organic (carbon-containing) compounds in the universe and the ability of carbon to form stable compounds with many elements, thus creating the wide variety of structural, catalytic, and informational macromolecules (very large molecules, such as DNA and proteins) that make up Earth life. (nationalacademies.org)
The structural complexity of these molecules requires careful handling to ensure integrity and stability of the drug. (lu.se)
Overall, the study highlights the essential need for atomic resolution site-specific structural information to understand and to predict the stability of mutant proteins. (rcsb.org)

This paper, written by Drs. John Wang and Musetta Hanson, is a review of concerns in formulating injectable products containing peptides and proteins. (pda.org)
Of these 52 peptides 22 were identified with early disease progression, 5 peptides showed late disease progression, 5 peptides reported a gradual difference in stability over disease progression and 20 peptides indicated no disease progression trend. (duke.edu)

The research reported in Nature , led by Manuela Pellegrini, Ph.D., and Arkady Celeste, Ph.D., of NCI's Experimental Immunology Branch, shows that addition of a mutant version of the ATM protein (Atm-S1987A) that is unable to autophosphorylate can restore normal function to mice lacking ATM. (nih.gov)
Mice with the Atm-S1987A mutant protein appear normal and do not have the defects that are observed in mice without ATM. (nih.gov)
The researchers attribute the rescue of ATM-deficient mice to the ability of the Atm-S1987A mutant protein to function normally without autophosphorylation. (nih.gov)
This mutant ATM protein also migrated to the site of DNA breaks and phosphorylated other proteins appropriately. (nih.gov)
Therefore, the mutant Atm-S1987A protein exhibits normal phosphorylation activity and is sufficient to trigger an efficient response to radiation. (nih.gov)
Glycerol density gradient centrifugation and dynamic light scattering studies show that the multimeric size of the mutant proteins is significantly reduced, indicating a role for this motif in higher order organization of the subunits. (nih.gov)
Ling and colleagues wondered if the mutant proteins hang around in the cell longer than the wild-type does. (alzforum.org)
New research by Koji Yamanaka and colleagues from the Laboratory for Motor Neuron Disease at the RIKEN Brain Science Institute has now revealed that increased stability of mutant TDP-43 is associated with earlier onset of ALS. (medicalxpress.com)
This increased stability also made the mutant TDP-43 molecules more toxic to the cells. (medicalxpress.com)
Accelerated disease onset with stabilized familial Amyotrophic Lateral Sclerosis (ALS)-linked mutant TDP-43 proteins. (medicalxpress.com)

This is the expression of all the proteins a mammal has in their genome. (studyfinds.org)
Professor Forsburg studies how chromosome duplication and maintenance contributes to overall genome stability using a simple model genetic system, the fission yeast Schizosaccharomyces pombe . (usc.edu)
Forsburg takes a holistic approach to studying how DNA replication stress contributes to genome stability. (usc.edu)

The current study looked at the difference in disease progression in patients with differing mutations. (genengnews.com)
Characteristic bands of aromatic residues (Trp, Tyr, and Phe) in the near-UV region can be used to examine the effect of mutations on the tertiary structure of proteins. (utmb.edu)
The goal of the present investigation is to examine the effects of missense mutations in the MERS-CoV S protein on protein stability and binding affinity with DPP4 to provide insight that is useful in developing vaccines to prevent coronavirus infection. (frontiersin.org)
We investigated the effects of MERS-CoV S protein viral mutations on protein stability and binding affinity. (frontiersin.org)
In addition, we studied all DPP4 mutations and found the functional substitution R336T weakens both DPP4 protein stability and S-DPP4 binding affinity. (frontiersin.org)
These findings provide further information on how mutations in coronavirus S proteins effect protein function. (frontiersin.org)
Several mutations in the N terminus of the G-protein-coupled receptor rhodopsin disrupt NXS/T consensus sequences for N-linked glycosylation (located at N2 and N15) and cause sector retinitis pigmentosa in which the inferior retina preferentially degenerates. (jneurosci.org)
Mutations affecting glycosylation of the heptahelical G-protein-coupled receptor rhodopsin are associated with retinitis pigmentosa (RP), a disease characterized by progressive degeneration of photoreceptors. (jneurosci.org)

