Study microdialysis. Medical search. Frequent questions

MDMA (30 mg/kg, i.p.), given three times at 3-h intervals, produced a rapid increase in striatal dopamine release measured by in vivo microdialysis (maximum increase to 380 +/- 64% of baseline). (erowid.org)
This study was performed to evaluate quantitatively the brain distribution and the efflux transport across the blood-brain barrier of probenecid, using in vivo microdialysis and in situ brain perfusion techniques. (aspetjournals.org)
The aim of this study was to develop, for the first time in the human spinal dorsal horn (DH), an in vivo method for the study of amino acids (AAs). (thejns.org)
For that purpose, we used in vivo microdialysis in freely moving mice. (helsinki.fi)
Using in vivo microdialysis, release of ACh was measured in the hippocampus while rats learned and were tested on an amygdala-dependent CPP task and a hippocampus-dependent spontaneous alternation task. (jneurosci.org)
In vivo microdialysis confirmed that 5-HT2 receptor blockade with MDL 100,907 attenuated MDMA-induced increases in extracellular concentrations of striatal dopamine. (erowid.org)
Gamma-aminobutyric acid is released in the dorsal horn by electrical spinal cord stimulation: an in vivo microdialysis study in the rat. (thieme-connect.com)
We examined the effect of three months of rosiglitazone treatment (4 mg b.i.d.) on whole-body insulin sensitivity and in vivo peripheral adipocyte insulin sensitivity as assessed by glycerol release in microdialysis from subcutaneous fat during a two-step (20 and 120 mU · m −2 · min −1 ) hyperinsulinemic-euglycemic clamp in nine type 2 diabetic subjects. (diabetesjournals.org)
We are using complementary in vivo(behavioral) and in vitro (brain slice) approaches to study this problem. (kent.edu)
In this study, in vitro soil and in vivo genetically modified corn models were used to establish the proof of concept that the inclusion of clay sorbents in contaminated soils will reduce the bioavailability of GLP and AMPA in soils and their adverse effects on plant growth. (bvsalud.org)
Results: We examined R6/1 mice at 18-19 weeks of age using in vivo microdialysis. (lu.se)

The direction of the analyte flow is determined by the respective concentration gradient and allows the usage of microdialysis probes as sampling as well as delivery tools. (wikipedia.org)
The molecular weight cutoff of commercially available microdialysis probes covers a wide range of approximately 6-100kD, but also 1MD is available. (wikipedia.org)
Microdialysis probes can consequently be calibrated by either measuring the loss of analyte using drug-containing perfusate or the gain of analyte using drug-containing sample solutions. (wikipedia.org)
The use of probes with large pore size membranes required for the microdialysis of large molecules creates some methodological challenges such as protein aggregation, protein adhesion, ultrafiltration and low fluid recovery/extraction efficiency. (harvardapparatus.com)
CMA offers a set of microdialysis probes for the recovery of large molecules (such as proteins, cytokines, growth factors, antibody and neuropeptides…) on awake rodents. (harvardapparatus.com)
Before making microsurgical lesions, specific concentric-type microdialysis probes were implanted over a 60-minute period in the DREZ and directed to the DH apex (10 implantations). (thejns.org)
Microdialysis - originally developed for use in neuroscience and pharmacokinetics - is a new technique that uses small probes to sample compounds dissolved in soil solution (Figure 1), without the need to dig up soil and potentially alter its chemical properties. (bgs.ac.uk)
The placements of microdialysis probes were verified from fixed brain sections after the experiments. (helsinki.fi)
Microdialysis probes recoveries were determined by retrodialysis. (ufrgs.br)

