• After the biochemical identification tests and the use of VITEK-2 Compact (Biomerieux, France) automated microbial identification system, pathogenic bacteria was identified as Comamonas testosteroni . (imjsu.org)
  • There has been an increase in bacterial infections caused by Comamonas testosteroni and antibiotic resistance of the bacteria. (imjsu.org)
  • The intention of this study was to identify the bacterial pathogens infecting Oreochromis niloticus (Nile tilapia) and Clarias gariepinus (African catfish), and to establish the antibiotic susceptibility of fish bacteria in Uganda. (e-fas.org)
  • This observed resistance is most probably intrinsic to those bacteria, suggesting minimal levels of acquired antibiotic resistance in fish bacteria from the study area. (e-fas.org)
  • The aim of this study was to evaluate microbial culture outcomes and antibiotic susceptibilities of these microorganisms in samples obtained intraoperatively from pediatric patients with appendicitis, and to define the infectious microorganisms responsible for the community-based intra-abdominal infections in our region, and their antibiotic susceptibilities. (turkishjournalpediatrics.org)
  • In this study, using Comamonas testosteroni as a model organism, we demonstrated the involvement of Comamonas biofilms in denitrification under bulk aerobic conditions and elucidated the influence of nitrate respiration on its biofilm lifestyle. (ntu.edu.sg)
  • Under bulk aerobic condition, biofilms of C. testosteroni were capable of reducing nitrate, and intriguingly, nitrate reduction significantly enhanced viability of the biofilm-cells and reduced cell detachment from the biofilms. (ntu.edu.sg)
  • Taking these factors together, this study reveals that nitrate reduction occurs in mature biofilms of C. testosteroni under bulk aerobic conditions, and the respiratory reduction of nitrate is beneficial to the biofilm lifestyle by providing more metabolic energy to maintain high viability and a higher level of c-di-GMP to reduce cell detachment. (ntu.edu.sg)
  • Using samples collected from the Sooke, Nanaimo, and Cowichan Rivers on Vancouver Island, British Columbia, we show that the E. muelleri microbiome is distinct from the ambient water and adjacent biofilms and is dominated by Sediminibacterium, Comamonas , and unclassified Rhodospirillales. (nature.com)
  • In addition, no study has yet determined if freshwater sponge microbiomes differ from adjacent biofilms. (nature.com)
  • However, whereas pK2.4-EGFP-4 could integrate into the chromosomal DNA of C. testosteroni and knock out the βhsd gene promoter, pBB2.4-EGFP-8 could replicate in C. testosteroni cells as a free plasmid DNA. (idexlab.com)
  • These studies have found that, like marine sponges, freshwater sponges are primarily populated by Proteobacteria, Bacteroidetes, and Actinobacteria, though the resident microbial communities still differ substantially from those of marine sponges [ 5 ]. (nature.com)
  • Bacterial KSI also serves as a model protein for studying enzyme catalysis and protein folding. (wikipedia.org)
  • The diversity has been examined of the plasmids and of the gene tdnQ, involved in oxidative deamination of aniline in three bacterial isolates that are able to metabolise both aniline and 3-chloroaniline (3-CA). Strains A and B were isolated in this study from a wastewater treatment plant and were identified as Comamonas testosterone and Delftia acidovorans, respectively. (sciepub.com)
  • Aerobic culture was studied. (imjsu.org)
  • Comamonas testosteroni is a gram-negative, motile, aerobic, non-spore-forming bacillus which is commonly found worldwide. (imjsu.org)
  • Aerobic culture and antibiogram were studied under sterile conditions. (imjsu.org)
  • Identification and isolation of a regulator protein for 3,17β-HSD expressional regulation in Comamonas testosteroni. (idexlab.com)
  • Other proteins studied in the laboratory and whose structure we solved include single-strand binding protein mtSSB (or SSBP1, Piro-Mégy et al. (csic.es)
  • Construction of a biosensor mutant of Comamonas testosteroni for Testosterone determination by cloning the EGFP gene downstream to the regulatory region of the 3,17β-HSD gene. (idexlab.com)
  • Both plasmids contain the EGFP gene fused downstream to a 2.4 kb DNA fragment from C. testosteroni. (idexlab.com)
  • Elucidating the mechanism of 3,17β-HSD gene (βhsd) regulation may help us to generate prospective C. testosteroni mutants for bioremediation. (idexlab.com)
  • Two potential promoters and two repeat sequences (RS, 16 bp), spaced to each other by 1661 bp, were also found upstream of the βhsd gene C. testosteroni. (idexlab.com)
  • In addition, a brp gene knock-out mutant of C. testosteroni was prepared. (idexlab.com)
