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  • fluorescence
  • Utilizing trp fluorescence, refolding studies were performed on single trp mutants of OmpA to determine the thermodynamic stability of these trp mutants. (escholarship.org)
  • An increase in the fluorescence anisotropy (r) of TAMRA-Aβ1-42 in the presence of the lipid-coated AuNPs indicate that the Aβ monomers bind to the membrane surface. (pdx.edu)
  • interact
  • AFM has been used to investigate the supramolecular architecture of photosynthetic membranes in bacteria and algae, revealing how photosynthetic proteins are organized and interact with each other and how the membranes are synthesized to optimize the photosynthetic efficiency in nature. (news-medical.net)
  • Identify new host factors interacting with NS4B - we identified 106 host proteins that interact with NS4B during DENV replication using a novel approach of coupling quantitative stable isotope labeling with amino acids in cell culture mass spectrometry (SILAC MS/MS) with immunoprecipitation (IP). (ntu.edu.sg)
  • photosynthetic
  • Our AFM imaging results reveal the formation of linear aggregates where each monomer is tilted along the aggregated structure in photosynthetic membrane. (bgsu.edu)
  • structure
  • Membrane proteins have proven to be very difficult to crystallize through X-ray crystallography mainly due to the large percentage of non-polar amino acids present within the primary structure. (wikibooks.org)
  • That causes the membrane proteins to be more susceptible to their loss of native structure which also results in the deactivation of the protein. (wikibooks.org)
  • Malfunction of specific membrane proteins are the cause for several diseases or disorders.Knowledge of protein structure supports understanding of its mechanism of function. (avhandlingar.se)
  • Peripherin-2 is a tetraspannin transmembrane protein, implicated in retinitis pigmentosa and localized in retinal rod outer segment disk membranes, whose complete 3-dimensional structure is the goal of this nuclear magnetic resonance (NMR) study. (arvojournals.org)
  • The solution structure of the PERCTER that emerges from the NMR data contains considerable alpha-helix at the amino terminus (near the membrane) and is disordered at the C-terminus. (arvojournals.org)
  • Recently, technological advances across several disciplines have prompted considerable progress in three-dimensional structure determination of membrane proteins. (schoolbag.info)
  • Most notably, X-ray crystallography will be discussed, as this technique has made by far the largest contribution to our current knowledge of membrane protein structure. (schoolbag.info)
  • The first membrane protein structure solved by X-ray crystallography (XRC) was that of the photoreaction center from Rhodopseudomonas viridis, originally solved at 3 A resolution in 1985 (1). (schoolbag.info)
  • This proposal would determine the structure of proteins in the membrane ofthe tight junction and use this information to elucidate the mechanism by which they function. (grantome.com)
  • A new consortium launched with the aim of understanding the structure and dynamic function of membrane proteins will likely result in an improved informatics toolbox for modeling proteins and other biomolecules. (memprotein.org)
  • The planned database will be different from repositories like the Protein Structure Initiative's Structural Biology Knowledgebase in the sense that "the same people who create the tools for dissemination and disseminate the data are the people who collect the data" he said. (memprotein.org)
  • Using the recently determined crystal structure of a prokaryotic leucine transporter (LeuT), the scientists collaborating in this project are modeling the transport mechanisms of these proteins. (memprotein.org)
  • scientists
  • The consortium, funded by a five-year $22.5 million "glue grant" from NIGMS, comprises 30 scientists from 14 institutions in four countries and is hailed as one of the largest and most comprehensive projects focused on membrane proteins to date. (memprotein.org)
  • The project is expected to help scientists better understand a wide range of ailments caused by faulty membrane proteins, such as some forms of heart disease, diabetes, and neurological and hormonal disorders. (memprotein.org)
  • successfully
  • The biologists developed a system that successfully expresses hundreds of copies of a chosen membrane protein in Rhodobacter while simultaneously synthesizing the internal membranes they want to live in. (medgadget.com)
  • Structural determination of NS4B - various recombinant NS4B protein constructs were successfully expressed, purified to monodispersity and the yield were sufficient for crystallisation studies. (ntu.edu.sg)
  • methods
  • The kinetic and thermodynamic aspects of the trimer assembly process are monitored in cells and in reconstituted lipid bilayers using an array of assays and methods developed in preliminary studies. (uky.edu)
  • However
  • However, studies of membrane proteins are hindered by several obstacles, including their low ability to crystallize, highly dynamic behavior of some of their domains, and need for membrane-like environment. (degruyter.com)
  • However, like any machine, the components can break (through various mutations) and then the proteins fail to perform their functions correctly. (anl.gov)
  • However, for a large class of partially structured or flexible and dynamic proteins, the methodological problems such as in-homogeneous resonance line broadening which results in partial or complete loss of signal to background noise, may appear insurmountable. (ntu.edu.sg)
  • However, its intrinsic membrane association and non-enzymatic nature has impeded progress in our understanding of NS4B. (ntu.edu.sg)
  • research
  • The protocols and parameters developed in the research provide valuable tools and benchmarks for the research community, which lead the way to new initiatives in membrane protein research. (uky.edu)
  • modeling and simulation tools are routinely used to fill in knowledge gaps from experiments and they are used to help design and define research studies. (anl.gov)
  • For several years Roux's research has been focused on membrane proteins, which control the bidirectional flow of material and information in a cell. (anl.gov)
  • Membrane proteins involved in transport processes are key targets for pharmaceutical research and industry. (cef-mc.de)
  • novel
  • Although a large number of novel agents have been reported, so far there has been no systematically designed comparative study of the protein stabilization efficacy of stereo-isomeric amphiphiles. (rsc.org)
  • large
  • Under certain conditions - in response to light or oxygen - Rhodobacter naturally produces large quantities of internal membranes. (medgadget.com)