The microdialysis technique requires the insertion of a small microdialysis catheter (also referred to as microdialysis probe) into the tissue of interest. (wikipedia.org)
The microdialysis probe is designed to mimic a blood capillary and consists of a shaft with a semipermeable hollow fiber membrane at its tip, which is connected to inlet and outlet tubing. (wikipedia.org)
Due to the constant perfusion of the microdialysis probe with fresh perfusate, a total equilibrium cannot be established. (wikipedia.org)
In theory, the extraction efficiency of a microdialysis probe can be determined by: 1) changing the drug concentrations while keeping the flow rate constant or 2) changing the flow rate while keeping the respective drug concentrations constant. (wikipedia.org)
A concentric microdialysis probe was inserted into the optic nerve head of a rabbit and was perfused with Ringer's solution at a constant flow rate of 2 microl/min. (nih.gov)
CMA 7 55 kDa Microdialysis Probe, 1 mm membrane length, pkg. (harvardapparatus.com)
CMA 8 High Cut-Off Microdialysis Probe, 1 mm membrane length (pkg. (harvardapparatus.com)
We recommend the use of the CMA Microdialysis CNS Perfusion fluid with Dextran 500 kDa 3% in order to stabilize the pressure within the probe and limit ultra-filtration. (harvardapparatus.com)
In the microdialysis experiment, the mice received amphetamine stimulation (100 µM/60 min) via microdialysis probe. (helsinki.fi)

One reference catheter (10-mm membrane, perfused with Ringer's solution at 0.3 µl/minute) was constant for all studies. (thejns.org)
Recent work by Shah et al using a microdialysis catheter has shown an increase in biochemicals associated with pain, including protons (a more acidic environment), inflammatory mediators, neuropeptides, cytokines, and catecholamines in the tissue around active trigger points. (medscape.com)

Rapid online-SPE-MS/MS method for ketoprofen determination in dermal interstitial fluid samples from rats obtained by microdialysis or open-flow microperfusion. (joanneum.at)
The presented method was applied to dermal interstitial fluid samples obtained in a topical administration study of ketoprofen in rats. (joanneum.at)
In the present study, release of ACh in the hippocampus was measured while rats were trained and tested on the amygdala-dependent CPP task, as well as during testing on a hippocampus-dependent spontaneous alternation task. (jneurosci.org)
Purpose: The objective of this study was to model the killing effect of a β-lactam antibiotic, piperacillin (PIP), in neutropenic and E. coli ATCC 25922 infected rats after different dosing regimens using a modified Emax PK-PD model. (ufrgs.br)
Methodology: Pharmacokinetic studies: Total plasma and free tissue concentrations of PIP, determined by microdialysis, were investigated after i.v. bolus of 240 mg/kg of the drug to immunecompromised (cyclophosphamide) and E. coli infected (107 CFU) Wistar rats. (ufrgs.br)
Pharmacodynamic studies: The infected rats were treated with iv bolus PIP 120 mg/kg or 240 mg/kg q8h, q6h, q4h. (ufrgs.br)
SUVN-G3031 was evaluated in brain microdialysis for evaluation of neurotransmitters like acetylcholine, histamine, dopamine and norepinephrine in male Wistar rats. (atsbio.com)
These results are in agreement with EEG studies carried out in healthy male Wistar rats. (atsbio.com)
The authors have examined the effects of an NO synthase inhibitor and a soluble guanylate cyclase inhibitor on the concentrations of NO metabolites (NO2-/NO3-) and glutamate in the cerebrospinal fluid after intrathecal perfusion of NMDA, concomitantly observing pain-related behavior (scratching, biting, and vocalization) in unanesthetized, free-moving rats using an intrathecal microdialysis method. (silverchair.com)
In this study, researchers exposed adult male rats to amphetamine for two weeks and monitored them for four weeks of withdrawal. (madinamerica.com)
Researchers administered methylphenidate at two doses to rats for 90 days during the developmental period that matches adolescence and young adulthood in humans, and studied the effects on the brain seven days after withdrawal. (madinamerica.com)

Clinical microdialysis enables monitoring of the cerebral extracellular chemistry of neurosurgical patients. (thejns.org)
Further studies are required to delineate putative mechanisms of hyperglycemia induced cerebral harm. (frontiersin.org)
In a retrospective observational study of adult TBI our group has recently demonstrated an association between elevated blood glucose and impaired cerebral pressure-reactivity index (PRx) ( 7 ). (frontiersin.org)
Cerebral microdialysis was performed to obtain semi real time measurements of cerebral metabolic status. (lu.se)

The present study examined the mechanisms by which 3,4- methylenedioxymethamphetamine (MDMA) produces long-term neurotoxicity of striatal dopamine neurones in mice and the protective action of the dopamine uptake inhibitor GBR 12909. (erowid.org)
Recent studies have shed light on the mechanisms by which stress and glucocorticoids affect glutamate transmission, including effects on glutamate release, glutamate receptors and glutamate clearance and metabolism. (nature.com)
We further used microdialysis technique to study the underlying mechanisms for the impaired MDMA reward produced by the12-week exercise pre-exposure. (erowid.org)
23 Linderoth B. Dorsal Column Stimulation and Pain: Experimental Studies of Putative Neurochemical and Neurophysiological Mechanisms. (thieme-connect.com)