  • These knock-out mutants showed an enhanced expression of both the βhsd gene and the hsdA gene (the latter coding for 3α-HSD/CR) in the presence of Testosterone . (idexlab.com)
  • Our data reveal that the βhsd gene undergoes complex regulation involving the two promoters, a loop structure via the two repeat sequences, and the steroid Testosterone . (idexlab.com)
  • Comamonas testosteroni is a gram-negative bacillus which commonly occurs in various environments worldwide. (imjsu.org)
  • Comamonas testosteroni is able to catabolize a variety of steroids and polycyclic aromatic hydrocarbons and might be used in the bioremediation of contaminated environments. (idexlab.com)
  • The case recovered from Comamonas testosteroni infection. (imjsu.org)
  • [1] , [3] Thirty-two cases have been reported in the literature concerning C. testosteroni infection so far. (imjsu.org)
  • Based on the results of this study, an idea which immediately suggests itself is that the factors and defence mechanisms of the host immune system may play a fundamental role in the different outcomes of infection. (cret-signal.com)
  • Methods Patient interviews Data for the present study was obtained from PAK5 a prospective cohort study carried out in Denmark from September 2001 to December 2002 [30]. (cret-signal.com)
  • In summary, this new biosensor system might be used for the specific determination of Testosterone . (idexlab.com)
  • Stability study and determination of benzene- and naphthalenesulfonates following an on-line solid-phase extraction method using the new programmable field extraction system. (ncats.io)
  • three of these strains were capable of accumulation of medium chain length PHA (mclPHA) from TA as a sole source of carbon and energy were selected for further study. (epa.ie)
  • Although the investigated strains had different genomic click here contents, this study found no evidence of a correlation between the genomic contents of the S. Typhimurium strains and the symptoms they caused in human cases of salmonellosis. (cret-signal.com)
  • In contrast, in C. testosteroni cells transformed with pBB2.4-EGFP-8 the expression of EGFP was induced with Testosterone . (idexlab.com)
  • In this study, we aimed to investigate the physiology of P4HB synthesis, and to reduce the total production cost by using cheap and widely available xylose as the growth substrate and sodium 4-hydroxybutyrate (Na-4HB) as the precursor for P4HB synthesis. (biomedcentral.com)
  • The effect of growth conditions such as temperature and physiological stage of Na-4HB addition on P4HB synthesis was also studied in E. coli JM109 recombinant in batch culture. (biomedcentral.com)
  • The case we report is the first Comamonas testosteroni endocarditis in Turkey and the fourth case in the world. (imjsu.org)
  • This case report is issued because it is the first endocarditis case in Turkey which C. testosteroni was isolated. (imjsu.org)
  • Our results showed that C. testosteroni could use nitrate as the sole electron acceptor for anaerobic growth. (ntu.edu.sg)
  • Its identity and importance as a proton relay was verified in comparative studies on cofactor variants of HydA1 from Chlamydomonas reinhardtii 21 . (nature.com)
  • A comparative study of intermolecular interactions in the crystal structures of phenyl/phenyl end-capped oligoanilines. (ncats.io)
  • The enzymes from these two sources are 34% homologous, and structural studies have shown that the placement of the catalytic groups in the active sites is virtually identical. (wikipedia.org)
  • The presence or absence of additional S. Typhimurium genes, which are not present in the LT2 sequence, could not be assessed in this study. (cret-signal.com)
  • The present study investigated developmental toxicity of a widely used neonicotinoid, acetamiprid, to zebrafish embryos. (inra.fr)
  • The present study provides the basis for understanding developmental toxicity of acetamiprid exposure to zebrafish embryos. (inra.fr)
  • Utilizing a UPLC-Q-Exactive Orbitrap-MS technique combined with metabolomics, this study was the first to identify S-methyl-L-cysteine sulfoxide (SMCSO) in RRJ, thereby it from other types of RJ. (bvsalud.org)
  • This study included 231 patients between 0 and 16 years of age, operated on due to appendicitis between 2014 and 2017. (turkishjournalpediatrics.org)
  • P4HB biosynthesis has been studied for about 20 years and it was, and still is, the first and only PHA-based product approved by the FDA as an absorbable suture for clinical application. (biomedcentral.com)
  • In a second study they suggested a regulative function for positions R286 and S320 in the PT of CpI 22 . (nature.com)
  • In this study, we revealed the role of ABHD11 in ESC function using classical loss and gain of function experiments. (inra.fr)