N-linked glycosylation is the most prevalent posttranslational modification of plasma membrane and secretory proteins and participates in many important biological roles such as protein folding, intracellular targeting, immune response, cell adhesion, and protease resistance. (jneurosci.org)
The second study, posted online in the Journal of Biological Chemistry on June 16, suggests one possible mechanism by which TDP-43 might bind other proteins. (alzforum.org)
The new study reveals genes which take part in the biological processes that keep many mammal species alive. (studyfinds.org)
Proteins, such as immunoglobulins, are inherently dynamic molecules with unique biological functions. (ku.edu)
The biological variability of the protein stability measurements was low, and within the experimental error of the SPROX technique. (duke.edu)
However, four protein hits, dihydropyrimidinase-related protein 2, eukaryotic translation initiation factor 4A2, Rap1 GTP-GDP dissociation stimulator 1 and myelin basic protein, were identified with consistent thermodynamic stability in multiple mice within a biological state and as hits in multiple comparisons suggesting they are the most likely to be true positives. (duke.edu)
We have strategically improved the stability of the metal-lysozyme composites, specifically in biological fluids such as plasma and cytosol. (tus.ac.jp)
Our major finding suggests that mutual interaction of the two domains appears to be crucial for protein stability and its biological function. (europa.eu)
These beneficial biological properties have been extensively studied in humans and animal models, both in vitro and in vivo . (hindawi.com)
The course aims to provide a deeper understanding of some of the most important experimental methods used to determine the three-dimensional structures of proteins, as a basis for understanding their biological functions. (lu.se)
Protein phosphorylation is the most common post-translation modification of proteins and regulates many biological processes. (lu.se)

Rossetti, G. Impact of Cholesterol on the Stability of Monomeric and Dimeric Forms of the Translocator Protein TSPO: A Molecular Simulation Study. (preprints.org)
Here, we present a coarse-grained molecular dynamics study on the mouse protein in the presence of a physiological content and of an excess of cholesterol. (preprints.org)
The study appears in the journal Molecular Biology and Evolution . (studyfinds.org)
A molecular understanding of interrelationship between dynamics, function and conformational stability of immunoglobulins, both in solution and in presence of co-solutes, can be important for their pharmaceutical development. (ku.edu)
Special attention has been paid to conformational changes of protein matrix (inter domain movement of 12/15-lipoxygenase) in solution and molecular dynamics of oxygen as second lipoxygenase substrate. (europa.eu)
Molecular dynamics studied by NMR relaxation experiments. (lu.se)
Thus, in this study, the structure of the Keif peptide part have been investigated by using both atomisic molecular dynamics. (lu.se)

UTMB also pioneered the use of osmolytes in the stabilization of intrinsically unstructured regions of proteins ( 2 ) and the SBL has a long tradition of the use of protecting osmolytes in biophysical studies ( 3 ). (utmb.edu)

A variety of experimental methods sensitive to structure, dynamics and conformational stability of proteins in solution were employed in these studies. (ku.edu)
Different monoclonal antibodies within IgG1 isotype were used to investigate interrelationships between protein dynamics and conformational stability in absence and presence of co-solutes. (ku.edu)
These studies provide evidence that immunoglobulins belonging to the same IgG1 subtype can have notable differences in their conformational stability, dynamics, aggregation propensity, hydration properties and their response to co-solutes. (ku.edu)
In addition, alterations in protein dynamics (at a global protein level and in local regions with differences in solvent exposures) by stabilizing or destabilizing excipients were found to modulate the conformational stability of a monoclonal antibody. (ku.edu)

The outbreak strains showed identical nucleotide sequences in genes encoding three rotavirus proteins, VP7, VP8, and NSP4, but they were distinct from G9P[8] strains isolated in previous years. (cdc.gov)
In a Novartis-sponsored study in the New England Journal of Medicine, researchers found that a CRISPR-Cas9-based treatment targeting promoters of genes encoding fetal hemoglobin could reduce disease symptoms. (genomeweb.com)
Now, a new study has discovered a collection of genes which may explain what actually determines how long we live. (studyfinds.org)
Until now, the researchers say most studies have looked specifically at human genes. (studyfinds.org)
They then introduced the mutated genes into neurons growing in culture dishes in order to induce the cells to synthesize the mutated proteins. (medicalxpress.com)