Microdialysis is a minimally-invasive sampling technique that is used for continuous measurement of free, unbound analyte concentrations in the extracellular fluid of virtually any tissue. (wikipedia.org)
However, there was a difference between the dopamine outputs in days 1 and 4 in both MEN2B and GDNF-cKO mice: The striatal dopamine concentrations were significantly lower on the second microdialysis day. (helsinki.fi)

Between microdialysis days, the mice were given amphetamine 1 mg/kg i.p. on days 2 and 3. (helsinki.fi)

Two adjacent microdialysis catheters that were identical in membrane length, perfusion fluid, and flow rate showed equivalent results. (thejns.org)

This clinical bridging study compared the pharmacokinetics (PK) of selatogrel between the different formulations. (bvsalud.org)

The microdialysis principle was first employed in the early 1960s, when push-pull canulas and dialysis sacs were implanted into animal tissues, especially into rodent brains, to directly study the tissues' biochemistry. (wikipedia.org)
In the present study, SUVN-G3031 was evaluated in brain microdialysis and rodent models of electroencephalography (EEG). (atsbio.com)

The proper selection of an appropriate calibration method is critically important for the success of a microdialysis experiment. (wikipedia.org)

Using chemogenetics and microdialysis, we show that, in sign-trackers, stimulation of the neuronal pathway from the prelimbic cortex (PrL) to the paraventricular nucleus of the thalamus (PVT) decreases the incentive value of a reward-cue. (elifesciences.org)

Microdialysis offers advantages over the skin blister and tissue homogenate studies historically used to define antibiotic penetration in skin and soft-tissue infections by defining antibiotic penetration into the interstitial fluid over the entire concentration versus time profile. (japmaonline.org)

Their norepinephrine and serotonin levels in the dentate gyrus of the brain were reduced immediately following treatment, showing evidence of reduced neurogenesis, or new neuron growth, but the levels did not remain low by the end of the study. (madinamerica.com)

Despite the potential advantages of microdialysis, considerable effort is required to develop and progress the analytical chemistry and theoretical frameworks to apply the technology to soil solution multi-elemental analysis. (bgs.ac.uk)

While water-soluble compounds generally diffuse freely across the microdialysis membrane, the situation is not as clear for highly lipophilic analytes, where both successful (e.g. corticosteroids) and unsuccessful microdialysis experiments (e.g. estradiol, fusidic acid) have been reported. (wikipedia.org)

A microdialysis technique was used to sample AAs in the extracellular fluid of the DH apex in eight patients in whom surgery in the dorsal root entry zone (DREZ) was performed. (thejns.org)

Please send study citations that are relevant to the withdrawal literature for stimulants to [email protected] . (madinamerica.com)
Based on the present study and previous literature that we review here, we suggest that a dysfunctional nigrostriatal dopaminergic pathway is likely to contribute to some of the symptoms associated with HD. (lu.se)

After intravenous administration of theophylline, microdialysis has been used for studying the non protein bound theophylline concentration in blood and in lung tissue in the rat as well as in two horses. (slu.se)
The use of the microdialysis technique for the recovery of large molecules is of increasing interest as it offers the opportunity to identify patterns of protein expression in a variety of tissue spaces as well as to evaluate useful biomarkers of disease. (harvardapparatus.com)

The results of this study support the hypothesis that the activation of NMDA receptors modulated pain-related behavior via an NO/cGMP/glutamate release cascade within the spinal cord. (silverchair.com)
3,4 Immunohistochemical studies demonstrated that high densities of neuronal NOS were found in neuronal fibers and interneurons in superficial layers of the dorsal spinal cord. (silverchair.com)
11,12 Furthermore, electrophysiologic studies have shown that either NO or cGMP are involved in the sensitization of spinal secondary neuron after peripheral inflammation. (silverchair.com)

Microdialysis guide cannula was implanted in the dorsal striatum in the stereotaxic surgery and the mice were allowed to recover for 5-7 days. (helsinki.fi)

