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  • Researchers
  • Researchers studying water-soluble proteins often use commercial E. coli -based systems to express, or produce, copies of the protein. (medgadget.com)
  • Researchers will test the reagents on the membrane proteins produced in the Rhodobacter 'factory. (medgadget.com)
  • Last month, the National Institute of General Medical Science launched the Membrane Protein Structural Dynamics Consortium, an interdisciplinary team of researchers that aim to use biophysical and computational methods to understand the structure, function, and dynamics of membrane proteins. (memprotein.org)
  • Along similar lines, Harel Weinstein, a professor of physiology and biophysics at Cornell University, said the group will develop tools for "large-scale simulations" of protein structures that realistically represent the protein and its fluid environment, as well as tools to let researchers look at protein dynamics based on known structures and to interpret "a new generation of data" from things like single molecule experiments. (memprotein.org)
  • In Membrane Transporters in Drug Discovery and Development: Methods and Protocols , expert researchers provide practical methodologies of the ongoing research on membrane transporters, considering applications of transporter technologies in drug discovery and development. (springer.com)
  • Wide-ranging and current, Membrane Transporters in Drug Discovery and Development: Methods and Protocols delivers a collection of practical protocols that can be used immediately in the lab, along with critical surveys of key topics by leading researchers in the field. (springer.com)
  • biophysical
  • The results of this study will be helpful for a variety of biophysical experiments when specific size of lipid disc is required. (acancerjourney.info)
  • She was a member of the CNRS tenure position recruitment committee and is currently a member of the executive committees of the French Biophysical Society and of the Membrane Group Society. (springer.com)
  • amphiphiles
  • Although a large number of novel agents have been reported, so far there has been no systematically designed comparative study of the protein stabilization efficacy of stereo-isomeric amphiphiles. (rsc.org)
  • dynamics
  • In addition, the AFM (located in the Centre for Cell Imaging) is used to explore the assembly dynamics of bacterial microcompartment shell proteins and the formation process of shell facets. (news-medical.net)
  • We have designed and developed an unsupported, horizontally held artificial bilayer and used it successfully in studying the ion-channel protein dynamics by simultaneous single-molecule fluorescence imaging and electric current measurements. (bgsu.edu)
  • The dynamics of the channel transition between open and close states and the translocation of charged ¿¿¿¿-helices across the membrane were studied. (bgsu.edu)
  • How does the crystal packing influence protein dynamics? (ibs.fr)
  • Atomic-resolution studies of membrane proteins generally suffer from the need to extract the protein from its native lipid environment, which can introduce perturbations of structure and dynamics. (ibs.fr)
  • structure
  • Membrane proteins have proven to be very difficult to crystallize through X-ray crystallography mainly due to the large percentage of non-polar amino acids present within the primary structure. (wikibooks.org)
  • That causes the membrane proteins to be more susceptible to their loss of native structure which also results in the deactivation of the protein. (wikibooks.org)
  • Malfunction of specific membrane proteins are the cause for several diseases or disorders.Knowledge of protein structure supports understanding of its mechanism of function. (avhandlingar.se)
  • The solution structure of the PERCTER that emerges from the NMR data contains considerable alpha-helix at the amino terminus (near the membrane) and is disordered at the C-terminus. (arvojournals.org)
  • Recently, technological advances across several disciplines have prompted considerable progress in three-dimensional structure determination of membrane proteins. (schoolbag.info)
  • Most notably, X-ray crystallography will be discussed, as this technique has made by far the largest contribution to our current knowledge of membrane protein structure. (schoolbag.info)
  • The first membrane protein structure solved by X-ray crystallography (XRC) was that of the photoreaction center from Rhodopseudomonas viridis, originally solved at 3 A resolution in 1985 (1). (schoolbag.info)
  • This proposal would determine the structure of proteins in the membrane ofthe tight junction and use this information to elucidate the mechanism by which they function. (grantome.com)