Circular dichroism (CD) is a powerful spectroscopic technique used to study the changes in the structure and conformation of a protein. (utmb.edu)
Circular dichroism spectroscopic studies show changes in tertiary and secondary structure on deletion of the sequence. (nih.gov)
Through detailed crystallographic and spectroscopic analysis, the research team, which also comprised Assistant Professor Kenichi Kitanishi from TUS, Dr. Arshak Tsaturyan from Southern Federal University, and Professor Masaki Unno from Ibaraki University, among others, confirmed the formation of the hybrid protein CuST@lysozyme, and elucidated its structure. (tus.ac.jp)

The outer capsid proteins, VP4 and VP7, elicit neutralizing antibody immune responses that are both serotype-specific and cross-reactive ( 13 ). (cdc.gov)
SIV proteins, especially the viral core proteins (i.e., p24, capsid protein), are antigenically related to HIV-I proteins (9). (cdc.gov)
Some SIV isolates, however, are antigenically more related to HIV-2 than to HIV-I by cross-reactivity of viral capsid and envelope proteins. (cdc.gov)

The near-UV CD (250-320 nm) monitors changes in the vicinity of aromatic amino acids in a protein, investigating the tertiary structure of proteins. (utmb.edu)
The Jasco J-815 Circular Dichroism Spectrometer housed in the SBL allows for the quick and easy determination of secondary and tertiary structures of protein samples. (utmb.edu)

In Nature , a team from the US, Japan, France, and Israel outlines a strategy for measuring thermodynamic folding stability across 'mega-scale' protein domain sets. (genomeweb.com)
Our studies aim to explore whether Cys residue pairs in polypeptide strands can stabilize (in the thermodynamic sense) β-sheets formation. (elte.hu)
Thermodynamic data such as protein stability parameters and binding constants can also be obtained. (utmb.edu)
Recently, several mass spectrometry-based proteomics techniques have been developed for the large-scale analysis of thermodynamic measurements of protein stability. (duke.edu)
This has created the possibility of characterizing disease states via differential thermodynamic stability profiles. (duke.edu)
Thermodynamic stability profiles were generated for 809 proteins in brain cell lysates from C57BL/6 mice at age 6- (n=7) and 18-months (n=9). (duke.edu)
Comparative analyses on thermodynamic stability profiles of ~470 proteins revealed relatively few differences. (duke.edu)
The results of this thesis help validate the use of thermodynamic stability measurements to capture disease-related proteomic differences in mice. (duke.edu)

A key component in the virulence of MERS-CoV is the Spike (S) protein, which binds with the host membrane receptor dipeptidyl peptidase 4 (DPP4). (frontiersin.org)

Training in the relevant theoretical and experimental methods described for the study of protein structure and dynamics. (lu.se)
Protein dynamics studied by NMR spin relaxation. (lu.se)
Protein dynamics studied by NMR. (lu.se)

Comparison with simulations on bacterial proteins suggests that the effect of cholesterol is much less pronounced for the latter than for the mouse protein. (preprints.org)
A meta-analysis of 22 studies of bacterial contamination rate estimates for apheresis (AP), platelet-rich plasma (PRP), and buffy coat (BC) collection methods found an overall mean contamination rate of 0.51 per 1000 components (95% confidence index [CI], 0.38-0.67). (medscape.com)

The mutation changes a single protein building block (amino acid) in the slow isoform of myosin binding protein C. Specifically, the mutation replaces the amino acid tryptophan with the amino acid arginine at protein position 236 (written as Trp236Arg or W236R). (medlineplus.gov)
The second PD mouse model studied was one in which the human α-synuclein protein, containing the known PD mutation A53T, was overexpressed. (duke.edu)
The mutation responsible for the inherited form of ALS is known to originate in the gene encoding the TDP-43 protein, but the relationship between the biochemical properties of TDP-43 and the progression of ALS has been unclear. (medicalxpress.com)

Several of the amino acid substitutions on the VP7 and NSP4 proteins were identified in regions known to influence function and may have contributed to the emergence and increased dominance of the outbreak strains. (cdc.gov)
It can be very misleading to simply assume that conservative amino acid substitutions cause small changes in stability, whereas large stability changes are associated with nonconservative replacements. (rcsb.org)

Using their high-throughput complementary DNA (cDNA) display proteolysis assay, the investigators report they can gauge protein folding stability patterns in a library of up to 900,000 sequences per week with a reagent price tag of around $2,000. (genomeweb.com)
In comparison, mRNA vaccines can be more quickly generated in the laboratory using the genetic sequences for selected pathogen proteins. (cdc.gov)