BGS's Inorganic Geochemistry Facility is at the forefront of developing microdialysis as a soil sampling technique, with the ability to assess rapid chemical reactions with unparalleled temporal and spatial resolution without significantly disturbing in situ physicochemical soil properties. (bgs.ac.uk)
We aim to establish microdialysis as a tool capable of assessing fine-scale, rapid soil chemistry interactions to better inform existing geochemical models that influence phytoremedial and crop biofortification strategies globally, and look forward to updating you in the future as this exciting technique is developed further. (bgs.ac.uk)

To study DAT's function, several genetically modified mouse lines including DAT knockout mice, DAT knockdown mice and DAT knock in mice with elevated DAT levels have been generated. (helsinki.fi)
Our results substantiate the use of a forward-genetics approach for studying sleep behaviours in mice, and demonstrate the role of SIK3 and NALCN in regulating the amount of NREMS and REMS, respectively. (nature.com)
1997. Genotoxicity of malathion- a sub-chronic study in mice. (cdc.gov)
2002. Cytogenitic studies on the effect of feeding mice with stored wheat grains treated with malathion. (cdc.gov)
We are using a variety of technical approaches for the study of SCN pacemaker function ranging from the molecular to the behavioral levels in Syrian hamsters and mice. (kent.edu)
In this study, we have further characterized deficits in the regulation of the nigrostriatal dopaminergic pathway in R6/1 transgenic HD mice. (lu.se)

So, it is very important to study on the relationship between brain edema and ICH. (karger.com)
In recent years, many studies focus on the mechanism of secondary inflammation that can cause brain edema and this may provide new therapy targets for ICH [ 7 ]. (karger.com)
Summary: Numerous studies have demonstrated that brain histamine plays a crucial role in maintenance of wakefulness, attention, learning and other cognitive processes. (atsbio.com)
Objectives: The purpose of this study is the development of a porcine model of carbon monoxide (CO) poisoning to investigate alterations in brain and heart mitochondrial function. (lu.se)
However, in general, the animal studies on stimulant drug withdrawal strongly suggest that the drug exposure leads to a number of both short-term and long-lasting behavioral effects and brain changes. (madinamerica.com)

Previous studies have shown that the nigrostriatal dopaminergic system is impaired in transgenic mouse models of HD. (lu.se)

Pharmacokinetic studies of topical ketoprofen formulations using continuous sampling techniques such as microdialysis (MD) or open-flow microperfusion (OFM) require sensitive assays due to small sample volumes. (joanneum.at)
An easy sample workup procedure was used which enables high throughput analysis of a large number of samples for pharmacokinetic studies. (joanneum.at)
First in human, Phase 1 studies for SUVN-G3031 are completed under US IND and SUVN-G3031 has shown desirable pharmacokinetic profile with safety and tolerability in healthy human volunteers. (atsbio.com)

The aim of this study was to investigate GDNF's role and mechanism of action in plasticity and function of the dopaminergic neurons projecting to striatum. (helsinki.fi)

We have since been awarded a Royal Society of Chemistry grant to continue this research, and have developed a project aimed at establishing harmonised methods for both microdialysis sampling in soils and subsequent data interpretation. (bgs.ac.uk)

5,6 Behavioral studies have shown that the inhibition of either intrathecal NOS or sGC blocks hyperalgesia induced by inflammation and nerve injury but does not alter physiologic nociceptive responses. (silverchair.com)
One aim of the research is to study how environmental information is integrated by the circadian clock to regulate the timing of daily behavioral, physiological and neuroendocrine rhythms. (kent.edu)

The present study demonstrates that microdialysis can be performed safely in the human DH during DREZ lesioning. (thejns.org)

Why large molecules in microdialysis research? (harvardapparatus.com)
1 This study was supported in part by a Grant-in-Aid for Scientific Research provided by the Ministry of Education, Science and Culture of Japan. (aspetjournals.org)
Matthew Ogley, a placement student from the University of Surrey, is currently working in the Inorganic Geochemistry facility to support the research efforts being made on microdialysis, as well as undertaking routine analyses in the laboratories. (bgs.ac.uk)
There is an abundance of animal research on the effects of withdrawal from stimulant drugs, mainly in the form of rat studies. (madinamerica.com)
My research career has been unadventurous in the sense that I have remained at the same institution, Lund University in Sweden, where I started my medical studies in 1964. (lu.se)