  • Our AFM imaging results reveal the formation of linear aggregates where each monomer is tilted along the aggregated structure in photosynthetic membrane. (bgsu.edu)
  • A new consortium launched with the aim of understanding the structure and dynamic function of membrane proteins will likely result in an improved informatics toolbox for modeling proteins and other biomolecules. (memprotein.org)
  • The planned database will be different from repositories like the Protein Structure Initiative's Structural Biology Knowledgebase in the sense that "the same people who create the tools for dissemination and disseminate the data are the people who collect the data" he said. (memprotein.org)
  • Using the recently determined crystal structure of a prokaryotic leucine transporter (LeuT), the scientists collaborating in this project are modeling the transport mechanisms of these proteins. (memprotein.org)
  • This field has made significant advances since the elucidation of the first 3-D structure of a recombinant G Protein Coupled Receptor (GPCR), rhodopsin, with the structure of several more GPCRs having been solved in the past five years. (springer.com)
  • translocation
  • most of these proteins occurs with the assistance of a In what follows I begin with a simplified de- large complex of translocation/insertion machinery. (deepdyve.com)
  • interact
  • AFM has been used to investigate the supramolecular architecture of photosynthetic membranes in bacteria and algae, revealing how photosynthetic proteins are organized and interact with each other and how the membranes are synthesized to optimize the photosynthetic efficiency in nature. (news-medical.net)
  • Identify new host factors interacting with NS4B - we identified 106 host proteins that interact with NS4B during DENV replication using a novel approach of coupling quantitative stable isotope labeling with amino acids in cell culture mass spectrometry (SILAC MS/MS) with immunoprecipitation (IP). (ntu.edu.sg)
  • 2002
  • 192, 149-157 (2003) The Journal of DOI: Membrane Biology 9 Springer-Verlag New York Inc. 2003 Topical Review R.J. Turner Membrane Biology Section, Gene Therapy and Therapeutics Branch, National Institute of Dental and Craniofacial Research, National Institutes of Health, DHHS, 10 Center Drive MSC 1190, Bethesda MD 20892, USA Received: 17 September 2002 Introduction detail and it is this process that concerns us here. (deepdyve.com)
  • fluorescence
  • An increase in the fluorescence anisotropy (r) of TAMRA-Aβ1-42 in the presence of the lipid-coated AuNPs indicate that the Aβ monomers bind to the membrane surface. (pdx.edu)
  • Cells
  • When membrane proteins are produced in E. coli , they overload the cell's bi-layers and cause the cells to die. (medgadget.com)
  • Cells have receptor proteins in their plasma membranes "listening" to chemical signals from the outside world. (rms.org.uk)
  • The kinetic and thermodynamic aspects of the trimer assembly process are monitored in cells and in reconstituted lipid bilayers using an array of assays and methods developed in preliminary studies. (uky.edu)
  • The concerted actions of the proteins trigger many of the critical activities that occur in living cells. (anl.gov)
  • on membranes and proteins in human pulmonary endothelial cells (HPMECs). (asme.org)
  • successfully
  • The biologists developed a system that successfully expresses hundreds of copies of a chosen membrane protein in Rhodobacter while simultaneously synthesizing the internal membranes they want to live in. (medgadget.com)
  • Structural determination of NS4B - various recombinant NS4B protein constructs were successfully expressed, purified to monodispersity and the yield were sufficient for crystallisation studies. (ntu.edu.sg)
  • 1999
  • It is now less well characterized (Buchanan, 1999) and will not clear that the membrane integration and assembly of be discussed. (deepdyve.com)
  • research
  • The protocols and parameters developed in the research provide valuable tools and benchmarks for the research community, which lead the way to new initiatives in membrane protein research. (uky.edu)
  • modeling and simulation tools are routinely used to fill in knowledge gaps from experiments and they are used to help design and define research studies. (anl.gov)
  • For several years Roux's research has been focused on membrane proteins, which control the bidirectional flow of material and information in a cell. (anl.gov)
  • The project is a part of an ambitious research program that aims on the infrared spectroscopic study of conformational movements in membrane proteins from the respiratory by introducing a -CN label in critical positions. (cnrs.fr)