In addition to the use of chemical labeling methods to study structure and function of proteins in vitro and in vivo, chemoselective conjugation techniques are also used to functionalize artificial protein scaffolds, such as viral capsids [5-7] . (beilstein-journals.org)
It covers degradation and experimental methods to study denaturation, and evaluates stabilizers and the use of stabilizing excipients. (pda.org)
Using a model of heat-labile proteins, Ab to phospholipase A2, and a sensitive detection system for Ab binding (ELISA), a pilot steam chamber was designed and constructed to develop methods of encapsulating proteins. (wisconsin.edu)
Lectures and computer-based exercises covering biotechnological methods and the structure and function of proteins. (uu.se)
Bioanalysis for large molecules, like Proteins or specific Biotherapeutics, refer to a set of methods, assays, and procedures that enable scientists to analyze specific proteins found in living organisms and the biochemical reactions underlying life processes. (sigmaaldrich.com)
We also aim at an understanding of the forces that underpin the three-dimensional structure of proteins, as well as a basic understanding of the methods used in structure-based drug design. (lu.se)
demonstrate basic knowledge of how nuclear magnetic resonance (NMR), neutron diffraction and small-angle X-ray and neutron scattering can be used to produce specific information in addition to X-ray crystallography · assimilate and critically evaluate the scientific literature dealing with protein structure and function, particularly in terms of the experimental methods. (lu.se)

Theoretically, he suggested, the same might happen in sporadic ALS, if some other mechanism enhanced the stability of wild-type TDP-43. (alzforum.org)
The main body of this project has been dedicated to investigating the effect of key formulation parameters, such as pH, ionic strength and temperature, on the stability and the aggregation mechanism of a model protein, Glucose oxidase at low (1 mg/ml) protein concentration. (bl.uk)
The results revealed that the rate and the mechanism of inactivation is pH, ionic strength, temperature, surfactant and protein concentration dependent. (bl.uk)
This study reveals a novel mechanism linking .O(-)(2) with Bcl-2 stability and provides a new dimension to ROS-mediated Bcl-2 downregulation and apoptosis induction. (cdc.gov)
Yamanaka's group found that stabilized TDP-43 protein molecules inhibit proteasome activity, thus adding to the growing body of evidence that this clearing mechanism fails in neurodegenerative diseases . (medicalxpress.com)

External perturbations such a change in pH, ionic strength and temperature were used to probe the response of proteins, both at low and high concentrations. (ku.edu)

Studies are currently underway in humans and mouse models. (medscape.com)
Using the variation that exists between other species of mammals you can get much closer to identifying other changes that are in the nature of longevity that may not differentiate us significantly at the genetic level between humans," adds study co-director Gerard Muntané. (studyfinds.org)
NIH MedlinePlus Magazine had a chance to chat with Dr. Hodes about the latest in cutting-edge Alzheimer's disease research, geroscience (which focuses on the intersection of basic aging biology, chronic disease, and health), the importance of diversity in research, how studying dogs can help us learn about-and improve-health and longevity for both humans and our canine friends, and more. (medlineplus.gov)

Strong extrinsic CD bands of the extrinsic chromophore and intrinsic CD signals of protein can also be used to monitor protein-ligand interaction. (utmb.edu)
Our Surface Plasmon Resonance (SPR) Biacore T100 biosensor instrument is a high-throughput equipment that allows the study of protein-protein and protein-ligand kinetics and thermodynamics. (utmb.edu)
They further suggest that the stability of the complex can be improved by suppressing ligand dissociation by using late-transition-metal complexes for binding lysozyme, increasing interaction between the complexes and lysozyme by using ligands with hydrogen-bonding moieties, and introducing acidic functional groups to counter the basic side chains of lysozyme. (tus.ac.jp)
Includes protein crystallization, data collection at MAX IV, data processing, structure determination and modelling, as well as a simple exercise in ligand docking. (lu.se)

The stability of emulsion formulations may be enhanced by including sugar beet pectin in addition to whey protein, with potential for encapsulation of value-added ingredients, says a new study. (foodnavigator.com)
This is evident from the observation that · proteins at very high concentrations respond to formulations in a very different way than the same proteins at low concentrations. (bl.uk)
The findings from the work obtained from highly concentrated formulations showed the need for development of techniques that directly measure the stability of proteins at high concentrations without a dilution step. (bl.uk)