The aim of our study was to identify molecular changes produced by prenatal exposure to WIN 55,212-2 that might contribute to late disruption in synaptic plasticity and cognition. (researchgate.net)

A porcine study of ultrasound-guided versus fluoroscopy-guided placement of endovascular balloons in the inferior vena cava (REBOVC) and the aorta (REBOA). (oru.se)
A randomized porcine study of hemorrhagic shock comparing end-tidal carbon dioxide targeted and proximal systolic blood pressure targeted partial resuscitative endovascular balloon occlusion of the aorta in the mitigation of metabolic injury. (oru.se)

Even if you are not persuaded by the studies you've read that Lexapro has better efficacy. (dr-bob.org)

CMA offers new complete solutions for the study of large molecules using the microdialysis techniques. (harvardapparatus.com)

The second aim is to study the effects of ethanol on the circadian timing system. (kent.edu)
Effects of chemical concentration (1-10 mg/kg), sorbent dose (0.5%-3% in soil and 0.5%-1% in plants) and duration (up to 28 days) on sorption kinetics were studied. (bvsalud.org)

A similar result was observed in a clinical study [ 9 ]. (karger.com)

We first began developing the application of microdialysis during Olivier Humphrey's PhD (completed in 2019) at the Centre for Environmental Geochemistry (BGS/University of Nottingham), where he was investigating Short-term iodine soil interactions following a simulated natural/anthropogenic iodine addition event (full paper). (bgs.ac.uk)
The use of microdialysis to investigate soil-elemental speciation dynamics has been further developed by Elliott Hamilton during his PhD (completed in 2020), which will be summarised in a future blog. (bgs.ac.uk)

The neural elements of the clock are confined to the suprachiasmatic nuclei (SCN) of the anterior hypothalamus, which offers an anatomically-discrete model for studying integrative processes regulating specific aspects of circadian rhythms. (kent.edu)

The whole body autoradiography study in rat showed that the concentration of radioactivity in the lung followed the blood concentration very closely up to 24 h after injection. (slu.se)

More recently, several studies have shown that GDNF can also negatively regulate the actions of abused drugs. (helsinki.fi)

METHODS: This was a single-center, randomized, open-label, three-period, cross-over Phase I study in 24 healthy subjects. (bvsalud.org)
Historically, these methods have involved the extrapolation of toxicity data ª generally from studies in laboratory animals at relatively high doses to human exposures at lower doses. (cdc.gov)

This discovery of the electroencephalogram (EEG), as it is now known, made the objective study of sleep possible. (scholarpedia.org)

Many of these studies focus on the effect of medications during adolescence on later drug abuse, with contradictory findings. (madinamerica.com)

In this study we sought to identify predictors for early worsening after SAH and to determine the impact of worsening on outcome. (bmj.com)

Larger scale studies take more time and will out soon too. (dr-bob.org)

It is possible that the differences between genotypes are too small to be seen with microdialysis. (helsinki.fi)

This review addresses studies seeking experimental confirmation of the author's visceral theory of sleep, along with independent investigations whose results are consistent with this hypothesis. (researchgate.net)

This is true of both the animal studies and human studies. (madinamerica.com)

The recovery can be determined at steady-state using the constant rate of analyte exchange across the microdialysis membrane. (wikipedia.org)

Anatomy12

Organisms3

Diseases1

Chemicals and Drugs26

Analytical, Diagnostic and Therapeutic Techniques and Equipment9

Psychiatry and Psychology3

Phenomena and Processes10

Health Care2

Vivo11

Probes9

Probe9

Catheter2

Rats11

Cerebral4

Mechanisms4

Concentrations2

Amphetamine1

Perfusion fluid1

Pharmacokinetics1

Rodent2

Experiment1

Stimulation1

Interstitial fluid1

Serotonin1

Advantages1

Freely1

Extracellular1

Literature2

Tissue2

Spinal3

Dorsal1

Technique2

Mice6

Brain5

Nigrostriatal1

Pharmacokinetic3

Dopaminergic1

Chemistry1

Behavioral2

Present study1

Research5

Synaptic1

Porcine2

Efficacy1

Techniques1

Effects2

Clinical study1

Soil2

Offers1

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Shown1

Methods2

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Time1

Small1

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