These proteins play essential roles in muscle cell structures called sarcomeres, which generate the mechanical force needed for muscles to contract. (medlineplus.gov)
Small-angle neutron scattering was employed to characterize protein structures in both liquid and frozen solution states. (nist.gov)
We compared the S protein structures of MERS-CoV, SARS-CoV, and SARS-CoV-2 viruses and identified the residues like C526, C383, and N468 located in equivalent positions of these viruses have effects on S protein structure. (frontiersin.org)

Compared with normal amounts, one study found only 50% of the profilaggrin mRNA and 10% of the profilaggrin protein present. (medscape.com)
Research has shown that defective posttranscriptional regulation leads to decreased stability of profilaggrin mRNA. (medscape.com)
IGF2BP3 increased the stability of the EIF5B mRNA in an m6A-dependent manner. (techscience.com)
They also found that stabilized TDP-43 protein loses the ability to control its own mRNA transcripts, thereby further accelerating its accumulation. (medicalxpress.com)
mRNA contains the chemical instructions that ribosomes, the protein-making machinery in cells, use to make proteins. (cdc.gov)
Using the genetic code of the SARS-CoV-2 virus, vaccine manufacturers developed mRNA that instructs ribosomes to produce the spike protein of the SARS-CoV-2 virus. (cdc.gov)
First proposed in 1989, mRNA vaccines have been studied for years, with several ongoing clinical trials using mRNA vaccines for cancer and viral diseases, including rabies, influenza, and Zika . (cdc.gov)
For vaccine mRNA to deliver the instructions for making the spike protein, it needs to be able to enter the cells so that the protein-making machinery can access it. (cdc.gov)

This provides evidence that SOD1 stability and its aggregation propensity are the main toxic causes of ALS. (genengnews.com)

Analytical ultracentrifugation studies of cytochrome p450 orthologues led to the successful crystallization and structure solution of the first open-form of a p450 ( 1 ). (utmb.edu)
In this presentation, we will show results of our approach for the hydration of water, of a peptide model in different osmolyte mixtures, and finally for the protein cytochrome C. The potential utility of this approach for studying ion and osmolyte-specific effects in protein solution thermodynamics will be indicated. (aiche.org)

This method was applied to the conjugation of biotin and β-linked galactose residues to yield an enzymatically active thermophilic lipase, which revealed specific binding to Erythrina cristagalli lectin by SPR binding studies. (beilstein-journals.org)
The model (containing two strands of chiral amino acid residues) allows the possibility to form either a single or double inter- or intra-strand disulfide bridges and to assess the stability of β-strand foldamers as function of disulfide bridges and their oxidation states via a QM based description, heading to explain the intrinsic feature of polypeptide aggregation. (elte.hu)

In both studies, mechanisms by which AQP5 altered these proteins were not further elucidated. (plos.org)
The mechanisms by which they regulate the stability and activity of p53 are not fully established. (rcsb.org)
In this study, we investigated the functional role of eukaryotic initiation factor 5B (EIF5B) in hepatocellular carcinoma (HCC) and the underlying mechanisms. (techscience.com)
The cell culture experiments provide a new model that can be used to control the stability of TDP-43, and which could provide further insights into the importance of protein stability for the mechanisms of disease development and progression. (medicalxpress.com)

Studies suggest that myosin binding protein C contributes to the stability and maintenance of sarcomeres. (medlineplus.gov)

Far-UV CD (190-250 nm) is used to measure the secondary structure of proteins, which monitors any change in the peptide backbone. (utmb.edu)
Protein secondary and three- dimensional structure. (lu.se)

On the basis of products, the protein stability analysis market is divided into reagents & assay kits, instruments, consumables & accessories, and software. (marketsandmarkets.com)
The large share of this market segment can be attributed to the high consumption of reagents and assay kits in protein stability analysis techniques, increasing protein pharmaceutical R&D, and increased government funding for life science research. (marketsandmarkets.com)
Changes in the physical state of the model protein were monitored using a combination of different techniques, including activity assay, Size-exclusion chromatography, visual assessment, intrinsic and extrinsic fluorescence spectroscopy, and static light scattering. (bl.uk)

Surveillance studies and serum antibody studies indicate that all young children are likely to have had at least one rotavirus infection by the time they are 3 years of age. (cdc.gov)

Intrinsically disordered proteins and their contraction and folding can be monitored using this technique. (utmb.edu)
eLife, 2016) implied that the N-terminus of the magnesium transporter A (MgtA) protein (from hereon called Keif) is intrinsically disordered, but the advantage of this disordered feature to the function of the protein is still unknown. (lu.se)

RRP12 is a crucial nucleolar protein that regulates p53 activity in osteosarcoma cells. (nih.gov)

The protein filaggrin is important in maintaining effective skin barrier function. (medscape.com)
A new study in mice shows that this enzyme continues to be activated and function normally, even without a chemical modification previously thought to be necessary. (nih.gov)
In order to investigate the role of this motif in the structure and function of sHsps, we have made constructs deleting this sequence from alpha A- and alpha B-crystallin, overexpressed, purified, and studied these engineered proteins. (nih.gov)
We believe that a protein is more stable when it continues to perform its function longer within the cell without degradation. (studyfinds.org)

Protein-based therapeutics have outstanding potential for the treatment of diseases, yet their physical and ' chemical protein instability has remained a major barrier for their rapid commercialisation. (bl.uk)
In addition, due to a strong trend in the pharmaceutical industry to formulate protein therapeutics at higher concentrations, key experiments were carried out at high (100 mg/ml) protein concentration. (bl.uk)

Our approach relies on the selective introduction of two different functional moieties in a protein by mutually orthogonal copper-catalyzed azide-alkyne cycloaddition (CuAAC) and oxime ligation. (beilstein-journals.org)
We investigated the formation of a hybrid protein composed of lysozyme and a functional SOD-mimetic Cu(II) complex. (tus.ac.jp)
We theorized that the resulting SOD-mimetic hybrid protein would improve the biocompatibility and stability of the functional SOD model Cu(II) complex," explains Dr. Nakane as the rationale behind their study. (tus.ac.jp)

Protein drugs, such as monoclonal antibodies, have proved successful in treating cancer and immune system diseases. (lu.se)

Compared with mass spectrometry-based high-throughput stability assays, cDNA display proteolysis achieves a 100-fold larger scale and can easily be applied to study mutational libraries that pose difficulties for proteomics," the authors report. (genomeweb.com)

With a very high molar proportion of fatty acid to saccharide when compared to the other studies, the authors suggested that increasing the proportion of fatty acid could negatively affect the lipase conformation due to an acidification of the reaction medium. (researchgate.net)
Basic knowledge of protein structure: polypeptide conformation. (lu.se)

Differential scanning fluorimetry (DSF) that measures the intrinsic fluorescence from your protein (Tryptophans and Tyrosines), hence no reporter dye is needed. (lu.se)

Researchers in Human Genetics and Genomics Advances report that how researchers describe genomic studies may alienate potential participants. (genomeweb.com)

To find clues to the protein's normal role, the scientists isolated the wild-type protein, with its associated hangers-on, and used mass spectrometry to identify the partners. (alzforum.org)

ATRX proximal protein associations boast roles beyond histone deposition. (nih.gov)

A-TEEM has already proven in many cases to be more effective in protein, vaccine, wine and water research, quality and process applications than HPLC and vibrational spectroscopy. (horiba.com)

Such unique hybrid biocompatible metal-protein SOD complexes may be viable low-toxicity alternatives in therapeutic drug applications. (tus.ac.jp)
The study introduces a new class of SOD active hybrid protein complexes that are biocompatible and have no side reactions with bodily fluids after decomposition of the mimetic complex. (tus.ac.jp)

We chose lysozyme owing to its stability and crystallinity. (tus.ac.jp)
Through assays, the researchers established high SOD activity and stability of the biocompatible CuST@lysozyme hybrid protein complex. (tus.ac.jp)

As a biologically important example we have studied the complex formed by cyclins and cyclin-dependent kinases (CDKs), which play an essential role in the control of the eukaryotic cell cycle. (lu.se)

The latter possibility can help address fundamental questions regarding the thermodynamics that govern the structure and stability of proteins. (aiche.org)

Anatomy12

Organisms8

Diseases1

Chemicals and Drugs106

Analytical, Diagnostic and Therapeutic Techniques and Equipment38

Phenomena and Processes87

Disciplines and Occupations3

Technology, Industry, Agriculture1

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Interactions14

Structural11

Peptides2

Mutant10

Genome3

Mutations8

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Molecular7

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Conformational4

Genes5

Spectroscopic3

Capsid3

Tertiary2

Thermodynamic8

Membrane1

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Sequences2

Methods7

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Humans3

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Formulations3

Structures3

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Cytochrome2

Residues2

Mechanisms4

Contributes1

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Assay3

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